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Principle and hypothesis of genomics
Principle and hypothesis of genomics

... DNA methylation and Histone demethylation ...
Body Systems
Body Systems

... 64. Meiosis begins with one diploid cell, and ends with haploid cells. ...
No Slide Title
No Slide Title

... even between relatively close species such as Escherichia coli and Haemophilus influenzae (Koonin et al. 1996; Tatusov et al. 1996) • This breakdown in “synteny” (gene order) in prokaryotes is thought to be caused by horizontal gene transfer and also inversions around the origin of replication ...
Lecture 6 Gene expression: microarray and deep sequencing
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Perspectives Provided by Leopard and Other Cat
Perspectives Provided by Leopard and Other Cat

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Sunday, Oct - Okemos Public Schools
Sunday, Oct - Okemos Public Schools

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Introduction to Genetics and Genomics
Introduction to Genetics and Genomics

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How We Became Human: What Makes Us Different
How We Became Human: What Makes Us Different

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Chapter 11: Gene Technology
Chapter 11: Gene Technology

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Genetics and Biotechnology

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introduction - Genomics
introduction - Genomics

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GENETICS The Future of Medicine
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Genes - University of Arizona | Ecology and Evolutionary Biology
Genes - University of Arizona | Ecology and Evolutionary Biology

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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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