Bio 102 Practice Problems The Double Helix
... 1. Experiments by Avery, McCarty and MacLeod were consistent with the hypothesis that DNA is the genetic material. However, at the time many scientists still didn't believe that DNA was the genetic material for a variety of logical reasons. Which one of the following was NOT cited as a reason to dou ...
... 1. Experiments by Avery, McCarty and MacLeod were consistent with the hypothesis that DNA is the genetic material. However, at the time many scientists still didn't believe that DNA was the genetic material for a variety of logical reasons. Which one of the following was NOT cited as a reason to dou ...
Genetic engineering
... Copying DNA It is relatively easy to extract DNA from cells and tissues. The extracted DNA can be cut into fragments of manageable size using restriction enzymes. These restriction fragments can then be separated according to size, using gel electrophoresis or another similar technique ...
... Copying DNA It is relatively easy to extract DNA from cells and tissues. The extracted DNA can be cut into fragments of manageable size using restriction enzymes. These restriction fragments can then be separated according to size, using gel electrophoresis or another similar technique ...
Case study I: DNA copy number changes
... Copy number variations (CNVs) Approx. 12% of the human genome consists of copy number variations ...
... Copy number variations (CNVs) Approx. 12% of the human genome consists of copy number variations ...
Biology 1710 - DFW Web Presence
... 50. The two strands of a DNA molecule are held together by: a. phosphodiester bonds. b. ionic bonds between the phosphates. c. hydrophobic interactions between the bases. d. hydrogen bonds. e. aminoacyl bonds. 51. Messenger RNAs are synthesized by: a. ribosomes. b. DNA polymerases. c. RNA polymerase ...
... 50. The two strands of a DNA molecule are held together by: a. phosphodiester bonds. b. ionic bonds between the phosphates. c. hydrophobic interactions between the bases. d. hydrogen bonds. e. aminoacyl bonds. 51. Messenger RNAs are synthesized by: a. ribosomes. b. DNA polymerases. c. RNA polymerase ...
Genetics - Georgia CTAE | Home
... information available to the cell Highly regulated by segment of DNA called a promoter ...
... information available to the cell Highly regulated by segment of DNA called a promoter ...
Genetics
... information available to the cell Highly regulated by segment of DNA called a promoter ...
... information available to the cell Highly regulated by segment of DNA called a promoter ...
DNA Profiling: How many CATS
... (CATCAT) while allele 2 has six repeats (CATCATCATCATCATCAT) that separate two restriction sites. A restriction site is a specific sequence of DNA that is recognized and cut by a restriction enzyme within the recognition sequence. When a restriction enzyme is used to cut the DNA of the restriction s ...
... (CATCAT) while allele 2 has six repeats (CATCATCATCATCATCAT) that separate two restriction sites. A restriction site is a specific sequence of DNA that is recognized and cut by a restriction enzyme within the recognition sequence. When a restriction enzyme is used to cut the DNA of the restriction s ...
2. You perform a Southern blot in which your probe should hybridize
... hands. It would NOT be because DNA from cells on your fingers hybridized to the probe. {Why? Even if the DNA in your cells was complementary to the probe, it is not available for hybridization as it is inside cells, and not denatured.} 3. Inadequate washing ...
... hands. It would NOT be because DNA from cells on your fingers hybridized to the probe. {Why? Even if the DNA in your cells was complementary to the probe, it is not available for hybridization as it is inside cells, and not denatured.} 3. Inadequate washing ...
DNA (Deoxyribonucleic Acid)
... Transcription/Translation Quiz Why is transcription necessary? Describe transcription. Why is translation necessary? Describe translation. What are the main differences between DNA and RNA. Using the chart on page 303, identify the amino acids coded for by these codons: UGGCAGUGC ...
... Transcription/Translation Quiz Why is transcription necessary? Describe transcription. Why is translation necessary? Describe translation. What are the main differences between DNA and RNA. Using the chart on page 303, identify the amino acids coded for by these codons: UGGCAGUGC ...
Name Ch 9 Homework- KEY 1. Cystic fibrosis is a recessive genetic
... replication where synthesis starts. DNA polymerase binds to the DNA strands at the origin of replication and via base pairing, begins to synthesize new daughter strands of DNA. The daughter strand grows in a 5’-3’ direction. Both strands of DNA are synthesizing new daughter strands at the same time. ...
... replication where synthesis starts. DNA polymerase binds to the DNA strands at the origin of replication and via base pairing, begins to synthesize new daughter strands of DNA. The daughter strand grows in a 5’-3’ direction. Both strands of DNA are synthesizing new daughter strands at the same time. ...
X-inactivation
... X-chromatin = Barr body (described by Barr and Bertram in 1949) inactivation begins in early embryonic development (probably at 1000- to 2000- cell stage) ...
... X-chromatin = Barr body (described by Barr and Bertram in 1949) inactivation begins in early embryonic development (probably at 1000- to 2000- cell stage) ...
Mitochondrial DNA Typing from Processed Fingerprints
... Fingerprints are routinely used in investigation to characterize individuals associated with forensic evidence. However, fingerprints are sometimes smeared or incomplete and cannot be interpreted. The use of mtDNA for the identification of the donator of these fingerprints would be valuable in foren ...
... Fingerprints are routinely used in investigation to characterize individuals associated with forensic evidence. However, fingerprints are sometimes smeared or incomplete and cannot be interpreted. The use of mtDNA for the identification of the donator of these fingerprints would be valuable in foren ...
Deoxyribonucleic Acid (DNA)
... History of DNA • ________ ________ identifies pattern of DNA using _____ diffraction (____). – Did not reveal _________ of DNA – Showed strands in DNA were _______ around each other. • Shape of _____. ...
... History of DNA • ________ ________ identifies pattern of DNA using _____ diffraction (____). – Did not reveal _________ of DNA – Showed strands in DNA were _______ around each other. • Shape of _____. ...
Study Questions
... smallest speck of blood, hair, etc., from your body behind because if you do, the DNA in this material can be amplified by __________, subjected to genetic analysis, and used to identify you as the perpetrator of the crime. A) ATP B) PCR C) blotting D) RFLP E) reverse transcriptase 20.16. The polyme ...
... smallest speck of blood, hair, etc., from your body behind because if you do, the DNA in this material can be amplified by __________, subjected to genetic analysis, and used to identify you as the perpetrator of the crime. A) ATP B) PCR C) blotting D) RFLP E) reverse transcriptase 20.16. The polyme ...
File
... The technique of chromosome painting is the result of scientific research. Scientists use chromosome painting to mark the locations of genes on human chromosomes with fluorescent tags. Its also possible to apply this technique to the chromosomes of many different species. Chromosome painting allows ...
... The technique of chromosome painting is the result of scientific research. Scientists use chromosome painting to mark the locations of genes on human chromosomes with fluorescent tags. Its also possible to apply this technique to the chromosomes of many different species. Chromosome painting allows ...
Determining the Structure of DNA
... entirely possible—and critical to curing human diseases. The discovery of DNA’s double-helix structure was a major blow to the vitalist approach and gave momentum to the reductionist field of molecular biology. ...
... entirely possible—and critical to curing human diseases. The discovery of DNA’s double-helix structure was a major blow to the vitalist approach and gave momentum to the reductionist field of molecular biology. ...
Ch 13 Genetic Engineering
... • DNA molecules are very long • Restriction enzymes – Enzymes that cuts DNA at a specific sequence of nucleotides ...
... • DNA molecules are very long • Restriction enzymes – Enzymes that cuts DNA at a specific sequence of nucleotides ...
Biotechnology
... DNA that contains genes from more than one organism or species • Plasmid – circular piece of genetic material found in bacteria that can replicate separately from the DNA of the main chromosome ...
... DNA that contains genes from more than one organism or species • Plasmid – circular piece of genetic material found in bacteria that can replicate separately from the DNA of the main chromosome ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.