Srivastava, Sanjay: Analysis of Methods for Predicting Protein Fold and Remote Homologue Recognition
... (1D) involves the kind of fold that the given amino acid sequence may adopt. If similarity between two proteins is detectable at the sequence level, structural similarity can usually be assumed, because the 3D structures of proteins from the same family are more conserved than their primary sequence ...
... (1D) involves the kind of fold that the given amino acid sequence may adopt. If similarity between two proteins is detectable at the sequence level, structural similarity can usually be assumed, because the 3D structures of proteins from the same family are more conserved than their primary sequence ...
Mass spectrometry and proteomics Steven P Gygi* and Ruedi
... that is covalently derivatized with trypsin. The generated peptides are then trapped on a membrane and identified by MALDI−TOF mass fingerprinting. The overall 2DE−MS approach has been used to generate annotated 2D gel databases for a myriad of cell types from different species. A partial list of su ...
... that is covalently derivatized with trypsin. The generated peptides are then trapped on a membrane and identified by MALDI−TOF mass fingerprinting. The overall 2DE−MS approach has been used to generate annotated 2D gel databases for a myriad of cell types from different species. A partial list of su ...
HiPer® Affinity Chromatography Teaching Kit
... Affinity chromatography is a very effective molecular technique for purification of protein on the basis of its biological function. Through this chromatography the desired protein is isolated from a mixed solution depending upon the protein's specific binding affinity to ligands mounted in a gel ma ...
... Affinity chromatography is a very effective molecular technique for purification of protein on the basis of its biological function. Through this chromatography the desired protein is isolated from a mixed solution depending upon the protein's specific binding affinity to ligands mounted in a gel ma ...
protein
... In receptor-mediated endocytosis, binding of ligands to receptors triggers vesicle formation A ligand is any molecule that binds specifically to a receptor site of another molecule Receptor mediated endocytosis lets the cell be selective about what molecules come into the cell in vesicles. ...
... In receptor-mediated endocytosis, binding of ligands to receptors triggers vesicle formation A ligand is any molecule that binds specifically to a receptor site of another molecule Receptor mediated endocytosis lets the cell be selective about what molecules come into the cell in vesicles. ...
Comparing Sequences of Fluorescent Proteins Using
... research (see image at right). This diversity of colors among fluorescent proteins has sometimes been referred to as the “mFruits,” referring to the names given to these fluorescent proteins, such as: • mBlueberry (Blue Fluorescent Protein, or BFP) • mLemon (Yellow Fluorescent Protein, or YFP) • mGr ...
... research (see image at right). This diversity of colors among fluorescent proteins has sometimes been referred to as the “mFruits,” referring to the names given to these fluorescent proteins, such as: • mBlueberry (Blue Fluorescent Protein, or BFP) • mLemon (Yellow Fluorescent Protein, or YFP) • mGr ...
(PTH), or parathormone, is secreted
... blood by acting upon parathyroid hormone receptorin three parts of the body: In the bones- It enhances the release of calcium from the large reservoir contained in the bones. Bone resorption is the normal destruction of bone by osteoclasts, which are indirectly stimulated by PTH. Stimulation is indi ...
... blood by acting upon parathyroid hormone receptorin three parts of the body: In the bones- It enhances the release of calcium from the large reservoir contained in the bones. Bone resorption is the normal destruction of bone by osteoclasts, which are indirectly stimulated by PTH. Stimulation is indi ...
elucidate the contribution of proteins to tears. a challenge for
... Beneath the dominant, and almost dismissive idea that the roles for proteins in the tears are antimicrobial and immunological, has been a persistent undercurrent from stalwarts investigating the physical contribution of proteins to tears. It is here that we enter the realms of surface tension and vi ...
... Beneath the dominant, and almost dismissive idea that the roles for proteins in the tears are antimicrobial and immunological, has been a persistent undercurrent from stalwarts investigating the physical contribution of proteins to tears. It is here that we enter the realms of surface tension and vi ...
Eukaryotic-type protein kinases in Streptomyces
... also other bacterial genomes. Based on this, we suggest possible roles for these enzymes in the regulation of cellular processes in streptomycetes. The S. coelicolor A3(2) genome carries 34 putative ESTPK genes Based on the screening of the chromosome sequence data (Bentley et al., 2002) using the s ...
... also other bacterial genomes. Based on this, we suggest possible roles for these enzymes in the regulation of cellular processes in streptomycetes. The S. coelicolor A3(2) genome carries 34 putative ESTPK genes Based on the screening of the chromosome sequence data (Bentley et al., 2002) using the s ...
Heavy Metal Stress. Activation of Distinct Mitogen
... Cadmium- and copper-responsive genes have been shown to code for signal transduction components, such as the Arabidopsis mitogen-activated protein kinase kinase kinase (MAPKKK) MEKK1, transcription factors, stress-induced proteins, proteins participating in protein folding, and sulfur and glutathion ...
... Cadmium- and copper-responsive genes have been shown to code for signal transduction components, such as the Arabidopsis mitogen-activated protein kinase kinase kinase (MAPKKK) MEKK1, transcription factors, stress-induced proteins, proteins participating in protein folding, and sulfur and glutathion ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... 3.1 Homology modeling Homology modeling is a very effective method of generating the 3D structure of a protein for which experimental (X-rays crystallographic or NMR) structure is not available but the crystal structure of homologue is available. Then by making use of this template the 3D structure ...
... 3.1 Homology modeling Homology modeling is a very effective method of generating the 3D structure of a protein for which experimental (X-rays crystallographic or NMR) structure is not available but the crystal structure of homologue is available. Then by making use of this template the 3D structure ...
HIV-1 and Ebola virus encode small peptide motifs that recruit
... - PTAp sequence is required for Ebola virus particles formation. In both HIV and ebola virus PTAP is the motif which interacts with Tsg101 3 Is Tsg 101 relocalize to the site of budding? Yes, in the presence of active or wt Ebola Vp40 Tsg localizes ...
... - PTAp sequence is required for Ebola virus particles formation. In both HIV and ebola virus PTAP is the motif which interacts with Tsg101 3 Is Tsg 101 relocalize to the site of budding? Yes, in the presence of active or wt Ebola Vp40 Tsg localizes ...
Manuscript - CSIRO Research Publications Repository
... the plant PTI and ETI systems highlighting recent advances as well as identifying key gaps in our understanding of these processes. Finally we discuss briefly how this knowledge is beginning to feed back into the agricultural context that originally spawned the study of plant immunity. ...
... the plant PTI and ETI systems highlighting recent advances as well as identifying key gaps in our understanding of these processes. Finally we discuss briefly how this knowledge is beginning to feed back into the agricultural context that originally spawned the study of plant immunity. ...
Materials and Methods - UROP
... of substrate). The biosensor device contrasts the old method of viewing enzymes, which only examines average effects, typically looking at the starting and ending points. Significantly, the biosensor device can not only be used to study the behavior of unknown proteins, but could also potentially b ...
... of substrate). The biosensor device contrasts the old method of viewing enzymes, which only examines average effects, typically looking at the starting and ending points. Significantly, the biosensor device can not only be used to study the behavior of unknown proteins, but could also potentially b ...
Improved insights into protein thermal stability: from the
... where k is the Boltzmann constant. These probabilities can be estimated in terms of the number of occurrences n(c, s), n(c) n(s) and n in a structure dataset (see [5] for details). The potentials so defined conserve the memory of the protein dataset from which they are extracted. It is generally adm ...
... where k is the Boltzmann constant. These probabilities can be estimated in terms of the number of occurrences n(c, s), n(c) n(s) and n in a structure dataset (see [5] for details). The potentials so defined conserve the memory of the protein dataset from which they are extracted. It is generally adm ...
Divergent or just different Rozeboom, Henriette
... limitations to the development of new functionalities. It is believed that two wholegenome duplication events (during meiosis) in the globin gene superfamily in the stem lineage of vertebrates were necessary for promoting evolutionary innovation (Storz et al., 2013). Hemoglobin and myoglobin are goo ...
... limitations to the development of new functionalities. It is believed that two wholegenome duplication events (during meiosis) in the globin gene superfamily in the stem lineage of vertebrates were necessary for promoting evolutionary innovation (Storz et al., 2013). Hemoglobin and myoglobin are goo ...
New high-throughput NMR
... assessed in orthotopic mouse models. Giese reveals that, so far, they have found four new targets that have been validated ...
... assessed in orthotopic mouse models. Giese reveals that, so far, they have found four new targets that have been validated ...
Food Proteins and Enzymes
... From point of view of food safety it should be mentioned the several plants contain antinutritive factors, which may be present as contaminants in protein concentrates and isolates. Natural enzyme inhibitors, enzymes, phytic acid, and lectines are the most important. For a complex view it should fin ...
... From point of view of food safety it should be mentioned the several plants contain antinutritive factors, which may be present as contaminants in protein concentrates and isolates. Natural enzyme inhibitors, enzymes, phytic acid, and lectines are the most important. For a complex view it should fin ...
TONE UP. GET LEAN. BE STRONG.
... The body is able to make non-essential amino acids from other amino acids in the body. The body, however, is not able to make essential amino acids; the only way to get them is through diet. One of the best ways to ensure you are meeting your needs is by eating highquality protein foods. Protein sou ...
... The body is able to make non-essential amino acids from other amino acids in the body. The body, however, is not able to make essential amino acids; the only way to get them is through diet. One of the best ways to ensure you are meeting your needs is by eating highquality protein foods. Protein sou ...
Polymer - Deans Community High School
... All proteins contain the elements C,N,O,H. They are condensation polymers, made by amino acids linking together. An amine group of one molecule links to the carboxyl group of another molecule to form an amide or peptide bond. The body makes specific proteins it needs, but cannot make all the amino a ...
... All proteins contain the elements C,N,O,H. They are condensation polymers, made by amino acids linking together. An amine group of one molecule links to the carboxyl group of another molecule to form an amide or peptide bond. The body makes specific proteins it needs, but cannot make all the amino a ...
Regulatory Strategies
... Enzymes exist in active and inactive forms – Interconvertable by covalent modification • Catalyzed by other enzymes • Most modifications are reversible ...
... Enzymes exist in active and inactive forms – Interconvertable by covalent modification • Catalyzed by other enzymes • Most modifications are reversible ...
Protein Family Analysis: Protein Family Sorter
... 1. To get the data and names on protein families, use the mouse to draw a box around the area of interest in the heatmap (Red arrow 1 in Panel A). A pop-‐up window will appear that al ...
... 1. To get the data and names on protein families, use the mouse to draw a box around the area of interest in the heatmap (Red arrow 1 in Panel A). A pop-‐up window will appear that al ...
Author`s personal copy - Ruhr
... firing might be mediated through a decrease in K+ conductance, for example, by KCNQ channels, which are inhibited by activation of Gq-coupled receptors. If this is the case, then chARGe would have to be expressed in neurons with sufficient M-currents to be able to affect neuronal firing. Second, the ...
... firing might be mediated through a decrease in K+ conductance, for example, by KCNQ channels, which are inhibited by activation of Gq-coupled receptors. If this is the case, then chARGe would have to be expressed in neurons with sufficient M-currents to be able to affect neuronal firing. Second, the ...
Intrinsically Disordered Domains of the B Cell Receptor
... protein synthesis (CFPS) offers several advantages compared to conventional in vivo synthesis for the production of IDPs. In this thesis, an integrated approach for efficient characterization of IDPs has been developed, combining CFPS and novel NMR methodology with fast spectroscopy and self-validat ...
... protein synthesis (CFPS) offers several advantages compared to conventional in vivo synthesis for the production of IDPs. In this thesis, an integrated approach for efficient characterization of IDPs has been developed, combining CFPS and novel NMR methodology with fast spectroscopy and self-validat ...
Morphologically distinct phenotypes of spermatozoa in infertile men
... RESULTS: A total of 1202 proteins were identified in the F1 fraction while 1140, 1025 and 890 proteins were recovered from the three other fractions, F2, F3 and F4 respectively. With respect to the differentially expressed proteins, F1 exhibited the highest number (522), followed by F2 (362) and low ...
... RESULTS: A total of 1202 proteins were identified in the F1 fraction while 1140, 1025 and 890 proteins were recovered from the three other fractions, F2, F3 and F4 respectively. With respect to the differentially expressed proteins, F1 exhibited the highest number (522), followed by F2 (362) and low ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).