GENETIC TECHNOLOGY
... To eliminate recircularized vectors from further examination, lacZ gene part of vector Insertion of chromosomal DNA disrupts lacZ gene lacZ codes for b-galactosidase which cleaves colorless X-Gal into a blue dye Recircularized plasmids will form blue colonies Recombinant vectors will form white colo ...
... To eliminate recircularized vectors from further examination, lacZ gene part of vector Insertion of chromosomal DNA disrupts lacZ gene lacZ codes for b-galactosidase which cleaves colorless X-Gal into a blue dye Recircularized plasmids will form blue colonies Recombinant vectors will form white colo ...
Promoters - Pennsylvania State University
... • Use site-directed mutations (deletions and point mutations) in the DNA sequence to test promoter activity. • Ligate the mutated DNA fragments to the coding region of a reporter gene. – Any gene: assay for stable RNA whose 5’ end is at the start site for transcription. – beta-galactosidase: measure ...
... • Use site-directed mutations (deletions and point mutations) in the DNA sequence to test promoter activity. • Ligate the mutated DNA fragments to the coding region of a reporter gene. – Any gene: assay for stable RNA whose 5’ end is at the start site for transcription. – beta-galactosidase: measure ...
lecture_11(LP)
... -The textbooks that are on reserve in the undergraduate library cover these techniques very well: -Chapter 20 (specifically, pages 715-735) in Griffiths, et al. -Chapter 9 (specifically, pages 301-335) in Hartwell, et al. -Chapter 19 in Klug, et al. -I have provided links on the course website that ...
... -The textbooks that are on reserve in the undergraduate library cover these techniques very well: -Chapter 20 (specifically, pages 715-735) in Griffiths, et al. -Chapter 9 (specifically, pages 301-335) in Hartwell, et al. -Chapter 19 in Klug, et al. -I have provided links on the course website that ...
BIOL 105 S 2013 Practice Quiz Supp DNA
... Messenger RNA carries genetic information from the chromosomes to the ______. A) ribosomes B) endoplasmic reticulum C) nucleolus D) plasmids Answer A A ________ codes for a specific sequence of amino acids in a protein. A) gene B) nucleotide C) polyribosome D) nucleic acid Answer A The study of the ...
... Messenger RNA carries genetic information from the chromosomes to the ______. A) ribosomes B) endoplasmic reticulum C) nucleolus D) plasmids Answer A A ________ codes for a specific sequence of amino acids in a protein. A) gene B) nucleotide C) polyribosome D) nucleic acid Answer A The study of the ...
Exam3-1406_Spring'06.doc
... 25) The sequence of nitrogencontaining bases on one strand of DNA most directly determines the sequence of A) fatty acids in a fat molecule. B) amino acids in a protein molecule. C) sugars in a polysaccharide molecule. D) All of the above choices are correct. E) bases in a protein molecule. 26) Both ...
... 25) The sequence of nitrogencontaining bases on one strand of DNA most directly determines the sequence of A) fatty acids in a fat molecule. B) amino acids in a protein molecule. C) sugars in a polysaccharide molecule. D) All of the above choices are correct. E) bases in a protein molecule. 26) Both ...
MICR 130 Chapter 8
... nucleotide sequence of a gene is converted into the amino acid sequence of a protein Linear sequence of nucleotides, bases provides the information for making proteins Much of anabolism is making proteins from DNA When a product is made, the gene is expressed ...
... nucleotide sequence of a gene is converted into the amino acid sequence of a protein Linear sequence of nucleotides, bases provides the information for making proteins Much of anabolism is making proteins from DNA When a product is made, the gene is expressed ...
Document
... adenine, thymine, cytosine, guanine • Double helix associated with proteins • "Backbone" is deoxyribose-phosphate • Strands held together by hydrogen bonds between base pairs (A-T and C-G) • Strands are antiparallel ...
... adenine, thymine, cytosine, guanine • Double helix associated with proteins • "Backbone" is deoxyribose-phosphate • Strands held together by hydrogen bonds between base pairs (A-T and C-G) • Strands are antiparallel ...
Document
... In 1950, after analyzing the data, Erwin Chargaff reported that even though the DNA composition varied from one species to another he suggested that there was a pairing of complementary nucleotide bases (A to T and G to C) in the DNA molecule. ...
... In 1950, after analyzing the data, Erwin Chargaff reported that even though the DNA composition varied from one species to another he suggested that there was a pairing of complementary nucleotide bases (A to T and G to C) in the DNA molecule. ...
No Slide Title - Fort Bend ISD
... Sickle-cell anemia is a disorder resulting from a mutation that leads to the production of an abnormal protein. Which component of the DNA molecule provides instructions for the production of the protein? a. The phosphate groups b. The sugar molecules c. The sequence of nitrogen bases d. The bonds ...
... Sickle-cell anemia is a disorder resulting from a mutation that leads to the production of an abnormal protein. Which component of the DNA molecule provides instructions for the production of the protein? a. The phosphate groups b. The sugar molecules c. The sequence of nitrogen bases d. The bonds ...
Mitochondrial Deoxyribonucleic Acids in a Range of
... inhibits any DNase activity and also greatly increases the yield of DNA, particularly from older leaf tissue. The salt concentration was increased to 0.5 M, and an equal volume of chloroform3-methylbutanol-1 (24: 1, v/v) was added. The mixture was shaken vigorously for 10 min and centrifuged at 2,50 ...
... inhibits any DNase activity and also greatly increases the yield of DNA, particularly from older leaf tissue. The salt concentration was increased to 0.5 M, and an equal volume of chloroform3-methylbutanol-1 (24: 1, v/v) was added. The mixture was shaken vigorously for 10 min and centrifuged at 2,50 ...
Reproduction DNA
... Just 46 strands of DNA? DNA is the _________________________________________________________________________ Your body makes about 35,000 different proteins Why do we only have 46 strands of DNA and not 35,000? One chromosome or one strand of DNA has the instructions to make hundreds to thous ...
... Just 46 strands of DNA? DNA is the _________________________________________________________________________ Your body makes about 35,000 different proteins Why do we only have 46 strands of DNA and not 35,000? One chromosome or one strand of DNA has the instructions to make hundreds to thous ...
Plasmid Isolation Using Alkaline Lysis
... The plasmid "miniprep " method is useful for preparing partially purified plasmid DNA in small quantities from a number of transformants. It relies on an alkaline SDS lysis to free the plasmid DNA from the cell, leaving behind the E. coli chromosomal DNA with cell wall debris. The protocol described ...
... The plasmid "miniprep " method is useful for preparing partially purified plasmid DNA in small quantities from a number of transformants. It relies on an alkaline SDS lysis to free the plasmid DNA from the cell, leaving behind the E. coli chromosomal DNA with cell wall debris. The protocol described ...
DNA Fingerprinting powerpoint
... What is the primary difference between coding DNA and non-coding DNA? A. Coding DNA is made of all four possible DNA nucleotides; non-coding DNA contains only three of the possible four nucleotides. B. Coding DNA is less susceptible to mutation than is non-coding DNA. C. Non-coding DNA does not cont ...
... What is the primary difference between coding DNA and non-coding DNA? A. Coding DNA is made of all four possible DNA nucleotides; non-coding DNA contains only three of the possible four nucleotides. B. Coding DNA is less susceptible to mutation than is non-coding DNA. C. Non-coding DNA does not cont ...
Chapter Outline with All Images
... many forms of cancer. These mice were designed for studying cancer development and the design of new anticancer drugs. ...
... many forms of cancer. These mice were designed for studying cancer development and the design of new anticancer drugs. ...
Genetics Option - Worked Examples
... Positive identification is less certain, even if there is a perfect match between two DNA samples. At best, this can provide an extremely high probability that the samples came from the same source. The reason for this is that a number of people may show the same patterns on a DNA test. The probabi ...
... Positive identification is less certain, even if there is a perfect match between two DNA samples. At best, this can provide an extremely high probability that the samples came from the same source. The reason for this is that a number of people may show the same patterns on a DNA test. The probabi ...
eprint_12_13279_954
... These are not essential for the survival of the bacterium but they confer certain extra advantages to the cell. Number and size: A bacterium can have no plasmids at all or have many plasmids (20-30) or multiple copies of a plasmid. Usually they are closed circular molecules; however they occur as li ...
... These are not essential for the survival of the bacterium but they confer certain extra advantages to the cell. Number and size: A bacterium can have no plasmids at all or have many plasmids (20-30) or multiple copies of a plasmid. Usually they are closed circular molecules; however they occur as li ...
Lecture 6 - EukDNAexpression2007 - Cal State LA
... Gene activator activity for genes in the host cell that are involved in controlling the host cell cycle. Cellular DNA synthesis is induced so the the enzymes that the virus requires to replicate its genome are available. Large T Ags presence in tissue culture cells can result in immortalizatio ...
... Gene activator activity for genes in the host cell that are involved in controlling the host cell cycle. Cellular DNA synthesis is induced so the the enzymes that the virus requires to replicate its genome are available. Large T Ags presence in tissue culture cells can result in immortalizatio ...
Plasmid Isolation
... • Each restriction enzyme cuts at different sites • The cut can leave either blunt or sticky ends • If the plasmid and gene to be inserted into the plasmid are cut with the same restriction enzyme, the sticky ends have overlapping base pairs and can anneal with DNA ligase • But, the cut ends can ann ...
... • Each restriction enzyme cuts at different sites • The cut can leave either blunt or sticky ends • If the plasmid and gene to be inserted into the plasmid are cut with the same restriction enzyme, the sticky ends have overlapping base pairs and can anneal with DNA ligase • But, the cut ends can ann ...
Transcription and Translation Candy
... Make a model demonstrating the process of translation. Your model should include where in the cell translation takes place and the final product of translation. Your model should include the following: ribosomes, codons, tRNA with anti-codons and appropriate amino acid, forming polypeptide and compl ...
... Make a model demonstrating the process of translation. Your model should include where in the cell translation takes place and the final product of translation. Your model should include the following: ribosomes, codons, tRNA with anti-codons and appropriate amino acid, forming polypeptide and compl ...
Exploring DNA Structures
... Background Information: DNA is the basic material that contains the information that is responsible for the way all living organisms physically look and instruction on how to carry out the activities of the cell. We are going to explore the different parts of DNA. READ THIS BEFORE MOVING ON: Before ...
... Background Information: DNA is the basic material that contains the information that is responsible for the way all living organisms physically look and instruction on how to carry out the activities of the cell. We are going to explore the different parts of DNA. READ THIS BEFORE MOVING ON: Before ...
Replication - UniMAP Portal
... Next, an enzyme called DNA helicase locally "unzips" the DNA molecule by breaking the hydrogen bonds between complementary nucleotide bases, which exposes the bases in a replication fork. Other protein molecules stabilize the single strands so that they do not rejoin while replication proceeds After ...
... Next, an enzyme called DNA helicase locally "unzips" the DNA molecule by breaking the hydrogen bonds between complementary nucleotide bases, which exposes the bases in a replication fork. Other protein molecules stabilize the single strands so that they do not rejoin while replication proceeds After ...
DNA Replication - Crestwood Local Schools
... • Enzymes proofread and correct these mistakes • The new error rate for DNA that has been proofread is 1 in 1 billion base pairing errors copyright cmassengale ...
... • Enzymes proofread and correct these mistakes • The new error rate for DNA that has been proofread is 1 in 1 billion base pairing errors copyright cmassengale ...
Transcription, translation and mutations Lab 7
... There are notable differences between DNA and RNA molecules. DNA molecules are double stranded, whereas RNA is typically single stranded. DNA nucleotides contain a deoxyribose sugar; RNA nucleotides contain a ribose sugar. The nitrogenous bases of DNA nucleotides are adenine (A), thymine (T), cytosi ...
... There are notable differences between DNA and RNA molecules. DNA molecules are double stranded, whereas RNA is typically single stranded. DNA nucleotides contain a deoxyribose sugar; RNA nucleotides contain a ribose sugar. The nitrogenous bases of DNA nucleotides are adenine (A), thymine (T), cytosi ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.