A stage-scanning laser confocal microscope and protocol for DNA
... Therefore determination of methylation status of target gene is becoming increasingly important in diagnosis and treatment. Most laboratories use high performance liquid chromatography (HPLC) for the detection of 5methylcytosine residues [7]. However, HPLC requires large amount of DNA and significan ...
... Therefore determination of methylation status of target gene is becoming increasingly important in diagnosis and treatment. Most laboratories use high performance liquid chromatography (HPLC) for the detection of 5methylcytosine residues [7]. However, HPLC requires large amount of DNA and significan ...
All-In-One Precast Agarose Gel Electrophoresis Kit (2x9
... gel (such as 0.8%) that is easy to break. ...
... gel (such as 0.8%) that is easy to break. ...
Identification of Binding Mechanisms in Single Molecule–DNA
... molecules YO and YOYO (Glazer and Rye, 1992). YO also has an extended aromatic system which enables the compound to intercalate into the double helix. YOYO is a bridged YO-dimer and a bis-intercalant: when sliding into the base sequence, the two ring systems enclose two base pairs. It has been propo ...
... molecules YO and YOYO (Glazer and Rye, 1992). YO also has an extended aromatic system which enables the compound to intercalate into the double helix. YOYO is a bridged YO-dimer and a bis-intercalant: when sliding into the base sequence, the two ring systems enclose two base pairs. It has been propo ...
An Unusual Sugar Conformation in the Structure of an RNA/DNA
... and two base-pairs 30 to the PPT. Bold arrows indicate the position where RNase H cuts to remove the PPT. Grey arrow indicates start of “U- or T-box”. (B) The sequence of the RNA/DNA decamer analyzed in this report, where the first adenine is replaced by a cytosine to improve crystal packing. Number ...
... and two base-pairs 30 to the PPT. Bold arrows indicate the position where RNase H cuts to remove the PPT. Grey arrow indicates start of “U- or T-box”. (B) The sequence of the RNA/DNA decamer analyzed in this report, where the first adenine is replaced by a cytosine to improve crystal packing. Number ...
Slide 1
... Watson and Crick reported that DNA consisted of two polynucleotide strands wrapped into a double helix. – The sugar-phosphate backbone is on the outside. – The nitrogenous bases are perpendicular to the backbone in the interior. – Specific pairs of bases give the helix a uniform shape. – A pairs w ...
... Watson and Crick reported that DNA consisted of two polynucleotide strands wrapped into a double helix. – The sugar-phosphate backbone is on the outside. – The nitrogenous bases are perpendicular to the backbone in the interior. – Specific pairs of bases give the helix a uniform shape. – A pairs w ...
English - progeni - Indiana University
... phosphate group, and nitrogen base). From left to right - Mike Pauciulo, Diane Marek, William Nichols, PhD, Veronika There are four different nucleotides Michaels which make up the DNA molecule: adenine, guanine, cytosine, and thymine. Adenine pairs with thymine while cytosine pairs with guanine for ...
... phosphate group, and nitrogen base). From left to right - Mike Pauciulo, Diane Marek, William Nichols, PhD, Veronika There are four different nucleotides Michaels which make up the DNA molecule: adenine, guanine, cytosine, and thymine. Adenine pairs with thymine while cytosine pairs with guanine for ...
atomic structure of the DNA double
... years ago. Researchers have wondered which were the first biological molecules. How could life begin if the DNA molecules of the genetic code can only be reproduced and deciphered with the aid of protein enzymes, and proteins can only be produced by means of genetic information from DNA? Which came ...
... years ago. Researchers have wondered which were the first biological molecules. How could life begin if the DNA molecules of the genetic code can only be reproduced and deciphered with the aid of protein enzymes, and proteins can only be produced by means of genetic information from DNA? Which came ...
We are interested in computational problems motivated by
... forms the backbone of the polypeptide chain" See Figure 7. The carbon in the centre is called the a-carbon, a-C. Specificity is provided by the 20 different kinds of side-chains attached to the a-carbon. Orientation of the polypeptide: As in the backbone of the DNAJRNA, we note that each monomer is ...
... forms the backbone of the polypeptide chain" See Figure 7. The carbon in the centre is called the a-carbon, a-C. Specificity is provided by the 20 different kinds of side-chains attached to the a-carbon. Orientation of the polypeptide: As in the backbone of the DNAJRNA, we note that each monomer is ...
the Note
... A child is born out of wedlock. The mother needs to claim maintenance for the child, but does not know which one of two men is the father. She has had blood tests done, but both the men have the same blood type. Her next alternative is to do DNA fingerprinting. Both men provide samples and the VNTR ...
... A child is born out of wedlock. The mother needs to claim maintenance for the child, but does not know which one of two men is the father. She has had blood tests done, but both the men have the same blood type. Her next alternative is to do DNA fingerprinting. Both men provide samples and the VNTR ...
The Role of NS5A RNA Binding Activity in Hepatitis C Virus
... to verify the presence of the DDD mutation since this mutation removes a Blp1 cut site that is found in wild type DNA. Screening with the Blp1 showed that the DDD was present because the plasmid DNA was not cut by the Blp1 enzyme (Figure 14). Sequencing the DNA showed that the DDD was the only chang ...
... to verify the presence of the DDD mutation since this mutation removes a Blp1 cut site that is found in wild type DNA. Screening with the Blp1 showed that the DDD was present because the plasmid DNA was not cut by the Blp1 enzyme (Figure 14). Sequencing the DNA showed that the DDD was the only chang ...
Sec_12_2 PPT
... explained how DNA carried information and could be copied. Watson and Crick's model of DNA was a double helix, in which two strands were wound around each other. ...
... explained how DNA carried information and could be copied. Watson and Crick's model of DNA was a double helix, in which two strands were wound around each other. ...
Responsibilities of the intern
... Traditional methods of measuring and assessing arthropod community composition and diversity are time consuming, unreliable and require high expertise. Metabarcoding represents a powerful tool for biodiversity conservation and ecosystem management at the organismal to community level because it is m ...
... Traditional methods of measuring and assessing arthropod community composition and diversity are time consuming, unreliable and require high expertise. Metabarcoding represents a powerful tool for biodiversity conservation and ecosystem management at the organismal to community level because it is m ...
Characterization the binding of divalent metal and phosphate ions in
... and a histidine residue (Kleanthous et al. 1999). However, we did not find any Mg2+ binding sites either in apo-enzyme or zinc-holo-enzyme crystals soaked with Mg2+ ions. The original metal-binding site in the H-N-H motif of the apo-enzyme soaked with Mg2+ ions was occupied with water molecules simi ...
... and a histidine residue (Kleanthous et al. 1999). However, we did not find any Mg2+ binding sites either in apo-enzyme or zinc-holo-enzyme crystals soaked with Mg2+ ions. The original metal-binding site in the H-N-H motif of the apo-enzyme soaked with Mg2+ ions was occupied with water molecules simi ...
SCIENCE AS A PROCESS
... Grew bacteria for many generations in radioactive (heavy) 15N . . . so all DNA is heavy Then grow in 14N, centrifuge as generations divide, and check to see where heavy DNA ends up ...
... Grew bacteria for many generations in radioactive (heavy) 15N . . . so all DNA is heavy Then grow in 14N, centrifuge as generations divide, and check to see where heavy DNA ends up ...
How Does Replication-Associated Mutational Pressure Influence
... other and disappear, leaving the effect of asymmetry introduced by other mechanisms (see Methods for details). In contrast, the asymmetry in ORFs resulting from their coding function or transcription is of the same sign independent of their location on leading or lagging strands. Thus, the addition ...
... other and disappear, leaving the effect of asymmetry introduced by other mechanisms (see Methods for details). In contrast, the asymmetry in ORFs resulting from their coding function or transcription is of the same sign independent of their location on leading or lagging strands. Thus, the addition ...
Gel Electrophoresis - Integrated DNA Technologies
... expression is a constant and mobility is completely determined by molecular length. As a matter of practice, it is difficult to accurately resolve double-stranded nucleic acids smaller than about 100 bases in an agarose gel because the sieving properties of agarose are not fine enough. On the other ...
... expression is a constant and mobility is completely determined by molecular length. As a matter of practice, it is difficult to accurately resolve double-stranded nucleic acids smaller than about 100 bases in an agarose gel because the sieving properties of agarose are not fine enough. On the other ...
Biology DNA: The Genetic Material
... It occurs during the synthesis (S) phase of the cell cycle, before a cell divides. The process can be broken down into three steps. Step 1: Before replication can begin, the double helix must unwind. This is accomplished by enzymes called DNA helicases, which open up the double helix by breaking the ...
... It occurs during the synthesis (S) phase of the cell cycle, before a cell divides. The process can be broken down into three steps. Step 1: Before replication can begin, the double helix must unwind. This is accomplished by enzymes called DNA helicases, which open up the double helix by breaking the ...
Strings: Theory, Properties and Applications
... in the diagnosis of viruses and bacteria in host organisms. In particular, probes are used to diagnose the presence of viruses and bacteria in biological samples. A probe is a strand of DNA or RNA opportunely treated with a radioactive isotope, dye, or enzyme, and used to easily detect the presence ...
... in the diagnosis of viruses and bacteria in host organisms. In particular, probes are used to diagnose the presence of viruses and bacteria in biological samples. A probe is a strand of DNA or RNA opportunely treated with a radioactive isotope, dye, or enzyme, and used to easily detect the presence ...
BIO UNIT 7 CHS 9- 10 DNA Replication-Transcription
... The instructions in DNA are in a code that depends on the arrangement of nucleotide bases. The nucleotides of DNA are arranged in triplets, or groups of three. There are 64 possible triplets. The code for making proteins is passed from the DNA to an RNA molecule during transcription. ...
... The instructions in DNA are in a code that depends on the arrangement of nucleotide bases. The nucleotides of DNA are arranged in triplets, or groups of three. There are 64 possible triplets. The code for making proteins is passed from the DNA to an RNA molecule during transcription. ...
GENETIC INFORMATION NONDISCRIMINATION ACT
... “adequate security” to minimize contamination without providing for accountability in the event of contamination. Similarly, §28 provides for audits of DNA laboratories only, withholding from similar scrutiny of the DNA Profiling Board itself. ...
... “adequate security” to minimize contamination without providing for accountability in the event of contamination. Similarly, §28 provides for audits of DNA laboratories only, withholding from similar scrutiny of the DNA Profiling Board itself. ...
Unit VI Structure and Function of DNA/RNA Teaching Module B-4
... nucleotides and base pairs; therefore, the primary focus of assessment should be to detect similarities and differences between structure of DNA and RNA, the nucleotides that compose DNA and RNA, and the bases that bond to form DNA and RNA. In addition to compare, assessments may require students to ...
... nucleotides and base pairs; therefore, the primary focus of assessment should be to detect similarities and differences between structure of DNA and RNA, the nucleotides that compose DNA and RNA, and the bases that bond to form DNA and RNA. In addition to compare, assessments may require students to ...
Robust PCR amplification of GC-rich targets with Hot Start 7
... PCR is a well-known and effective tool for the amplification of DNA targets of interest. When DNA targets high in GC content are amplified, PCR product formation can often be compromised by inadequate strand separation and the propensity for complex secondary structure formation. The inability of th ...
... PCR is a well-known and effective tool for the amplification of DNA targets of interest. When DNA targets high in GC content are amplified, PCR product formation can often be compromised by inadequate strand separation and the propensity for complex secondary structure formation. The inability of th ...
Single-molecule studies of DNA replication Geertsema, Hylkje
... showed that both leading- and lagging-strand synthesis are resistant to dilution confirming the recycling of replication proteins by the T7 replisome (46). In addition, kinetic studies demonstrated that the T4 replisome is highly processive and potentially able to replicate the entire T4 genome (172 ...
... showed that both leading- and lagging-strand synthesis are resistant to dilution confirming the recycling of replication proteins by the T7 replisome (46). In addition, kinetic studies demonstrated that the T4 replisome is highly processive and potentially able to replicate the entire T4 genome (172 ...
DNA nanotechnology
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.