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beyond binding: molecular and cell biological approaches to
... to insert an antigenic epitope into the GPCR, which can facilitate detection and purification by standard immunochemical procedures using well-characterized antibodies (see ref. 25 for review). This approach obviates the sometimes laborious path of generating antibodies that recognize the native rec ...
... to insert an antigenic epitope into the GPCR, which can facilitate detection and purification by standard immunochemical procedures using well-characterized antibodies (see ref. 25 for review). This approach obviates the sometimes laborious path of generating antibodies that recognize the native rec ...
Serotonin 5-HT Receptors: Chemical Neuronatomy in the
... pivotal in the discovery of 5-HT2C receptors, which were first identified by autoradiography after labeling of rat brain sections with the 5-HT2A and dopamine D2 receptor ligand mesulergine. This was followed by a thorough pharmacological characterization performed in pig brain choroid plexus, and t ...
... pivotal in the discovery of 5-HT2C receptors, which were first identified by autoradiography after labeling of rat brain sections with the 5-HT2A and dopamine D2 receptor ligand mesulergine. This was followed by a thorough pharmacological characterization performed in pig brain choroid plexus, and t ...
The Physiology of the Distal Tubules and Collecting Ducts
... by the osmotic gradient generated by countercurrent concentrating mechanism in the loop of Henle. • Na+ reabsorption along the collecting duct dilutes luminal fluid and generates an osmotic gradient favorable to water reabsorption. • Water reabsorption along the collecting duct is controlled chiefly ...
... by the osmotic gradient generated by countercurrent concentrating mechanism in the loop of Henle. • Na+ reabsorption along the collecting duct dilutes luminal fluid and generates an osmotic gradient favorable to water reabsorption. • Water reabsorption along the collecting duct is controlled chiefly ...
Amino acid metabolism 2 - LSU School of Medicine
... •MAO inhibitors and methamphetamine block catecholamine degradation, allowing their accumulation in the presynaptic neuron and subsequent leakage into circulation, providing an antidepressant action. ...
... •MAO inhibitors and methamphetamine block catecholamine degradation, allowing their accumulation in the presynaptic neuron and subsequent leakage into circulation, providing an antidepressant action. ...
Novel Antipeptide Antibodies to the Human Glucocorticoid Receptor
... physiological effects of steroid hormones appear to be mediated via selective interaction of steroid-receptor complexes with enhancer-like elements that are usually found in the promoter regions of steroid-responsive genes (1, 2). During the past few years, the cloning and functional analysis of man ...
... physiological effects of steroid hormones appear to be mediated via selective interaction of steroid-receptor complexes with enhancer-like elements that are usually found in the promoter regions of steroid-responsive genes (1, 2). During the past few years, the cloning and functional analysis of man ...
Transcription factors
... – only 1st finger binds to DNA – second finger is responsible for protein:protein interactions – spacing between fingers can vary quite a bit finger 1 contains a regions that determines target specificity - P-box – CGSCKA - AGAACA – CEGCKG - AGTTCA – these can be swapped and change specificity of th ...
... – only 1st finger binds to DNA – second finger is responsible for protein:protein interactions – spacing between fingers can vary quite a bit finger 1 contains a regions that determines target specificity - P-box – CGSCKA - AGAACA – CEGCKG - AGTTCA – these can be swapped and change specificity of th ...
receptors
... Can Be Received by Cells • Signaling molecules are often classified based on the distance between the site of production and the target – Endocrine signals are produced far from the target tissues, which they reach via the circulatory system – Paracrine signals are diffusible and act over a ...
... Can Be Received by Cells • Signaling molecules are often classified based on the distance between the site of production and the target – Endocrine signals are produced far from the target tissues, which they reach via the circulatory system – Paracrine signals are diffusible and act over a ...
The glutamate receptor of the Qp-type activates protein kinase C
... induced translocation reached its maximal value 5 min after phorbol 12,13-dibutyrate addition and remained constant thereafter [19]. A rapid and transient effect similar to the one shown in Fig. 1 has also been observed after activation of other transmembrane receptors as for example the muscarinic ...
... induced translocation reached its maximal value 5 min after phorbol 12,13-dibutyrate addition and remained constant thereafter [19]. A rapid and transient effect similar to the one shown in Fig. 1 has also been observed after activation of other transmembrane receptors as for example the muscarinic ...
Satisfying the Immense Energy Demands of the Body, and the
... and Yoshida, 2001; Alberts et al., 2004). Interestingly, this rotary enzyme can operate in reverse (i.e. ATP hydrolysis). The cell carefully controls its supply of ATP, not wanting too little or too much of it. If there is too much ATP, simple Brownian motion of the pool of ATP and ADP will lead to ...
... and Yoshida, 2001; Alberts et al., 2004). Interestingly, this rotary enzyme can operate in reverse (i.e. ATP hydrolysis). The cell carefully controls its supply of ATP, not wanting too little or too much of it. If there is too much ATP, simple Brownian motion of the pool of ATP and ADP will lead to ...
Glucose-dependent, CAMP-mediated ATP efflux from
... In most tissues and organs, extracellular adenine nucleotides have diverse physiological effects that are principally mediated by the P2 purinergic receptors (Dubyak & El-Moatassim, 1993; Chen et al., 1995; Lewis et al., 1995). In some cases, such as the stimulation of platelet aggregation and the m ...
... In most tissues and organs, extracellular adenine nucleotides have diverse physiological effects that are principally mediated by the P2 purinergic receptors (Dubyak & El-Moatassim, 1993; Chen et al., 1995; Lewis et al., 1995). In some cases, such as the stimulation of platelet aggregation and the m ...
Differential Internalization of the Prostaglandin F2α Receptor
... albumin, Opti-MEM, hygromycin B, geneticin, and gentamicin reagent solutions were obtained from Invitrogen (Carlsbad, CA). Gö 6976 and phorbol 12-myristyl 13-acetate (PMA) were purchased from Calbiochem (San Diego, CA). Clathrin heavy-chain antibodies and anti-phosphotyrosine (PY20) antibodies were ...
... albumin, Opti-MEM, hygromycin B, geneticin, and gentamicin reagent solutions were obtained from Invitrogen (Carlsbad, CA). Gö 6976 and phorbol 12-myristyl 13-acetate (PMA) were purchased from Calbiochem (San Diego, CA). Clathrin heavy-chain antibodies and anti-phosphotyrosine (PY20) antibodies were ...
From Hans Selye`s discovery of biological stress to the
... mune, and visceral efferent limbs of the stress response. For instance brain CRF activates the sympathetic nervous system inducing the systemic release of catecholamines involved in the ”fight or flight” response. The locus coeruleus is also activated by CRF and its noradrenergic projections to the ...
... mune, and visceral efferent limbs of the stress response. For instance brain CRF activates the sympathetic nervous system inducing the systemic release of catecholamines involved in the ”fight or flight” response. The locus coeruleus is also activated by CRF and its noradrenergic projections to the ...
Expression and Purification of Functional Ligand
... This lack of progress is largely due to the difficulty in obtaining large amounts of purified receptor proteins suitable for biochemical or structural studies. For example, receptor proteins outside the hydrophobic environment of their native lipid membrane may not maintain their normal tertiary str ...
... This lack of progress is largely due to the difficulty in obtaining large amounts of purified receptor proteins suitable for biochemical or structural studies. For example, receptor proteins outside the hydrophobic environment of their native lipid membrane may not maintain their normal tertiary str ...
9700/04 - StudyGuide.PK
... (a) Describe the importance of ATP in cells, giving two examples of processes in which it is used. ...
... (a) Describe the importance of ATP in cells, giving two examples of processes in which it is used. ...
Purinergic signalling
Purinergic signalling (or signaling: see American and British English differences) is a form of extracellular signalling mediated by purine nucleotides and nucleosides such as adenosine and ATP. It involves the activation of purinergic receptors in the cell and/or in nearby cells, thereby regulating cellular functions.The purinergic signalling complex of a cell is sometimes referred to as the “purinome”.