Powerpoint - Wishart Research Group
... Mix the City DNA with the Path DNA and let them randomly anneal (ligate with enzyme) After annealing/ligation they will form (7-2)! different long (150 bp) DNA molecules Select DNA molecules with the right start and ends (select by PCR) and length (gel) Sequence the DNA to determine the best pathway ...
... Mix the City DNA with the Path DNA and let them randomly anneal (ligate with enzyme) After annealing/ligation they will form (7-2)! different long (150 bp) DNA molecules Select DNA molecules with the right start and ends (select by PCR) and length (gel) Sequence the DNA to determine the best pathway ...
Bacterial Conjugation
... • F(+) bacteria creates a duplicates its plasmid (called a “daughter F factor”) • The new plasmid is transferred to the F(-) bacteria through the sex pilus • The F(-) bacteria now becomes F(+) ...
... • F(+) bacteria creates a duplicates its plasmid (called a “daughter F factor”) • The new plasmid is transferred to the F(-) bacteria through the sex pilus • The F(-) bacteria now becomes F(+) ...
Chromosomal Mapping of Murine c-fes and c
... and v-abl in Drosophila melanogaster DNA further suggests that these oncogenes may have evolved from a common ancestral gene (14). It is of interest, therefore, to determine the relative locations of the cellular oncogenes in the mammalian genome and to find out whether members of the src family of ...
... and v-abl in Drosophila melanogaster DNA further suggests that these oncogenes may have evolved from a common ancestral gene (14). It is of interest, therefore, to determine the relative locations of the cellular oncogenes in the mammalian genome and to find out whether members of the src family of ...
3D structures of RNA
... UTRs: un-translated regions (important for translational control) Exons will be spliced together by removal of the Introns Poly-adenylation site important for transcription termination (but also: mRNA stability, export mRNA from nucleus etc.) ...
... UTRs: un-translated regions (important for translational control) Exons will be spliced together by removal of the Introns Poly-adenylation site important for transcription termination (but also: mRNA stability, export mRNA from nucleus etc.) ...
Long Noncoding RNAs May Alter Chromosome`s 3D
... Our 21,000 protein-coding genes aren’t the still do not know how this spreading occurs only readable units in our genome. At last or how XIST recognizes which parts of the count, another 13,000 “genes” specify mys- X to inactivate. terious molecules called long noncoding When Engreitz arrived in Gut ...
... Our 21,000 protein-coding genes aren’t the still do not know how this spreading occurs only readable units in our genome. At last or how XIST recognizes which parts of the count, another 13,000 “genes” specify mys- X to inactivate. terious molecules called long noncoding When Engreitz arrived in Gut ...
E. coli - Sonoma Valley High School
... Samples from the restriction enzyme digests are introduced into the gel. Electric current is applied causing fragments to migrate through the gel. ...
... Samples from the restriction enzyme digests are introduced into the gel. Electric current is applied causing fragments to migrate through the gel. ...
From Atoms to Traits
... known as microsatellites that consist of sequences of two, three or more nucleotides repeated over and over. All these spontaneous changes within genomes add up to a lot of diversity, even within a single species, including our own. In a historic milestone, a reference sequence for the entire threeb ...
... known as microsatellites that consist of sequences of two, three or more nucleotides repeated over and over. All these spontaneous changes within genomes add up to a lot of diversity, even within a single species, including our own. In a historic milestone, a reference sequence for the entire threeb ...
Anatomy and Physiology BIO 137
... joining together of DNA molecules (from two different species) that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. ...
... joining together of DNA molecules (from two different species) that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. ...
AP Biology: Evolution
... produce RFLPs (fragments). Sample D is DNA that has not been cut with enzyme(s). DNA cut with HindIII provides a set of fragments of known size and serves as a standard for comparison. 2. Using the ideal gel shown in Figure 5, measure the distance (in cm) that each fragment migrated from the origin ...
... produce RFLPs (fragments). Sample D is DNA that has not been cut with enzyme(s). DNA cut with HindIII provides a set of fragments of known size and serves as a standard for comparison. 2. Using the ideal gel shown in Figure 5, measure the distance (in cm) that each fragment migrated from the origin ...
Answers to Problem Set 3A
... Note that the enzymes (numbers) are placed in locations they are likely to be functioning with respect to the way the DNA molecules are presented (ie, the topology of the DNA). This is a simplified view. For example, you could have placed DNA polymerase III (the circled number 6) at either of the t ...
... Note that the enzymes (numbers) are placed in locations they are likely to be functioning with respect to the way the DNA molecules are presented (ie, the topology of the DNA). This is a simplified view. For example, you could have placed DNA polymerase III (the circled number 6) at either of the t ...
Elucidating the essentiality of essential genes in E. coli K-12
... coli K-12 by Wanner’s method [1] and simultaneously 303 genes have been predicted as essential genes in LB medium [2]. Elucidating the essentiality of these essential genes is a key to understand the system level organization of living cells. Here we present our analysis to address this issue from t ...
... coli K-12 by Wanner’s method [1] and simultaneously 303 genes have been predicted as essential genes in LB medium [2]. Elucidating the essentiality of these essential genes is a key to understand the system level organization of living cells. Here we present our analysis to address this issue from t ...
pGLO Transformation Lab - Tamalpais Union High School District
... gene for resistance to the antibiotic “ampicillin”. The gene for the Green Fluorescent Protein can only be switched on in transformed cells by adding the sugar “arabinose” to the cells nutrient medium (food). Transformed cells will appear white on plates not containing the sugar arabinose, and fluor ...
... gene for resistance to the antibiotic “ampicillin”. The gene for the Green Fluorescent Protein can only be switched on in transformed cells by adding the sugar “arabinose” to the cells nutrient medium (food). Transformed cells will appear white on plates not containing the sugar arabinose, and fluor ...
level one science: biology
... explaining the link between a change in the genetic code and a change in phenotype. I can explain how a mutation can lead to a new allele by linking genotype and phenotype. I can discuss why mutations are only passed on if they occur in gametes. ...
... explaining the link between a change in the genetic code and a change in phenotype. I can explain how a mutation can lead to a new allele by linking genotype and phenotype. I can discuss why mutations are only passed on if they occur in gametes. ...
Document
... Fill-in the blanks of questions #16-21 with the best term or number (2 pts. for each blank): 16. The haploid chromosome number in humans is n=23. In the space provided, give the number of indicated structures that should be present in a single cell at the indicated time during oogenesis: a. Chromati ...
... Fill-in the blanks of questions #16-21 with the best term or number (2 pts. for each blank): 16. The haploid chromosome number in humans is n=23. In the space provided, give the number of indicated structures that should be present in a single cell at the indicated time during oogenesis: a. Chromati ...
- mrsolson.com
... c. intended to destroy foreign DNA that enters the cell d. used to attach pieces of DNA together 49. Which of the following is mismatched? a. bioinformatics—the study of a genome using computer analysis b. polymerase chain reaction—process that separates DNA fragments according to size c. genomics—t ...
... c. intended to destroy foreign DNA that enters the cell d. used to attach pieces of DNA together 49. Which of the following is mismatched? a. bioinformatics—the study of a genome using computer analysis b. polymerase chain reaction—process that separates DNA fragments according to size c. genomics—t ...
recombinant dna technology and genetic engineering
... GGATCC 3' 3' CCTAGG 5'. The ends of the cut have an overhanging piece of single-stranded DNA. These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end. In this case, both DNA preparations have complementary sticky ends and thus c ...
... GGATCC 3' 3' CCTAGG 5'. The ends of the cut have an overhanging piece of single-stranded DNA. These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end. In this case, both DNA preparations have complementary sticky ends and thus c ...
Mutations (1 of 2)
... change in an organism’s DNA can cause changes in all aspects of its life. Mutations are random. Mutations can be beneficial, neutral, or harmful for the organism, but mutations do not “try” to supply what the organism “needs.” In this respect, mutations are random— whether a particular mutation happ ...
... change in an organism’s DNA can cause changes in all aspects of its life. Mutations are random. Mutations can be beneficial, neutral, or harmful for the organism, but mutations do not “try” to supply what the organism “needs.” In this respect, mutations are random— whether a particular mutation happ ...
What are Math and Computer Science doing in Biology?
... High-throughput biology generating massive amounts of data; sometimes too large even to store. ...
... High-throughput biology generating massive amounts of data; sometimes too large even to store. ...
Characteristics of Living Things (Essay
... chromosomes? What are alleles? In comparing a pair of homologous chromosomes - chromosome #1 in Humans for example - is it likely for this pair of chromosomes to contain identical alleles at each and every gene loci along the entire chromosome? Please explain your reasoning carefully! Explain the pr ...
... chromosomes? What are alleles? In comparing a pair of homologous chromosomes - chromosome #1 in Humans for example - is it likely for this pair of chromosomes to contain identical alleles at each and every gene loci along the entire chromosome? Please explain your reasoning carefully! Explain the pr ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.