Ch. 13 – Biotechnology
... § restriction endonucleases § discovered in 1960s § evolved in bacteria to cut up foreign DNA § “restrict” action of attacking organisms (viruses and other bacteria) § How do bacteria protect their own DNA? § Methylation ...
... § restriction endonucleases § discovered in 1960s § evolved in bacteria to cut up foreign DNA § “restrict” action of attacking organisms (viruses and other bacteria) § How do bacteria protect their own DNA? § Methylation ...
SG 17,18,19
... What are endogenous and exogenous forces, what effect on DNA. Discuss how the structure of DNA was determined. Describe basic structure, types of DNA. Discuss supercoiling and it’s role in DNA replication. Define chromosome. Describe chomosomes in prokaryotes versus eukaryotes. Compare Prokaryotic g ...
... What are endogenous and exogenous forces, what effect on DNA. Discuss how the structure of DNA was determined. Describe basic structure, types of DNA. Discuss supercoiling and it’s role in DNA replication. Define chromosome. Describe chomosomes in prokaryotes versus eukaryotes. Compare Prokaryotic g ...
AP genetic technology
... • Hamilton Smith was studying how Haemophilus influenzae defend themselves from bacteriophage attack – Discovered bacteria have an enzyme that chops up viral DNA • Restriction enzymes cut DNA at a specific sequence • Number of cuts made in DNA will depend on number of times the “target” sequence occ ...
... • Hamilton Smith was studying how Haemophilus influenzae defend themselves from bacteriophage attack – Discovered bacteria have an enzyme that chops up viral DNA • Restriction enzymes cut DNA at a specific sequence • Number of cuts made in DNA will depend on number of times the “target” sequence occ ...
ANSWER KEY FOR PROBLEM SET #1
... Ultrasound: The fetus can be “seen” in the mother’s womb. This procedure is used to detect multiples, physical defects and the position of the developing fetus and placenta. The procedure has no specific time limitations. Endoamnioscopy: A technique permitting direct visualization of the developing ...
... Ultrasound: The fetus can be “seen” in the mother’s womb. This procedure is used to detect multiples, physical defects and the position of the developing fetus and placenta. The procedure has no specific time limitations. Endoamnioscopy: A technique permitting direct visualization of the developing ...
Bio Ch. 12-1 DNA and RNA notes
... The Hershey-Chase experiment was based on the fact that a) DNA has both sulfur and phosphorus in its ...
... The Hershey-Chase experiment was based on the fact that a) DNA has both sulfur and phosphorus in its ...
Make a DNA Model - Flinn Scientific
... is bonded with a nitrogenous base. Four types of nitrogen bases are present in DNA—adenine, cytosine, thymine, and guanine. The bases on opposite strands in the double-stranded structure of DNA are complementary, meaning that adenine only pairs with thymine and cytosine only pairs with guanine in DN ...
... is bonded with a nitrogenous base. Four types of nitrogen bases are present in DNA—adenine, cytosine, thymine, and guanine. The bases on opposite strands in the double-stranded structure of DNA are complementary, meaning that adenine only pairs with thymine and cytosine only pairs with guanine in DN ...
Genetics HARDCOPY - New Hartford Central Schools
... How is a karyotype made? Amniocentesis - a test in which a small amount of fluid ...
... How is a karyotype made? Amniocentesis - a test in which a small amount of fluid ...
Chapter 13 Genetics and Biotechnology
... sequencing the DNA the next step is identifying genes. Originally thought that humans had 100,000 genes because we have 100,000 proteins (one gene-one protein) Now know that we have 20,000-25,000 genes. Complicated analysis that uses information from other genomes DNA sequences along with comp ...
... sequencing the DNA the next step is identifying genes. Originally thought that humans had 100,000 genes because we have 100,000 proteins (one gene-one protein) Now know that we have 20,000-25,000 genes. Complicated analysis that uses information from other genomes DNA sequences along with comp ...
clicker review
... like to compare it to samples from living birds. Which of the following would be most useful for increasing the amount of DNA for testing? A RFLP analysis B Polymerase chain reaction (PCR) C electroporation D gel electrophoresis E restriction fragment ligation 21. In recombinant gene technology, a v ...
... like to compare it to samples from living birds. Which of the following would be most useful for increasing the amount of DNA for testing? A RFLP analysis B Polymerase chain reaction (PCR) C electroporation D gel electrophoresis E restriction fragment ligation 21. In recombinant gene technology, a v ...
The amount of DNA, # of genes and DNA per gene in various
... The first proof was provided In 1961 by measuring the ratio of different dinucleotides in DNA. The concentration of 5’AG3’ was equal to 5’CT3’ (as expected from an antiparallel orientation) and not equal to 5’TC3’ (as expected from a a parallel orientation). DNA sequencing in 1970s confirmed this co ...
... The first proof was provided In 1961 by measuring the ratio of different dinucleotides in DNA. The concentration of 5’AG3’ was equal to 5’CT3’ (as expected from an antiparallel orientation) and not equal to 5’TC3’ (as expected from a a parallel orientation). DNA sequencing in 1970s confirmed this co ...
Welcome to the Chapter 12 Test!
... • Discuss each answer before writing! • During the discussion, decide which concepts from the notes to include in your answer. • Remember to underline each concept. • If you can’t whisper, you will work alone. ...
... • Discuss each answer before writing! • During the discussion, decide which concepts from the notes to include in your answer. • Remember to underline each concept. • If you can’t whisper, you will work alone. ...
Sunflower DNA extraction for RFLP and PCR
... Sunflower is a recalcitrant plant to obtain qualified DNA for RFLP analysis because of its high polyphenols, polysaccharides and tannins. SDS-KAc method and common CTAB method (precipitate DNA directly from DNA-CTAB buffer using isopropanol) can not produce highly purified DNA because DNA co-precipi ...
... Sunflower is a recalcitrant plant to obtain qualified DNA for RFLP analysis because of its high polyphenols, polysaccharides and tannins. SDS-KAc method and common CTAB method (precipitate DNA directly from DNA-CTAB buffer using isopropanol) can not produce highly purified DNA because DNA co-precipi ...
Pedigree
... are family trees that explain your genetic history. Pedigrees are used to find out the probability of a child having a disorder in a particular family. To begin to interpret a pedigree, determine if the disease or condition is autosomal or X-linked and dominant or recessive. ...
... are family trees that explain your genetic history. Pedigrees are used to find out the probability of a child having a disorder in a particular family. To begin to interpret a pedigree, determine if the disease or condition is autosomal or X-linked and dominant or recessive. ...
Biotechnology toolkit part 1 File
... The protein coding region- contains the nucleotide triplet codes which code for specific amino acids and the order they are arranged in. This is a universal code so the same protein can in theory be made by any organism. ...
... The protein coding region- contains the nucleotide triplet codes which code for specific amino acids and the order they are arranged in. This is a universal code so the same protein can in theory be made by any organism. ...
Pedigree
... are family trees that explain your genetic history. Pedigrees are used to find out the probability of a child having a disorder in a particular family. To begin to interpret a pedigree, determine if the disease or condition is autosomal or X-linked and dominant or recessive. ...
... are family trees that explain your genetic history. Pedigrees are used to find out the probability of a child having a disorder in a particular family. To begin to interpret a pedigree, determine if the disease or condition is autosomal or X-linked and dominant or recessive. ...
Study Guide
... replication forks in a single cell.” Explain how Table 1 provides supporting evidence for these statements. (“Doubling time” is the time it takes for a cell to divide into two daughter cells. The shorter the doubling time, the faster the growth rate.) Why were three different supplements (succinate, ...
... replication forks in a single cell.” Explain how Table 1 provides supporting evidence for these statements. (“Doubling time” is the time it takes for a cell to divide into two daughter cells. The shorter the doubling time, the faster the growth rate.) Why were three different supplements (succinate, ...
Fernanda Appleton Biology 1615 Research Paper:” The Oxytricha
... and rarely conjugates the result is meiotic recombination. Conjugation in the laboratory is induced by starvation as long as cells of compatible mating types are available, but the Scientists do not know if this also can occur in a natural environment. Oxytricha trifallax strain JRB310 was cultured ...
... and rarely conjugates the result is meiotic recombination. Conjugation in the laboratory is induced by starvation as long as cells of compatible mating types are available, but the Scientists do not know if this also can occur in a natural environment. Oxytricha trifallax strain JRB310 was cultured ...
No Slide Title
... bacteria • Lambda phage and P1 phage can carry large fragments of DNA – 20 kb for lambda – 70 to 300 kb for P1 ...
... bacteria • Lambda phage and P1 phage can carry large fragments of DNA – 20 kb for lambda – 70 to 300 kb for P1 ...
SPMS Unit 3.1 DNA Profiling File
... a. What do they have in common? b. Describe how they differ? c. How are they each used in forensics? Elaborate on STRs used in DNA analysis. a. STR is an abbreviation for which words? b. Where do you find STRs? c. How many bases are usually found within an STR? d. List several examples of STRs. e. I ...
... a. What do they have in common? b. Describe how they differ? c. How are they each used in forensics? Elaborate on STRs used in DNA analysis. a. STR is an abbreviation for which words? b. Where do you find STRs? c. How many bases are usually found within an STR? d. List several examples of STRs. e. I ...
Chapter 16 Recombination DNA and Genetic Engineering
... set is identified by laser beam. – The computer program in the machine assembles the information from all the nucleotides in the sample to reveal the entire DNA sequence ...
... set is identified by laser beam. – The computer program in the machine assembles the information from all the nucleotides in the sample to reveal the entire DNA sequence ...
Chromosome Chromo
... Cytogenetics = The study of chromosome number, structure, function, and behavior in relation to gene inheritance, organization and expression ...
... Cytogenetics = The study of chromosome number, structure, function, and behavior in relation to gene inheritance, organization and expression ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.