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class notes
class notes

... Gene research • In 1987 Nature Magazine article stated it found the gene for manic depression. It located the gene responsible as chromosome 11. Later reports refuted this discovery, and laid claim to other chromosomes responsible ie: 6, 13, 15, and 18. • Barabasi says this is not conflicting resul ...
Producing a Recombinant Plasmid, pARA-R
Producing a Recombinant Plasmid, pARA-R

... Producing a Recombinant Plasmid, pARA-R during Lab 2 will be ligated, or bonded together, using DNA ligase, making new recombinant plasmids. These newly formed plasmids will represent recombinant DNA molecules because the four restriction fragments have been recombined in different ways to produce n ...
北京大学生命科学学院
北京大学生命科学学院

... Proper DNA damage response helps cells protect genome integrity. Deregulation of this cellular process results in chromosome instability, and eventually causes cancer. Many tumor suppressors participate in DNA damage response. One typical example is BRCA1 (Breast Cancer Susceptibility Gene 1). Mutat ...
Tasks Monday January 21st 2006
Tasks Monday January 21st 2006

... other 'kingdoms'. You will collect information for these homologs (e.g. protein size, protein domains present). Using this information, you will try to find out the possible evolution for this gene and how it did arise in various organisms. Find the amino acid sequence of the E. coli photolyase pro ...
Personalized medicine - Pitt Department of Biomedical Informatics
Personalized medicine - Pitt Department of Biomedical Informatics

... – DNA sequences – Now whole genomes • Can even find single base changes, duplication, insertions, deletions • Uses advanced algorithms, programs and computational resources ...
Cystic Fibrosis - Bellarmine University
Cystic Fibrosis - Bellarmine University

... “Somatic cell hybrids are culture lines that contain the entire complement of the mouse genome and a few human chromosomes. These culture lines are developed by mixing human and rodent cells in the presence of the Sendai virus. The virus facilitates the fusing of the two cell types to form a hybrid ...
Saturday Study Session 2 Theme of the day: Information Transfer
Saturday Study Session 2 Theme of the day: Information Transfer

goals - s3.amazonaws.com
goals - s3.amazonaws.com

... • This sequence is referred to as an Alu sequence after a restriction enzyme site that is located within this 300 base pair length of DNA. ...
Bio 262- Genetics Study Guide
Bio 262- Genetics Study Guide

... Recessive: Moving back and out of view. In genetics, a recessive gene is a gene that does not express its instructions when paired with a dominant gene. Recombination: The process by which progeny derive a combination of genes different from that of either parent. In higher organisms, this can occur ...
How do organisms grow and heal themselves? What instructions do
How do organisms grow and heal themselves? What instructions do

PowerPoint Presentation Materials to accompany
PowerPoint Presentation Materials to accompany

... It is also possible for any other DNA fragment to be inserted into the plasmid And it is possible for the plasmid to circularize without an insert This is called a recircularized vector ...
The Body in Motion
The Body in Motion

... the amplified product is linked to a fluorescent reporter molecule, the fluorescence is measured at each cycle. This allows the amplification to be monitored to optimize the efficiency of amplification. ...
Rapid Communication: Mapping of the Titin (TTN) Gene to Pig
Rapid Communication: Mapping of the Titin (TTN) Gene to Pig

What you absolutely need to know for the Regents Exam
What you absolutely need to know for the Regents Exam

... These are the vocabulary words that you need to know for the genetics unit. You need to study them and quiz yourself using the castlelearning flashcard assignment that is posted. ...
DNA Marker - Faperta UGM
DNA Marker - Faperta UGM

... The determination of the position and relative distances of gene on chromosome by means of their linkage ...
Slide 1
Slide 1

... yourtheir partner’s one from their dad. We will look at size genes today. Turn over the cards to see which gene characteristics (allelles) your lambfrom will carry Each remove the two size gene cards the pack and place them Record your lamb’s gene characteristics on your sheet coloured side up on th ...
Genomics - FSU Biology - Florida State University
Genomics - FSU Biology - Florida State University

... focus in current genomics research. Easy— forward translation to peptides. Hard again — genome scale comparisons and analyses. ...
VGEC: Student Notes RESTRICTION ENZYME MAPPING OF THE λ
VGEC: Student Notes RESTRICTION ENZYME MAPPING OF THE λ

... gel is ideal for the determination of the sizes of the very largest DNA fragments. 3. You MUST deal with each gel separately when determining the sizes of the DNA fragments and plot separate standard curves for each. Measure the distance migrated by each marker DNA fragment. For each marker fragment ...
Study Guide
Study Guide

... are locations where DNA replication is really taking place? Couldn't they represent areas where DNA polymerase molecules congregate when they're not doing anything?" In particular, it lays out the evidence in favor of point (i) -- that "the presence of foci was dependent on continued DNA synthesis." ...
File - Ms. Wilson`s Biology Class
File - Ms. Wilson`s Biology Class

... http://learn.genetics.utah.edu/content/basics/builddna/ Simply build a DNA molecule with the interactive animation. Stop when it says how long it takes you to make a DNA molecule of a human being at the rate you are progressing. Read the text below and answer the following questions: 1. In order to ...
Chromosome structure & Gene Expression
Chromosome structure & Gene Expression

... repetitive, noncoding DNA sequences known as satellite DNA ...
Mitosis
Mitosis

... 1. The structure labeled X in Figure 12-1 is a(an) nucleotide (monomer). Monomers connect to form nucleic acid which is a polymer. 2. What does DNA stand for? Deoxyribonucleic acid 3. In DNA molecule nitrogen bases (C & G and A& T) are held together by hydrogen bonds. 4. The Watson and Crick model o ...
What Would You Do? - Honors 210G (Section 01): Ebola
What Would You Do? - Honors 210G (Section 01): Ebola

... scientist using a biobank sample chances upon a disease mutation and wants to get back to the donor, where does she turn? DNA and tissue deposited in such banks are usually stripped of identifying information, and the researcher who first collected them may have retired, or moved, or died. That’s one ...
Supplement 2
Supplement 2

... SDS-PAGE analysis showed that the plasmids expressed corresponding sizes of recombinant proteins (SFig. 2a). SFig. 2b illustrates the amino acid sequences of the 4 recombinant fragments deduced from DNA sequencing after cloning into the vectors. 3. Discussion and Conclusion By successfully cloning a ...
Chapter 8
Chapter 8

... A single strand of the F plasmid is transferred to the recipient cell; its complement is synthesized as it enters that cell. The strand transferred by the donor is replaced, using the remaining strand as a template for DNA synthesis. ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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