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Ch19EukaryoticGeneControl - Environmental
Ch19EukaryoticGeneControl - Environmental

... Repetitive DNA Repetitive DNA & other non-coding sequences account for most of eukaryotic DNA ...
Answers to End-of-Chapter Questions – Brooker et al ARIS site
Answers to End-of-Chapter Questions – Brooker et al ARIS site

... 2. Explain how a frameshift mutation can lead to premature termination of a polypeptide. Answer: Because an insertion or a deletion causes a frameshift, the codons following the frameshift are read in different groupings than in the original gene. Because 3 of the 64 codons code for stop codons, the ...
6 Possible Alleles
6 Possible Alleles

... the world (with the exception of identical twins), living or dead. •Probability of a random match when all 13 loci typed: ~1 in 3 ...
DNA REPLICATION
DNA REPLICATION

... copy – all at the same time keeping track of everything and not mixing up pages ...
Replication Transcription Translation
Replication Transcription Translation

... • Transcription is different from DNA replication – during the process of transcription, only a segment of DNA, or gene, is transferred into an RNA message (whereas replication copies the ...
Biology 20 DNA Replication What do the initials DNA stand for
Biology 20 DNA Replication What do the initials DNA stand for

... Enzymes involved in DNA replication: ...
Deoxyribonucleic Acid
Deoxyribonucleic Acid

... mixture and injected those bacteria with protein digesting enzymes. • 2. He took the same mixture and injected those bacteria with nucleic acid digesting enzymes. • He assumed that the one that was digested and did not infect would be the genetic material. ...
DNA vs. RNA - Chavis Biology
DNA vs. RNA - Chavis Biology

... Compare DNA and RNA. Recognize the chemical names of the DNA and RNA molecules. Identify the three parts of a nucleotide. Interpret an illustration of a nucleotide. Interpret an illustration of a DNA and a RNA ...
Gel Electrophoresis of DNA
Gel Electrophoresis of DNA

... separation of charged particles located in a gel when an electric current is applied • Charged particles can include DNA, amino acids, peptides, etc ...
pp Multiple Choice Identify the letter of the choice that best
pp Multiple Choice Identify the letter of the choice that best

... Separation of DNA fragments by gel electrophoresis ____. a. requires priming b. is controlled by the size of the fragment c. is based on the positive charges of phosphate groups d. is difficult to accomplish e. needs a detergent to denature proteins Sanger's method for determining the nucleotide seq ...
Document
Document

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Comparative Genomic Hybridization (CGH) - CS
Comparative Genomic Hybridization (CGH) - CS

... false positives is α/2 in each tail of distribution. For tumor data: for each gene i find window size k(i) giving highest positive and negative average - val(i). Mark as significant all values > Cup or < Clow. FDR rate is nα/s (for each sample α was chosen so that FDR was closest to 0.01). ...
Chapter 13 DNA - Pearson Places
Chapter 13 DNA - Pearson Places

... The number of times a base sequences repeats varies between individuals. The number of repeating base sequences at ten locations across various chromosome pairs is considered sufficiently accurate to identify an individual. ...
Colony PCR from Yeast or Bacteria
Colony PCR from Yeast or Bacteria

... Add a swipe of a bacterial colony to 50 uL of water in a 200 uL PCR tube. Be careful to get a single colony/patch (do not contaminate with another colony/patch or with agar off the plate). Heat at 98°C for 5 minutes in the PCR machine. STEP 2: REDtaq PCR In this step you will amplify your gene of in ...
Construct DNA Activity
Construct DNA Activity

... material that is found in all cells. DNA contains the “master code” that instructs all cells in their daily jobs. Genes are short pieces of DNA that make up our chromosomes. Each piece, or strand, of DNA that is related to a gene makes up one trait. DNA looks like a twisted “ladder” made of chemical ...
Table S1: Description of the cohort used for the novel - HAL
Table S1: Description of the cohort used for the novel - HAL

... http://blast.ncbi.nlm.nih.gov/Blast.cgi). We first aligned the AB208025 mRNA (containing exons 8 and 9) and the nucleotide collection (nr/nt) database. A perfect sequence match was observed with the AC234421.2 sequence from GeneBank (Homo sapiens FOSMID clone ABC9-43854600O13). Intronic sequences (T ...
Gel Electrophoresis – Virtual Lab
Gel Electrophoresis – Virtual Lab

... In newspapers, on television, and in movies, you often hear about DNA evidence being used to solve crimes. Scientists use a method called gel electrophoresis to separate DNA molecules on the basis of size. Unless you have an identical twin, your complete set of DNA, or your genome, is unique. This v ...
DNA and Replication
DNA and Replication

... (6) Science concepts. The student knows the mechanisms of genetics, including the role of nucleic acids and the principles of Mendelian Genetics. The student is expected to: (A) identify components of DNA, and describe how information for specifying the traits of an organism is carried in the DNA; ( ...
This would be given at the end of the unit
This would be given at the end of the unit

... c. work only on bacterial DNA. d. always break the DNA between guanine and adenine. 15. The term EcoRI refers to a a. restriction enzyme. b. bacterial gene. ...
DNA, RNA, Proteins Review
DNA, RNA, Proteins Review

... D. DNA and proteins DNA replication results in two DNA molecules, ___________________________________________ A. each with two new strands B. one with two new strands and one with 2 original strands C. each with two original strands D. each with one new strand and one original strand Which type(s) o ...
Analyzing DNA Sequence Similarity on the Computer
Analyzing DNA Sequence Similarity on the Computer

... Figure 1: Shared sequences between species 2 and 3 (in green), species 5 and 6 (in purple), or species 7 and 8 (in blue) indicate that these pairs of species are likely to be very closely related. Shared residues highlighted in red indicate that Species 5-8 may have shared a common ancestor at one p ...
Ethanol precipitation of DNA with salts
Ethanol precipitation of DNA with salts

... interested in forming ion pairs between the polyanion (DNA) and the cation (Na+, Mg++, etc). In dilute aqueous solution, DNA and counterions like Na+ and Mg++ are more or less in the free ion form rather than the ion pair form (that is, each ion is surrounded by one or more layers of water molecules ...
Key
Key

... 17. Explain the difference between inherited and environmental traits. Inherited traits are passed from parent to offspring and are a part of an organism’s genetic makeup. Environmental traits are a result of an organism’s environment. 18. Give two examples of inherited traits. Skin color, height, t ...
Reading GuideDNAto protein(CH7)
Reading GuideDNAto protein(CH7)

... So what exactly is the chemical composition of DNA? It is composed of a phosphate-sugar backbone, the sugar and phosphate are covalently bonded in alternating subunits. The sugar found in the structure of DNA is deoxyribose and it is here that the nitrogenous bases bind. In DNA, these nitrogenous ba ...
Primer Design
Primer Design

... In the later rounds most of the DNA is target gene only, and includes the previous primer sequences. Remember that primers are incorporated into the amplified genes! ...
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Microsatellite



A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.
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