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Volume 13 Number 3 A review of DNA repair and possible
... skin cancer. Mismatch repair of DNA is detected through investigation of DNA microsatellites. Microsatellites are repetitive mono-, di-, tri-, or tetra-nucleotide sequences, scattered throughout the human genome, which frequently show length polymorphisms. These repetitive sequences are often locate ...
... skin cancer. Mismatch repair of DNA is detected through investigation of DNA microsatellites. Microsatellites are repetitive mono-, di-, tri-, or tetra-nucleotide sequences, scattered throughout the human genome, which frequently show length polymorphisms. These repetitive sequences are often locate ...
Determining Evolutionary Relationships Using BLAST
... Data from DNA sequencing is of limited use unless it can be converted to biologically useful information. Bioinformatics therefore is a critical component of DNA sequencing . It evolved from the merging of computer technology and biotechnology. The widespread use of the internet has made it possible ...
... Data from DNA sequencing is of limited use unless it can be converted to biologically useful information. Bioinformatics therefore is a critical component of DNA sequencing . It evolved from the merging of computer technology and biotechnology. The widespread use of the internet has made it possible ...
Chpt9_Transposition.doc
... viruses move between individuals, at least some transposable elements can move between genomes (between individuals) as well as within an individual’s genome. Given their prevalence in genomes, the function (if any) of transposable elements has been much discussed but is little understood. It is not ...
... viruses move between individuals, at least some transposable elements can move between genomes (between individuals) as well as within an individual’s genome. Given their prevalence in genomes, the function (if any) of transposable elements has been much discussed but is little understood. It is not ...
Phylogenetic Network and Physicochemical Properties of
... physicochemical properties relevant to protein evolution (Xia and Li 1998), namely polarity, size, isoelectric point, aliphatic and aromatic nature, and hydropathy. We defined amino acids with polarity 8.6 (Grantham 1974) as polar, amino acids with a side chain molecular volume 61Å3 (Grantham 197 ...
... physicochemical properties relevant to protein evolution (Xia and Li 1998), namely polarity, size, isoelectric point, aliphatic and aromatic nature, and hydropathy. We defined amino acids with polarity 8.6 (Grantham 1974) as polar, amino acids with a side chain molecular volume 61Å3 (Grantham 197 ...
Optimization and the Robustness of BOX A1R PCR for DNA
... fecal source (16). Repetitive element sequence-based PCR (rep-PCR) specifically amplifies repetitive portions of the bacterial genome (7, 11). The resulting mixture of amplified DNA fragments is resolved in gels, producing a banding profile referred to as a rep-PCR genomic DNA fingerprint (7,11). Re ...
... fecal source (16). Repetitive element sequence-based PCR (rep-PCR) specifically amplifies repetitive portions of the bacterial genome (7, 11). The resulting mixture of amplified DNA fragments is resolved in gels, producing a banding profile referred to as a rep-PCR genomic DNA fingerprint (7,11). Re ...
Laboratory manual for the diagnosis of whooping cough caused by... pertussis/ Bordetella parapertussis
... Culture is not very sensitive since the percentage of success is generally not higher than 60%. The highest rates are obtained with infants. It is important to continue to culture in order to analyze the evolution and adaptation of the pathogen and perform surveillance of the antibiotic resistance. ...
... Culture is not very sensitive since the percentage of success is generally not higher than 60%. The highest rates are obtained with infants. It is important to continue to culture in order to analyze the evolution and adaptation of the pathogen and perform surveillance of the antibiotic resistance. ...
XRCC1 interacts with the p58 subunit of DNA Pol a
... mass spectrometry. Sypro ruby stained gel after GST pull-down of HeLa cell extracts expressing either GST or GST-hXRCC1-BRCT1 fused proteins. (B) Identification of XRCC1 associated p58 by immunoprecipitation of XRCC1 from extracts of HeLa cells treated or not with aphidicolin (A: 5 mg/ml, 16 h) and w ...
... mass spectrometry. Sypro ruby stained gel after GST pull-down of HeLa cell extracts expressing either GST or GST-hXRCC1-BRCT1 fused proteins. (B) Identification of XRCC1 associated p58 by immunoprecipitation of XRCC1 from extracts of HeLa cells treated or not with aphidicolin (A: 5 mg/ml, 16 h) and w ...
What`s new - JSI medical systems
... The entry in Suffix can have up to six characters. The suffix is added to the Name of the ROI, e.g: Suffix ng (for NimbleGene), ROI name is BRCA1E01-ng. Therefore all ROIs added on tab Enrichment have a suffix in the ROI name listed in column Name. There is the setting auto cut available: If you use ...
... The entry in Suffix can have up to six characters. The suffix is added to the Name of the ROI, e.g: Suffix ng (for NimbleGene), ROI name is BRCA1E01-ng. Therefore all ROIs added on tab Enrichment have a suffix in the ROI name listed in column Name. There is the setting auto cut available: If you use ...
Analysis of 16S ribosomal RNA gene segments for the diagnosis of
... These nine hyper-variable regions are flanked by conserved stretches in most bacteria, enabling PCR amplification of target sequences using universal primers (Baker et al., 2003; Munson et al., 2004). Identification of bacteria based on the sequences of their 16S-rRNA gene process carried out by ext ...
... These nine hyper-variable regions are flanked by conserved stretches in most bacteria, enabling PCR amplification of target sequences using universal primers (Baker et al., 2003; Munson et al., 2004). Identification of bacteria based on the sequences of their 16S-rRNA gene process carried out by ext ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... sample sequence while the subsequence ‘ACGCAT’ listed between 7 and 12 is the 7th to 12th bases of the sample sequence. The vertical line located between two bases indicates that the upper base is identical to the lower base. By comparing the first subsequence (1st to 6th bases) and the second subse ...
... sample sequence while the subsequence ‘ACGCAT’ listed between 7 and 12 is the 7th to 12th bases of the sample sequence. The vertical line located between two bases indicates that the upper base is identical to the lower base. By comparing the first subsequence (1st to 6th bases) and the second subse ...
DNA-Based Methods for the Identification of Commercial Fish and
... existence of noncoding regions (Lockley and Bardsley 2000). Whereas proteins vary with tissue type, age, and status, DNA is largely independent of these factors and is present in all cell types (Bossier 1999; Civera 2003). Since analytical methods based on DNA have been shown to have several advanta ...
... existence of noncoding regions (Lockley and Bardsley 2000). Whereas proteins vary with tissue type, age, and status, DNA is largely independent of these factors and is present in all cell types (Bossier 1999; Civera 2003). Since analytical methods based on DNA have been shown to have several advanta ...
DNA, RNA, and Protein Synthesis
... During step 2 , enzymes called DNA polymerases add complementary nucleotides, found floating freely inside the nucleus, to each of the original strands. As the nucleotides on the newly forming strand are added, covalent bonds form between the adjacent nucleotides. Covalent bonds form between the deo ...
... During step 2 , enzymes called DNA polymerases add complementary nucleotides, found floating freely inside the nucleus, to each of the original strands. As the nucleotides on the newly forming strand are added, covalent bonds form between the adjacent nucleotides. Covalent bonds form between the deo ...
DNA MUTATION, REPAIR, AND TRANSPOSITION
... Therefore, DNA molecule I is the least sensitive, while molecule III is the most sensitive. 24. Frameshift mutations are caused by insertions or deletions of bases (that are not multiples of 3). These will shift the reading frame for all codons downstream from the mutation. Single base-substitutions ...
... Therefore, DNA molecule I is the least sensitive, while molecule III is the most sensitive. 24. Frameshift mutations are caused by insertions or deletions of bases (that are not multiples of 3). These will shift the reading frame for all codons downstream from the mutation. Single base-substitutions ...
Identification and characterisation of Bacillus subtilis as cellulase
... the presence of genes encoding cellulase CelL15 and CelL73 respectively. The presence of the expected lengths of nucleotide bands at 1,500 bp and 730 bp respectively, indicated the presence of the putative cellulase genes in these isolates. The genes encoding the cellulases CelL15 and CelL73 were cl ...
... the presence of genes encoding cellulase CelL15 and CelL73 respectively. The presence of the expected lengths of nucleotide bands at 1,500 bp and 730 bp respectively, indicated the presence of the putative cellulase genes in these isolates. The genes encoding the cellulases CelL15 and CelL73 were cl ...
A New Sequencing Primer and Workflow Increase 5` Resolution and
... The BigDye® Direct Cycle Sequencing Kit improves the PCR sequencing CE workflow relative to both BigDye® Terminator v3.1 POP-7™ polymer and BigDye® Terminator v1.1 POP-6™ polymer, increasing throughput more than 40%. BigDye® Direct combines the advantages of the speed of POP-7™ polymer electrophores ...
... The BigDye® Direct Cycle Sequencing Kit improves the PCR sequencing CE workflow relative to both BigDye® Terminator v3.1 POP-7™ polymer and BigDye® Terminator v1.1 POP-6™ polymer, increasing throughput more than 40%. BigDye® Direct combines the advantages of the speed of POP-7™ polymer electrophores ...
Canadian Journal of Microbiology
... culturable status (Tholozan et al. 1999), culture-dependent methods do not accurately reflect the actual bacterial community structure but rather the selectivity of growth media for certain bacteria. However, only a few studies have been published yet that report the application of 16S rDNA based co ...
... culturable status (Tholozan et al. 1999), culture-dependent methods do not accurately reflect the actual bacterial community structure but rather the selectivity of growth media for certain bacteria. However, only a few studies have been published yet that report the application of 16S rDNA based co ...
Q 4 - Good Laboratory Practice when Performing Molecular
... results. False positive results can result from sample-to-sample contamination and, perhaps more commonly, from the carry-over of DNA from a previous amplification of the same target. Other sources of contamination may include cloned DNA and virus cell cultures. Careful consideration should be given ...
... results. False positive results can result from sample-to-sample contamination and, perhaps more commonly, from the carry-over of DNA from a previous amplification of the same target. Other sources of contamination may include cloned DNA and virus cell cultures. Careful consideration should be given ...
TILLING. Traditional Mutagenesis Meets
... Dissemination of TILLING technology to benefit plant research has been a major goal of our NSFfunded project. The process is sufficiently complex, both technically and logistically, that we decided to hold two-day workshops so that potential TILLING providers in the academic community can observe th ...
... Dissemination of TILLING technology to benefit plant research has been a major goal of our NSFfunded project. The process is sufficiently complex, both technically and logistically, that we decided to hold two-day workshops so that potential TILLING providers in the academic community can observe th ...
The effects of teaching style on student learning of DNA
... same topics were covered in both courses, just in different ways. The assessment used as a pretest and posttest was a modification of the National Association of Biology Teachers (NABT) Content Biology test. Both groups were given the pre-test, participated and completed assignments in the classes ...
... same topics were covered in both courses, just in different ways. The assessment used as a pretest and posttest was a modification of the National Association of Biology Teachers (NABT) Content Biology test. Both groups were given the pre-test, participated and completed assignments in the classes ...
Epigenetic: A new approach to etiology of infertility
... modification that occurs in spermatogenesis could modify the accessibility of DNA to transcription factors. The histone methyltransferase (HMT) and histone demethylase (HDM) enzymes regulate the methylation patterns in histones. Methylation at H3K4 is generally associated with gene expression. In a ...
... modification that occurs in spermatogenesis could modify the accessibility of DNA to transcription factors. The histone methyltransferase (HMT) and histone demethylase (HDM) enzymes regulate the methylation patterns in histones. Methylation at H3K4 is generally associated with gene expression. In a ...
The Development of a Genetic Marker for Resistance to Fescue
... Ipswich, MA). The enzyme cuts the DNA sequence at the specific polymorphism if it contains an A; if the sequence contains a G it would not be cut. A gel was then run to allow the DNA fragments to separate so that the genotype of the animals could be determined. The genotypes were then compared with ...
... Ipswich, MA). The enzyme cuts the DNA sequence at the specific polymorphism if it contains an A; if the sequence contains a G it would not be cut. A gel was then run to allow the DNA fragments to separate so that the genotype of the animals could be determined. The genotypes were then compared with ...
Identification and characterization of CHO endogenous gene
... certain stable cell lines resulting in a considerable heterogeneity within the cell population. These undesired effects might be avoided by using CHO endogenous gene regulatory elements. Modern approaches for genome-wide, high-throughput recognition of gene regulatory elements rely on whole genome s ...
... certain stable cell lines resulting in a considerable heterogeneity within the cell population. These undesired effects might be avoided by using CHO endogenous gene regulatory elements. Modern approaches for genome-wide, high-throughput recognition of gene regulatory elements rely on whole genome s ...
Evolutionary significance of stress- induced mutagenesis in
... response to stress and those in which they do not. Studies belonging to the first class of experiments are not the main focus of this paper, as the molecular mechanisms that lead to a preferential generation of adaptive mutations in the target gene are very specific to the system used and it is ther ...
... response to stress and those in which they do not. Studies belonging to the first class of experiments are not the main focus of this paper, as the molecular mechanisms that lead to a preferential generation of adaptive mutations in the target gene are very specific to the system used and it is ther ...
Mutation - FSU Biology
... How to Study Mutation Classification of the types of mutations (Tables 3.1 and 3.2) makes the study of mutation sound altogether straightforward. It is important to realize that our ability to study these different classes of mutations varies widely depending on their rarity and the nature of their ...
... How to Study Mutation Classification of the types of mutations (Tables 3.1 and 3.2) makes the study of mutation sound altogether straightforward. It is important to realize that our ability to study these different classes of mutations varies widely depending on their rarity and the nature of their ...
Microsatellite
![](https://commons.wikimedia.org/wiki/Special:FilePath/STR-Slippage_Dr.Peter_Forster.jpg?width=300)
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.