general introduction
... tightly bound to DNA polymerase α) synthesizes a short RNA primer (8-12 ribonucleotides) after which polymerase α adds another 20 nucleotides. Replication is then continued by the main replicative enzymes (DNA polymerases δ and ε). Since DNA polymerases can only extend DNA at the 3’ end, the leading ...
... tightly bound to DNA polymerase α) synthesizes a short RNA primer (8-12 ribonucleotides) after which polymerase α adds another 20 nucleotides. Replication is then continued by the main replicative enzymes (DNA polymerases δ and ε). Since DNA polymerases can only extend DNA at the 3’ end, the leading ...
Escherichia coli
... contain minimal medium, which provides just the basic nutritional requirements for bacterial growth (nitrogen, carbon and energy sources, plus some salts). The medium on the left is supplemented with tryptophan but the medium on the right is not. Unmutated bacteria, plus tryptophan auxotrophs, can g ...
... contain minimal medium, which provides just the basic nutritional requirements for bacterial growth (nitrogen, carbon and energy sources, plus some salts). The medium on the left is supplemented with tryptophan but the medium on the right is not. Unmutated bacteria, plus tryptophan auxotrophs, can g ...
DNA
... DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification. Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions. Commercial kits are now available for easy PCR reaction setup an ...
... DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification. Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions. Commercial kits are now available for easy PCR reaction setup an ...
DNA Profiling
... the mother and the man. DNA profiles are then prepared and examined. If all the bands in the child’s profile math with the bands in either the mothers or the mans profile then the man is shown to be the natural father of the child. If some of the child’s bands match those of the mother but the rest ...
... the mother and the man. DNA profiles are then prepared and examined. If all the bands in the child’s profile math with the bands in either the mothers or the mans profile then the man is shown to be the natural father of the child. If some of the child’s bands match those of the mother but the rest ...
DNA Questions – mahon – (26)
... The DNA is transcribed into mRNA which reads UACGCA. The anticodon needed to pair with this would be AUGCGU. ...
... The DNA is transcribed into mRNA which reads UACGCA. The anticodon needed to pair with this would be AUGCGU. ...
II. Building a Model of DNA
... are the structural units of proteins. A polypeptide chain is made of many amino acids bonded together. The key to the genetic code is the sequence of nitrogen bases along one side of the DNA molecule. To construct a protein you must know the order of the bases. The code is written in three letter “w ...
... are the structural units of proteins. A polypeptide chain is made of many amino acids bonded together. The key to the genetic code is the sequence of nitrogen bases along one side of the DNA molecule. To construct a protein you must know the order of the bases. The code is written in three letter “w ...
Exam II Review Questions
... d. To purify the DNA from other materials in the extract. e. All of the above. The diagram shows a step in the experiment by Avery, MacCleod and McCarty in which they demonstrated that DNA was the genetic material. Recall that they made an extract from the S strain bacteria and mixed the extract wi ...
... d. To purify the DNA from other materials in the extract. e. All of the above. The diagram shows a step in the experiment by Avery, MacCleod and McCarty in which they demonstrated that DNA was the genetic material. Recall that they made an extract from the S strain bacteria and mixed the extract wi ...
Nerve activates contraction
... • In addition, Watson and Crick determined that chemical side groups off the nitrogen bases would form hydrogen bonds, connecting the two strands. • Based on details of their structure, adenine would form two hydrogen bonds only with thymine and guanine would form three hydrogen bonds only with cyt ...
... • In addition, Watson and Crick determined that chemical side groups off the nitrogen bases would form hydrogen bonds, connecting the two strands. • Based on details of their structure, adenine would form two hydrogen bonds only with thymine and guanine would form three hydrogen bonds only with cyt ...
Section F
... Base excision repair (BER) • Base excision repair (BER): the lesion is removed by a specific DNA glycosylase, which cleave the N-glycosylic bond between the altered base and the sugar. This results AP site, then it is cleaved and expanded to a gap by ...
... Base excision repair (BER) • Base excision repair (BER): the lesion is removed by a specific DNA glycosylase, which cleave the N-glycosylic bond between the altered base and the sugar. This results AP site, then it is cleaved and expanded to a gap by ...
CHAPTER 10 TEST REVIEW - Hudson City School District
... #3 Name ways RNA is different from DNA: • 1. RNA = Ribose sugar instead of Deoxyribose in DNA • 2. DNA A-T C-G RNA A-U C-G • 3. RNA= Single stranded instead of Double in DNA • 4. RNA can go in and out of nucleus, DNA must stay in nucleus • 5. DNA can repair itself, RNA cannot ...
... #3 Name ways RNA is different from DNA: • 1. RNA = Ribose sugar instead of Deoxyribose in DNA • 2. DNA A-T C-G RNA A-U C-G • 3. RNA= Single stranded instead of Double in DNA • 4. RNA can go in and out of nucleus, DNA must stay in nucleus • 5. DNA can repair itself, RNA cannot ...
Ch. 9: Presentation Slides
... polymerase, reverse transcriptase, which uses a singlestranded RNA molecule as a template and synthesize a complementary DNA (cDNA). Like other DNA polymerases, reverse transcriptase requires a primer • The stretch of A nucleotides found at the 3' end of eukaryotic mRNA serves as a priming site. The ...
... polymerase, reverse transcriptase, which uses a singlestranded RNA molecule as a template and synthesize a complementary DNA (cDNA). Like other DNA polymerases, reverse transcriptase requires a primer • The stretch of A nucleotides found at the 3' end of eukaryotic mRNA serves as a priming site. The ...
CHAPTER 6
... Figure 28.6 The semidiscontinuous model for DNA replication. Newly synthesized DNA is shown as red. Because DNA polymerases only polymerize nucleotides 5 3, both strands must be synthesized in the 5 3 direction. Thus, the copy of the parental 3 5 strand is synthesized continuously; this ...
... Figure 28.6 The semidiscontinuous model for DNA replication. Newly synthesized DNA is shown as red. Because DNA polymerases only polymerize nucleotides 5 3, both strands must be synthesized in the 5 3 direction. Thus, the copy of the parental 3 5 strand is synthesized continuously; this ...
Molecular Genetics Test
... (3.) single nucleotide, only (4.) small polysaccharide (5.) male gamete 68. Structure B represents (1.) a ribosome (2.) recombinant DNA (3.) transfer RNA (4.) a female gamete 69. The technique illustrated in the diagram is known as (1.) cloning (2.) protein synthesis (3.) genetic engineering (4.) in ...
... (3.) single nucleotide, only (4.) small polysaccharide (5.) male gamete 68. Structure B represents (1.) a ribosome (2.) recombinant DNA (3.) transfer RNA (4.) a female gamete 69. The technique illustrated in the diagram is known as (1.) cloning (2.) protein synthesis (3.) genetic engineering (4.) in ...
TEXT Components of DNA To understand the structure of DNA, it is
... presence of proteins such as histones and non-histones. Discovery of DNA structure: The deoxyribonucleotides and their ability to form polynucleotide were discovered by Levene in 1931. But Levene postulated that the four deoxyribonucleotides occurred in a regularly repeated tetra nucleotide sequence ...
... presence of proteins such as histones and non-histones. Discovery of DNA structure: The deoxyribonucleotides and their ability to form polynucleotide were discovered by Levene in 1931. But Levene postulated that the four deoxyribonucleotides occurred in a regularly repeated tetra nucleotide sequence ...
Lectre 10
... Identify the roles of a clone and a vector in making recombined DNA. Define restriction enzymes, and outline how they are used to make recombinant DNA. Outline the steps in PCR and provide an example of its use. Describe how a gene library is made Differentiate cDNA from synthetic DNA. List the prop ...
... Identify the roles of a clone and a vector in making recombined DNA. Define restriction enzymes, and outline how they are used to make recombinant DNA. Outline the steps in PCR and provide an example of its use. Describe how a gene library is made Differentiate cDNA from synthetic DNA. List the prop ...
CLARK LAP Wednesday March 26 2014 STRAWBERRY DNA
... through the cheesecloth and into the tall glass until there is very little liquid left in the funnel (only wet pulp remains). How does the filtered strawberry liquid look? • Pour the filtered strawberry liquid from the tall glass into the small glass jar so that the jar is one quarter full. • Measur ...
... through the cheesecloth and into the tall glass until there is very little liquid left in the funnel (only wet pulp remains). How does the filtered strawberry liquid look? • Pour the filtered strawberry liquid from the tall glass into the small glass jar so that the jar is one quarter full. • Measur ...
I. Introduction: Definitions and mutation rates
... biological molecules; thus they are referred to as ionizing radiation. This term also includes corpuscular radiation--streams of atomic and subatomic particles emitted by radioactive elements: these are of two types, alpha- and beta-particles [alpha are helium nuclei, 2 protons and 2 neutrons; beta ...
... biological molecules; thus they are referred to as ionizing radiation. This term also includes corpuscular radiation--streams of atomic and subatomic particles emitted by radioactive elements: these are of two types, alpha- and beta-particles [alpha are helium nuclei, 2 protons and 2 neutrons; beta ...
Section 13-2
... sheet of paper, construct a table that has the following three heads: the name of each of the two dog breeds, and “Cross-Breed.” 2. The rows of the table should be labeled with characteristics found in both breeds of dogs. Examples might include size, color, type of coat, intelligence, aggression, a ...
... sheet of paper, construct a table that has the following three heads: the name of each of the two dog breeds, and “Cross-Breed.” 2. The rows of the table should be labeled with characteristics found in both breeds of dogs. Examples might include size, color, type of coat, intelligence, aggression, a ...
Chapter 14 Transposons, Plasmids, and Bacteriophage
... – not by transposase but by resolvase (product of tapR gene) – Recombination step (resolution) occurs at IRS sites in paired copies in cointegrate called res ...
... – not by transposase but by resolvase (product of tapR gene) – Recombination step (resolution) occurs at IRS sites in paired copies in cointegrate called res ...
Quizzes
... If two lambda left or two lambda right arms ligate to each other during preparation of a genomic library, the product will not be packaged into phage. Explain. This question was not graded. In the case of two lambda rights, the product would definitely be too short for packaging. The same may also b ...
... If two lambda left or two lambda right arms ligate to each other during preparation of a genomic library, the product will not be packaged into phage. Explain. This question was not graded. In the case of two lambda rights, the product would definitely be too short for packaging. The same may also b ...
in DNA? - Rufus King Biology
... Your cells replicate their DNA before they divide to make new cells. They do this… For routine replacement of cells (such as skin cells, blood cells, stomach cells, etc) When you grow or gain weight When you are injured and need to replace dead cells ...
... Your cells replicate their DNA before they divide to make new cells. They do this… For routine replacement of cells (such as skin cells, blood cells, stomach cells, etc) When you grow or gain weight When you are injured and need to replace dead cells ...
CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA
... disrupting lipid membranes with detergents à destroying proteins with phenol or proteases à degrading RNAs with RNase à leaving DNA at the end ...
... disrupting lipid membranes with detergents à destroying proteins with phenol or proteases à degrading RNAs with RNase à leaving DNA at the end ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.