Classification of plant-pathogenic mycoplasma
... Based on Southern hybridization with a DNA fragment of an MLO associated with aster yellows, a differentiation between organisms inducing decline symptoms and those causing floral virescence has been proposed (Kuske et al., 1991). Although several organisms have been differentiated using these metho ...
... Based on Southern hybridization with a DNA fragment of an MLO associated with aster yellows, a differentiation between organisms inducing decline symptoms and those causing floral virescence has been proposed (Kuske et al., 1991). Although several organisms have been differentiated using these metho ...
Fragaria multicipita - [email protected] of Nebraska
... F2n/R2. Following RFLP analysis using thirteen restriction enzymes including TaqI, the total of DNA fragment sizes in each RFLP pattern agreed with the 1.2 kbp size expected for 16S rDNA amplified in PCR primed by F2n/R2 and were the same as noted previously (Jomantiene et al. 1998 a) (Fig. 1, mF2/R ...
... F2n/R2. Following RFLP analysis using thirteen restriction enzymes including TaqI, the total of DNA fragment sizes in each RFLP pattern agreed with the 1.2 kbp size expected for 16S rDNA amplified in PCR primed by F2n/R2 and were the same as noted previously (Jomantiene et al. 1998 a) (Fig. 1, mF2/R ...
Purification and amino acid sequence of a bacteriocins produced by
... to Abp 118 beta produced by Lb. salivarius UCC118. In addition, Lb. salivarius UCC118 produced 2-peptide bacteriocin, which was Abp 118 alpha and beta. Based on the partial amino acid sequences of Abp 118 beta, specific primers were designed from nucleotide sequences according to data from GenBank. ...
... to Abp 118 beta produced by Lb. salivarius UCC118. In addition, Lb. salivarius UCC118 produced 2-peptide bacteriocin, which was Abp 118 alpha and beta. Based on the partial amino acid sequences of Abp 118 beta, specific primers were designed from nucleotide sequences according to data from GenBank. ...
Design and Analysis of Crossover Trials
... ♠ Obviously, randomization is very important if the crossover design is not uniform within sequences because the underlying assumption is that the sequence effect is negligible. ♠ Randomization is important in crossover trials even if the design is uniform within sequences because biases could resul ...
... ♠ Obviously, randomization is very important if the crossover design is not uniform within sequences because the underlying assumption is that the sequence effect is negligible. ♠ Randomization is important in crossover trials even if the design is uniform within sequences because biases could resul ...
Comparison of FIESTA and gadolinium-enhanced T1
... ear to induce nystagmus) investigate the integrity of the oculovestibular reflex, which is mediated by the superior vestibular nerve.17 Abnormal caloric tests may be a result of an AS of this nerve, however, caloric tests may be normal if the AS is associated with the inferior branch of the vestibul ...
... ear to induce nystagmus) investigate the integrity of the oculovestibular reflex, which is mediated by the superior vestibular nerve.17 Abnormal caloric tests may be a result of an AS of this nerve, however, caloric tests may be normal if the AS is associated with the inferior branch of the vestibul ...
Carpal Tunnel Release
... There are more than 120 different types of brain tumors. Brain tumors are often assigned different grades, ranging from a Grade I (least malignant) to Grade IV (most malignant). It is important to note that non-malignant, or benign, brain tumors can be just as difficult to treat as malignant bra ...
... There are more than 120 different types of brain tumors. Brain tumors are often assigned different grades, ranging from a Grade I (least malignant) to Grade IV (most malignant). It is important to note that non-malignant, or benign, brain tumors can be just as difficult to treat as malignant bra ...
Answers #2
... available for the next step- I think those other ligation events were either ignored or underestimated. Several answers discussed using adapters or linkers. I don’t understand why because the question already presents you with fragments containing ideal end-points. Several answers suggested using SL ...
... available for the next step- I think those other ligation events were either ignored or underestimated. Several answers discussed using adapters or linkers. I don’t understand why because the question already presents you with fragments containing ideal end-points. Several answers suggested using SL ...
Table SI. Primers used for creation of the PHAC1co and
... Transformants were selected on SD medium. Correct integration of the UGT1co expression cassette at the UGTB1 locus was controlled by two yeast colony PCR’s. Primers KIUGT1 CtrlFor2 and KIUGT1CtrlRev were used to check 3’ cross-over while primers Ura3endFor and GTII+239Rev were used to check cross-ov ...
... Transformants were selected on SD medium. Correct integration of the UGT1co expression cassette at the UGTB1 locus was controlled by two yeast colony PCR’s. Primers KIUGT1 CtrlFor2 and KIUGT1CtrlRev were used to check 3’ cross-over while primers Ura3endFor and GTII+239Rev were used to check cross-ov ...
GI Endoscopic Procedures Operative Sequence - A
... A surgical procedure to treat various diseases of the thyroid such as hyperthyroidism and cancer that can not be treated with chemotherapy. Hyperthyroidism - excessive functionality of the thyroid gland marked by increased metabolic rate, enlargement of the thyroid gland, rapid heart rate, high bloo ...
... A surgical procedure to treat various diseases of the thyroid such as hyperthyroidism and cancer that can not be treated with chemotherapy. Hyperthyroidism - excessive functionality of the thyroid gland marked by increased metabolic rate, enlargement of the thyroid gland, rapid heart rate, high bloo ...
Series Length Used during Trend Analysis Affects Sensitivity to
... open-angle glaucoma.1,2 A second phase of the trial in which all participants were offered IOP-lowering medication, demonstrated that the rate of conversion to primary open-angle glaucoma (POAG) was lower in those participants who had been randomized initially to treatment, that is that delaying tre ...
... open-angle glaucoma.1,2 A second phase of the trial in which all participants were offered IOP-lowering medication, demonstrated that the rate of conversion to primary open-angle glaucoma (POAG) was lower in those participants who had been randomized initially to treatment, that is that delaying tre ...
Dell`Orphano: SNP discovery
... • 55 were discarded by computer analysis (filters). • The oligonucleotide selection program (osp) was used to design primers to amplify the 98 remaining SNPs. • 30 SNPs were in regions with no suitable amplimers. • In all, 44 STSs were developed to amplify the remaining 68 SNPs, with 16 STSs contain ...
... • 55 were discarded by computer analysis (filters). • The oligonucleotide selection program (osp) was used to design primers to amplify the 98 remaining SNPs. • 30 SNPs were in regions with no suitable amplimers. • In all, 44 STSs were developed to amplify the remaining 68 SNPs, with 16 STSs contain ...
PDF
... † Various different LGE-MRI sequences are capable of identifying this VA substrate. However, no direct comparison has been made between theses sequences. It could be important to standardized image acquisition and to optimize VA substrate identification. † This study demonstrates that 3D sequences p ...
... † Various different LGE-MRI sequences are capable of identifying this VA substrate. However, no direct comparison has been made between theses sequences. It could be important to standardized image acquisition and to optimize VA substrate identification. † This study demonstrates that 3D sequences p ...
Additional file 5
... B) Probes exhibiting true signals were subdivided into those showing (red) signals only in the Bp K96243 hybridization, (purple) signals in both the BpK96243 and Bp22 hybridization, and (blue) signals only in the Bp22 hybridization. Y-axis : Probe abundance (log2 scale). X-axis : Hybridization rati ...
... B) Probes exhibiting true signals were subdivided into those showing (red) signals only in the Bp K96243 hybridization, (purple) signals in both the BpK96243 and Bp22 hybridization, and (blue) signals only in the Bp22 hybridization. Y-axis : Probe abundance (log2 scale). X-axis : Hybridization rati ...
ASE using Solexa Protocol
... 2) Design gene specific 18-20bp annealing primers as follows: forward primer flanking the 5' end of the SNP such that the base immediately following the 3’ end of the primer is the SNP, the second 200-300bp's downstream from the SNP. 3) Check primer design and verify that no additional SNP's occur w ...
... 2) Design gene specific 18-20bp annealing primers as follows: forward primer flanking the 5' end of the SNP such that the base immediately following the 3’ end of the primer is the SNP, the second 200-300bp's downstream from the SNP. 3) Check primer design and verify that no additional SNP's occur w ...
L 17 _PCR
... 2) Anneal: cool template/primer mix to appropriate temperature (50-70 C) so that primers can anneal (hybridize) with template 3) Extend: shift to temp at which DNA polymerase functions best (72 C for Taq) Each set of three steps is one cycle; each cycle doubles the amount of DNA product. Generally a ...
... 2) Anneal: cool template/primer mix to appropriate temperature (50-70 C) so that primers can anneal (hybridize) with template 3) Extend: shift to temp at which DNA polymerase functions best (72 C for Taq) Each set of three steps is one cycle; each cycle doubles the amount of DNA product. Generally a ...
2008 BSHG newesletter 01
... performed in a single run. With conventional Sanger sequencing by capillary electrophoresis up to 384 sequences (more usually 96) can be generated in a single run. In contrast, the new technologies are capable of performing hundreds of thousands to many 10s of millions of analyses in parallel, hence ...
... performed in a single run. With conventional Sanger sequencing by capillary electrophoresis up to 384 sequences (more usually 96) can be generated in a single run. In contrast, the new technologies are capable of performing hundreds of thousands to many 10s of millions of analyses in parallel, hence ...
mouse. However, some technical and prac-
... genes provides an efficient way to generate proteins with new traits1,2. The resulting molecules are very different, at least in sequence, from those that might be obtained by more local searches of protein space, for example by random mutagenesis. The DNA shuffling method, which relies on homologou ...
... genes provides an efficient way to generate proteins with new traits1,2. The resulting molecules are very different, at least in sequence, from those that might be obtained by more local searches of protein space, for example by random mutagenesis. The DNA shuffling method, which relies on homologou ...
Stefan Zimmermann1,C. Seyboldt 2, F. Günther1, J
... compared to a subtyping dendrogram generated by MALDI-TOF mass spectrometry using Biotyper software. Random amplified polymorphic DNA (RAPD)-PCR was also performed. As RAPD-PCR is often used for subtyping Gram-negative bacteria, while the more recent method of Fourier transformation-Infrared Spectro ...
... compared to a subtyping dendrogram generated by MALDI-TOF mass spectrometry using Biotyper software. Random amplified polymorphic DNA (RAPD)-PCR was also performed. As RAPD-PCR is often used for subtyping Gram-negative bacteria, while the more recent method of Fourier transformation-Infrared Spectro ...
Supplementary material 1 grimalt
... initially calculated as close to 100%; therefore EAct and ETG values were set at 2 for all further calculations. The suitability of ß-Actin as reference gene for these samples has been tested previously2. Results are expressed as copies of dio2 or CYP1A mRNA per 1000 copies of ß-actin mRNA. A typica ...
... initially calculated as close to 100%; therefore EAct and ETG values were set at 2 for all further calculations. The suitability of ß-Actin as reference gene for these samples has been tested previously2. Results are expressed as copies of dio2 or CYP1A mRNA per 1000 copies of ß-actin mRNA. A typica ...
Pictorial Essay: Pulmonary Imaging for Children
... (TSE) sequence, a true fast imaging with steady-state free precession (TrueFISP) sequence, dynamic volume interpolated breath-hold examination (VIBE) sequences, and a T1-weighted fast low angle shot (FLASH) 2D sequence. However in all of these sequences we have quite a few, especially motion artifac ...
... (TSE) sequence, a true fast imaging with steady-state free precession (TrueFISP) sequence, dynamic volume interpolated breath-hold examination (VIBE) sequences, and a T1-weighted fast low angle shot (FLASH) 2D sequence. However in all of these sequences we have quite a few, especially motion artifac ...
RNA sequencing - Bioinformatics.ca
... – If the gene is expressed but only from the wild type allele, this might suggest loss-of-function (haploinsufficiency) – If the mutant allele itself is expressed, this might suggest a candidate ...
... – If the gene is expressed but only from the wild type allele, this might suggest loss-of-function (haploinsufficiency) – If the mutant allele itself is expressed, this might suggest a candidate ...
What are the methods and approaches used to identify and
... How do we Know if There is an Insert? • Genotyping provides evidence for a T-DNA insert • Genotyping is done by PCR amplification of the DNA sequence of our gene with our gene-specific primers along with a primer for the T-DNA insert • We know of two ways to genotype 1. Multiplex Reaction 2. Separa ...
... How do we Know if There is an Insert? • Genotyping provides evidence for a T-DNA insert • Genotyping is done by PCR amplification of the DNA sequence of our gene with our gene-specific primers along with a primer for the T-DNA insert • We know of two ways to genotype 1. Multiplex Reaction 2. Separa ...
Supplementary Information
... 3. Quantitative RT-PCR. Patient muscle RNA was prepared from ~100 mg of vastus lateralis biopsy material by homogenising in 1 ml of QIAzol (RNA extraction kit, QIAGEN) and subjecting to RNA extraction according to the manufacturers’ instructions. Human muscle RNA purchased from Ambion was used as a ...
... 3. Quantitative RT-PCR. Patient muscle RNA was prepared from ~100 mg of vastus lateralis biopsy material by homogenising in 1 ml of QIAzol (RNA extraction kit, QIAGEN) and subjecting to RNA extraction according to the manufacturers’ instructions. Human muscle RNA purchased from Ambion was used as a ...
Detection of Transcription Factor Binding Sites
... Image from: http://www.ideacenter.org/contentmgr/showdetails.php/id/954 ...
... Image from: http://www.ideacenter.org/contentmgr/showdetails.php/id/954 ...
