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Principles of TEM image formation Principles of TEM image
Principles of TEM image formation Principles of TEM image

... phase variations over the plane surface. T(x,y) = A0exp[iφ(x,y)], for simplicity : A0 = 1 Assuming that the object is thin and phase shift φ is small The approximation of the emerged wave might be described as ...
Compiled questions (docx 32 kB)
Compiled questions (docx 32 kB)

... 1. Any method used in analytical microscopy needs a calibration, which includes answering following question: what is the functional relation between the concentration of the species being measured and the signal that the microscope measures at a given excitation intensity? For usual confocal laser ...
SPECTRAL ANALYSIS
SPECTRAL ANALYSIS

... intensities of any lines which had intensities less than 4.5 (and may even have been unobserved) in the first sets of data. Since the GAIN value is 10, you must divide all your intensity values by 10. You should be able to obtain data for a total of 5 or 6 spectral lines of mercury. ...
living with the lab
living with the lab

... This sensor detects infrared (IR) light. However, to avoid detecting the IR component of regular sunlight or light from other stray IR sources, the sensor only looks for light coming in at 38 kHz. ...
Document
Document

... • Incident ray parallel to P.A will reflect through focus • Incident ray through focus will reflect parallel to P.A. • Incident ray through Center of Curv. will reflect back on self – Don’t forget regular incidence=reflection from vertex ...
L32
L32

... Homer’s image ...
4.5 Forming the Perfect Image Is a Tall Order Ideally we would like
4.5 Forming the Perfect Image Is a Tall Order Ideally we would like

... Ideally we would like an image formed by the optics to be an exact copy of the scene in every imaginable way. To replicate the scene, we would need to record the three-dimensional aspects of the scene with the proper color and brightness. Then, there is the changing nature of the scene over time tha ...
HW #8 Solutions
HW #8 Solutions

... your image has the left,right sides of your face similarly swapped, which is not how others see you (or how you appear in a photograph). Chapter 17 Exercises 23 Estimate the time needed for light to travel the distance across the continental US. Take the distance across the US to be about 3000 miles ...
Absorption Measurements on PC1
Absorption Measurements on PC1

... which asks to select the 2nd blank transmission spectrum. Select the spectrum by double-clicking on it and then click OK. This will produce the background corrected absorption spectrum. Note: Both the sample and blank data files require smoothing with Vinci Analysis before the actual absorption spec ...
Single Pixel Cameras wall panels
Single Pixel Cameras wall panels

... extensively throughout the research and development process. The text and diagrams show the experimental layout and mathematical equations used in computational imaging techniques, as well as other writings from different experiments ...
Nano-optical Imaging using Scattering Scanning Near-Field Optical Microscopy
Nano-optical Imaging using Scattering Scanning Near-Field Optical Microscopy

... We investigate a nano-imaging technique, known as scattering scanning near-field optical microscopy (s-SNOM) and image several different materials using said technique. We report our data provide potential paths for future work. I. INTRODUCTION Scientists have long studied optical spectroscopy due t ...
pp. 273
pp. 273

... disturbing, while in Fig. 11 it is virtually undetectable. One technique to suppress the crosstalk associated with nonlinear storage is to control the carrier's transmission geometry. The development of this nonlinear multiple image method will be the subject of another paper. ...
L05D - Clarkson University
L05D - Clarkson University

... These Kanji characters represent the word “atom.” ...
39 Steps
39 Steps

... and might include “tricks-of-the-trade”, novel methods of specimen preparation, practices of image collection, tips on the digital manipulation and publication of images and historical perspectives. ...
Depth-of-Focus in Microscopy
Depth-of-Focus in Microscopy

... there is an urgent need for instrumentation to count hundreds to thousands of cells to assess, for example, aneuploidy, trisomies, and minimum residual disease (MRD). To develop the appropriate instrumentation we have had to examine a number of problems in depth. One of these concerns an accurate de ...
Resolution questions with solutions
Resolution questions with solutions

... Labels should include: maxima 1; maxima 2; minima 2 and minima 1 A note saying” just resolved” or similar Note that a well-labelled and annotated diagram could receive up to 3 max ...
light reflection plane mirror
light reflection plane mirror

... Normal – a dashed line drawn perpendicular to the mirror at the point of reflection (an imaginary line) Incident Ray – incoming ray of light Reflected Ray – ray of light that bounces off an object Angle of Incidence (θi) – angle between the incident ray and the normal Angle of Reflection (θr) – ang ...
Modellistica 3D di Componenti Cellulari
Modellistica 3D di Componenti Cellulari

... Crown & Flint glass Crown glass Is a type of glass used in lenses and other optical components. It has relatively low refractive index (≈1.52) and low dispersion (with Abbe numbers around 60). Crown glass is produced from alkali-lime (RCH) silicates containing approximately 10% potassium oxide and ...
The excitation mechanism excitation mechanism
The excitation mechanism excitation mechanism

... with the gas molecules and transfer energy to them. Thus, the gas molecules are raised to excited state. Higher voltage is required to start the electrical discharge in the tube than to keep the discharge. Thus, a preliminary high voltage pulse is applied for initial discharge, and then the voltage ...
Microscopy
Microscopy

... light is invisible to the human eye, so the image must be recorded on a photographic plate or fluorescent screen. Because this light is absorbed by glass, all lenses must be made of quartz, such microscopes are two expensive for routine use. ...
Problem Set 1 (due 2/21/06)
Problem Set 1 (due 2/21/06)

... modes of water are relatively small, and aqueous samples may be analyzed (water absorbs strongly in the IR). This allows analysis of a wide range of chemical and biological samples (even in vivo). Raman measures vibrational mode energies, but uses a visible wavelength light source. Optics and detect ...
Document
Document

... By the end of this part of the course, you should be able to: •Understand interaction between light and matter (absorbance, excitation, emission, luminescence,fluorescence, phosphorescence) •Describe the main components of a spectrophotometer, (sources, monochromators, detectors, interferometer, gr ...
Optics supplemental notess
Optics supplemental notess

... Where is the focal point (or is there one)? Will the image be magnified or reduced in size? Will the image be upside down or right side up? Will the image be inverted left or right? ...
Lecture 1. Introduction. Nature of light, geometric optics.
Lecture 1. Introduction. Nature of light, geometric optics.

... • S is distance from the object; S’ is distance from the image • Sign conventions: m = positive for inverted image; negative for upright • Sign conventions: f = positive for converging lens; negative for diverging ...
Document
Document

... Why is TIRF interesting? Provides high resolution along z – overcomes wide-field limit Limitation: only measures the surface, Still important for various applications. ...
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Johan Sebastiaan Ploem

.Johan Sebastiaan Ploem (born 25 August 1927, Sawahlunto) is a Dutch microscopist and digital artist, who made significant contribution to the field of fluorescence microscopy.
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