Selective Breeding and Genetic Engineering
... according to size Recombinant DNA: Creating DNA molecules (plasmids) with portions from more than one organism Polymerase Chain Reaction (PCR): Creating multiple copies of a short segment of DNA in a test tube ...
... according to size Recombinant DNA: Creating DNA molecules (plasmids) with portions from more than one organism Polymerase Chain Reaction (PCR): Creating multiple copies of a short segment of DNA in a test tube ...
DNA Replication
... Name: _____________ Period: ___ Date:________ (3) As you know, DNA is found within the vacuole of the cell. In order for each cell to function properly, it must have the correct amount of DNA. So, before cells divide, the DNA must replicate. DNA replication is kind of tricky, though, because the squ ...
... Name: _____________ Period: ___ Date:________ (3) As you know, DNA is found within the vacuole of the cell. In order for each cell to function properly, it must have the correct amount of DNA. So, before cells divide, the DNA must replicate. DNA replication is kind of tricky, though, because the squ ...
DNA Notes
... • Was able to tell that DNA had an “X” like structure where the strands are twisted around each other • All of these discoveries led to the following…. ...
... • Was able to tell that DNA had an “X” like structure where the strands are twisted around each other • All of these discoveries led to the following…. ...
DNA Replication and recombination
... 1. Exam 2 next week: 10/17, 18, and 20. Same format as exam 1, a bit shorter. You will need a bluebook again. 2. Answers from exam 1 will be removed from lab when exam 2 answers go up. 3. Homework/ problem set 4 (15 pts) due next week in lab. ...
... 1. Exam 2 next week: 10/17, 18, and 20. Same format as exam 1, a bit shorter. You will need a bluebook again. 2. Answers from exam 1 will be removed from lab when exam 2 answers go up. 3. Homework/ problem set 4 (15 pts) due next week in lab. ...
Genetics Jeopardy - Maples Elementary School
... What is it called when a portion of the DNA is changed or missing? ...
... What is it called when a portion of the DNA is changed or missing? ...
5.DNA - Colorado State University
... All life shares the need for water and DNA (in every organism except some viruses that use RNA instead). DNA is copied to RNA, and RNA makes proteins that make organelles, and the organelles are found in cells that help the cell function properly, and cells build organisms. DNA’s code is like an alp ...
... All life shares the need for water and DNA (in every organism except some viruses that use RNA instead). DNA is copied to RNA, and RNA makes proteins that make organelles, and the organelles are found in cells that help the cell function properly, and cells build organisms. DNA’s code is like an alp ...
Renal transplant recipients
... M = Gender; 1 if male, 0 if female G = GSTT1 genotype; 1 if null, 0 if A ...
... M = Gender; 1 if male, 0 if female G = GSTT1 genotype; 1 if null, 0 if A ...
DNA Fingerprinting
... an X-ray film. Because restriction enzymes cut the DNA from different individuals into DNA fragments of different lengths (RFLPs), each individual has a unique pattern of banding or DNA fingerprint. ...
... an X-ray film. Because restriction enzymes cut the DNA from different individuals into DNA fragments of different lengths (RFLPs), each individual has a unique pattern of banding or DNA fingerprint. ...
Gel Electrophoresis
... Stages of DNA Profiling Stage ____: Fragments are _______ by size using process called __________________. DNA fragments ______ into wells and _______ current is applied along gel. A _________ material is added which combines with the DNA fragments to produce a _________ image. A ________ c ...
... Stages of DNA Profiling Stage ____: Fragments are _______ by size using process called __________________. DNA fragments ______ into wells and _______ current is applied along gel. A _________ material is added which combines with the DNA fragments to produce a _________ image. A ________ c ...
Presentation Slides - Genetics in Primary Care Institute
... • Limitations: why sequencing isn’t 100% – You only get data on what you sequence (=coding region) – If you only spell check one paragraph, you don’t know if there are errors in the rest of the text – You can only sequence what is there (no large deletions) – The spell-checker doesn’t tell you wheth ...
... • Limitations: why sequencing isn’t 100% – You only get data on what you sequence (=coding region) – If you only spell check one paragraph, you don’t know if there are errors in the rest of the text – You can only sequence what is there (no large deletions) – The spell-checker doesn’t tell you wheth ...
III. Biotechnology
... fragments and mutations are identified by an abnormal number of fragments ...
... fragments and mutations are identified by an abnormal number of fragments ...
DNA_Structure_2010
... Chromosomes – not just DNA! 1/3 DNA Histone proteins Other DNA binding proteins also a small amount of RNA ...
... Chromosomes – not just DNA! 1/3 DNA Histone proteins Other DNA binding proteins also a small amount of RNA ...
DNA Fingerprinting Lab
... One test used in forensic labs is DNA fingerprint. It is also called a DNA profile. Analysts use the DNA profile from potential suspects and compare it against DNA found at a crime scene. There’s DNA profiling for paternity tests. These days you can send a sample of DNA and find out your ancestry to ...
... One test used in forensic labs is DNA fingerprint. It is also called a DNA profile. Analysts use the DNA profile from potential suspects and compare it against DNA found at a crime scene. There’s DNA profiling for paternity tests. These days you can send a sample of DNA and find out your ancestry to ...
Working with Data Recombinant DNA
... Boyer pioneered the field of recombinant DNA technology when they demonstrated that biologically functional recombinant bacterial plasmids can be constructed in the laboratory. Specifically, the scientists used restriction enzymes to cut two E. coli plasmids containing a resistance gene for either k ...
... Boyer pioneered the field of recombinant DNA technology when they demonstrated that biologically functional recombinant bacterial plasmids can be constructed in the laboratory. Specifically, the scientists used restriction enzymes to cut two E. coli plasmids containing a resistance gene for either k ...
Example Quiz
... a. (2 pts) Tell why you did this (what was the value to your experiment in doing this step)? The goal was to remove the restriction enzyme from the DNA mixture. This was important as the next step was to ligate this DNA with the insert. If the EcoRI or HindIII was still present it would compete with ...
... a. (2 pts) Tell why you did this (what was the value to your experiment in doing this step)? The goal was to remove the restriction enzyme from the DNA mixture. This was important as the next step was to ligate this DNA with the insert. If the EcoRI or HindIII was still present it would compete with ...
Chapter 20 – DNA Technology - Fort Thomas Independent Schools
... 5. One feature of “engineered” plasmids that is helpful in the isolation and analysis of cloned DNA is: a) they can only handle DNA fragments of up to 120 kb b) that they are an integral part of all eukaryotic cells c) they contain no genetic material of their own so that the cloned fragment is trul ...
... 5. One feature of “engineered” plasmids that is helpful in the isolation and analysis of cloned DNA is: a) they can only handle DNA fragments of up to 120 kb b) that they are an integral part of all eukaryotic cells c) they contain no genetic material of their own so that the cloned fragment is trul ...
Microbiology: A Systems Approach, 2nd ed.
... – Will base-pair with a stretch of DNA with a complementary sequence if one exists in the test sample ...
... – Will base-pair with a stretch of DNA with a complementary sequence if one exists in the test sample ...
Repair of Damaged DNA
... phosphodiester backbone of DNA 2. Mismatch repair – replaces incorrect bases shortly after replication 3. Base-excision repair- cuts out damaged bases 4. Nucleotide-excision repair – removes largerscale distortions by excision ...
... phosphodiester backbone of DNA 2. Mismatch repair – replaces incorrect bases shortly after replication 3. Base-excision repair- cuts out damaged bases 4. Nucleotide-excision repair – removes largerscale distortions by excision ...
Genetic Profiling using Short Tandem Repeat Analysis
... fragments using PCR. The great advantage of PCR is its ability to amplify trace quantities of DNA. Then they separate the fragments from each other using capillary electrophoresis. Capillary electrophoresis has replaced gel electrophoresis with a series of capillaries ...
... fragments using PCR. The great advantage of PCR is its ability to amplify trace quantities of DNA. Then they separate the fragments from each other using capillary electrophoresis. Capillary electrophoresis has replaced gel electrophoresis with a series of capillaries ...
How can recombinant DNA be used?
... •Pesticide resistant plants- pesticides will kills pests (weeds, insects, etc.) but not plants •Oncomous – mouse with a gene for cancer (for research) •Pharming- inserting genes into livestock so they produce pharmaceutical products (like insulin in milk) •AquaAdvantage© Salmon – grow from egg to ma ...
... •Pesticide resistant plants- pesticides will kills pests (weeds, insects, etc.) but not plants •Oncomous – mouse with a gene for cancer (for research) •Pharming- inserting genes into livestock so they produce pharmaceutical products (like insulin in milk) •AquaAdvantage© Salmon – grow from egg to ma ...
Extracting DNA from Eukayotic Cells
... Background DNA is a nucleic acid found in the nucleus of cells that stores and transmits genetic information from one generation of an organism to the next by coding for the production of a cell’s proteins. Bacteria, fungi, plants, animals and all other living organisms on this planet contain nucl ...
... Background DNA is a nucleic acid found in the nucleus of cells that stores and transmits genetic information from one generation of an organism to the next by coding for the production of a cell’s proteins. Bacteria, fungi, plants, animals and all other living organisms on this planet contain nucl ...
Genetic Engineering - Biology Class With Mrs. Caskey
... • A specific gene has been added to the cats’ DNA which causes them to glow in the dark. • Normally, the gene is found in ...
... • A specific gene has been added to the cats’ DNA which causes them to glow in the dark. • Normally, the gene is found in ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.