Chapter 11
... Replication • On the lagging strand, growing in the other direction, DNA is made in the 5’-to-3’ direction but synthesis is discontinuous: • DNA is added as short fragments to primers, then the polymerase skips past the 5’ end to make the next fragment. Review Figures 11.16, 11.17 and 11.18 ...
... Replication • On the lagging strand, growing in the other direction, DNA is made in the 5’-to-3’ direction but synthesis is discontinuous: • DNA is added as short fragments to primers, then the polymerase skips past the 5’ end to make the next fragment. Review Figures 11.16, 11.17 and 11.18 ...
Molecular pathogenesis of feline leukemia virus
... University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan ...
... University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan ...
ppt
... Evidence That Viral DNA Can Program Cells • Additional evidence for DNA as the genetic material came from studies of a virus that infects bacteria • Viruses that infect bacteria, bacteriophages are widely used as tools by researchers in molecular genetics Phage head ...
... Evidence That Viral DNA Can Program Cells • Additional evidence for DNA as the genetic material came from studies of a virus that infects bacteria • Viruses that infect bacteria, bacteriophages are widely used as tools by researchers in molecular genetics Phage head ...
Small hive beetle, Aethina tumida, as a potential biological vector of
... Elzen, 2004; Neumann and Ellis, 2008) and a severe pest in some regions (USA: Elzen et al., 2000; Australia: Spiewok et al., 2007). Freeflying SHB adults invade host colonies over several kilometers, mate and reproduce within hives (Neumann and Elzen, 2004; Spiewok et al., 2008). Although the pest s ...
... Elzen, 2004; Neumann and Ellis, 2008) and a severe pest in some regions (USA: Elzen et al., 2000; Australia: Spiewok et al., 2007). Freeflying SHB adults invade host colonies over several kilometers, mate and reproduce within hives (Neumann and Elzen, 2004; Spiewok et al., 2008). Although the pest s ...
Redalyc.Molecular Epidemiology and Evolution of Avian Infectious
... Mutation and recombination processes are involved in the genetic and phenotypic variations of RNA viruses, leading to the emergence of new variant strains, and give rise to virus population diversity to be modeled by the host, particularly by the immune system, as occurred with infectious bronchitis ...
... Mutation and recombination processes are involved in the genetic and phenotypic variations of RNA viruses, leading to the emergence of new variant strains, and give rise to virus population diversity to be modeled by the host, particularly by the immune system, as occurred with infectious bronchitis ...
CIRCULAR DNA MOLECULES IN THE GENUS
... to determine the circularity of the Drosophila DNA's. EBr, (Boots Pure Drug Co., Ltd., Nottingham, England) was used at a concentration of 100 pg per ml CsCl, p = 1.580g cm-3. DNA samples, 50-150 p g , were centrifuged in 3 ml volumes of EBr-CsC1 in cellulose nitrate tubes overlaid with mineral oil ...
... to determine the circularity of the Drosophila DNA's. EBr, (Boots Pure Drug Co., Ltd., Nottingham, England) was used at a concentration of 100 pg per ml CsCl, p = 1.580g cm-3. DNA samples, 50-150 p g , were centrifuged in 3 ml volumes of EBr-CsC1 in cellulose nitrate tubes overlaid with mineral oil ...
Proof corrections should be returned in one communication to Justin
... homologous recombination is to assemble these nucleoprotein filaments so the homology search can begin. In vivo, the ssDNA substrate for filament formation is coated with singlestranded DNA-binding protein (bacterial SSB, phage T4 Gp32, or eukaryotic RPA) as soon as it is generated. Although the bin ...
... homologous recombination is to assemble these nucleoprotein filaments so the homology search can begin. In vivo, the ssDNA substrate for filament formation is coated with singlestranded DNA-binding protein (bacterial SSB, phage T4 Gp32, or eukaryotic RPA) as soon as it is generated. Although the bin ...
Communication: Formation of Knots in Partially Replicated DNA
... ¯anking regions, which are indicated by asterisks). To correctly recognise the knot type it is necessary to determine at each crossing which segment passes under and which passes over. The more complicated the knot, the higher the chance that at least one of the crossings would be problematic in its ...
... ¯anking regions, which are indicated by asterisks). To correctly recognise the knot type it is necessary to determine at each crossing which segment passes under and which passes over. The more complicated the knot, the higher the chance that at least one of the crossings would be problematic in its ...
Processivity of DNA polymerases: two mechanisms, one goal
... The mechanism adopted by bacteriophage T7 to replicate its DNA is more economic than that used by cellular organisms. In T7 only three proteins are needed for processive DNA elongation: the polymerase (gp5), E. coli thioredoxin, and a single-stranded DNA-binding protein (SSB; gene 2.5 protein). In T ...
... The mechanism adopted by bacteriophage T7 to replicate its DNA is more economic than that used by cellular organisms. In T7 only three proteins are needed for processive DNA elongation: the polymerase (gp5), E. coli thioredoxin, and a single-stranded DNA-binding protein (SSB; gene 2.5 protein). In T ...
The Living World
... The viral enzyme reverse transcriptase synthesizes double-stranded DNA from the single-stranded RNA The enzyme does not proofread so many mutations are introduced The double-stranded DNA is integrated into the host cell’s genome In all of this, no lasting damage is done to the host cell Copyright ©T ...
... The viral enzyme reverse transcriptase synthesizes double-stranded DNA from the single-stranded RNA The enzyme does not proofread so many mutations are introduced The double-stranded DNA is integrated into the host cell’s genome In all of this, no lasting damage is done to the host cell Copyright ©T ...
Identification of human herpesvirus 6 uracil
... uracil-DNA glycosylaseactivitywas definedas the amount of enzyme required to release 1 pmol of uracil as acid-solublematerial per rain. t NO, not determined. described in Methods. The data presented in Table 2 summarize the results of the uracil-DNA glycosylase assay. When [3H]dUTP-labelled D N A wa ...
... uracil-DNA glycosylaseactivitywas definedas the amount of enzyme required to release 1 pmol of uracil as acid-solublematerial per rain. t NO, not determined. described in Methods. The data presented in Table 2 summarize the results of the uracil-DNA glycosylase assay. When [3H]dUTP-labelled D N A wa ...
2000 Genome Biology paper
... X-alignment is highly statistically significant (Table 2). (c) Chlamydia trachomatis versus C. pneumoniae, top matches. Only the best match for each C. trachomatis ORF is shown. The position of the origins (R) and termini (T) of replication are slightly shifted to see the inversions better. This pat ...
... X-alignment is highly statistically significant (Table 2). (c) Chlamydia trachomatis versus C. pneumoniae, top matches. Only the best match for each C. trachomatis ORF is shown. The position of the origins (R) and termini (T) of replication are slightly shifted to see the inversions better. This pat ...
Detection of two novel porcine herpesviruses with high similarity to
... Evidence for the existence of porcine gammaherpesviruses was obtained by PCR and sequence analysis. Initially, samples of peripheral blood mononuclear cells (PBMC), spleens, lungs, kidneys and livers of pigs from Germany and Spain were tested with a PCR assay which targets conserved regions of the h ...
... Evidence for the existence of porcine gammaherpesviruses was obtained by PCR and sequence analysis. Initially, samples of peripheral blood mononuclear cells (PBMC), spleens, lungs, kidneys and livers of pigs from Germany and Spain were tested with a PCR assay which targets conserved regions of the h ...
replication v 2015_21
... – Single stranded binding proteins – After the helix has opened it is prevented from reannealing by the action of these proteins – These proteins stabilize single stranded DNA - Single-strand-binding proteins help to stabilize the unwound strands. – Topoisomerase II relieves strain on the double hel ...
... – Single stranded binding proteins – After the helix has opened it is prevented from reannealing by the action of these proteins – These proteins stabilize single stranded DNA - Single-strand-binding proteins help to stabilize the unwound strands. – Topoisomerase II relieves strain on the double hel ...
Molecular Classification of Infectious Laryngotracheitis Virus from
... The molecular classification of the infectious laryngotracheitis virus from chicken embryo origin (CEO) vaccine, tissue culture origin (TCO) vaccine and field isolates by the sequence of ICP4 genes from CEO and TCO vaccines in the GenBank was performed for the different sequence analysis. The sequen ...
... The molecular classification of the infectious laryngotracheitis virus from chicken embryo origin (CEO) vaccine, tissue culture origin (TCO) vaccine and field isolates by the sequence of ICP4 genes from CEO and TCO vaccines in the GenBank was performed for the different sequence analysis. The sequen ...
Quantifying relative within-host replication fitness in influenza virus
... Hauge et al., 2009; Meijer et al., 2009). These resistant viruses spread globally and replaced wild-type H1N1 during 2008 (Centers for Disease Control and Prevention (CDC), 2009; Dharan et al., 2009; Hurt et al., 2009; Moscona, 2009), largely independently of the use of oseltamivir (Dharan et al., 2 ...
... Hauge et al., 2009; Meijer et al., 2009). These resistant viruses spread globally and replaced wild-type H1N1 during 2008 (Centers for Disease Control and Prevention (CDC), 2009; Dharan et al., 2009; Hurt et al., 2009; Moscona, 2009), largely independently of the use of oseltamivir (Dharan et al., 2 ...
Leading strand
... • Mismatched nucleotides that are missed by DNA polymerase or mutations that occur after DNA synthesis is completed can often be repaired ...
... • Mismatched nucleotides that are missed by DNA polymerase or mutations that occur after DNA synthesis is completed can often be repaired ...
Herpes simplex virus latency-associated transcript gene function
... Sawtell, 1997; Sawtell and Thompson, 1992) and rabbit (Perng et al, 2000b) latency models. A small deletion in the LAT promoter alone can cause a reduction in the number of latently infected cells, indicating that the transcription of the LATs is crucial for establishing latency (Sawtell and Thompso ...
... Sawtell, 1997; Sawtell and Thompson, 1992) and rabbit (Perng et al, 2000b) latency models. A small deletion in the LAT promoter alone can cause a reduction in the number of latently infected cells, indicating that the transcription of the LATs is crucial for establishing latency (Sawtell and Thompso ...
Detecting natural selection in RNA virus populations using
... phylogenies were simulated under the range of u and t values described immediately above, with the addition of a scaled exponential growth rate of r = 200. As before, a codon-based Markov substitution model (Goldman and Yang, 1994) was used to simulate neutrally evolving sequences along the coalesce ...
... phylogenies were simulated under the range of u and t values described immediately above, with the addition of a scaled exponential growth rate of r = 200. As before, a codon-based Markov substitution model (Goldman and Yang, 1994) was used to simulate neutrally evolving sequences along the coalesce ...
Influenza: The Virus
... How to Take an NP swab Collection of a nasopharyngeal swab (NP) 1. Assemble all supplies such as gloves, mask, pen, appropriate collection kit containing ProvLab requisition, nasopharyngeal flock swab and transport medium 2. Check expiry date of transport medium 3. Perform hand hygiene by washing h ...
... How to Take an NP swab Collection of a nasopharyngeal swab (NP) 1. Assemble all supplies such as gloves, mask, pen, appropriate collection kit containing ProvLab requisition, nasopharyngeal flock swab and transport medium 2. Check expiry date of transport medium 3. Perform hand hygiene by washing h ...
Introduction - Cedar Crest College
... Until recently, it was believed that the replication complex moved along the strand of DNA. (See Figure Recent evidence suggests that the replication complex is stationary, and DNA threads through it. ...
... Until recently, it was believed that the replication complex moved along the strand of DNA. (See Figure Recent evidence suggests that the replication complex is stationary, and DNA threads through it. ...
DNA Polymerase I
... In the E. coli chromosome the origin of replication, called oriC, is characterized as being rich in: a) A-G base pairs b) A-C base pairs c) C-G base pairs d) C-T base pairs e) None of the above ...
... In the E. coli chromosome the origin of replication, called oriC, is characterized as being rich in: a) A-G base pairs b) A-C base pairs c) C-G base pairs d) C-T base pairs e) None of the above ...
DNA
... • To synthesise a new strand first an RNA primer is synthesized on the parent DNA using RNA primase • Next DNA polymerase III adds the nucleotides (to the 3´ end) added according to the complementary base pairing rules; adenine pairs with thymine and cytosine pairs with guanine; (names needed, lette ...
... • To synthesise a new strand first an RNA primer is synthesized on the parent DNA using RNA primase • Next DNA polymerase III adds the nucleotides (to the 3´ end) added according to the complementary base pairing rules; adenine pairs with thymine and cytosine pairs with guanine; (names needed, lette ...
Risks from GMOs due to Horizontal Gene Transfer
... et al., 2001; Tsirigos and Rigoutsos, 2005). A significant fraction of many prokaryote genomes, up to 20%, has been classified as recent HGTs according to these criteria (Garcia-Vallve et al., 2003). The advantage of detecting HGT by anomalous nucleotide composition is that it requires only a single ...
... et al., 2001; Tsirigos and Rigoutsos, 2005). A significant fraction of many prokaryote genomes, up to 20%, has been classified as recent HGTs according to these criteria (Garcia-Vallve et al., 2003). The advantage of detecting HGT by anomalous nucleotide composition is that it requires only a single ...
DNA virus
A DNA virus is a virus that has DNA as its genetic material and replicates using a DNA-dependent DNA polymerase. The nucleic acid is usually double-stranded DNA (dsDNA) but may also be single-stranded DNA (ssDNA). DNA viruses belong to either Group I or Group II of the Baltimore classification system for viruses. Single-stranded DNA is usually expanded to double-stranded in infected cells. Although Group VII viruses such as hepatitis B contain a DNA genome, they are not considered DNA viruses according to the Baltimore classification, but rather reverse transcribing viruses because they replicate through an RNA intermediate. Notable diseases like smallpox, herpes, and chickenpox are caused by such DNA viruses.