The origin of the 1918 pandemic influenza virus: a
... ability to bind to different forms of sialic acids and avian HAs bind poorly to the sialic acid receptors prevalent in the human respiratory tract. These different receptor affinities act as a barrier to cross-species infection. Before a virus with an avian HA can replicate and spread efficiently in ...
... ability to bind to different forms of sialic acids and avian HAs bind poorly to the sialic acid receptors prevalent in the human respiratory tract. These different receptor affinities act as a barrier to cross-species infection. Before a virus with an avian HA can replicate and spread efficiently in ...
Four-color DNA sequencing by synthesis using cleavable
... 9), sequence-specific detection of single-stranded DNA using engineered nanopores (10), and sequencing by ligation (11). More recently, DNA sequencing by synthesis (SBS) approaches such as pyrosequencing (12), sequencing of single DNA molecules (13), and polymerase colonies (14) have been widely exp ...
... 9), sequence-specific detection of single-stranded DNA using engineered nanopores (10), and sequencing by ligation (11). More recently, DNA sequencing by synthesis (SBS) approaches such as pyrosequencing (12), sequencing of single DNA molecules (13), and polymerase colonies (14) have been widely exp ...
Denaturation transition of stretched DNA
... Under physiological conditions in vitro, the thermodynamically stable configuration of DNA is the Watson–Crick double helix (however, in vivo DNA is supercoiled and constrained by proteins such as nuclear-associated proteins in prokaryotes and histones in eukaryotes). In this configuration, the nucl ...
... Under physiological conditions in vitro, the thermodynamically stable configuration of DNA is the Watson–Crick double helix (however, in vivo DNA is supercoiled and constrained by proteins such as nuclear-associated proteins in prokaryotes and histones in eukaryotes). In this configuration, the nucl ...
Assessing the Homogeneity of Plasmid DNA: An Important
... with baseline resolution. The order of migration is governed by the topology of plasmid structures. Supercoiled ccc molecules (monomers and dimers) have the most compact structure with the highest electrophoretic mobility—appearing earlier than linearized (monomers and dimers) forms that are followe ...
... with baseline resolution. The order of migration is governed by the topology of plasmid structures. Supercoiled ccc molecules (monomers and dimers) have the most compact structure with the highest electrophoretic mobility—appearing earlier than linearized (monomers and dimers) forms that are followe ...
Geometry
... Here, I show you a movie of how, actually, viruses, in this case, again, phages, are infected their hosts. This is to demonstrate that viruses actually cannot replicate on their own. They need to invade a host. They basically need to hijack the host machinery in order to produce more viruses. So, wh ...
... Here, I show you a movie of how, actually, viruses, in this case, again, phages, are infected their hosts. This is to demonstrate that viruses actually cannot replicate on their own. They need to invade a host. They basically need to hijack the host machinery in order to produce more viruses. So, wh ...
BioinformaIcs Journal Club
... include at least eight par)ally reproduc)vely isolated host races and three cryp)c species, forming a gradient of specializaBon and differenBaBon potenBally through ecological speciaBon. This complex of biotypes st ...
... include at least eight par)ally reproduc)vely isolated host races and three cryp)c species, forming a gradient of specializaBon and differenBaBon potenBally through ecological speciaBon. This complex of biotypes st ...
GM plant viruses - University of Glasgow
... Principal investigator As the principal investigator for this GM project you have a legal responsibility to ensure that all those involved or working on the project have an appropriate level of training and expertise to enable safe working. This includes ensuring that they read and understand this r ...
... Principal investigator As the principal investigator for this GM project you have a legal responsibility to ensure that all those involved or working on the project have an appropriate level of training and expertise to enable safe working. This includes ensuring that they read and understand this r ...
DNA Metallization Processes and Nanoelectronics
... in Fig. 2. Note that the conductivity of the obtained nanowires has not been measured in all reports. Generally speaking, these metallization processes of the DNA strands have been performed either (i) in solution and then the metallized DNA molecule is deposited on the substrate for the characteriz ...
... in Fig. 2. Note that the conductivity of the obtained nanowires has not been measured in all reports. Generally speaking, these metallization processes of the DNA strands have been performed either (i) in solution and then the metallized DNA molecule is deposited on the substrate for the characteriz ...
Virus induced gene silencing, a post transcriptional gene silencing
... Gene silencing at post transcriptional level, post transcriptional gene silencing (PTGS), is an RNAmediated systemic silencing mechanism which was described as quelling in fungi [1] and RNA interference in animals [2]. To specifically silence or knock down the expression of targeted gene in plants s ...
... Gene silencing at post transcriptional level, post transcriptional gene silencing (PTGS), is an RNAmediated systemic silencing mechanism which was described as quelling in fungi [1] and RNA interference in animals [2]. To specifically silence or knock down the expression of targeted gene in plants s ...
Giant viruses, giant chimeras: The multiple evolutionary histories of
... some ORFs were only found in animals (e.g., the glycosyltransferases MIMI_L230 and MIMI_R699), in bacteria and fungi (e.g., the mannosyltransferase MIMI_L373), or in a number of very diverse combinations of taxa (Supplementary table 1 in Additional data file 1). Interestingly, we found that only a v ...
... some ORFs were only found in animals (e.g., the glycosyltransferases MIMI_L230 and MIMI_R699), in bacteria and fungi (e.g., the mannosyltransferase MIMI_L373), or in a number of very diverse combinations of taxa (Supplementary table 1 in Additional data file 1). Interestingly, we found that only a v ...
Norovirus Infectious Agent Information Sheet
... Noroviruses are considered the most common cause of outbreaks of non-bacterial gastroenteritis worldwide, are the leading cause of foodborne illness in the United States (58%), and account for 26% of hospitalizations and 10% of deaths associated with food consumption. Salad ingredients, fruit, and o ...
... Noroviruses are considered the most common cause of outbreaks of non-bacterial gastroenteritis worldwide, are the leading cause of foodborne illness in the United States (58%), and account for 26% of hospitalizations and 10% of deaths associated with food consumption. Salad ingredients, fruit, and o ...
Section 1-2 Teacher Notes
... If Hershey and Chase could determine which part of the virus entered an infected cell, they would learn whether genes were made of protein or DNA. They grew viruses in cultures containing radioactive isotopes of phosphorus-32 (32P) and ...
... If Hershey and Chase could determine which part of the virus entered an infected cell, they would learn whether genes were made of protein or DNA. They grew viruses in cultures containing radioactive isotopes of phosphorus-32 (32P) and ...
The replication of DNA
... strand). DNA is copied in short segments called Okazaki fragments moving in the opposite direction to the replication fork. The lagging strand requires the repetition of primer synthesis, elongation, primer removal with gap filling and joining of Okazaki fragments. ...
... strand). DNA is copied in short segments called Okazaki fragments moving in the opposite direction to the replication fork. The lagging strand requires the repetition of primer synthesis, elongation, primer removal with gap filling and joining of Okazaki fragments. ...
Physical and Topological Properties of Circular DNA
... is arranged in one long circular unit. Hershey, Burgi, and Ingraham (5) found that the linear DNA from coliphage lambda had sticky ends which could be induced to form circular DNA. In the same year Dulbecco and Vogt (6) and Weil and Vinograd (7) showed that the DNA from the tumor virus, polyoma, was ...
... is arranged in one long circular unit. Hershey, Burgi, and Ingraham (5) found that the linear DNA from coliphage lambda had sticky ends which could be induced to form circular DNA. In the same year Dulbecco and Vogt (6) and Weil and Vinograd (7) showed that the DNA from the tumor virus, polyoma, was ...
Recombinational Circularization of Salmonella Phage
... DNA in P22-infected cells destined for lysogeny. In the following experiment, we repeated their procedure and obtained similar results. A culture of strain 18 cells growing at 37” was infected with 32P-labeled c+ phage; the multiplicity of infection, 10 per cell, was sufficient to cause the lysogeni ...
... DNA in P22-infected cells destined for lysogeny. In the following experiment, we repeated their procedure and obtained similar results. A culture of strain 18 cells growing at 37” was infected with 32P-labeled c+ phage; the multiplicity of infection, 10 per cell, was sufficient to cause the lysogeni ...
Document
... phenotypes being observed in rep strains are related to a general DNA replication problem, rather than due to some uncharacterized rep weirdness. There is more linear DNA in the absence of recBCD (recall that recBCD eats linear DNA) Observe: deletion of ruvC suppresses the linear DNA phenotype, just ...
... phenotypes being observed in rep strains are related to a general DNA replication problem, rather than due to some uncharacterized rep weirdness. There is more linear DNA in the absence of recBCD (recall that recBCD eats linear DNA) Observe: deletion of ruvC suppresses the linear DNA phenotype, just ...
Geminivirus Replication Origins Have a Modular
... TGMV A (Figure 1, cf. lanes 2 and 3), while BGMV B only replicated in the presence of BGMV A (cf. lanes 6 and 7). These results established that BGMV is able to replicate efficiently in tobacco cells and that, similar to TGMV A (Rogers et al., 1986; Hayes and Buck, 1989), BGMV A provides all of the ...
... TGMV A (Figure 1, cf. lanes 2 and 3), while BGMV B only replicated in the presence of BGMV A (cf. lanes 6 and 7). These results established that BGMV is able to replicate efficiently in tobacco cells and that, similar to TGMV A (Rogers et al., 1986; Hayes and Buck, 1989), BGMV A provides all of the ...
Oncogenes
... • Some oncogenes may be specific for particular cell types and so may not be detected with mouse flbroblasts; • Large genes may be missed because they are less likely to be transfected intact; • The NIH-3T3 cells are not 'normal' cells since they are a permanent cell line and genes involved in early ...
... • Some oncogenes may be specific for particular cell types and so may not be detected with mouse flbroblasts; • Large genes may be missed because they are less likely to be transfected intact; • The NIH-3T3 cells are not 'normal' cells since they are a permanent cell line and genes involved in early ...
CDC-and-friends-going-for-the-Polio
... but exhibits selectivities which indicate that some added circumstance enters into the determination of whether clinical or subclinical disease results upon exposure to the virus.”4 This statement was prophetic and unfortunately largely ignored. Some of the “added circumstances” that are known by po ...
... but exhibits selectivities which indicate that some added circumstance enters into the determination of whether clinical or subclinical disease results upon exposure to the virus.”4 This statement was prophetic and unfortunately largely ignored. Some of the “added circumstances” that are known by po ...
Bio II Ch 16 Molecular Basis of Inheritance
... cells. The transforming agent was found to be DNA. • Finally in 1944, Oswald Avery, Maclyn McCarty and Colin MacLeod purified various chemicals from pathogenic bacteria and showed that DNA was the transforming agent. • Still, many biologists were skeptical. In part, this reflected a belief that the ...
... cells. The transforming agent was found to be DNA. • Finally in 1944, Oswald Avery, Maclyn McCarty and Colin MacLeod purified various chemicals from pathogenic bacteria and showed that DNA was the transforming agent. • Still, many biologists were skeptical. In part, this reflected a belief that the ...
Replicon RNA Viral Vectors as Vaccines
... Vaccine development against infectious diseases has classically been based on live attenuated or inactivated infectious agents [1]. Recently, the approach of vaccination with recombinantly expressed antigens and immunogens from viral and non-viral delivery systems has been introduced to the repertoi ...
... Vaccine development against infectious diseases has classically been based on live attenuated or inactivated infectious agents [1]. Recently, the approach of vaccination with recombinantly expressed antigens and immunogens from viral and non-viral delivery systems has been introduced to the repertoi ...
Molecular Inheritance
... 2. Correct In the Hershey and Chase experiment that helped confirm that DNA, not protein, was the hereditary material, the key finding was that _____. (p. 289) Your answer: radioactively labeled phosphorus remained in the infected bacteria but not in the bacteriophage bodies Correct. When the bacter ...
... 2. Correct In the Hershey and Chase experiment that helped confirm that DNA, not protein, was the hereditary material, the key finding was that _____. (p. 289) Your answer: radioactively labeled phosphorus remained in the infected bacteria but not in the bacteriophage bodies Correct. When the bacter ...
IV. Enzymology of DNA Replication
... replication, the parent double-stranded DNA would remain intact while the daughter double-stranded DNA would be entirely newly synthesized 2. Semiconservative replication (click for diagram) a) This model predicts that both daughter DNA molecules would contain one strand each from the parent DNA and ...
... replication, the parent double-stranded DNA would remain intact while the daughter double-stranded DNA would be entirely newly synthesized 2. Semiconservative replication (click for diagram) a) This model predicts that both daughter DNA molecules would contain one strand each from the parent DNA and ...
Exporter la page en pdf
... consist of megabase-scale domains of coordinated origin firing separated by large originless transition regions. Here, we report a quantitative genome-wide analysis of DNA replication kinetics in several human cell types that contradicts this view. DNA combing in HeLa cells sorted into four temporal ...
... consist of megabase-scale domains of coordinated origin firing separated by large originless transition regions. Here, we report a quantitative genome-wide analysis of DNA replication kinetics in several human cell types that contradicts this view. DNA combing in HeLa cells sorted into four temporal ...
DNA virus
A DNA virus is a virus that has DNA as its genetic material and replicates using a DNA-dependent DNA polymerase. The nucleic acid is usually double-stranded DNA (dsDNA) but may also be single-stranded DNA (ssDNA). DNA viruses belong to either Group I or Group II of the Baltimore classification system for viruses. Single-stranded DNA is usually expanded to double-stranded in infected cells. Although Group VII viruses such as hepatitis B contain a DNA genome, they are not considered DNA viruses according to the Baltimore classification, but rather reverse transcribing viruses because they replicate through an RNA intermediate. Notable diseases like smallpox, herpes, and chickenpox are caused by such DNA viruses.