VGEC: Student Handout Wear a Chimp on Your Wrist 1
... The gene sequence that was used for all the different organisms is the first 21 bases of the cytochrome b gene. The gene is involved in helping to release energy from food. Almost all life on earth gets energy from food in the same way, which is why this gene is found in everything from plants to hu ...
... The gene sequence that was used for all the different organisms is the first 21 bases of the cytochrome b gene. The gene is involved in helping to release energy from food. Almost all life on earth gets energy from food in the same way, which is why this gene is found in everything from plants to hu ...
Plasmids and DNA Digestion
... Vector: DNA (or RNA) used to artificially carry foreign material into another cell. Plasmid: Circular piece of double stranded DNA used as a vector for bacterial cells. A plasmid is a vector but not all vectors are plasmids. Multiple Cloning Site (MCS): A region of the plasmid containing many restri ...
... Vector: DNA (or RNA) used to artificially carry foreign material into another cell. Plasmid: Circular piece of double stranded DNA used as a vector for bacterial cells. A plasmid is a vector but not all vectors are plasmids. Multiple Cloning Site (MCS): A region of the plasmid containing many restri ...
Notes Packet - Ms. Ottolini`s Biology Wiki!
... multiple journals) for review. A process called peer review is used, in which other scientists evaluate the methods used in the research and question the legitimacy of the results and conclusions. If the article is deemed legitimate, it may be accepted by the journal and published. ...
... multiple journals) for review. A process called peer review is used, in which other scientists evaluate the methods used in the research and question the legitimacy of the results and conclusions. If the article is deemed legitimate, it may be accepted by the journal and published. ...
DNA, The Genetic Material
... genetic material to generate an individual. The term given for this is TOTIPOTENT. The Griffith Experiment – Which of the two, proteins or DNA, held the genes for heredity? British biologist started work in 1928 experimenting with a pathogenic bacteria, Streptococcus pneumoniae, and continued for 30 ...
... genetic material to generate an individual. The term given for this is TOTIPOTENT. The Griffith Experiment – Which of the two, proteins or DNA, held the genes for heredity? British biologist started work in 1928 experimenting with a pathogenic bacteria, Streptococcus pneumoniae, and continued for 30 ...
Activity 4.5: Forensic DNA Fingerprinting
... When setting up restriction digests use fresh tips each time to prevent contamination Tubes can be incubated in a water bath, dry bath, or at room temperature overnight – If incubating overnight, it is helpful to incubate for a short while at 37ºC first, then let come to room temperature overnig ...
... When setting up restriction digests use fresh tips each time to prevent contamination Tubes can be incubated in a water bath, dry bath, or at room temperature overnight – If incubating overnight, it is helpful to incubate for a short while at 37ºC first, then let come to room temperature overnig ...
Nylon/DNA: Single-Stranded DNA with a Covalently Stitched Nylon
... assemble organic materials with unique structures. Our approach also entails regiospecific chemistry between non-DNA entities. Here, we report the first nucleic acid-based structure in which a DNA backbone has been covalently linked to an organic polymer, nylon. The synthesis was accomplished in thr ...
... assemble organic materials with unique structures. Our approach also entails regiospecific chemistry between non-DNA entities. Here, we report the first nucleic acid-based structure in which a DNA backbone has been covalently linked to an organic polymer, nylon. The synthesis was accomplished in thr ...
... Traditionally, Southern blots have been used to determine gene copy number (Southern, 1975). This typically involves extracting a significant quantity of genomic DNA, undergoing restriction digestions prior to blotting and probing. This is time consuming and often involves the use of 32P. Since its ...
Gene Cloning
... This is done by using an oligo dT column or oligo dT magnetic beads to isolated mRNA which is polyadenylated. cDNA synthesis then relies upon the enzyme Reverse transcriptase and a primer, usually an oligo dT primer for first strand synthesis and then a self-priming or specific primer plus a DNA pol ...
... This is done by using an oligo dT column or oligo dT magnetic beads to isolated mRNA which is polyadenylated. cDNA synthesis then relies upon the enzyme Reverse transcriptase and a primer, usually an oligo dT primer for first strand synthesis and then a self-priming or specific primer plus a DNA pol ...
DNA Technology ppt chapter 13 Honors Txtbk
... Distinctive pattern of STR numbers and lengths are fairly unique to a specific individual (forming a DNA fingerprint) DNA fingerprint from crime scene can be matched with DNA fingerprint of suspect ...
... Distinctive pattern of STR numbers and lengths are fairly unique to a specific individual (forming a DNA fingerprint) DNA fingerprint from crime scene can be matched with DNA fingerprint of suspect ...
Overview of recombinant technology
... UvrAB proteins identify the bulky dimer lesion, UvrA protein then leaves, and UvrC protein then binds to UvrB protein and introduces the nicks on either side of the dimer. In man there is a similar process carried out by 2 related enzyme complexes: global excision repair and transcription coupled re ...
... UvrAB proteins identify the bulky dimer lesion, UvrA protein then leaves, and UvrC protein then binds to UvrB protein and introduces the nicks on either side of the dimer. In man there is a similar process carried out by 2 related enzyme complexes: global excision repair and transcription coupled re ...
CHEM642-14 Powerpoint
... marker such as a fluorescent dye. Other chemical labels such as biotin can be attached to nucleotides and used in essentially the same way. ...
... marker such as a fluorescent dye. Other chemical labels such as biotin can be attached to nucleotides and used in essentially the same way. ...
Test 2
... DNA binding protein comes in to prevent the unwound DNA from winding back up The DnaB then serves as the start of the DNA polymerase complex that will include DNA gyrase and primase a well as DNA polymerase, but that is considered part of the elongation step This process only occurs once in the cell ...
... DNA binding protein comes in to prevent the unwound DNA from winding back up The DnaB then serves as the start of the DNA polymerase complex that will include DNA gyrase and primase a well as DNA polymerase, but that is considered part of the elongation step This process only occurs once in the cell ...
Examination Issues when Claiming Microarrays
... • Do examination issues for microarrays differ from oligonucleotides per se? • Conventional DNA microarrays are designed using oligonucleotide probes or complementary DNA (cDNA). • Oligonucleotide can be synthesized in situ or via conventional DNA synthesis and later attached to the chip surface. ...
... • Do examination issues for microarrays differ from oligonucleotides per se? • Conventional DNA microarrays are designed using oligonucleotide probes or complementary DNA (cDNA). • Oligonucleotide can be synthesized in situ or via conventional DNA synthesis and later attached to the chip surface. ...
Chapter-12 PTT
... Short Tandem Repeat (STR) Analysis- used to compare genomes in two samples to prove they came from the same person. This is used by a series of short sequences (Repetitive DNA) that is repeated many times one after another. ...
... Short Tandem Repeat (STR) Analysis- used to compare genomes in two samples to prove they came from the same person. This is used by a series of short sequences (Repetitive DNA) that is repeated many times one after another. ...
Lectre 10
... Identify the roles of a clone and a vector in making recombined DNA. Define restriction enzymes, and outline how they are used to make recombinant DNA. Outline the steps in PCR and provide an example of its use. Describe how a gene library is made Differentiate cDNA from synthetic DNA. List the prop ...
... Identify the roles of a clone and a vector in making recombined DNA. Define restriction enzymes, and outline how they are used to make recombinant DNA. Outline the steps in PCR and provide an example of its use. Describe how a gene library is made Differentiate cDNA from synthetic DNA. List the prop ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.