Dietary plant-protein substitution affects hepatic metabolism in
... The high dietary protein requirements of salmonid fish are met with fishmeal-based feed in commercial aquaculture. The sustainability of this practice is questionable and, therefore, the feasibility of substituting fishmeal with plant-based products needs to be investigated. We investigated growth a ...
... The high dietary protein requirements of salmonid fish are met with fishmeal-based feed in commercial aquaculture. The sustainability of this practice is questionable and, therefore, the feasibility of substituting fishmeal with plant-based products needs to be investigated. We investigated growth a ...
Biochemistry 2000 Sample Questions Proteins
... SDS-PAGE in the presence of reducing agent (2-mercaptoethanol) will denature the protein and break any disulfide bonds. There are two possible results: case 1: two or more bands are present and indicate the protein has multiple subunits case 2: one band is present. This is an inconclusive result as ...
... SDS-PAGE in the presence of reducing agent (2-mercaptoethanol) will denature the protein and break any disulfide bonds. There are two possible results: case 1: two or more bands are present and indicate the protein has multiple subunits case 2: one band is present. This is an inconclusive result as ...
Resurrecting ancestral RuBisCO in silico
... The evolution of ancient organisms both shaped and was shaped by drastic global environmental changes such as the Great Oxygenation Event (GOE). This rapid atmospheric transition is believed to be broadly coincident with the diversification of cellular life on earth.1-3 The enzyme ribulose 1,5-bisph ...
... The evolution of ancient organisms both shaped and was shaped by drastic global environmental changes such as the Great Oxygenation Event (GOE). This rapid atmospheric transition is believed to be broadly coincident with the diversification of cellular life on earth.1-3 The enzyme ribulose 1,5-bisph ...
I + rel + - UCSF Biochemistry & Biophysics
... • ppGpp slows down translation at multiple steps of translation • The aa-tRNA for the hungry codon is only reduced enough to generate a signal A C D E F G H I K L M N P Q R S T V W Y ...
... • ppGpp slows down translation at multiple steps of translation • The aa-tRNA for the hungry codon is only reduced enough to generate a signal A C D E F G H I K L M N P Q R S T V W Y ...
supplementary text
... Network analysis: Various network properties viz. hubs, clusters and k-cliques were analyzed. ‘K-cliques’ is one such network component wherein if a network is created by “k” nodes, each node in such a clique is connected to each other by an edge. Edges represent the non-covalent interactions betwee ...
... Network analysis: Various network properties viz. hubs, clusters and k-cliques were analyzed. ‘K-cliques’ is one such network component wherein if a network is created by “k” nodes, each node in such a clique is connected to each other by an edge. Edges represent the non-covalent interactions betwee ...
Oxidative stress in bacteria and protein damage by reactive oxygen
... can restore the redox homeostasis of the cytosol and eliminate harmful oxidant by activation of the transcription factors SoxRS and OxyR. Irreversible oxidation of amino acid residues in a protein can be exerted by two major mechanisms: ionizing radiation and metal ion-catalyzed oxidation reactions. ...
... can restore the redox homeostasis of the cytosol and eliminate harmful oxidant by activation of the transcription factors SoxRS and OxyR. Irreversible oxidation of amino acid residues in a protein can be exerted by two major mechanisms: ionizing radiation and metal ion-catalyzed oxidation reactions. ...
Protein Secretion in Plants: from the trans
... Vesicle budding requires small GTPases of the ARF family, their guanine-nucleotide exchange factors (ARF-GEFs) and GTPase-activating proteins (ARF-GAPs) for coat recruitment and cargo selection. ARFs not only act to recruit COPI and clathrin coats to membranes but also play a role in the control of ...
... Vesicle budding requires small GTPases of the ARF family, their guanine-nucleotide exchange factors (ARF-GEFs) and GTPase-activating proteins (ARF-GAPs) for coat recruitment and cargo selection. ARFs not only act to recruit COPI and clathrin coats to membranes but also play a role in the control of ...
Detection of Cellular Response to an in vitro Challenge with
... was set to 100 ms. Dynamic exclusion is turned on using a peak width of 60 s. ...
... was set to 100 ms. Dynamic exclusion is turned on using a peak width of 60 s. ...
Synapse Specificity Minireview and Long
... the behaviors mediated by these synapses may not require a high degree of synapse specificity. More generally, just how specific do changes in synaptic efficacy need to be? Most arguments for synapse specificity are based on the belief that the complexity and abundance of the synaptic architecture m ...
... the behaviors mediated by these synapses may not require a high degree of synapse specificity. More generally, just how specific do changes in synaptic efficacy need to be? Most arguments for synapse specificity are based on the belief that the complexity and abundance of the synaptic architecture m ...
Sec14p-like proteins regulate phosphoinositide homoeostasis and
... PITPs (phosphatidylinositol-transfer proteins) are characterized by their ability to mediate the transfer of phosphatidylinositol or PC (phosphatidylcholine) monomers between membrane bilayers in vitro [1,2]. The major PITP in the yeast Saccharomyces cerevisiae, encoded by the essential SEC14 gene, ...
... PITPs (phosphatidylinositol-transfer proteins) are characterized by their ability to mediate the transfer of phosphatidylinositol or PC (phosphatidylcholine) monomers between membrane bilayers in vitro [1,2]. The major PITP in the yeast Saccharomyces cerevisiae, encoded by the essential SEC14 gene, ...
Lehninger Principles of Biochemistry
... Fibrous and Globular Proteins Many proteins can be classified into two groups based on their general structural features. Fibrous proteins, such as keratins and collagens, have polypeptide chains arranged in long strands or sheets. These proteins usually consist mostly of a single type of secondar ...
... Fibrous and Globular Proteins Many proteins can be classified into two groups based on their general structural features. Fibrous proteins, such as keratins and collagens, have polypeptide chains arranged in long strands or sheets. These proteins usually consist mostly of a single type of secondar ...
Lecture 11 Part 2
... cell to produce hormone’s effects. • Modulates activity of enzymes present in the cell. • Alters metabolism of the cell. • cAMP inactivated by phosphodiesterase. – Hydrolyzes cAMP to inactive fragments. ...
... cell to produce hormone’s effects. • Modulates activity of enzymes present in the cell. • Alters metabolism of the cell. • cAMP inactivated by phosphodiesterase. – Hydrolyzes cAMP to inactive fragments. ...
Protein and Older Adults
... food alone. Nevertheless, animal proteins are a more efficient way to obtain adequate high biological value dietary protein. The amino acid profile of egg is considered to be the standard against which all other proteins are compared. Compared to other high-quality protein sources like meat, poultry ...
... food alone. Nevertheless, animal proteins are a more efficient way to obtain adequate high biological value dietary protein. The amino acid profile of egg is considered to be the standard against which all other proteins are compared. Compared to other high-quality protein sources like meat, poultry ...
Vp-1 Neurospora crassa circadian clock in chemostat culture
... 11 81, E-mail: [email protected] Plant fungal pathogens use nutrients drawn from the plant cells to support their parasitic growth. The enzymatic maceration of plant tissues by the necrotrophic fungi ensures the release of compounds which can be directly assimilated by the fungus and may a ...
... 11 81, E-mail: [email protected] Plant fungal pathogens use nutrients drawn from the plant cells to support their parasitic growth. The enzymatic maceration of plant tissues by the necrotrophic fungi ensures the release of compounds which can be directly assimilated by the fungus and may a ...
Poon, Andy: Predicting Phosphorylation: A critique of the NetPhos program and potential alternatives
... structures. Unfortunately, this particular algorithm produced a high level of false positives (29%-41%). This may not be surprising, given that the data set from which this neural network was nurtured must surely be even smaller than the 414 from PhosphoBase, since it is unlikely that protein struc ...
... structures. Unfortunately, this particular algorithm produced a high level of false positives (29%-41%). This may not be surprising, given that the data set from which this neural network was nurtured must surely be even smaller than the 414 from PhosphoBase, since it is unlikely that protein struc ...
Journal of Bacteriology
... outer membrane antigen group III. For isolation of a clone involved in production of antigen group III of R. leguminosarum bv. viciae strain 248, we made use of the strainspecific reactivity of one of the monoclonal antibodies that recognize the antigen group in this strain. MAb38 appears to recogni ...
... outer membrane antigen group III. For isolation of a clone involved in production of antigen group III of R. leguminosarum bv. viciae strain 248, we made use of the strainspecific reactivity of one of the monoclonal antibodies that recognize the antigen group in this strain. MAb38 appears to recogni ...
Magnetic nanoparticles: applications and cellular uptake
... vesicles. (www.bookworm.org) cell. It shows many clathrin coated pits and vesicles forming on the inner surface of the plasma membrane (John Heuser, J. Cell Biol. 84:560-583, 1980) ...
... vesicles. (www.bookworm.org) cell. It shows many clathrin coated pits and vesicles forming on the inner surface of the plasma membrane (John Heuser, J. Cell Biol. 84:560-583, 1980) ...
Protein translocation pathways across the inner and outer
... amphipathic helix having a hydrophobic surface, is recognized by the binding groove within Tom207, whereas the other half, which is positively-charged, is recognized by the Tom22 of TOM complex. The movement of preproteins through cytosol is associated with cytosolic factors namely chaperones and cy ...
... amphipathic helix having a hydrophobic surface, is recognized by the binding groove within Tom207, whereas the other half, which is positively-charged, is recognized by the Tom22 of TOM complex. The movement of preproteins through cytosol is associated with cytosolic factors namely chaperones and cy ...
Intrinsically Disordered Linker and Plasma Membrane
... defined microenvironments arose. Several means of communication between these organelles evolved to coordinate these processes. One of them is through the formation of direct contact sites between their membranes (1–4). Contact sites were already described in the 1950s and defined as heterologous me ...
... defined microenvironments arose. Several means of communication between these organelles evolved to coordinate these processes. One of them is through the formation of direct contact sites between their membranes (1–4). Contact sites were already described in the 1950s and defined as heterologous me ...
Chapter 3
... enzymes can start separating amino acids. Small intestine: enzymes break down proteins into single amino acids and some small proteins which are absorbed. Amino acids travel in blood to the liver. Amino acid pool provides cells the amino acids they need. If one is not available to build a prot ...
... enzymes can start separating amino acids. Small intestine: enzymes break down proteins into single amino acids and some small proteins which are absorbed. Amino acids travel in blood to the liver. Amino acid pool provides cells the amino acids they need. If one is not available to build a prot ...
Custom RT-qPCR Assays and Panels for any Human, Mouse, or Rat
... 9/8/2015)) and corresponding patent claims outside the United States. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. The right to use this product ...
... 9/8/2015)) and corresponding patent claims outside the United States. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. The right to use this product ...
INVITED SUBMISSION FOR PERSPECTIVE (Revised)
... heterodimeric transmembrane cell adhesion receptor consisting of an α and β subunit which dimerise to form interactions with neighbouring cells or extracellular matrix (ECM) molecules. Integrins are bidirectional signalling molecules. The activation of integrin from a bent low-affinity conformation ...
... heterodimeric transmembrane cell adhesion receptor consisting of an α and β subunit which dimerise to form interactions with neighbouring cells or extracellular matrix (ECM) molecules. Integrins are bidirectional signalling molecules. The activation of integrin from a bent low-affinity conformation ...
Computational design of a pH-sensitive IgG binding
... balance between affinity and the pH dependence of binding, we constructed a library guided by the deep-sequencing data and Rosetta energy calculations and carried out rounds of selection for increased binding affinity at pH 6.5 and 8 (Fig. S5). In the library, a single core substitution L166F was in ...
... balance between affinity and the pH dependence of binding, we constructed a library guided by the deep-sequencing data and Rosetta energy calculations and carried out rounds of selection for increased binding affinity at pH 6.5 and 8 (Fig. S5). In the library, a single core substitution L166F was in ...
Figure 2 - Trace: Tennessee Research and Creative Exchange
... In E. coli, chemotaxis receptors, also called MCPs (for methyl-accepting chemotaxis proteins) sense changes in concentration gradients by making temporal comparisons about the chemical composition of their surroundings (Sourjik and Wingreen, 2012).A functional unit of chemoreceptors is a helical hom ...
... In E. coli, chemotaxis receptors, also called MCPs (for methyl-accepting chemotaxis proteins) sense changes in concentration gradients by making temporal comparisons about the chemical composition of their surroundings (Sourjik and Wingreen, 2012).A functional unit of chemoreceptors is a helical hom ...
chapter 3
... 3. Where stereoisomers of biomolecules are possible, only one is usually found in most organisms; for example, only the L amino acids occur in proteins. What problems would occur if, for example, the amino acids in the body proteins of herbivores were in the L isomer form, whereas the amino acids in ...
... 3. Where stereoisomers of biomolecules are possible, only one is usually found in most organisms; for example, only the L amino acids occur in proteins. What problems would occur if, for example, the amino acids in the body proteins of herbivores were in the L isomer form, whereas the amino acids in ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).