Ch 13 student notes
... a. Their small size enables millions of organisms to be treated with radiation or chemicals at the same time b. Using this technique, scientists have been able to develop hundreds of beneficial strains, including bacteria that can clean up oil from oil spills 5. New varieties of plants have also bee ...
... a. Their small size enables millions of organisms to be treated with radiation or chemicals at the same time b. Using this technique, scientists have been able to develop hundreds of beneficial strains, including bacteria that can clean up oil from oil spills 5. New varieties of plants have also bee ...
Frontiers of Biotechnology
... Genetic screening can detect genetic disorders. – Determines risk __________________________________________________________________ – Used to _________________________________________________________________________ – Can ______________________________________ related to an increased risk of cancer ...
... Genetic screening can detect genetic disorders. – Determines risk __________________________________________________________________ – Used to _________________________________________________________________________ – Can ______________________________________ related to an increased risk of cancer ...
Agarose Gel Electrophoresis Description An electrophoresis
... recovery of DNA. Lower voltages, coupled with longer running times, provide optimum resolution, such as that required for Southern Blots or forensic applications. Pulsed-field electrophoresis can be used to separate very large DNA fragments. The most common stain is ethidium bromide, which intercala ...
... recovery of DNA. Lower voltages, coupled with longer running times, provide optimum resolution, such as that required for Southern Blots or forensic applications. Pulsed-field electrophoresis can be used to separate very large DNA fragments. The most common stain is ethidium bromide, which intercala ...
DNA replication
... phosphodiester backbone zigzags along the molecule. - Z-DNA is the least twisted (12bp/turn ) and has only one type of groove that may not bind to proteins that bind in the minor and major grooves of B form ,thus exert regulatory effects. ...
... phosphodiester backbone zigzags along the molecule. - Z-DNA is the least twisted (12bp/turn ) and has only one type of groove that may not bind to proteins that bind in the minor and major grooves of B form ,thus exert regulatory effects. ...
Next Generation Sequencing
... •. It is DNA sequencing technology which has revolutionized genomic research • Increasing the speed due to parallel analysis technology, and increase accuracy which decreased manpower and cost. • An entire human genome can be sequenced within a single day. In contrast, the previous Sanger sequencing ...
... •. It is DNA sequencing technology which has revolutionized genomic research • Increasing the speed due to parallel analysis technology, and increase accuracy which decreased manpower and cost. • An entire human genome can be sequenced within a single day. In contrast, the previous Sanger sequencing ...
File
... DNA replication occurs when _______________________________________________________________________ Replication produces _________ new strands of DNA that are _____________________________________________ 1st Step Base pairs ________________________________ & 2 ________________________________ ...
... DNA replication occurs when _______________________________________________________________________ Replication produces _________ new strands of DNA that are _____________________________________________ 1st Step Base pairs ________________________________ & 2 ________________________________ ...
SPIS TREŚCI
... although we have no need to be dogmatic about this. If it has occurred, it must have taken place when the human population was very small. The implications of this statement are quite profound. What is certain, however, is that major chromosomal variations that clearly exist between the human and th ...
... although we have no need to be dogmatic about this. If it has occurred, it must have taken place when the human population was very small. The implications of this statement are quite profound. What is certain, however, is that major chromosomal variations that clearly exist between the human and th ...
THE GENOME AND THE ORIGIN OF MAN
... although we have no need to be dogmatic about this. If it has occurred, it must have taken place when the human population was very small. The implications of this statement are quite profound. What is certain, however, is that major chromosomal variations that clearly exist between the human and th ...
... although we have no need to be dogmatic about this. If it has occurred, it must have taken place when the human population was very small. The implications of this statement are quite profound. What is certain, however, is that major chromosomal variations that clearly exist between the human and th ...
Food Safety and Beyond
... First, the target genetic material must be denatured-that is, the strands of its helix must be unwound and separated-by heating to 90-96°C. The second step is hybridization or annealing, in which the primers bind to their complementary bases on the now single-stranded DNA. The third is DNA synthesis ...
... First, the target genetic material must be denatured-that is, the strands of its helix must be unwound and separated-by heating to 90-96°C. The second step is hybridization or annealing, in which the primers bind to their complementary bases on the now single-stranded DNA. The third is DNA synthesis ...
Name: “Berry Full of DNA” DNA Extraction Lab Question: What
... carefully. Mash the strawberry with your fist and fingers for 2 min. 2. Add the 10 ml detergent solution to the bag. Press the air out carefully and seal the bag. 3. Mash the bagged strawberry for 1 min. 4. Set up your filtration apparatus as shown below. If a test tube rack is available, place the ...
... carefully. Mash the strawberry with your fist and fingers for 2 min. 2. Add the 10 ml detergent solution to the bag. Press the air out carefully and seal the bag. 3. Mash the bagged strawberry for 1 min. 4. Set up your filtration apparatus as shown below. If a test tube rack is available, place the ...
Chapter 13-DNA Technology
... • Introducing (through a vector) a HEALTHLY GENE into a cell OR by CORRECTING a gene defect in a cell’s genome. EX: Nasal sprays (w/ NORMAL cystic fibrosis gene) are INHALED into nose and lungs, where there are cells affected by diseased c.f. genes. ...
... • Introducing (through a vector) a HEALTHLY GENE into a cell OR by CORRECTING a gene defect in a cell’s genome. EX: Nasal sprays (w/ NORMAL cystic fibrosis gene) are INHALED into nose and lungs, where there are cells affected by diseased c.f. genes. ...
Chromosomal Genetics and Pathology (Dr
... unequal recombination b/w OR clusters on chrom. 8 (short arm) results in three recurrent chromosomal rearrangements: inverted duplication (distinct phenotype), supranumery chromosome (minor anomalies), 8p23 interstitial deletion (heart defect) these recombination events are associated with a mat ...
... unequal recombination b/w OR clusters on chrom. 8 (short arm) results in three recurrent chromosomal rearrangements: inverted duplication (distinct phenotype), supranumery chromosome (minor anomalies), 8p23 interstitial deletion (heart defect) these recombination events are associated with a mat ...
Name - LEMA
... Manipulating DNA Since the 1970s, techniques have been developed that allow scientists to cut, separate, and replicate DNA base-by-base. Using these tools, scientists can read the base sequences in DNA from any cell. Restriction enzymes cut DNA into smaller pieces, called restriction fragments, whi ...
... Manipulating DNA Since the 1970s, techniques have been developed that allow scientists to cut, separate, and replicate DNA base-by-base. Using these tools, scientists can read the base sequences in DNA from any cell. Restriction enzymes cut DNA into smaller pieces, called restriction fragments, whi ...
GEL ELECTROPHORESIS VIRTUAL LAB
... Directions: Log on the computer and go to http://learn.genetics.utah.edu/content/labs/gel/ For each section read the question first and then read through the information on the website. As you go through the virtual lab, be sure to read all directions, follow all prompts given to you, and answer all ...
... Directions: Log on the computer and go to http://learn.genetics.utah.edu/content/labs/gel/ For each section read the question first and then read through the information on the website. As you go through the virtual lab, be sure to read all directions, follow all prompts given to you, and answer all ...
Gene Linkage
... Limitations of selective breeding and mutations: – Selective breeding requires traits already exists in a population – we can not make new traits. – Mutations are unpredictable and will not create the exact traits that we want. (most mutations are harmful to the organism) Scientists are learning how ...
... Limitations of selective breeding and mutations: – Selective breeding requires traits already exists in a population – we can not make new traits. – Mutations are unpredictable and will not create the exact traits that we want. (most mutations are harmful to the organism) Scientists are learning how ...
The protein that assesses distances
... “What we observed in our calculations is that the longer the DNA segment between one nucleosome and the next the shorter the time it takes the motor to bind to it”. In fact the strands immersed in fluid tend to fluctuate randomly and the magnitude and speed of their movement depend on the length ...
... “What we observed in our calculations is that the longer the DNA segment between one nucleosome and the next the shorter the time it takes the motor to bind to it”. In fact the strands immersed in fluid tend to fluctuate randomly and the magnitude and speed of their movement depend on the length ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.