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Background concepts for sequence analysis Ana, homo
Background concepts for sequence analysis Ana, homo

... The claim that two sequences are homolog thus results from an inference, based on some evolutionary scenario (rate of mutation, level of similarity, !). The inference of homology is always attached to some risk of false positive. Evolutionary models allow to estimate this risk, as we shall see. ...
Genomic structure and promoter analysis of pathogen-induced genes from
Genomic structure and promoter analysis of pathogen-induced genes from

... from ATG to the stop codon, including intronic sequences, revealed both intra- and interpopulation sequence heterogeneity. A nucleotide identity from 88.0 to 96.3% was observed among repat1 clones, and from 86.3 to 98.4% among repat2 clones. Amplicons of these clones also showed length variability, ...
Supplementary Materials and Methods
Supplementary Materials and Methods

... analysis as the previous set (TREE-PUZZLE, PHYML, etc.) and trees with the same topology and similar branch lengths to those seen with the previous alignment were produced. ...
Pan-genomics: Unmasking the gene diversity hidden in the bacteria
Pan-genomics: Unmasking the gene diversity hidden in the bacteria

... huge amounts of bacteria diversity out there, for some environments we only knew about 1% of the estimated diversity in part because of the difficulties to culture bacteria in Petri dishes [8]. Parallel to the advancement of sequencing technologies the sequencing of environmental DNA and thus the ge ...
Lazarus and doppelganger genes
Lazarus and doppelganger genes

... • Highest proportion of HGT genes are in bdelloid rotifers • 10% of transcripts ...
HW3 - solutions
HW3 - solutions

... a. Explain in one-two sentences what does each ontology refer to and give one example for each. Cellular component – annotations of genes by the location of the protein they code to in the cell. For example – cell membrane. Biological process – annotations of genes by the pathway or process the prot ...
Molecular genetic basis of porcine histo
Molecular genetic basis of porcine histo

... Poly A⫹ RNA was prepared from group A porcine submaxillary glands and used for RT-PCR of a putative porcine A transferase complementary DNA using 2 primers: FY-530 (5⬘-CTCCAGGCACCTGGCTTG) and FY-531 (5⬘-CCCTCCTCCTGTTCGTCG). The sequences in these primers corresponded to the sequences in the 5⬘ UTR a ...
Promoter-trapping in Saccharomyces cerevisiae
Promoter-trapping in Saccharomyces cerevisiae

Documentation of MetaMine
Documentation of MetaMine

... Using the default parameters the user will obtain a maximal amount of redundance-free gene patterns, excluding patters below a length of three genes. To focus on more frequent patterns the user can increase the parameter quorum. Increasing the parameter minimal pattern length results in a lower numb ...
Gene Maps
Gene Maps

... KEY CONCEPT: •What structures actually assort independently? ...
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... At this point go to Step 3 and Click on GBrowse (green rectangle) to get the gene sequence from the short chromosome and follow the same procedure upto Step 9. If possible pick sgRNA candidates that are identical for long and short chromosome genes. Now, make sure your choice meets inclusion and exc ...
14-3 Human Molecular Genetics
14-3 Human Molecular Genetics

... The Human Genome Project is an ongoing effort to analyze the human DNA sequence. In 1990, scientists in the United States and other countries began the Human Genome Project. In June 2000, a working copy of the human genome was essentially complete. It has sequenced all of the DNA base pairs and chro ...
Chapter 26 - RNA Metabolism
Chapter 26 - RNA Metabolism

... catalyzes a processive elongation of RNA chain (about 17 bp at a time) transcription bubble • Mechanism of elongation reaction almost identical to that for DNA polymerase • Incoming ribonucleotide triphosphates (RTPs) form correct H bonds to template • New phosphodiester bond formed, PPi released • ...
1st_pres_Geneprediction
1st_pres_Geneprediction

... Functional elements -> Regulatory regions ...
Align the DNA sequences
Align the DNA sequences

... DNA SEQUENCE RESOURCES: The National Center for Biotechnology Information (NCBI)Established in 1988 as a national resource for molecular biology information, NCBI creates public databases, conducts research in computational biology, develops software tools for analyzing genome data, and disseminate ...
Potential for Selection of Beneficial Traits in Swine with Site
Potential for Selection of Beneficial Traits in Swine with Site

... percentage of the embryos developed to the blastocyst stage. Additionally, when we evaluated the sequences, many of our embryos had only a one-codon deletion. Accordingly, we moved on to CRISPRs because we had that technology operational at the same time, and, it seemed to work better in our hands. ...
Untitled
Untitled

... sequencing. The other refers to the machinery inside a cell reading a gene when it is switched on, making a copy of the information within it in the form of a message called RNA. This process is more formally known as transcription, and I will talk about genes being transcribed or read into RNA. Hop ...
Analysis of Flanking Sequences from Dissociation
Analysis of Flanking Sequences from Dissociation

... 1996a, 1996b). Each NOR occupies 3.5 to 4.0 Mb and consists of tandemly repeated rRNA gene clusters. The nucleolus is organized around the NORs during interphase and is associated with very active transcription of ribosomal genes by RNA polymerase I. The increasing frequency of insertions into the N ...
Analysis of Flanking Sequences from Dissociation
Analysis of Flanking Sequences from Dissociation

... 1996a, 1996b). Each NOR occupies 3.5 to 4.0 Mb and consists of tandemly repeated rRNA gene clusters. The nucleolus is organized around the NORs during interphase and is associated with very active transcription of ribosomal genes by RNA polymerase I. The increasing frequency of insertions into the N ...
DOCX 60 KB - Office of the Gene Technology Regulator
DOCX 60 KB - Office of the Gene Technology Regulator

... Up to 50 lines of GM wheat are proposed for release. Each contains one of 15 genes encoding proteins expected to enhance drought tolerance by regulating gene expression or modulating biochemical and signal transduction pathways in the wheat plants. The genes are derived from the plants thale cress ( ...
Generalized-HMMs - Center for Bioinformatics and
Generalized-HMMs - Center for Bioinformatics and

... • Comparative (homology) based gene finders. These align genomic sequences from different species and use the alignments to guide the gene predictions (e.g. TWAIN, SLAM, ...
News Features Human genome sequences — a potential treasure
News Features Human genome sequences — a potential treasure

... Sequencing, genetics and medicine A genome comprises essentially four main types of molecules, or bases — adenine, thiamine, guanine and cytosine — arranged in pairs in a double helical structure. There are 3 billion base pairs and their order carries the instructions to make a human being. Of the e ...
Chloroplast genes for components of the ATP synthase complex
Chloroplast genes for components of the ATP synthase complex

... 50 kbp apart. The genes for fl and E subunits are close to, and transcribed divergently from, the gene for the large subunit of ribulose bisphosphate carboxylase (Koller et al., 1982; Oishi & Tewari, 1983). The genes for a and subunit I11 are 2 kbp apart, and are transcribed in the same direction as ...
Genomics – the future of healthcare and medicine
Genomics – the future of healthcare and medicine

... enable patients to learn their genetic risks for disease and also help healthcare professionals to diagnose disease • At least 350 biotechnology-based products resulting from the Human Genome Project are currently in clinical trials • Much work still remains to be done. Despite many important gene ...
control of the drosophila body pattern
control of the drosophila body pattern

... responsible for segmental identity in the head and anterior thorax, whereas the BX-C complex (Bithorax complex) is responsible for segmental identity in the posterior thorax and abdomen. Homeosis or homeotic transformation is the development of one body part with the phenotype of another. Three exam ...
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Transposable element



A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.
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