Force spectroscopy of single DNA and RNA molecules Mark C
... force than that observed experimentally [20–22]. Rouzina and Bloomfield [23,24] have proposed an alternative model for DNA overstretching as a force-induced melting process. In this model, the base pairs holding the two DNA strands together break as the DNA unwinds during the transition. This model ...
... force than that observed experimentally [20–22]. Rouzina and Bloomfield [23,24] have proposed an alternative model for DNA overstretching as a force-induced melting process. In this model, the base pairs holding the two DNA strands together break as the DNA unwinds during the transition. This model ...
Diversity of Endonuclease V: From DNA Repair to RNA Editing
... reaction and a nitrosative reaction. The generated deaminated products are potentially mutagenic because of their structural similarity to natural bases, which in turn leads to erroneous nucleotide pairing and subsequent disruption of cellular metabolism. Incorporation of deaminated precursors into ...
... reaction and a nitrosative reaction. The generated deaminated products are potentially mutagenic because of their structural similarity to natural bases, which in turn leads to erroneous nucleotide pairing and subsequent disruption of cellular metabolism. Incorporation of deaminated precursors into ...
Rapid and effective genotyping of Cre transgenic mice
... genome manipulation and has been successfully used to generate mouse mutants (1, 2). Initially, transgenic mouse lines are produced: one expressing the Cre recombinase and one carrying 2 loxP sites (34 bp sequences). Upon crossing Cre and loxP strains, the Cre recombinase cuts specifically at the lo ...
... genome manipulation and has been successfully used to generate mouse mutants (1, 2). Initially, transgenic mouse lines are produced: one expressing the Cre recombinase and one carrying 2 loxP sites (34 bp sequences). Upon crossing Cre and loxP strains, the Cre recombinase cuts specifically at the lo ...
Lecture 6 DNA structure replication DNA structure, replication, and
... Non-crossover occurs when h b both th jjunctions ti are resolved in same plane • Crossover occurs with the two junctions are resolved in different planes ...
... Non-crossover occurs when h b both th jjunctions ti are resolved in same plane • Crossover occurs with the two junctions are resolved in different planes ...
myDNA
... •Archeology: Cloning mummy DNA! •Bio-History: Using DNA to Understand the Past! •The Tell-Tale Heart: DNA Identifies Son of King Louis XVI and Queen Marie Antoinette •Bio-Terrorism and Anthrax •Law, Justice, and The Innocence Project •DNA Privacy and Genetic Discrimination •Lawsuit: Can They Make Yo ...
... •Archeology: Cloning mummy DNA! •Bio-History: Using DNA to Understand the Past! •The Tell-Tale Heart: DNA Identifies Son of King Louis XVI and Queen Marie Antoinette •Bio-Terrorism and Anthrax •Law, Justice, and The Innocence Project •DNA Privacy and Genetic Discrimination •Lawsuit: Can They Make Yo ...
R5C - Royal Society of Chemistry
... Act, 1988, this publication may not be reproduced, stored, or transmitted, in any form or by any means, without the prior permission in writing of the publishers, or in the case of reprographic reproduction, only in accordance with the terms of the licences issued by the Copyright Licensing Agency i ...
... Act, 1988, this publication may not be reproduced, stored, or transmitted, in any form or by any means, without the prior permission in writing of the publishers, or in the case of reprographic reproduction, only in accordance with the terms of the licences issued by the Copyright Licensing Agency i ...
Lesson Overview - Enfield High School
... Prokaryotes = Cytoplasm Eukaryotes = In the cell’s nucleus ...
... Prokaryotes = Cytoplasm Eukaryotes = In the cell’s nucleus ...
Molecular Structure of the GARP Family of Plant Myb
... (Kerstetter et al., 2001). Little is known about the structure and function of plant Myb-related domains, including the B motif (or GARP motif). In the hope of better understanding the features of these widespread plant Myb-related motifs, we have characterized a representative B motif (from ARR10) ...
... (Kerstetter et al., 2001). Little is known about the structure and function of plant Myb-related domains, including the B motif (or GARP motif). In the hope of better understanding the features of these widespread plant Myb-related motifs, we have characterized a representative B motif (from ARR10) ...
11.1 How Did Scientists Discover That Genes Are Made of DNA?
... 11.2 What Is the Structure of DNA? Hydrogen bonds between complementary bases hold two DNA strands together in a double helix (continued) – Adenine and guanine are large molecules; thymine and cytosine are relatively smaller – Because base pairing always places a large molecule with a small one, ...
... 11.2 What Is the Structure of DNA? Hydrogen bonds between complementary bases hold two DNA strands together in a double helix (continued) – Adenine and guanine are large molecules; thymine and cytosine are relatively smaller – Because base pairing always places a large molecule with a small one, ...
DNA Shape Dominates Sequence Affinity in Nucleosome Formation
... strong electrostatic attraction to the positively charged histone surface. Protein-bound sites along DNA present barriers to transcription; thus, their positioning is a crucial element in the regulation of cellular function for all eukaryotic species [1–3]. In spite of being central to biology, the ...
... strong electrostatic attraction to the positively charged histone surface. Protein-bound sites along DNA present barriers to transcription; thus, their positioning is a crucial element in the regulation of cellular function for all eukaryotic species [1–3]. In spite of being central to biology, the ...
Solution - Glencoe
... The mRNA strand shown below is in the process of synthesizing—making—proteins from amino acids. tRNA molecules bring the amino acids to the mRNA strand. Circle the tRNA molecule that will attach to the mRNA strand. Remember, cytosine (C) pairs with guanine (G), and adenine (A) pairs with uracil (U). ...
... The mRNA strand shown below is in the process of synthesizing—making—proteins from amino acids. tRNA molecules bring the amino acids to the mRNA strand. Circle the tRNA molecule that will attach to the mRNA strand. Remember, cytosine (C) pairs with guanine (G), and adenine (A) pairs with uracil (U). ...
A Structural Insight into Major Groove Directed Binding of
... Significant developments have been made over the past few years in the area of drug-DNA interactions using different biophysical methods and spectroscopic techniques [11,12]. Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy has emerged as an efficient tool in providing ...
... Significant developments have been made over the past few years in the area of drug-DNA interactions using different biophysical methods and spectroscopic techniques [11,12]. Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy has emerged as an efficient tool in providing ...
Quantum Well Electron Gain Structures and Infrared
... Some critters (i.e. some viruses) seem to run on RNA-only But … they seem to be dependent on DNA-bearing hosts for survival (??) • At least opens the possibility of “RNA world” life, which then evolved into more complex “DNA-world” we all know and love today ...
... Some critters (i.e. some viruses) seem to run on RNA-only But … they seem to be dependent on DNA-bearing hosts for survival (??) • At least opens the possibility of “RNA world” life, which then evolved into more complex “DNA-world” we all know and love today ...
Functional implications of genetic variation in non
... interactions, but not that these are actually occurring in vivo. The EMSA technique has been applied to the functional characterization of many SNPs. A number of important host genetic determinants of susceptibility to malaria infection have been identified, including possession of the Duffy blood g ...
... interactions, but not that these are actually occurring in vivo. The EMSA technique has been applied to the functional characterization of many SNPs. A number of important host genetic determinants of susceptibility to malaria infection have been identified, including possession of the Duffy blood g ...
Variable effects of DNA-synthesis inhibitors upon DNA methylation
... aphidicolin at the indicated dosages for a period of 24 h in the presence of labelled DNA precursor as described in text. Results are displayed as % of control and are the average of 6-10 determinations. Open bars represent % of cytosine methylation. Closed bars represent rate of DNA synthesis. All ...
... aphidicolin at the indicated dosages for a period of 24 h in the presence of labelled DNA precursor as described in text. Results are displayed as % of control and are the average of 6-10 determinations. Open bars represent % of cytosine methylation. Closed bars represent rate of DNA synthesis. All ...
Lecture 27
... • Enhancers are transcriptional control regions that can be located several thousand base pairs upstream or downstream from the transcription start site. ...
... • Enhancers are transcriptional control regions that can be located several thousand base pairs upstream or downstream from the transcription start site. ...
Extensive protein and DNA backbone sampling
... fragment moves, in which a continuous stretch of base-paired DNA is replaced by a base paired region of equal length taken from a DNA duplex of known structure (Figure 2A); interface fragment moves, in which the orientation of the protein relative to the DNA is updated based on a template interface ...
... fragment moves, in which a continuous stretch of base-paired DNA is replaced by a base paired region of equal length taken from a DNA duplex of known structure (Figure 2A); interface fragment moves, in which the orientation of the protein relative to the DNA is updated based on a template interface ...
Influence of the environment and probes on rapid DNA sequencing
... In their pioneering work Kasianowicz et al. demonstrated that DNA can be pulled through a biological nanopore roughly the size of the DNA itself. [4] The translocation of the DNA can be detected by measuring a blockade current when ions are partially prevented from entering the pore. More recent exp ...
... In their pioneering work Kasianowicz et al. demonstrated that DNA can be pulled through a biological nanopore roughly the size of the DNA itself. [4] The translocation of the DNA can be detected by measuring a blockade current when ions are partially prevented from entering the pore. More recent exp ...
Active-site Determination of a Pyrimidine Dimer
... DNA, reactions were scaled up to allow for sequence analysis of the trapped complex. The rationale behind this experiment was that if the active-site nucleophile were any of the e-NH2 groups in the enzyme, then N-terminal amino acid sequencing of the enzyme within the complex should yield a sequence ...
... DNA, reactions were scaled up to allow for sequence analysis of the trapped complex. The rationale behind this experiment was that if the active-site nucleophile were any of the e-NH2 groups in the enzyme, then N-terminal amino acid sequencing of the enzyme within the complex should yield a sequence ...
Energetics of protein–DNA interactions
... altogether (3–7). Those methods that do consider indirect readout have used two different types of methods: the knowledge-based method of Olson et al. (8) or a molecular mechanics method (9–11). An initial comparison between these two methods of indirect readout has recently been carried out (12). As ...
... altogether (3–7). Those methods that do consider indirect readout have used two different types of methods: the knowledge-based method of Olson et al. (8) or a molecular mechanics method (9–11). An initial comparison between these two methods of indirect readout has recently been carried out (12). As ...
Electroosmotic screening of the DNA charge in a
... 5 ns was required to balance the effective driving force by the force of the spring. Each simulation was continued for an additional 15 ns to determine the radial distribution of ions, the velocity profile of water, and the average stall force F . In a nanopore, the radial density of counterions dep ...
... 5 ns was required to balance the effective driving force by the force of the spring. Each simulation was continued for an additional 15 ns to determine the radial distribution of ions, the velocity profile of water, and the average stall force F . In a nanopore, the radial density of counterions dep ...
University of Groningen DNA Block Copolymers Schnitzler
... functionalization of modified single-stranded DNA by WatsonCrick base-pairing is another key feature of DNA block copolymers. Furthermore, the appropriate selection of DNA sequence and organic polymer gives control over the material properties and their self-assembly into supramolecular structures. ...
... functionalization of modified single-stranded DNA by WatsonCrick base-pairing is another key feature of DNA block copolymers. Furthermore, the appropriate selection of DNA sequence and organic polymer gives control over the material properties and their self-assembly into supramolecular structures. ...
The nucleic acids - faculty at Chemeketa
... • DNA in the nucleus of the cell directs the sythesis of an RNA molecule. – The RNA will carry the sequence of amines found on a particular portion of the DNA • Only a portion of a DNA strand is used to make any given RNA. • There needs to be a way to start and stop transcription. • The DNA has syst ...
... • DNA in the nucleus of the cell directs the sythesis of an RNA molecule. – The RNA will carry the sequence of amines found on a particular portion of the DNA • Only a portion of a DNA strand is used to make any given RNA. • There needs to be a way to start and stop transcription. • The DNA has syst ...
Monitoring endangered freshwater biodiversity using environmental
... Although of great potential for contemporary biodiversity monitoring, environmental DNA detection in wild populations has so far only been applied to a few common or invasive species of amphibians and fish (Ficetola et al. 2008; Goldberg et al. 2011; Jerde et al. 2011). The potential for monitoring ...
... Although of great potential for contemporary biodiversity monitoring, environmental DNA detection in wild populations has so far only been applied to a few common or invasive species of amphibians and fish (Ficetola et al. 2008; Goldberg et al. 2011; Jerde et al. 2011). The potential for monitoring ...
CHAPTER 6
... notice that the specific (base) pairing we have postulated immediately suggests a possible copying mechanism for the genetic material." • The mechanism: Strand separation, followed by copying of each strand. • Each separated strand acts as a template for the synthesis of a new complementary strand. ...
... notice that the specific (base) pairing we have postulated immediately suggests a possible copying mechanism for the genetic material." • The mechanism: Strand separation, followed by copying of each strand. • Each separated strand acts as a template for the synthesis of a new complementary strand. ...
DNA nanotechnology
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.