A. Work Accomplished by You and/or Others Cellular biology of
... Cellular biology of protein chaperones Protein molecular chaperones play critical roles in cellular biology. The family of proteins classified as molecular chaperones is large and diverse, but is united by a common characteristic – the ability to form transient yet productive interactions with a bro ...
... Cellular biology of protein chaperones Protein molecular chaperones play critical roles in cellular biology. The family of proteins classified as molecular chaperones is large and diverse, but is united by a common characteristic – the ability to form transient yet productive interactions with a bro ...
Redistribution of membrane proteins between the Golgi apparatus
... redistribution of the JIM84 epitope into the ER of wild type BY2 cells, although some clumps of Golgi material also remained in the cytoplasm (Figure 2n,o). As this antibody recognises a glycan antigen resident on many Golgi proteins (Fitchette et al., 1999; Horsley et al., 1993) the effect of BFA i ...
... redistribution of the JIM84 epitope into the ER of wild type BY2 cells, although some clumps of Golgi material also remained in the cytoplasm (Figure 2n,o). As this antibody recognises a glycan antigen resident on many Golgi proteins (Fitchette et al., 1999; Horsley et al., 1993) the effect of BFA i ...
Responses to Cell Loss Become Restricted as the Supporting Cells
... disruption of F-actin bands in cells within the nonsensory epithelium that extends out from the edge of the sensory macula (Fig. 2B, arrow). Equivalent treatments completely disassembled F-actin bands in MDCK epithelial cells that had formed polarized epithelia, and that effect was reversible as sho ...
... disruption of F-actin bands in cells within the nonsensory epithelium that extends out from the edge of the sensory macula (Fig. 2B, arrow). Equivalent treatments completely disassembled F-actin bands in MDCK epithelial cells that had formed polarized epithelia, and that effect was reversible as sho ...
Smad5 determines murine amnion fate through the
... KEY WORDS: Allantois, Amnion, Bmp, Chimera, PGC, Smad5/Madh5, Stem cell, Tgf ...
... KEY WORDS: Allantois, Amnion, Bmp, Chimera, PGC, Smad5/Madh5, Stem cell, Tgf ...
Involvement of CD14 and Complement Receptors CR3 and CR4 in
... or CR4 cDNA into CHO cells confers upon them the ability to respond to LPS, as evidenced by NF-kB translocation (30, 31). Furthermore, stimulation of neutrophils with LPS in the presence of serum or LBP leads to association between CR3 and CD14, with subsequent dissociation of CD14-CR3 complexes as ...
... or CR4 cDNA into CHO cells confers upon them the ability to respond to LPS, as evidenced by NF-kB translocation (30, 31). Furthermore, stimulation of neutrophils with LPS in the presence of serum or LBP leads to association between CR3 and CD14, with subsequent dissociation of CD14-CR3 complexes as ...
Chato, a KRAB zinc-finger protein, regulates
... the anterior-posterior axis (Yamanaka et al., 2007). However, the importance of convergent extension movements to elongation of other embryonic tissues is not clear, in part owing to a lack of analysis of cell behavior during these stages. Mouse mutants that lack components of the non-canonical Wnt ...
... the anterior-posterior axis (Yamanaka et al., 2007). However, the importance of convergent extension movements to elongation of other embryonic tissues is not clear, in part owing to a lack of analysis of cell behavior during these stages. Mouse mutants that lack components of the non-canonical Wnt ...
Ornithodoros savignyi CHAPTER 2 SIGNALING PATHWAYS REGULATING PROTEIN SECRETION FROM
... (McSwain et al. 1992a). Theophylline, GABA as well as the phorbol esters had no effect when injected individually. Co-injection of the phorbol ester PMA with dopamine and theophylline was inhibitory, indicating an essential role for PKC in eliciting oral secretion. ...
... (McSwain et al. 1992a). Theophylline, GABA as well as the phorbol esters had no effect when injected individually. Co-injection of the phorbol ester PMA with dopamine and theophylline was inhibitory, indicating an essential role for PKC in eliciting oral secretion. ...
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... 2006; Munro and Odell, 2002b). This intercalation involves mediolaterally-biased tractive cell protrusions, as has also been described in Xenopus (Shih and Keller, 1992a; Shih and Keller, 1992b), and may involve a ventrally oriented invagination movement that helps round the notochord in cross secti ...
... 2006; Munro and Odell, 2002b). This intercalation involves mediolaterally-biased tractive cell protrusions, as has also been described in Xenopus (Shih and Keller, 1992a; Shih and Keller, 1992b), and may involve a ventrally oriented invagination movement that helps round the notochord in cross secti ...
Analysis of actin function and organization by a series of mutant
... was isolated from the mutants and nucleotide sequence of the relevant region was determined. From these 67 mutants, we obtained 39 unique mutant alleles. All these alleles had at least one missense mutation in the coding sequence; some alleles had up to four mutations (Table 1). The mutations were ...
... was isolated from the mutants and nucleotide sequence of the relevant region was determined. From these 67 mutants, we obtained 39 unique mutant alleles. All these alleles had at least one missense mutation in the coding sequence; some alleles had up to four mutations (Table 1). The mutations were ...
Early developmental specification of the thyroid gland depends on
... Serls et al., 2005), and tissue explant assays suggest cardiac tissue to be the source of the signals. Moreover, in tissue-explant assays, these FGFs act in a concentration-dependent manner, with high concentrations required for lung, and lower concentrations for liver, induction (Serls et al., 2005 ...
... Serls et al., 2005), and tissue explant assays suggest cardiac tissue to be the source of the signals. Moreover, in tissue-explant assays, these FGFs act in a concentration-dependent manner, with high concentrations required for lung, and lower concentrations for liver, induction (Serls et al., 2005 ...
OPTIMISATION OF CHO CELL GROWTH AND
... A statistical approach was used to identify important culture components for cell growth and LFN-y production. When the concentration of the resulting positive variables was initially increased in culture, improvements of approximately 40% in both of these parameters were achieved; the glycosylation ...
... A statistical approach was used to identify important culture components for cell growth and LFN-y production. When the concentration of the resulting positive variables was initially increased in culture, improvements of approximately 40% in both of these parameters were achieved; the glycosylation ...
In tobacco leaf epidermal cells, the integrity of protein export from
... structures that originate from the Golgi apparatus but that lack coatomer, supporting the model that Arf1 also has a coatomer-independent role for post-Golgi protein transport in plants. When ER to Golgi protein transport is inhibited by mutations that hamper Arf1-GTPase activity without directly di ...
... structures that originate from the Golgi apparatus but that lack coatomer, supporting the model that Arf1 also has a coatomer-independent role for post-Golgi protein transport in plants. When ER to Golgi protein transport is inhibited by mutations that hamper Arf1-GTPase activity without directly di ...
In tobacco leaf epidermal cells, the integrity of protein export from
... structures that originate from the Golgi apparatus but that lack coatomer, supporting the model that Arf1 also has a coatomer-independent role for post-Golgi protein transport in plants. When ER to Golgi protein transport is inhibited by mutations that hamper Arf1-GTPase activity without directly di ...
... structures that originate from the Golgi apparatus but that lack coatomer, supporting the model that Arf1 also has a coatomer-independent role for post-Golgi protein transport in plants. When ER to Golgi protein transport is inhibited by mutations that hamper Arf1-GTPase activity without directly di ...
Response during Sepsis Activation Pathway for the Innate Host
... lacking either the alternative (fD2/2) or classical (C1q2/2) complement activation pathway. Both knockout mice strains showed a significantly reduced survival and increased organ dysfunction when compared with control mice. Surprisingly, fD2/2 mice demonstrated a compensated bacterial clearance capa ...
... lacking either the alternative (fD2/2) or classical (C1q2/2) complement activation pathway. Both knockout mice strains showed a significantly reduced survival and increased organ dysfunction when compared with control mice. Surprisingly, fD2/2 mice demonstrated a compensated bacterial clearance capa ...
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... Yano et al., 2003). These findings suggest that the early step of the gravitropism occurring in the endodermis is impaired in the mutants. They still retain, however, the ability for the latter steps, signal formation in the gravity perceptive cell, intracellular and intercellular signal transductio ...
... Yano et al., 2003). These findings suggest that the early step of the gravitropism occurring in the endodermis is impaired in the mutants. They still retain, however, the ability for the latter steps, signal formation in the gravity perceptive cell, intracellular and intercellular signal transductio ...
- Wiley Online Library
... RNAi of drr-2 increases the lifespan of wild-type animals by 27% but fails to further extend the lifespan of eat-2 (ad1116) mutants. Furthermore, RNAi of drr-2 extends lifespan in a daf16-independent manner, as observed in the DR animals. The mRNA levels of drr-2 were also found to be significantly ...
... RNAi of drr-2 increases the lifespan of wild-type animals by 27% but fails to further extend the lifespan of eat-2 (ad1116) mutants. Furthermore, RNAi of drr-2 extends lifespan in a daf16-independent manner, as observed in the DR animals. The mRNA levels of drr-2 were also found to be significantly ...
Autophagy regulation by nutrient signaling
... negatively regulating mTORC1 through either AMPK-mediated phosphorylation of mTORC1 or activation of the upstream repressor TSC. Limited oxygen also upregulates hypoxia-responsive genes, which are capable of suppressing mTORC1 signaling through the activation of TSC or inhibition of Rheb. Amino-acid ...
... negatively regulating mTORC1 through either AMPK-mediated phosphorylation of mTORC1 or activation of the upstream repressor TSC. Limited oxygen also upregulates hypoxia-responsive genes, which are capable of suppressing mTORC1 signaling through the activation of TSC or inhibition of Rheb. Amino-acid ...