Sterile, 24-well tissue culture plates are filled with melted minimal ... 1.0 ml per well using a repeating syringe. After the...
... Complementation studies including mts(MN9), cpc-1 (CD86 and j5) and mts(MN1) were performed as already described for mts MNl) (Koch and Bartheless, 1987). The mutants were recessive to their respective wild type alleles, but complementation of the amino acid analogue sensitive phenotype was not obs ...
... Complementation studies including mts(MN9), cpc-1 (CD86 and j5) and mts(MN1) were performed as already described for mts MNl) (Koch and Bartheless, 1987). The mutants were recessive to their respective wild type alleles, but complementation of the amino acid analogue sensitive phenotype was not obs ...
How to determine whether a strain will undergo senescence. Background
... accumulate and slow or terminate growth even in normal growing, nonsenescent cultures. Occasional outcrossing can purge the genome of the accumulated defective genes, but in the absence of genetic recombination, deleterious mutations are expected ultimately to result in death of a serially propogate ...
... accumulate and slow or terminate growth even in normal growing, nonsenescent cultures. Occasional outcrossing can purge the genome of the accumulated defective genes, but in the absence of genetic recombination, deleterious mutations are expected ultimately to result in death of a serially propogate ...
Disrupting antibiotic resistance propagation by inhibiting
... 1,2-bis(dimethoxyphosphoryl)benzene (PBENP) (Fig. 3). Results from this screen reveal that effective inhibitors have two phosphonate moieties separated by three or fewer atoms and have no additional negative charge at pH 7.4. Four clinically approved bisphosphonates were tested; these drugs are used ...
... 1,2-bis(dimethoxyphosphoryl)benzene (PBENP) (Fig. 3). Results from this screen reveal that effective inhibitors have two phosphonate moieties separated by three or fewer atoms and have no additional negative charge at pH 7.4. Four clinically approved bisphosphonates were tested; these drugs are used ...
Problem Set V - Biology 2970
... In general, individuals with Down's syndrome are trisomic for a small acrocentric chromosome that is designated chromosome 21. Such trisomic individuals have 47 chromosomes rather than the normal 46. Down's syndrome patients that have 46 chromosomes are occasionally found, however. Almost always in ...
... In general, individuals with Down's syndrome are trisomic for a small acrocentric chromosome that is designated chromosome 21. Such trisomic individuals have 47 chromosomes rather than the normal 46. Down's syndrome patients that have 46 chromosomes are occasionally found, however. Almost always in ...
Cutting Edge: DNA Polymerases and Are Dispensable for Ig Gene
... transcription in lymphoid tissues, in particular B cells from tonsils, but also, and obviously not in favor of an Ig mutase, in thymus; 3) its rather high level of infidelity during DNA synthesis, which classified it as one of the most error-prone polymerases described so far (13). However, the last ...
... transcription in lymphoid tissues, in particular B cells from tonsils, but also, and obviously not in favor of an Ig mutase, in thymus; 3) its rather high level of infidelity during DNA synthesis, which classified it as one of the most error-prone polymerases described so far (13). However, the last ...
Caspary T, Anderson KV. Dev Dyn. 2006 Sep;235(9):2412-23. Uncovering the uncharacterized and unexpected: unbiased phenotype-driven screens in the mouse. (Review)
... the past five years from a potential approach to a practical tool. This change has been driven by the relative ease of identifying causative mutations now that the complete genome sequence is available. These unbiased screens make it possible to identify genes, gene functions and processes that are ...
... the past five years from a potential approach to a practical tool. This change has been driven by the relative ease of identifying causative mutations now that the complete genome sequence is available. These unbiased screens make it possible to identify genes, gene functions and processes that are ...
Modifiers of epigenetic reprogramming show paternal effects in the mouse
... the Avy allele. As reported previously, in offspring that inherit the Smarca5MommeD4 allele, we observed a shift in penetrance at Avy in a manner consistent with its role as an enhancer of variegation at the transgene locus. In other words, Smarca5MommeD4/+ offspring are less likely to be yellow tha ...
... the Avy allele. As reported previously, in offspring that inherit the Smarca5MommeD4 allele, we observed a shift in penetrance at Avy in a manner consistent with its role as an enhancer of variegation at the transgene locus. In other words, Smarca5MommeD4/+ offspring are less likely to be yellow tha ...
C1. Duplications and deficiencies involve a change in the total
... conditions. When polyploid plants have an odd number of sets, they are typically seedless. This can be a desirable trait for certain fruit-producing crops such as bananas. C30. The turtles are two distinct species that appear phenotypically identical. The turtles with 48 chromosomes are polyploid re ...
... conditions. When polyploid plants have an odd number of sets, they are typically seedless. This can be a desirable trait for certain fruit-producing crops such as bananas. C30. The turtles are two distinct species that appear phenotypically identical. The turtles with 48 chromosomes are polyploid re ...
Document
... conditions. When polyploid plants have an odd number of sets, they are typically seedless. This can be a desirable trait for certain fruit-producing crops such as bananas. C30. The turtles are two distinct species that appear phenotypically identical. The turtles with 48 chromosomes are polyploid re ...
... conditions. When polyploid plants have an odd number of sets, they are typically seedless. This can be a desirable trait for certain fruit-producing crops such as bananas. C30. The turtles are two distinct species that appear phenotypically identical. The turtles with 48 chromosomes are polyploid re ...
University of Groningen Soft tissue sarcoma at the turn of the
... medulloblastoma [29]). In liposarcomas, there are indications that amplification of genes located at 1q21-24, often with concomitant gain in 12q14-21, plays a significant role in development and progression [30]. Furthermore, the long arm of chromosome 1 is a region of particular interest with regar ...
... medulloblastoma [29]). In liposarcomas, there are indications that amplification of genes located at 1q21-24, often with concomitant gain in 12q14-21, plays a significant role in development and progression [30]. Furthermore, the long arm of chromosome 1 is a region of particular interest with regar ...
Cepheid Xpert Carba-R
... PCC=FAIL: all or one probe check result failed possibly because the reaction tube was filled improperly or a probe integrity problem was detected; if the probe check passed, the error is caused by a system component failure Presence/absence of the 5 targets cannot be determined - repeat test. In ...
... PCC=FAIL: all or one probe check result failed possibly because the reaction tube was filled improperly or a probe integrity problem was detected; if the probe check passed, the error is caused by a system component failure Presence/absence of the 5 targets cannot be determined - repeat test. In ...
SOME EVIDENCE CONCERNING THE "MUTATIONAL
... Of these, deaths due to recessive lethals are only likely to occur with an appreciable frequency when the parents are related, as for instance in an inbred strain. However, it has usually been supposed that the greater part of embryonic death, even in an inbred strain, is due to the fourth cause, i. ...
... Of these, deaths due to recessive lethals are only likely to occur with an appreciable frequency when the parents are related, as for instance in an inbred strain. However, it has usually been supposed that the greater part of embryonic death, even in an inbred strain, is due to the fourth cause, i. ...
Influence of the environment and probes on rapid DNA sequencing
... In their pioneering work Kasianowicz et al. demonstrated that DNA can be pulled through a biological nanopore roughly the size of the DNA itself. [4] The translocation of the DNA can be detected by measuring a blockade current when ions are partially prevented from entering the pore. More recent exp ...
... In their pioneering work Kasianowicz et al. demonstrated that DNA can be pulled through a biological nanopore roughly the size of the DNA itself. [4] The translocation of the DNA can be detected by measuring a blockade current when ions are partially prevented from entering the pore. More recent exp ...
How many lethal alleles? - University of Edinburgh
... population (i.e. large, random mating, even sex ratio, non-overlapping generations) that is affected by drift and selection to the same extent as the population under consideration. Gynogenesis: A form of female parthenogenesis in which the embryo only contains maternal chromosomes, owing to the spe ...
... population (i.e. large, random mating, even sex ratio, non-overlapping generations) that is affected by drift and selection to the same extent as the population under consideration. Gynogenesis: A form of female parthenogenesis in which the embryo only contains maternal chromosomes, owing to the spe ...
HALLBERG
... ribosome accumulation occur coordinately in exponentially growing cells, and that these rates are drastically reduced during starvation. Upon refeeding, the rate of ribosomal protein synthesis per cell increases SO-fold before any accumulation of new ribosomes is seen. In addition, different ribosom ...
... ribosome accumulation occur coordinately in exponentially growing cells, and that these rates are drastically reduced during starvation. Upon refeeding, the rate of ribosomal protein synthesis per cell increases SO-fold before any accumulation of new ribosomes is seen. In addition, different ribosom ...
Chapter 12
... Eukaryotic genes are frequently interrupted by non-coding introns, which may be much larger than the protein-coding regions ...
... Eukaryotic genes are frequently interrupted by non-coding introns, which may be much larger than the protein-coding regions ...
PKB - Friedrich Miescher Institute for Biomedical Research
... indicates wild-type sequence, "MUT" indicates that the tumors contained a mutation of the PIK3CA gene, "LOH" refers to cases wherein the wild-type allele was lost and only the mutant allele remained, and "del" indicates a deletion of the indicated nucleotide(s). Mutations in red are likely to be act ...
... indicates wild-type sequence, "MUT" indicates that the tumors contained a mutation of the PIK3CA gene, "LOH" refers to cases wherein the wild-type allele was lost and only the mutant allele remained, and "del" indicates a deletion of the indicated nucleotide(s). Mutations in red are likely to be act ...
Biology Ch. 13
... These fragments were combined with vectors to create recombinant DNA, cloned to make many copies, and sequenced using automated sequencing machines. Computers analyzed the overlapping regions to generate one continuous sequence. ...
... These fragments were combined with vectors to create recombinant DNA, cloned to make many copies, and sequenced using automated sequencing machines. Computers analyzed the overlapping regions to generate one continuous sequence. ...
NEW EVIDENCE FOR THE HOMOLOGY OF THE SHORT
... similarity alone is, of course, insufficient to draw conclusions about homology. I n this case, as for Cell, the important criterion must be the positions of the loci of the mutations under investigation to those of other mutations which are already known and typical for the given chromosome or chro ...
... similarity alone is, of course, insufficient to draw conclusions about homology. I n this case, as for Cell, the important criterion must be the positions of the loci of the mutations under investigation to those of other mutations which are already known and typical for the given chromosome or chro ...
E.coli
... Sometimes we have at least part of the gene sequence and can fish for it Other times we know the amino acid sequence and can work backward, but with degeneracy (64 codons, 20 aa’s) Typically use at least 17mers to guarantee that the don’t get random association Probes derived from a different specie ...
... Sometimes we have at least part of the gene sequence and can fish for it Other times we know the amino acid sequence and can work backward, but with degeneracy (64 codons, 20 aa’s) Typically use at least 17mers to guarantee that the don’t get random association Probes derived from a different specie ...
Jeopardy Template
... In a test cross, the unknown individual is usually crossed with a known individual of this genotype: ...
... In a test cross, the unknown individual is usually crossed with a known individual of this genotype: ...
Primer on Molecular Genetics
... If unwound and tied together, the strands of DNA would stretch more than 5 feet but would be only 50 trillionths of an inch wide. For each organism, the components of these slender threads encode all the information necessary for building and maintaining life, from simple bacteria to remarkably comp ...
... If unwound and tied together, the strands of DNA would stretch more than 5 feet but would be only 50 trillionths of an inch wide. For each organism, the components of these slender threads encode all the information necessary for building and maintaining life, from simple bacteria to remarkably comp ...
Chromosome Number
... Genes carried on the female sex chromosome (X) are said to be X-linked (or sex-linked) X-linked genes have a different pattern of inheritance than autosomal genes have The Y chromosome is blank for these genes Recessive alleles on X chromosome: - Follow familiar dominant/recessive rules in females ...
... Genes carried on the female sex chromosome (X) are said to be X-linked (or sex-linked) X-linked genes have a different pattern of inheritance than autosomal genes have The Y chromosome is blank for these genes Recessive alleles on X chromosome: - Follow familiar dominant/recessive rules in females ...
Mutagen
In genetics, a mutagen is a physical or chemical agent that changes the genetic material, usually DNA, of an organism and thus increases the frequency of mutations above the natural background level. As many mutations can cause cancer, mutagens are therefore also likely to be carcinogens. Not all mutations are caused by mutagens: so-called ""spontaneous mutations"" occur due to spontaneous hydrolysis, errors in DNA replication, repair and recombination.