Unit 5 Molecular Genetics CLASS NOTES
... Helicase – enzyme that breaks H-bonds DNA Polymerase – enzyme that catalyzes connection of nucleotides to form complementary DNA strand in 5’ to 3’ direction (reads template in 3’ to 5’ direction) Leading Strand – transcribed continuously in 5’ to 3’ direction Lagging Strand – transcribed in segment ...
... Helicase – enzyme that breaks H-bonds DNA Polymerase – enzyme that catalyzes connection of nucleotides to form complementary DNA strand in 5’ to 3’ direction (reads template in 3’ to 5’ direction) Leading Strand – transcribed continuously in 5’ to 3’ direction Lagging Strand – transcribed in segment ...
DNA Knex modelling lab (1)
... help of Rosalind Franklin, Maurice Wilkens and Erwin Chargraff. The shape of DNA is a double stranded helix, which is like a twisted ladder. The two strands run antiparallel to one another, or in opposite directions. The sides of the ladder are made of alternating deoxyribose sugar and phosphate mol ...
... help of Rosalind Franklin, Maurice Wilkens and Erwin Chargraff. The shape of DNA is a double stranded helix, which is like a twisted ladder. The two strands run antiparallel to one another, or in opposite directions. The sides of the ladder are made of alternating deoxyribose sugar and phosphate mol ...
Agarose Gel Electrophoresis
... fragment from fragments of similar size); if the goal is to separate small fragment, a high concentration of agarose should be used (e.g., use 2 % agarose if you want to separate a 100-bp fragment from fragments of similar size) - conformation of the DNA: the relationship between rate of migration a ...
... fragment from fragments of similar size); if the goal is to separate small fragment, a high concentration of agarose should be used (e.g., use 2 % agarose if you want to separate a 100-bp fragment from fragments of similar size) - conformation of the DNA: the relationship between rate of migration a ...
Gel Electrophoresis of DNA
... Restriction Enzyme: (An enzyme from bacteria that can recognize specific base sequences in DNA and cut the DNA at that site (the restriction site). A restriction enzyme acts as a biochemical scissors. ...
... Restriction Enzyme: (An enzyme from bacteria that can recognize specific base sequences in DNA and cut the DNA at that site (the restriction site). A restriction enzyme acts as a biochemical scissors. ...
Examination IV Key
... to PCR amplify a region of the X chromosome that includes base pairs 10,491,100 through 10,491,250 but no other DNA sequences from the X chromosome. The nucleotide numbering is from the p arm telomere to the q arm telomere of this chromosome. Given the sequence of nucleotides (for the strand going 5 ...
... to PCR amplify a region of the X chromosome that includes base pairs 10,491,100 through 10,491,250 but no other DNA sequences from the X chromosome. The nucleotide numbering is from the p arm telomere to the q arm telomere of this chromosome. Given the sequence of nucleotides (for the strand going 5 ...
RNA_and_Protein_Synthesis
... a particular amino acid, its anticodons circle around in the cytoplasm and pick up the correct amino acid . It takes the amino acids over to the ribosome where the acids bond together in a long polypetide chain. The tRNA is then released back to the cytoplasm. ...
... a particular amino acid, its anticodons circle around in the cytoplasm and pick up the correct amino acid . It takes the amino acids over to the ribosome where the acids bond together in a long polypetide chain. The tRNA is then released back to the cytoplasm. ...
MCDB 1041: Using DNA To manipulate DNA in the laboratory, one
... The letters E, P, B, and H along the DNA signify specific RE sites: nucleotide sequences where restriction enzymes cut the DNA. For example “E” is where a restriction enzyme called Eco RI cuts. ...
... The letters E, P, B, and H along the DNA signify specific RE sites: nucleotide sequences where restriction enzymes cut the DNA. For example “E” is where a restriction enzyme called Eco RI cuts. ...
molbioDay1
... In the next weeks you will transform E. coli to glow yellow by inserting a plasmid with the gene for YFP (yellow fluorescent protein). To do this, we will perform four steps: prepare the insert by copying the YFP insert by PCR, remove the LacZ gene from the backbone with restriction enzyme digestion ...
... In the next weeks you will transform E. coli to glow yellow by inserting a plasmid with the gene for YFP (yellow fluorescent protein). To do this, we will perform four steps: prepare the insert by copying the YFP insert by PCR, remove the LacZ gene from the backbone with restriction enzyme digestion ...
Transcription 12.06.22A lec
... [23] different chromosomes, each of which has two pairs of DNA that form a helix, the famous double helix pattern. These different bases end up making hydrogen-‐bonding pairs. We norma ...
... [23] different chromosomes, each of which has two pairs of DNA that form a helix, the famous double helix pattern. These different bases end up making hydrogen-‐bonding pairs. We norma ...
DNA Study Guide
... 13. Explain why there are always equal numbers of purines and pyramidines in a DNA strand. 14. Briefly describe Griffith’s contribution to our current understanding of DNA. 15. Briefly describe Hershey and Chase’s contribution to our current understanding of DNA. 16. Explain why Hershey and Chase us ...
... 13. Explain why there are always equal numbers of purines and pyramidines in a DNA strand. 14. Briefly describe Griffith’s contribution to our current understanding of DNA. 15. Briefly describe Hershey and Chase’s contribution to our current understanding of DNA. 16. Explain why Hershey and Chase us ...
Biology_1_&_2_files/6 DNA and RNA ACADEMIC
... complementary strands are made you need several enzymes for replication to occur in prokaryotes, begins at a ...
... complementary strands are made you need several enzymes for replication to occur in prokaryotes, begins at a ...
26.6 Replication of DNA
... • Initiation: Protein synthesis begins when an mRNA, the first tRNA, and the small subunit of a ribosome come together. • The first codon on the end of mRNA, an AUG, acts as a “start” signal for the translation machinery and codes for a methionine carrying tRNA. • Initiation is completed when the l ...
... • Initiation: Protein synthesis begins when an mRNA, the first tRNA, and the small subunit of a ribosome come together. • The first codon on the end of mRNA, an AUG, acts as a “start” signal for the translation machinery and codes for a methionine carrying tRNA. • Initiation is completed when the l ...
Historical Basis of Modern Understanding
... Experiments conducted by Martha Chase and Alfred Hershey in 1952 provided con rmatory evidence that DNA was the genetic material and not proteins. Chase and Hershey were studying a bacteriophage, which is a virus that infects bacteria. Viruses typically have a simple structure: a protein coat, calle ...
... Experiments conducted by Martha Chase and Alfred Hershey in 1952 provided con rmatory evidence that DNA was the genetic material and not proteins. Chase and Hershey were studying a bacteriophage, which is a virus that infects bacteria. Viruses typically have a simple structure: a protein coat, calle ...
Historical Basis of Modern Understanding
... Experiments conducted by Martha Chase and Alfred Hershey in 1952 provided con rmatory evidence that DNA was the genetic material and not proteins. Chase and Hershey were studying a bacteriophage, which is a virus that infects bacteria. Viruses typically have a simple structure: a protein coat, calle ...
... Experiments conducted by Martha Chase and Alfred Hershey in 1952 provided con rmatory evidence that DNA was the genetic material and not proteins. Chase and Hershey were studying a bacteriophage, which is a virus that infects bacteria. Viruses typically have a simple structure: a protein coat, calle ...
DNA pp
... – Steps of replication: The _________ enzyme unzips the double stranded ______. A __________ forms where this occurs. DNA______________, another enzyme, adds the correct _____________ to both of the unwinded strands. It also acts as a _______________...it corrects incorrect nucleotides. There are no ...
... – Steps of replication: The _________ enzyme unzips the double stranded ______. A __________ forms where this occurs. DNA______________, another enzyme, adds the correct _____________ to both of the unwinded strands. It also acts as a _______________...it corrects incorrect nucleotides. There are no ...
Chapter 12 - Fort Bend ISD
... • What are the 3 parts of a DNA molecule – the monomer? • What are the 4 bases in a molecule of DNA? • What type of bond holds two bases together? • What bonds with A? • What bonds with G? • What is the shape of DNA? – What is the backbone made up of? – What are the rungs of the ladder made up of? ...
... • What are the 3 parts of a DNA molecule – the monomer? • What are the 4 bases in a molecule of DNA? • What type of bond holds two bases together? • What bonds with A? • What bonds with G? • What is the shape of DNA? – What is the backbone made up of? – What are the rungs of the ladder made up of? ...
Nucleic Acids: DNA and RNA - E
... RNA is the main genetic material used in the organisms called viruses, and RNA is also important in the production of proteins in other living organisms. RNA can move around the cells of living organisms and thus serves as a sort of genetic messenger, relaying the information stored in the cell's DN ...
... RNA is the main genetic material used in the organisms called viruses, and RNA is also important in the production of proteins in other living organisms. RNA can move around the cells of living organisms and thus serves as a sort of genetic messenger, relaying the information stored in the cell's DN ...
495-Ze15
... replication, namely, strand separation and synthesis of new chains using parent chains as templates. The information containing in DNA molecule is transcribed into base sequence of RNA single chain macromolecule, than (during translation) this information is used for synthesis of protein chains, con ...
... replication, namely, strand separation and synthesis of new chains using parent chains as templates. The information containing in DNA molecule is transcribed into base sequence of RNA single chain macromolecule, than (during translation) this information is used for synthesis of protein chains, con ...
DNA structure and replication notes
... opposite orientation of the strands is important in DNA replication. DNA polymerases link DNA nucleotides to a growing daughter strand, only to the 3’ end of the strand, never to the 5’ end. Thus, a daughter DNA strand can only grow in the 5’3’ direction. ...
... opposite orientation of the strands is important in DNA replication. DNA polymerases link DNA nucleotides to a growing daughter strand, only to the 3’ end of the strand, never to the 5’ end. Thus, a daughter DNA strand can only grow in the 5’3’ direction. ...
Chapter 12 Jeopardy Review
... Replication results in two DNA molecules. Which of the following is true a. ...
... Replication results in two DNA molecules. Which of the following is true a. ...
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... physicist, and neuroscientist. He is known as one of two _____________ of the structure of the DNA molecule. In 1953, basing on X-rayed structure analysis made by Maurice Wilkins, he and James D. Watson _________ the famous DNA double spiral. It was a _________ discovery, because the model explained ...
... physicist, and neuroscientist. He is known as one of two _____________ of the structure of the DNA molecule. In 1953, basing on X-rayed structure analysis made by Maurice Wilkins, he and James D. Watson _________ the famous DNA double spiral. It was a _________ discovery, because the model explained ...
DNA nanotechnology
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.