Chapter 16: THE MOLECULAR BASIS OF INHERITANCE (DNA
... transferred some of the cells to light nitrogen (14N, the ordinary one) so that the newly synthesized strands would be light. They isolated DNA from bacterial cells after one and two generations and centrifuged it to separate DNA into bands based on density. After one division in light nitrogen, DNA ...
... transferred some of the cells to light nitrogen (14N, the ordinary one) so that the newly synthesized strands would be light. They isolated DNA from bacterial cells after one and two generations and centrifuged it to separate DNA into bands based on density. After one division in light nitrogen, DNA ...
Resuspension and storage - Integrated DNA Technologies
... oligonucleotide will therefore correspond to 31 g, or 5 nmoles, of DNA. Dissolving 500 g of DNA (16 OD units) in 500 l of TE will yield a stock primer concentration of 1 g/L, or about a 160 M solution. This converts to 160 picomoles of oligonucleotide per L. An oligonucleotide can be stored a ...
... oligonucleotide will therefore correspond to 31 g, or 5 nmoles, of DNA. Dissolving 500 g of DNA (16 OD units) in 500 l of TE will yield a stock primer concentration of 1 g/L, or about a 160 M solution. This converts to 160 picomoles of oligonucleotide per L. An oligonucleotide can be stored a ...
Infectivity analysis of two variable DNA B components of Mungbean
... (pGA1⋅9B22) (Jacob et al 2003) and KA27 (pGA1⋅5B27) DNA B components of MYMV-Vig, each containing two origins of replication as direct repeats, were constructed in a modified version of the binary vector pGA472 (An et al 1985). The partial dimer clone pGA1⋅5B27 was constructed in this study by cloni ...
... (pGA1⋅9B22) (Jacob et al 2003) and KA27 (pGA1⋅5B27) DNA B components of MYMV-Vig, each containing two origins of replication as direct repeats, were constructed in a modified version of the binary vector pGA472 (An et al 1985). The partial dimer clone pGA1⋅5B27 was constructed in this study by cloni ...
annotation and analysis of newly discovered mycobacteriophage
... identified up to date, including many, as yet, genomic sequences. The current mycobacteriophage genomes have been grouped into clusters using the a program called Splitstree, which compares gene phamilies found in phages as well as possible alternative phylogenetic relationships between them.1 Ther ...
... identified up to date, including many, as yet, genomic sequences. The current mycobacteriophage genomes have been grouped into clusters using the a program called Splitstree, which compares gene phamilies found in phages as well as possible alternative phylogenetic relationships between them.1 Ther ...
Restriction Enzymes in Microbiology, Biotechnology and
... (middle row, middle; eop=1) because the phage DNA carries the protective, PstIspecific, modification. It grows poorly, however, on the PstII R-M system, because this modification does not protect the viral DNA from restriction by PstII (middle row, right plate; eop=1O-6). Conversely, the phage in th ...
... (middle row, middle; eop=1) because the phage DNA carries the protective, PstIspecific, modification. It grows poorly, however, on the PstII R-M system, because this modification does not protect the viral DNA from restriction by PstII (middle row, right plate; eop=1O-6). Conversely, the phage in th ...
Neandertal DNA Sequences and the Origin of Modern Humans
... from 0.4 g of the bone. When the primers L16,209 and H16,271 were used in an amplification from this extract and the product cloned (Figure 2, extract B), ten clones carried the eight differences from the reference sequence observed in the amplifications from the first extract, as well as two change ...
... from 0.4 g of the bone. When the primers L16,209 and H16,271 were used in an amplification from this extract and the product cloned (Figure 2, extract B), ten clones carried the eight differences from the reference sequence observed in the amplifications from the first extract, as well as two change ...
Dowload the key paper evaluation in PDF format
... reported mutations are detected. For example, WE-ARMS is designed to specifically recognize only KRAS G13D change for codon 13 alterations; therefore, missing other point mutations involving codon 13 (e.g., G13C), which cumulatively represent approximately 10% of alterations occurring in this codon. ...
... reported mutations are detected. For example, WE-ARMS is designed to specifically recognize only KRAS G13D change for codon 13 alterations; therefore, missing other point mutations involving codon 13 (e.g., G13C), which cumulatively represent approximately 10% of alterations occurring in this codon. ...
Sequences of Primate Insulin Genes Support
... Sequences of Chimpanzee and African Green Monkey Insulin Genes The human insulin gene hybridizes to a single EcoRI fragment in many primate species whose size is about 12- 13 kb ( S. Seino and G. I. Bell, unpublished observations). EcoRI fragments containing the chimpanzee and African green monkey i ...
... Sequences of Chimpanzee and African Green Monkey Insulin Genes The human insulin gene hybridizes to a single EcoRI fragment in many primate species whose size is about 12- 13 kb ( S. Seino and G. I. Bell, unpublished observations). EcoRI fragments containing the chimpanzee and African green monkey i ...
Real time PCR and it`s role in diagnosis
... The main task in The Forensic Medicine is to investigate deaths from unnatural causes. Forensic science has embraced the use of DNA molecular biology tools for diagnostic purposes more than any other scientific field. The process of routine forensic human identification involves sensitive PCR and ca ...
... The main task in The Forensic Medicine is to investigate deaths from unnatural causes. Forensic science has embraced the use of DNA molecular biology tools for diagnostic purposes more than any other scientific field. The process of routine forensic human identification involves sensitive PCR and ca ...
Identification of the fifth subunit os Saccharomyces cerevisiae
... its similarity to other RFC genes was noted (23). We renamed this gene RFC5 as three other small subunit genes, RFC2-RFC4, and most likely also gene RFC! (CDC44), encoding the large subunit of RF-C, had already been isolated (18,24-27). The RFC5 gene has an open reading frame of 354 codons, with the ...
... its similarity to other RFC genes was noted (23). We renamed this gene RFC5 as three other small subunit genes, RFC2-RFC4, and most likely also gene RFC! (CDC44), encoding the large subunit of RF-C, had already been isolated (18,24-27). The RFC5 gene has an open reading frame of 354 codons, with the ...
Biology 343 Lab (Dorn, Shade)
... OBJECTIVES: Welcome to the laboratory portion of Biology 343. This course is designed to give you hands-on experience with modern genetics techniques. It is not designed to coincide very much with your lecture material; in fact, it could be a course of its own, which is what may happen to it someday ...
... OBJECTIVES: Welcome to the laboratory portion of Biology 343. This course is designed to give you hands-on experience with modern genetics techniques. It is not designed to coincide very much with your lecture material; in fact, it could be a course of its own, which is what may happen to it someday ...
The Function of the Hereditary Materials: Biological
... interactions: as do proteins, RNA molenucleotide sequences that are related to cules often fold up in highly specific ways. the one found in Tetrahymena have been Most of what we know about RNA folding discovered in other types of cells, including has been derived from structural studies of fungi an ...
... interactions: as do proteins, RNA molenucleotide sequences that are related to cules often fold up in highly specific ways. the one found in Tetrahymena have been Most of what we know about RNA folding discovered in other types of cells, including has been derived from structural studies of fungi an ...
High background radiation areas of Ramsar in Iran
... Health effects of low dose ionizing radiation are the subject of long-term debate and a problem of public concern. To elucidate these effects, the epidemiological works and radiobiological studies are needed. The great interest expressed worldwide for the study of naturally occurring radiation and e ...
... Health effects of low dose ionizing radiation are the subject of long-term debate and a problem of public concern. To elucidate these effects, the epidemiological works and radiobiological studies are needed. The great interest expressed worldwide for the study of naturally occurring radiation and e ...
Forensic Science – Optional Module
... It wasn't until October 16, 1953, that seventeen people interested in developing the field met in Ottawa to form the Canadian Society of Forensic Science. These people were led by RCMP firearms identification expert Inspector James Churchman, and chemist Dr. Charles Farmilo with the Department of Na ...
... It wasn't until October 16, 1953, that seventeen people interested in developing the field met in Ottawa to form the Canadian Society of Forensic Science. These people were led by RCMP firearms identification expert Inspector James Churchman, and chemist Dr. Charles Farmilo with the Department of Na ...
Developing expressed sequence tag libraries and
... (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. ...
... (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. ...
Physical and Topological Properties of Circular DNA
... because of the topological barrier to unwinding. For the same basic reason these DNA's are thermodynamically more stable than the strand separable DNA's in thermal and alkaline melting experiments. The introduction of one single strand scission has a profound effect on the properties of closed circu ...
... because of the topological barrier to unwinding. For the same basic reason these DNA's are thermodynamically more stable than the strand separable DNA's in thermal and alkaline melting experiments. The introduction of one single strand scission has a profound effect on the properties of closed circu ...
The rapidly evolving field of plant centromeres
... Cytological and sequence analyses of centromeres from different Arabidopsis ecotypes have illustrated their dynamic nature; significant large-scale changes that do not disrupt centromere function have occurred over timescales of just a few million years. For examples, several tandem copies of the mi ...
... Cytological and sequence analyses of centromeres from different Arabidopsis ecotypes have illustrated their dynamic nature; significant large-scale changes that do not disrupt centromere function have occurred over timescales of just a few million years. For examples, several tandem copies of the mi ...
A comparison of methods for haplotype inference
... Multiallelic markers are the most informative ones because they present more than two different alleles segregating in the population. The most commonly used multiallelic markers are microsatellites. Microsatellites consist of the repetition of a small DNA sequence, the polymorphism residing in the ...
... Multiallelic markers are the most informative ones because they present more than two different alleles segregating in the population. The most commonly used multiallelic markers are microsatellites. Microsatellites consist of the repetition of a small DNA sequence, the polymorphism residing in the ...
Different Effects of PCR Inhibitors on Multiplex STR Assays
... which co-extract with the perpetrator’s DNA and prevent PCR amplification. Textile dyes, leather and wood from interior crime scenes can also contain inhibitors that interfere with the DNA polymerase’s activity. The impact of these contaminants on the multiplex STR assays can vary from attenuation t ...
... which co-extract with the perpetrator’s DNA and prevent PCR amplification. Textile dyes, leather and wood from interior crime scenes can also contain inhibitors that interfere with the DNA polymerase’s activity. The impact of these contaminants on the multiplex STR assays can vary from attenuation t ...
A Sequential Strand-Displacement Strategy Enables Efficient Six
... NA-templated synthesis (DTS), the use of DNA hybridization to dramatically increase the effective molarity of reactants linked to oligonucleotides, is a powerful strategy to control chemical reactivity in a DNA sequence-programmed manner.14 Because the reaction products of DTS are encoded by the seq ...
... NA-templated synthesis (DTS), the use of DNA hybridization to dramatically increase the effective molarity of reactants linked to oligonucleotides, is a powerful strategy to control chemical reactivity in a DNA sequence-programmed manner.14 Because the reaction products of DTS are encoded by the seq ...
Dynamics and control of DNA sequence amplification
... cycle to achieve mutant enrichment. Still another example arises in the problem of multiplex PCR, where annealing temperatures must be chosen such that several primers can simultaneously anneal, while avoiding the formation of mismatched hybrids. Over the past two decades, many other variants of DNA ...
... cycle to achieve mutant enrichment. Still another example arises in the problem of multiplex PCR, where annealing temperatures must be chosen such that several primers can simultaneously anneal, while avoiding the formation of mismatched hybrids. Over the past two decades, many other variants of DNA ...
Magnusiomyces capitatus (de Hoog et al.) de Hoog et Smith
... lists the media formulation that has been found to be effective for this strain. While other, unspecified media may also produce satisfactory results, a change in media or the absence of an additive from the ATCC recommended media may affect recovery, growth and/or function of this strain. If an alt ...
... lists the media formulation that has been found to be effective for this strain. While other, unspecified media may also produce satisfactory results, a change in media or the absence of an additive from the ATCC recommended media may affect recovery, growth and/or function of this strain. If an alt ...
Application of rpoB sequence similarity analysis, REP‐PCR and
... and Bacillus type strains and isolates, similarity values among 90% and 100% were retrieved, in agreement with Zeigler (2005) who confirmed 16S rRNA gene sequences similarity of Geobacillus type strains, higher than 98Æ5%. Despite the general use of the 16S rRNA gene as framework for modern bacteria ...
... and Bacillus type strains and isolates, similarity values among 90% and 100% were retrieved, in agreement with Zeigler (2005) who confirmed 16S rRNA gene sequences similarity of Geobacillus type strains, higher than 98Æ5%. Despite the general use of the 16S rRNA gene as framework for modern bacteria ...
Acanthamoeba mitochondrial 16S rDNA sequences: inferred
... with the large majority of AK cases. This genotype encompasses at least five species that have been differentiated largely on the basis of morphology. However, because it is generally agreed that morphology alone is unreliable for classification of this organism, there is a need for other markers th ...
... with the large majority of AK cases. This genotype encompasses at least five species that have been differentiated largely on the basis of morphology. However, because it is generally agreed that morphology alone is unreliable for classification of this organism, there is a need for other markers th ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.