Computational design of a pH-sensitive IgG binding
... balance between affinity and the pH dependence of binding, we constructed a library guided by the deep-sequencing data and Rosetta energy calculations and carried out rounds of selection for increased binding affinity at pH 6.5 and 8 (Fig. S5). In the library, a single core substitution L166F was in ...
... balance between affinity and the pH dependence of binding, we constructed a library guided by the deep-sequencing data and Rosetta energy calculations and carried out rounds of selection for increased binding affinity at pH 6.5 and 8 (Fig. S5). In the library, a single core substitution L166F was in ...
Protein and Minerals in the Athlete`s Diet
... exercised. When the cycle is completed time and time again, stronger muscles result. However, muscle tissue repair cannot proceed unless sufficient amino acids are present in the blood and tissues to feed the muscle-building mechanism. Proper protein nutrition is essential for a hard training athlet ...
... exercised. When the cycle is completed time and time again, stronger muscles result. However, muscle tissue repair cannot proceed unless sufficient amino acids are present in the blood and tissues to feed the muscle-building mechanism. Proper protein nutrition is essential for a hard training athlet ...
09_chapter 4
... purification but may be used for purification of oligonucleotides, peptides, or other charged molecules. The protein of interest must have a charge opposite that of the functional group attached to the resin in order to bind. For example, immunoglobulins, which generally have an overall positive cha ...
... purification but may be used for purification of oligonucleotides, peptides, or other charged molecules. The protein of interest must have a charge opposite that of the functional group attached to the resin in order to bind. For example, immunoglobulins, which generally have an overall positive cha ...
Structural and functional features of the intracellular amino
... preceding the conserved aminoterminal region in αENaC. In this context, it is intriguing that two nematode proteins, MEC-2 and UNC-1, that have been proposed to interact with and modulate degenerin channels, contain a domain implicated in regulating proteolysis of membrane-associated proteins [18]. ...
... preceding the conserved aminoterminal region in αENaC. In this context, it is intriguing that two nematode proteins, MEC-2 and UNC-1, that have been proposed to interact with and modulate degenerin channels, contain a domain implicated in regulating proteolysis of membrane-associated proteins [18]. ...
LESSON 11. СOMMUNICATION BETWEEN CELLS. MECHANISM
... Types. The effect of the active form (Gα-GTP) depends on the specific type of G protein. There are several different types of G proteins: Gs stimulates the enzyme adenylate cyclase, Gi inhibits the enzyme adenylate cyclase, and Gq stimulates the enzyme phospholipase C. Function. The Gα subunit of al ...
... Types. The effect of the active form (Gα-GTP) depends on the specific type of G protein. There are several different types of G proteins: Gs stimulates the enzyme adenylate cyclase, Gi inhibits the enzyme adenylate cyclase, and Gq stimulates the enzyme phospholipase C. Function. The Gα subunit of al ...
Viral mechanisms of immune evasion
... therefore multifunctional. This particular constraint is less rigid for the generation and selection of variants with different antigenic DNA viruses as their genome size allows a larger number of genes to properties that can evade recognition by neutralizing antibodies is be devoted to host control ...
... therefore multifunctional. This particular constraint is less rigid for the generation and selection of variants with different antigenic DNA viruses as their genome size allows a larger number of genes to properties that can evade recognition by neutralizing antibodies is be devoted to host control ...
Viral mechanisms of immune evasion
... therefore multifunctional. This particular constraint is less rigid for the generation and selection of variants with different antigenic DNA viruses as their genome size allows a larger number of genes to properties that can evade recognition by neutralizing antibodies is be devoted to host control ...
... therefore multifunctional. This particular constraint is less rigid for the generation and selection of variants with different antigenic DNA viruses as their genome size allows a larger number of genes to properties that can evade recognition by neutralizing antibodies is be devoted to host control ...
Expression and Purification of Toxoplasma gondii Cell Cycle
... cyclin proteins, the binding partner that activates CDKs, this is where Apicomplexans diverge from other Eukaryotes. The TgCYC1 and TgCYC2 genes are not human cyclinB, nor ...
... cyclin proteins, the binding partner that activates CDKs, this is where Apicomplexans diverge from other Eukaryotes. The TgCYC1 and TgCYC2 genes are not human cyclinB, nor ...
Polyacrylamide Gel Electrophoresis Analysis of Ribosomal Protein
... niveoalba) (Fig. 1B through D). These results, together with the results of a DNA-DNA pairing test (18), suggest that these three species might be combined into a single species. The extensive similarities were especially impressive for Microtetraspora roseoviolacea, Microtetraspora salmonea, and Mi ...
... niveoalba) (Fig. 1B through D). These results, together with the results of a DNA-DNA pairing test (18), suggest that these three species might be combined into a single species. The extensive similarities were especially impressive for Microtetraspora roseoviolacea, Microtetraspora salmonea, and Mi ...
The a-subunit of a heterotrimeric G-protein from
... complex. Both the GTP-loaded a-subunit and the bccomplex can transduce the signal to a variety of effectors such as adenylylcyclase, phospholipases and ion channels. The activated state of the G-protein is terminated when the bound GTP is hydrolysed by the GTPase activity of the a-subunit. In plants ...
... complex. Both the GTP-loaded a-subunit and the bccomplex can transduce the signal to a variety of effectors such as adenylylcyclase, phospholipases and ion channels. The activated state of the G-protein is terminated when the bound GTP is hydrolysed by the GTPase activity of the a-subunit. In plants ...
Characterisation of new intracellular membranes in Escherichia coli
... proliferation in E. coli Subunit b was over-produced in C41(DE3) and C43(DE3) bacterial hosts. Maximal level of production of subunit b (30 mg/l of culture) was achieved in C43(DE3) cells 3 h after induction at 37³C or 18 h after induction at 25³C. Over-expression of subunit b was accompanied by pro ...
... proliferation in E. coli Subunit b was over-produced in C41(DE3) and C43(DE3) bacterial hosts. Maximal level of production of subunit b (30 mg/l of culture) was achieved in C43(DE3) cells 3 h after induction at 37³C or 18 h after induction at 25³C. Over-expression of subunit b was accompanied by pro ...
Re-routing the huntingtin protein inside cells
... seem to be particularly important. They behave a bit like an ‘address label’ for the whole protein. Depending on what’s happening to a cell, small chemical ‘tags’ can get attached to the first seventeen amino acids, or removed. The cell’s transport machinery can then read these tags like a bar code. ...
... seem to be particularly important. They behave a bit like an ‘address label’ for the whole protein. Depending on what’s happening to a cell, small chemical ‘tags’ can get attached to the first seventeen amino acids, or removed. The cell’s transport machinery can then read these tags like a bar code. ...
Proteomics Analysis of Immunoprecipitated Proteins Associated with
... from 133 to 388 is the kinase domain which has signifycant sequence similarity with kinase domains from other MAP3Ks. The putative C-terminal domain consists of the residues 389 to 467. It has been shown that C-terminally truncated Tpl-2 (oncogenic form) is approximately five times more active in au ...
... from 133 to 388 is the kinase domain which has signifycant sequence similarity with kinase domains from other MAP3Ks. The putative C-terminal domain consists of the residues 389 to 467. It has been shown that C-terminally truncated Tpl-2 (oncogenic form) is approximately five times more active in au ...
What Are The Best Supplements For Muscle Building
... Although Creatine can be found in food like steak and chicken, the only way to get all the benefit of Creatine is by taking supplements. Creatine has shown that creatine can pull water into muscle cell, increasing their volume and size. This results in your muscle looking more round and fuller. Crea ...
... Although Creatine can be found in food like steak and chicken, the only way to get all the benefit of Creatine is by taking supplements. Creatine has shown that creatine can pull water into muscle cell, increasing their volume and size. This results in your muscle looking more round and fuller. Crea ...
Structure-activity Relationships in Flexible Protein Domains
... inhibit GDP dissociation as well, but have not been evaluated as inhibitors of GTP hydrolysis. The physiological role of the interaction of RhoGDI with the GTP-bound form of a GTPase remains to be established. Overall, when exogenously introduced into cells the GDIs behave as negative regulators by ...
... inhibit GDP dissociation as well, but have not been evaluated as inhibitors of GTP hydrolysis. The physiological role of the interaction of RhoGDI with the GTP-bound form of a GTPase remains to be established. Overall, when exogenously introduced into cells the GDIs behave as negative regulators by ...
ATP - Mhanafi123`s Blog
... glucose is transported across the plasma membrane by membrane associated carrier proteins, glucose transporters. There are 2 different types of transporter proteins, which mediate the transfer of glucose and other sugars through the lipid bilayer: ...
... glucose is transported across the plasma membrane by membrane associated carrier proteins, glucose transporters. There are 2 different types of transporter proteins, which mediate the transfer of glucose and other sugars through the lipid bilayer: ...
Membrane Protein Sorting in the Yeast Secretory Pathway
... indicated above. DPAP B and DPAP A are predicted to contain 841 and 931 amino acids, respectively. The amino acid sequence of the cytoplasmic domain of DPAP A is indicated in the one letter amino acid code, and the boxed residues are removed by the A22-AAA mutation (see below). (B) Constructs used i ...
... indicated above. DPAP B and DPAP A are predicted to contain 841 and 931 amino acids, respectively. The amino acid sequence of the cytoplasmic domain of DPAP A is indicated in the one letter amino acid code, and the boxed residues are removed by the A22-AAA mutation (see below). (B) Constructs used i ...
Protein Targeting into the Complex Plastid of Cryptophytes
... consequence, are excellent indicators for the evolution of these topogenic signals in general. At least parts of another biochemical pathway, carotenoid synthesis, were also predicted to be localized inside the PPC (Douglas et al. 2001), as geranylgeranyltransferase was found to be encoded on the nu ...
... consequence, are excellent indicators for the evolution of these topogenic signals in general. At least parts of another biochemical pathway, carotenoid synthesis, were also predicted to be localized inside the PPC (Douglas et al. 2001), as geranylgeranyltransferase was found to be encoded on the nu ...
Protein Prenylation: Genes, Enzymes, Targets, and Functions
... Annu. Rev. Genet. 1992.26:209-237. Downloaded from arjournals.annualreviews.org by Medical Research Council-Cambridge UK on 11/21/06. For personal use only. ...
... Annu. Rev. Genet. 1992.26:209-237. Downloaded from arjournals.annualreviews.org by Medical Research Council-Cambridge UK on 11/21/06. For personal use only. ...
to get the file - Chair of Computational Biology
... messenger that is important in many biological processes. cAMP is derived from ATP and used for intracellular signal transduction in many different organisms, conveying the cAMP dependent pathway. In humans, cyclic AMP works by activating cAMPdependent protein kinase. Cyclic AMP binds to specific lo ...
... messenger that is important in many biological processes. cAMP is derived from ATP and used for intracellular signal transduction in many different organisms, conveying the cAMP dependent pathway. In humans, cyclic AMP works by activating cAMPdependent protein kinase. Cyclic AMP binds to specific lo ...
Protein Crystallization
... ¾ loss of entropy due to ordering of otherwise flexible loops, chain termini or domain flexibility; the magnitude will vary and is impossible to estimate ¾ the structure of the crystal contacts and in particular the extend of entropy loss by side chains trapped between the molecules. ...
... ¾ loss of entropy due to ordering of otherwise flexible loops, chain termini or domain flexibility; the magnitude will vary and is impossible to estimate ¾ the structure of the crystal contacts and in particular the extend of entropy loss by side chains trapped between the molecules. ...
Introduced amino terminal epitopes can reduce surface expression
... α4 or β4 subunits, the V5 epitope has little effect when placed in either, while the Myc epitope reduces expression more when inserted into β4 than α4. These results indicate that the extreme amino terminal region is important for assembly of these receptors, and demonstrate that some widely used in ...
... α4 or β4 subunits, the V5 epitope has little effect when placed in either, while the Myc epitope reduces expression more when inserted into β4 than α4. These results indicate that the extreme amino terminal region is important for assembly of these receptors, and demonstrate that some widely used in ...
Gene Section IGFBP6 (insulin-like growth factor binding protein 6)
... actions (Bach, 1999; Bach, 2005; Bach et al., 2013). It is antiproliferative and proapoptotic in a number of cell lines in vitro (Bach, 1999; Bach, 2005; Bach et al., 2013). At least some of its actions in regulating cell fate are mediated by interaction with Ku80, a DNA-end binding protein (Iosef e ...
... actions (Bach, 1999; Bach, 2005; Bach et al., 2013). It is antiproliferative and proapoptotic in a number of cell lines in vitro (Bach, 1999; Bach, 2005; Bach et al., 2013). At least some of its actions in regulating cell fate are mediated by interaction with Ku80, a DNA-end binding protein (Iosef e ...
1X Equilibration/Wash Buffer (pH 7.0)
... 21. Add 2 volumes (200 ul) of the 1X Imidazole Elution Buffer to the resin, and resuspend by 22. Centrifuge at 1000 rpm for 5 min. collect the supernatant. 23. Repeat Steps 21 and 22. (Do not forget to collect the supernatant.) ...
... 21. Add 2 volumes (200 ul) of the 1X Imidazole Elution Buffer to the resin, and resuspend by 22. Centrifuge at 1000 rpm for 5 min. collect the supernatant. 23. Repeat Steps 21 and 22. (Do not forget to collect the supernatant.) ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).