Direct In Vitro Effect of a Sulfonylurea to Increase Human Fibroblast
... et al. (17) suggest a possible mechanism. Thus, it has been proposed that the concentration of cell surface insulin receptors reflects a dynamic state of equilibrium between receptor degradation or intemalization and receptor synthesis and reinsertion, and recent work from our own laboratory using c ...
... et al. (17) suggest a possible mechanism. Thus, it has been proposed that the concentration of cell surface insulin receptors reflects a dynamic state of equilibrium between receptor degradation or intemalization and receptor synthesis and reinsertion, and recent work from our own laboratory using c ...
The cyanide hydratase from Neurospora crassa forms a helix
... was calculated as the sum of the individual power spectra of the images (Wang et al. 2006). Three-dimensional reconstruction Three-dimensional reconstruction was achieved using the iterative helical real space reconstruction (IHRSR; Egelman 2000). Starting models were either a 12-nm-diameter feature ...
... was calculated as the sum of the individual power spectra of the images (Wang et al. 2006). Three-dimensional reconstruction Three-dimensional reconstruction was achieved using the iterative helical real space reconstruction (IHRSR; Egelman 2000). Starting models were either a 12-nm-diameter feature ...
Gene Regulatory Potential of 1α,25-Dihydroxyvitamin D3
... vitamin D receptor (VDR) via combined contact with the retinoid X receptor (RXR), coactivator proteins, and speci®c DNA binding sites (VDREs). Ligand-mediated conformational changes of the VDR are the core of the molecular switch of nuclear 1a,25(OH)2D3 signalling. Studying the interaction of 1a,25( ...
... vitamin D receptor (VDR) via combined contact with the retinoid X receptor (RXR), coactivator proteins, and speci®c DNA binding sites (VDREs). Ligand-mediated conformational changes of the VDR are the core of the molecular switch of nuclear 1a,25(OH)2D3 signalling. Studying the interaction of 1a,25( ...
the scf ubiquitin ligase: insights into a molecular machine
... (LRR)), the domain is an arc-shaped α–β-repeat structure that is also found in many protein-binding contexts, including the extracellular-binding domain of certain surface receptors11,12. In most cases, FBLs also seem to involve substrate phosphorylation for their interaction, but this does not seem ...
... (LRR)), the domain is an arc-shaped α–β-repeat structure that is also found in many protein-binding contexts, including the extracellular-binding domain of certain surface receptors11,12. In most cases, FBLs also seem to involve substrate phosphorylation for their interaction, but this does not seem ...
genetic analysis of hormone signaling
... cells, tissues, or even species of plants use all or only some of the same steps? The application of genetic analysis to hormone signaling has begun to provide some answers. Genetics is a powerful tool for establishing in vivo links between the signal and the response because inferences can be made ...
... cells, tissues, or even species of plants use all or only some of the same steps? The application of genetic analysis to hormone signaling has begun to provide some answers. Genetics is a powerful tool for establishing in vivo links between the signal and the response because inferences can be made ...
BCH401G Lecture 39 Andres Lecture Summary: Ribosome
... Another name for a charged tRNA is an aminoacyl tRNA. This is why it's binding site on the ribosome is called the aminoacyl site. The ribosome must then examine the different charged tRNAs that diffuse into the "A" site until it finds one with an anticodon that base-pairs correctly with the codon fo ...
... Another name for a charged tRNA is an aminoacyl tRNA. This is why it's binding site on the ribosome is called the aminoacyl site. The ribosome must then examine the different charged tRNAs that diffuse into the "A" site until it finds one with an anticodon that base-pairs correctly with the codon fo ...
C1. The start codon begins at the fifth nucleotide. The amino acid
... Proteins destined for the ER, Golgi, lysosomes, plasma membrane, or secretion have an SRP sorting signal at their amino terminal end. Nuclear proteins have an NLS sequence, etc. These sorting signals are recognized by cellular proteins/complexes that then act in a way to traffic the proteins to thei ...
... Proteins destined for the ER, Golgi, lysosomes, plasma membrane, or secretion have an SRP sorting signal at their amino terminal end. Nuclear proteins have an NLS sequence, etc. These sorting signals are recognized by cellular proteins/complexes that then act in a way to traffic the proteins to thei ...
Document
... Proteins destined for the ER, Golgi, lysosomes, plasma membrane, or secretion have an SRP sorting signal at their amino terminal end. Nuclear proteins have an NLS sequence, etc. These sorting signals are recognized by cellular proteins/complexes that then act in a way to traffic the proteins to thei ...
... Proteins destined for the ER, Golgi, lysosomes, plasma membrane, or secretion have an SRP sorting signal at their amino terminal end. Nuclear proteins have an NLS sequence, etc. These sorting signals are recognized by cellular proteins/complexes that then act in a way to traffic the proteins to thei ...
Extended Detail on Checklist items
... extraordinary result to a mapping to a commonly-observed protein. Consider if a known single aminoacid variation (SAAV) in neXtProt could turn an extraordinary result into an ordinary result. Consider if a single amino-acid change, not yet annotated in a well-known source, could turn an extraordinar ...
... extraordinary result to a mapping to a commonly-observed protein. Consider if a known single aminoacid variation (SAAV) in neXtProt could turn an extraordinary result into an ordinary result. Consider if a single amino-acid change, not yet annotated in a well-known source, could turn an extraordinar ...
Classifier ensembles for protein structural class prediction with
... prediction by using alternative sequence representations. Examples include auto-correlation functions based on non-bonded residue energy [7], polypeptide composition [26,37,44], and functional domain composition [16]. However, these algorithms are often only tested on very small datasets, with uncon ...
... prediction by using alternative sequence representations. Examples include auto-correlation functions based on non-bonded residue energy [7], polypeptide composition [26,37,44], and functional domain composition [16]. However, these algorithms are often only tested on very small datasets, with uncon ...
Lecture 2
... adjacent strand through hydrogen bonds. Amino acids in anti-parallel strands on the other hand interact with only one amino acid on an adjacent strand. Protein secondary structures – Turns & Loops: Almost all proteins exhibit a compact, globular structure which is possible only if there are turns or ...
... adjacent strand through hydrogen bonds. Amino acids in anti-parallel strands on the other hand interact with only one amino acid on an adjacent strand. Protein secondary structures – Turns & Loops: Almost all proteins exhibit a compact, globular structure which is possible only if there are turns or ...
Abscisic Acid–Induced Transcription Is Mediated by
... 2000; Uno et al., 2000). These factors were identified on the basis of their ability to bind ABREs by the yeast one-hybrid screen. Some of these factors have been shown to activate an ABA-responsive promoter and to enhance its ABA responsiveness (Uno et al., 2000). Because ABA-induced transcription ...
... 2000; Uno et al., 2000). These factors were identified on the basis of their ability to bind ABREs by the yeast one-hybrid screen. Some of these factors have been shown to activate an ABA-responsive promoter and to enhance its ABA responsiveness (Uno et al., 2000). Because ABA-induced transcription ...
Protein transport across the endoplasmic reticulum membrane
... A decisive step in the biosynthesis of many eukaryotic proteins is their partial or complete translocation across the endoplasmic reticulum membrane. A similar process occurs in prokaryotes, except that proteins are transported across or are integrated into the plasma membrane. In both cases, transl ...
... A decisive step in the biosynthesis of many eukaryotic proteins is their partial or complete translocation across the endoplasmic reticulum membrane. A similar process occurs in prokaryotes, except that proteins are transported across or are integrated into the plasma membrane. In both cases, transl ...
Role of hsp90 and the hsp90-binding immunophilins in signalling
... The ubiquitous protein chaperone hsp90 has been shown to regulate more than 100 proteins involved in cellular signalling. These proteins are called ‘client proteins’ for hsp90, and a multiprotein hsp90/hsp70-based chaperone machinery forms client protein hsp90 heterocomplexes in the cytoplasm and th ...
... The ubiquitous protein chaperone hsp90 has been shown to regulate more than 100 proteins involved in cellular signalling. These proteins are called ‘client proteins’ for hsp90, and a multiprotein hsp90/hsp70-based chaperone machinery forms client protein hsp90 heterocomplexes in the cytoplasm and th ...
Chapter 23: Meat Emulsions - FEA
... close to each other (McClements 2005). In a number of cases, emulsifiers are necessary to facilitate emulsion formation (Schramm 2005). Many emulsifiers are amphiphilic molecules, with polar and nonpolar regions within the same molecule (McClements 2005). The ability of fat emulsifier to form an em ...
... close to each other (McClements 2005). In a number of cases, emulsifiers are necessary to facilitate emulsion formation (Schramm 2005). Many emulsifiers are amphiphilic molecules, with polar and nonpolar regions within the same molecule (McClements 2005). The ability of fat emulsifier to form an em ...
Functional studies of the BTB domain in the Drosophila GAGA and
... derived from cDNA clones, and following purification of positive clones, the isolates representing ttk, trl and broad (br) cDNAs were identified. The remaining clones were then assigned to groups based on restriction mapping and Southern analysis, and in the case of seven of them, by cytogenetic map ...
... derived from cDNA clones, and following purification of positive clones, the isolates representing ttk, trl and broad (br) cDNAs were identified. The remaining clones were then assigned to groups based on restriction mapping and Southern analysis, and in the case of seven of them, by cytogenetic map ...
Protein Quality Control as a Strategy for Cellular Regulation
... Randolph Hampton was born in New York State just before cars evolved fins. He grew up in Scarsdale, NY, attended Ohio Wesleyan University, and by a circuitous route that included research at University of Michigan, running the office at Zingerman’s Delicatessen, and doing field work in music and com ...
... Randolph Hampton was born in New York State just before cars evolved fins. He grew up in Scarsdale, NY, attended Ohio Wesleyan University, and by a circuitous route that included research at University of Michigan, running the office at Zingerman’s Delicatessen, and doing field work in music and com ...
Unexpected Diversity of Signal Peptides in Prokaryotes
... the proteome as a whole. The proteomechaffeensis (n ⫽ 18); (D) Pelagibacter ubique (n ⫽ 28); (E) Methanospirillum hungatei (n ⫽ 42); (F) wide amino acid composition of the nine AlCyanothece strain ATCC 51142 (n ⫽ 31); (G) Chlorobaculum tepidum (n ⫽ 19); (H) Mycobacterium phaproteobacteria shows a cl ...
... the proteome as a whole. The proteomechaffeensis (n ⫽ 18); (D) Pelagibacter ubique (n ⫽ 28); (E) Methanospirillum hungatei (n ⫽ 42); (F) wide amino acid composition of the nine AlCyanothece strain ATCC 51142 (n ⫽ 31); (G) Chlorobaculum tepidum (n ⫽ 19); (H) Mycobacterium phaproteobacteria shows a cl ...
IdaPro® Milk Proteins - SupplySide Storefronts
... amino acids available and therefore will experience slower recovery and a gradual loss in size and strength of skeletal muscle tissue. A lesser known benefit of milk protein is the highly bioavailable milk mineral content, predominantly calcium and phosphorous, which help to build stronger skeletal ...
... amino acids available and therefore will experience slower recovery and a gradual loss in size and strength of skeletal muscle tissue. A lesser known benefit of milk protein is the highly bioavailable milk mineral content, predominantly calcium and phosphorous, which help to build stronger skeletal ...
DOCX - The Human Proteome Project
... to a mapping to a commonly-observed protein. Consider if a known single amino-acid variation (SAAV) in neXtProt could turn an extraordinary result into an ordinary result. Consider if a single amino-acid change, not yet annotated in a well-known source, could turn an extraordinary result into a ques ...
... to a mapping to a commonly-observed protein. Consider if a known single amino-acid variation (SAAV) in neXtProt could turn an extraordinary result into an ordinary result. Consider if a single amino-acid change, not yet annotated in a well-known source, could turn an extraordinary result into a ques ...
TAP(Tandem Affinity Purification)
... and can create protein network maps • Analyzes more of the proteome • Works in membrane proteins • Works in many organisms ...
... and can create protein network maps • Analyzes more of the proteome • Works in membrane proteins • Works in many organisms ...
Computational Tools For Protein Modeling
... expectation value for an alignment, which estimates how many times one expects to see such an alignment occur by chance. This allows a user to quantitatively assess the significance of the alignment. Although it may not be as sensitive as many other tools, BLAST captures most of the possible matches ...
... expectation value for an alignment, which estimates how many times one expects to see such an alignment occur by chance. This allows a user to quantitatively assess the significance of the alignment. Although it may not be as sensitive as many other tools, BLAST captures most of the possible matches ...
Adaptative biochemical pathways and regulatory networks in
... reveal proteins whose abundance is iron-dependent during anaerobic growth. In all cases, BAS10 biomass samples were all harvested at late exponential growth phases, when anaerobic growth on FEC is coupled with iron-binding EPS production [12, 16] at an average yield of 6.7 g/L (dry weight EPS per cu ...
... reveal proteins whose abundance is iron-dependent during anaerobic growth. In all cases, BAS10 biomass samples were all harvested at late exponential growth phases, when anaerobic growth on FEC is coupled with iron-binding EPS production [12, 16] at an average yield of 6.7 g/L (dry weight EPS per cu ...
Pavel Doležal
... characterized as a double membrane-bound organelle that produces ATP by oxidative phosphorylation. The process is dependent on consumption of oxygen as the terminal electron acceptor, which is coupled with the citric acid cycle generating reducing equivalents. Additionally, the mitochondria are invo ...
... characterized as a double membrane-bound organelle that produces ATP by oxidative phosphorylation. The process is dependent on consumption of oxygen as the terminal electron acceptor, which is coupled with the citric acid cycle generating reducing equivalents. Additionally, the mitochondria are invo ...
Pavel Doležal
... characterized as a double membrane-bound organelle that produces ATP by oxidative phosphorylation. The process is dependent on consumption of oxygen as the terminal electron acceptor, which is coupled with the citric acid cycle generating reducing equivalents. Additionally, the mitochondria are invo ...
... characterized as a double membrane-bound organelle that produces ATP by oxidative phosphorylation. The process is dependent on consumption of oxygen as the terminal electron acceptor, which is coupled with the citric acid cycle generating reducing equivalents. Additionally, the mitochondria are invo ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).