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Microbial Genetics
Microbial Genetics

... During replication, enzymes known as polymerases transport nucleotides from the cytoplasm that are complimentary to the template and fit them into place, resulting in two strands, one parental and one new one. The replication is said to be semiconservative because the parental strand is conserved ( ...
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... Genes in cells are made of DNA, which is a complex molecule. The structure of a DNA molecule contains the information that a cell needs to carry out all of its functions. In a way, DNA is like the cell’s encyclopedia. Suppose that you go to the library to do research for a science project. You find ...
GENE SEQUENCING - Amirkabir University of Technology
GENE SEQUENCING - Amirkabir University of Technology

... • After the bases are "read," computers are used to assemble the short sequences (in blocks of about 500 bases each, called the read length) into long continuous stretches that are analyzed for errors, gene-coding regions, and other characteristics. • Finished sequence is submitted to major public s ...
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Protein Synthesis - science4warriors

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DNA Nucleotides Nitrogenous Base Complimentary Base Pairs

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... Alleles at the Molecular Level Each form of a gene is an allele. The standard (wild type) and altered (mutant) forms of the gene associated with hemoglobin and sickle cell anemia provide an example. The DNA sequences of both alleles of the “hemoglobin gene” are 99.9% identical – a single nucleotide ...
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... 19. Where does Translation occur? 20. Where in the cell does transcription occur? 21. Where in the cell does translation occur? 22. When does translation begin? 23. What brings amino acids to the ribosome? 24. How many different amino acids can a tRNA carry? 25. What are the three unpaired bases at ...
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... or 800µl of plasma, as well as on genomic DNA from an unaffected and a positive control. On an unaffected DNA sample, restriction digest of the PCR product with BsrG1 will not cut the DNA, giving rise to a single 132bp fragment, whereas if the mutation is present a BsrG1 restriction site is created, ...
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...  The enzyme RNA polymerase unzips DNA  mRNA nucleotides bond with the template strand of DNA until it reaches a stop codon  mRNA leaves the nucleus and travels to the ribosome ...
DNA - Mr. Johnston's Biology Site
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...  The enzyme RNA polymerase unzips DNA  mRNA nucleotides bond with the template strand of DNA until it reaches a stop codon  mRNA leaves the nucleus and travels to the ribosome ...
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... the width of a human hair, but if you unwound the chromosomes, the DNA would be six feet long. All living things contain DNA recipes and use them to make proteins. This amazing commonality across all forms of life has made possible many practical uses of our DNA knowledge, some of which have been wi ...
Chapter 12 Exam Review
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... 1. Briefly explain how the following scientists contributed to the current understanding of DNA as the genetic material: Griffith, Avery, Chargaff, Franklin, Watson and Crick 2. Explain the process of DNA replication. When and why does it occur? What are the steps and the enzymes necessary to comple ...
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... strain could change the inherited characteristics of another strain. He called the process transformation because one type of bacteria (a harmless form) had been changed permanently into another (a disease-carrying form). Because the ability to cause disease was inherited by the offspring of the tra ...
restriction enzymes restriction enzymes
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DNA

... What is the structure of a gene? • Genes once thought to be made of protein • Chromosomes contain both DNA & protein (histones) • Cell structures are built of protein, cell function controlled by enzymes (protein) • Nucleus once thought to contain a “master molecule” for each of cell’s protein. ...
T4 DNA Polymerase
T4 DNA Polymerase

... Extensive labeling is achieved by the replacement reaction, in which the 3´-exonuclease activity of the enzyme first digests dsDNA to produce molecules with recessed 3´-termini (7). On subsequent addition of labeled dNTPs, the polymerase activity of T4 DNA polymerase then extends the 3´-ends along t ...
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DNA polymerase



The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.
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