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Biochemistry - Problem Drill 22: DNA Question No. 1 of 10
Biochemistry - Problem Drill 22: DNA Question No. 1 of 10

... (C) The diameter of the helix is 20 Å. Adjacent bases are separated by 3.4 Å along the helix axis and related by a rotation of 36 degrees. Hence, the helical structure repeats after 10 residues on each chain, that is, at interval of 34 Å. (D) The two chains are held together by phosphate bonds betwe ...
Transcription and translation ppt
Transcription and translation ppt

... depends on complementary base pairing. Helicase unwinds the double helix and separates the two strands by breaking hydrogen bonds. DNA polymerase links nucleotides together to form The different types of DNA polymerase do not a new strand, using the pre-existing strand as a need to be distinguished. ...
EOC Review 2011 #3
EOC Review 2011 #3

... j. What factors could speed up (or slow down) process A? AVAILABLE LIGHT, CO2, TEMP, PH k. What factors could speed up (or slow down) process B? AMT OF AVAILABLE GLUCOSE/O2, TEMP, PH ...
Isolation and characterization of a functional promoter from
Isolation and characterization of a functional promoter from

... L and RI and primers L and R2 amplified only the expected DNA fragments, 765 and 470 bp respectively. from 1.0 ng of total genomic DNA of N. europaeu (Fig. 2). Based on DNA sequences of pKA16, the 470 bp fragment does not contain a restriction site for PstI while the 765 bp fragment contains two res ...
Extracting DNA from Your Cells
Extracting DNA from Your Cells

... Draw a rectangle around a single nucleotide in the double helix. 2. The complete name for DNA is deoxyribonucleic acid. Which component of each nucleotide accounts for the "deoxyribo” part of this name? ...
DNA
DNA

... Both parental strands serve as templates ...
Viral capsid proteins bind to receptors on host cell membrane (outer
Viral capsid proteins bind to receptors on host cell membrane (outer

... 1. Print and laminate the first page in one color. Print this second page in a different color. To make a full set, cut all from page 1 and 1 of each from page 2. Have groups of 2 sequence page one only, the lytic cycle. Have them put the different colored cards aside. 2. Once each group has complet ...
Transcription of DNA into RNA
Transcription of DNA into RNA

... DNA is transcribed by an enzyme called RNA  polymerase.  The enzyme pries apart the two DNA  strands and starts transcribing the template strand  (diagram 1).  Specific nucleotide sequences tell RNA  polymerase where to begin and where to end on the  DNA.  These sequences are not included in this  m ...
Quiet debut for the double helix
Quiet debut for the double helix

... of enzymes to both synthesize and break down their substrates, with a high degree of specificity attributed to both actions. Synthesis was proposed to involve the formation of a succession of peptides, ultimately yielding the protein molecule, and enzymes synthesize only those peptide bonds that the ...
Biology DNA: The Genetic Material
Biology DNA: The Genetic Material

... The process of making a copy of DNA is called DNA replication. It occurs during the synthesis (S) phase of the cell cycle, before a cell divides. The process can be broken down into three steps. Step 1: Before replication can begin, the double helix must unwind. This is accomplished by enzymes calle ...
18. Introduction to Metagenomes
18. Introduction to Metagenomes

... • Sequence coverage can be computed by the assembler based on alignments it generates (preferable) or can be added later by aligning reads to contigs – the latter can be provided in IMG/M • Bins are generated by binning software – not provided in IMG/M • Scaffolds, contigs and unassembled reads are ...
Chapter 15 DNA: The Indispensable Forensic Science Tool
Chapter 15 DNA: The Indispensable Forensic Science Tool

... STRs are locations on the chromosome that contain short sequences that repeat themselves within the DNA molecule. They serve as useful markers for identification because they are found in great abundance throughout the human genome. ...
Plasmid w/ kanamycin resistance (pKAN)
Plasmid w/ kanamycin resistance (pKAN)

... 2008 Data/Results ...
Unit 5, pt 1: Chapter Objectives: from C Massengale – Biology
Unit 5, pt 1: Chapter Objectives: from C Massengale – Biology

... 6. Describe the process of DNA replication, including the role of the origins of replication and replication forks. 7. Explain the role of DNA polymerases in replication. 8. Explain what energy source drives the polymerization of DNA. 9. Define antiparallel and explain why continuous synthesis of bo ...
No Slide Title
No Slide Title

... nearly every round of DNA replication that interrupt the replication fork and require general recombination mechanisms to repair. • Cross-over during meiosis also use the same mechanism of general recombination. ...
When replication travels on damaged templates: bumps and blocks
When replication travels on damaged templates: bumps and blocks

... Following a single priming event, the leading-strand template can be synthesized in a continuous, processive 5 –3 manner. However, the lagging strand template is synthesized in a direction opposite to the progress of the ongoing fork, and requires a primase activity that must constantly reprime th ...
DNA is
DNA is

... If the diameter of the DNA (2 nanometers) was as wide as a fishing line (0.5 millimeters) it might stretch as far as 21.2 km (or 13.6 miles) in length which would all have to be packed into a nucleus, the equivalent size of 25 cm in diameter. ...
Molecular Biology I
Molecular Biology I

... 4) Injected mice with a mixture of live R strain and heat killed S strain  mice died These results from experiment 4 were surprising because neither the R strain nor the heat killed S strain was virulent when give independently. Girffith isolated the blood from the dead mouse and cultured it. He fo ...
Chapter 10
Chapter 10

... An enzyme separates a region of DNA and then copies it billions of times Samples are heated and cooled in a three-step cycle • Denaturing—(heating) separating the two DNA strands • Primer annealing—(cooling) adding primers (markers) to the beginning and ends of targeted sequences • Polymerization—(h ...
Section 1: The Structure of DNA
Section 1: The Structure of DNA

... often 10 times the size of a prokaryotic chromosome. Eukaryotic chromosomes are so long that it would take 33 days to replicate a typical human chromosome if there were only one origin of replication. ...
DNA - UCSF Tetrad Program
DNA - UCSF Tetrad Program

... repair. Fewer molecules are needed for the 2 replication forks, so the residual activity in a polA1 mutant may be sufficient. Note, although polA1 has an early nonsense mutation, read-through of the nonsense codon is suspected of generating the residual Pol I activity ...
Test File
Test File

... direction of replication. c. The Okazaki fragments are joined by the action of DNA ligase. d. Both strands are synthesized continuously at the replication fork. 14. DNA polymerase requires a primer and cannot initiate synthesis de novo. What serves as a primer for DNA replication? a. Short fragments ...
Water - The Burge
Water - The Burge

... III. Water has Unique Characteristics • It is abundant through the biosphere • Hydrogen bonding makes it have a low freezing point and a high boiling point, so that it is liquid at body temperature • Water absorbs much heat before it warms up or boils, and gives off much heat before it freezes beca ...
Chapter 12 : DNA Summary
Chapter 12 : DNA Summary

... Avery and the other scientists repeated the experiment, except this using enzymes that would break down DNA.  When they destroyed the nucleic acid DNA in the extract, transformation did not occur. ...
DNA-1 - Ryler Enterprises, Inc
DNA-1 - Ryler Enterprises, Inc

... Three features of DNA can be seen after making the model. First, there are two kinds of bonds. The clear, thicker tubes represent strong-chemical bonds that can occur between almost any two types of atoms. The longer, white tubes are for hydrogen bonds that are weaker and involve the sharing of hydr ...
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DNA replication



DNA replication is the process of producing two identical replicas from one original DNA molecule. This biological process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of two strands and each strand of the original DNA molecule serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication.In a cell, DNA replication begins at specific locations, or origins of replication, in the genome. Unwinding of DNA at the origin and synthesis of new strands results in replication forks growing bidirectional from the origin. A number of proteins are associated with the replication fork which helps in terms of the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new DNA by adding complementary nucleotides to the template strand.DNA replication can also be performed in vitro (artificially, outside a cell). DNA polymerases isolated from cells and artificial DNA primers can be used to initiate DNA synthesis at known sequences in a template DNA molecule. The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA.
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