analysis
... a) These lack a 3' hydroxyl group, so when incorporated polymerization stops b) Each of the four reaction mixtures will have a different dideoxynucleotide (ddGTP, ddATP, ddCTP, or ddTTP) C. Electrophoresis 1. Denature the DNA before electrophoresis 2. Each reaction mixture will be electrophoresed in ...
... a) These lack a 3' hydroxyl group, so when incorporated polymerization stops b) Each of the four reaction mixtures will have a different dideoxynucleotide (ddGTP, ddATP, ddCTP, or ddTTP) C. Electrophoresis 1. Denature the DNA before electrophoresis 2. Each reaction mixture will be electrophoresed in ...
Mutations
... A mutation is a sudden change in the genetic material. Gene mutations occur randomly and can occur in any cell. Mutations can happen accidentally or can be caused by radiation or toxic chemicals. A mutation in a single body cell does not ...
... A mutation is a sudden change in the genetic material. Gene mutations occur randomly and can occur in any cell. Mutations can happen accidentally or can be caused by radiation or toxic chemicals. A mutation in a single body cell does not ...
Unit 5 Applied Genetics Notes
... • Some success in treating cystic fibrosis has been seen using gene therapy. A nasal spray that carries a normal cystic fibrosis gene can be sprayed in the nose and delivered to the lungs where it is absorbed by the nucleus of the cells. The treatment must be repeated periodically because the gene ...
... • Some success in treating cystic fibrosis has been seen using gene therapy. A nasal spray that carries a normal cystic fibrosis gene can be sprayed in the nose and delivered to the lungs where it is absorbed by the nucleus of the cells. The treatment must be repeated periodically because the gene ...
The Human Genome Analysis Variable Number Tandem Repeats
... cDNA from breast tumors breast cancer patients ...
... cDNA from breast tumors breast cancer patients ...
wave genetics verbatim
... The wave emitting genes of this “fine field” level are called the “super-gene-continuum”, but there is no sharp distinction between genes and super-genes. There is a fundamental similarity, or unity, between fractal (replicating itself on increasing or decreasing scales) sequence structures of DNA ...
... The wave emitting genes of this “fine field” level are called the “super-gene-continuum”, but there is no sharp distinction between genes and super-genes. There is a fundamental similarity, or unity, between fractal (replicating itself on increasing or decreasing scales) sequence structures of DNA ...
Semester Exam Review
... Know cell organelles and their functions Which organelles contain DNA Diffusion Phospholipids Lactic acid First and second law of Thermodynamics Relationship of monomers to polymers. Give examples Difference between RNA and DNA What is the difference between active and passive transport? Give exampl ...
... Know cell organelles and their functions Which organelles contain DNA Diffusion Phospholipids Lactic acid First and second law of Thermodynamics Relationship of monomers to polymers. Give examples Difference between RNA and DNA What is the difference between active and passive transport? Give exampl ...
File
... 3. What is a gene? ________________________________________________________. 4. Look at Figure 4.5 on page 126. On this diagram, DNA is shown as a twisted ladder. The sides of the ladder consist of long chains made up of “P”s and “S”s. Each “P” stands for a ________________ and each “S” stands for a ...
... 3. What is a gene? ________________________________________________________. 4. Look at Figure 4.5 on page 126. On this diagram, DNA is shown as a twisted ladder. The sides of the ladder consist of long chains made up of “P”s and “S”s. Each “P” stands for a ________________ and each “S” stands for a ...
DNA as Videotape: Introductory Fact Sheet
... splice it electronically into a copy of a second movie. We can make copies of the edited tapes. Tapes of two different movies are composed of the same tape, but the information recorded in them is different. Tapes can come in different formats: for example, VHS and Beta. ...
... splice it electronically into a copy of a second movie. We can make copies of the edited tapes. Tapes of two different movies are composed of the same tape, but the information recorded in them is different. Tapes can come in different formats: for example, VHS and Beta. ...
presentation source
... fragment length polymorphisms (RFLPs): point mutations specific to individuals causes different ‘banding’ in an electrophoresis gel • DNA can be sequenced using the Sanger method. Primers (deoxynucleotides and dioxynucleotides) are added to DNA fragments. Depending on nucleotide sequence, primers re ...
... fragment length polymorphisms (RFLPs): point mutations specific to individuals causes different ‘banding’ in an electrophoresis gel • DNA can be sequenced using the Sanger method. Primers (deoxynucleotides and dioxynucleotides) are added to DNA fragments. Depending on nucleotide sequence, primers re ...
LEQ: How do we splice new genes into DNA?
... paper). Apply radioactive probe designed to detect (bind to) harmful allele / gene of interest. Unattached probes are rinsed off. Photographic film used to form a image that compares individuals. In this picture I had the harmful allele. If any individual matches the banding pattern of I, then they ...
... paper). Apply radioactive probe designed to detect (bind to) harmful allele / gene of interest. Unattached probes are rinsed off. Photographic film used to form a image that compares individuals. In this picture I had the harmful allele. If any individual matches the banding pattern of I, then they ...
Electrochemical DNA Biosensors
... Watterson, J. H., Piunno, P. A. E., Wust, C. C., Krull, U. J. (2000) Langmuir 16, ...
... Watterson, J. H., Piunno, P. A. E., Wust, C. C., Krull, U. J. (2000) Langmuir 16, ...
Lecture 14
... i. One copy of DNA info for energy and copying again ii. Two copies of DNA division, result in two cells iii. Cycle repeats c. DNA info persistent, as individual cells die, information lives on in new cells III. How DNA info is arranged a. Genes: units making one protein b. Chromosome: units of ...
... i. One copy of DNA info for energy and copying again ii. Two copies of DNA division, result in two cells iii. Cycle repeats c. DNA info persistent, as individual cells die, information lives on in new cells III. How DNA info is arranged a. Genes: units making one protein b. Chromosome: units of ...
Genetic_Engineers_Mini
... Process by which multiple copies of a gene are produced in a bacterium or bacteriophage ...
... Process by which multiple copies of a gene are produced in a bacterium or bacteriophage ...
Genetics Syllabus
... Case Workbook in Human Genetics: Blue Diaper Syndrome p.21 Acrocephalosyndactyly p. 1 Available Equipment and Materials, Storage Location: Cultural materials are stored in 813 Need to purchase: seeds, soil, fly cultures ...
... Case Workbook in Human Genetics: Blue Diaper Syndrome p.21 Acrocephalosyndactyly p. 1 Available Equipment and Materials, Storage Location: Cultural materials are stored in 813 Need to purchase: seeds, soil, fly cultures ...
Guided Notes – Genetic Engineering
... Transgenic Organisms: organisms that contain some genes from other organisms o Transgenic bacteria now produce a host of important _____________________ useful for __________________ & _______________________. Human insulin, growth hormone, and clotting factor are now produced by transgenic bacter ...
... Transgenic Organisms: organisms that contain some genes from other organisms o Transgenic bacteria now produce a host of important _____________________ useful for __________________ & _______________________. Human insulin, growth hormone, and clotting factor are now produced by transgenic bacter ...
Why is DNA called the "blueprint of life"?
... Describe the three components of a nucleotide. Develop a model of the structure of a DNA molecule. Evaluate the contributions of Chargaff, Franklin, and Wilkins in helping Watson and Crick determine the double-helical structure of DNA. Relate the role of the base pairing rules to the structure of DN ...
... Describe the three components of a nucleotide. Develop a model of the structure of a DNA molecule. Evaluate the contributions of Chargaff, Franklin, and Wilkins in helping Watson and Crick determine the double-helical structure of DNA. Relate the role of the base pairing rules to the structure of DN ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.