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... B) cannot be produced by mitosis. C) function as gametes or spores in sexually reproducing organisms. D) have one member of each pair of homologous chromosomes. E) Both choices A and C are correct. 20) Which of the following statements is FALSE? A) Meiosis separates homologous chromosomes in a diplo ...
Molecular Biology Unit Notes
Molecular Biology Unit Notes

... A-site holds tRNA to be added to the chain with a amino acid E-site is where the discharged tRNAs leave the ribosome 2. tRNA (transfer) reads Codons (made up of three consecutive nucleotides) a. tRNA transfers amio acids from the cytoplasm to the growing amio-acid chain b. tRNA is made up of one sid ...
Day1-UVM-2ndvisit-Pombe
Day1-UVM-2ndvisit-Pombe

... than chlorine, chlorine dioxide, and potassium permanganate. And through catalysis, H2O2 can be converted into hydroxyl radicals (.OH) with reactivity second only to fluorine. • Grow the yeast and treat the control group with buffer (HBSS) and the treated group with buffer containing 0.5 mM H2O2 • I ...
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Biotechnology

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Attachment A - Recombinant DNA and Viral

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Molecular and Genomics-Based Diagnostics for Medical Microbiology
Molecular and Genomics-Based Diagnostics for Medical Microbiology

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Antibiotic resistance genes are carried on plasmids
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GenomeWeb Stanford Team Shows How Long Reads Can

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... Nondisjunction in meiosis I results in all the gametes having abnormal numbers of chromosomes. Nondisjunction in meiosis II results in two normal gametes and two abnormal gametes. (Note that for simplicity only two pairs of homologous chromosomes are shown rather than all 23 pairs.) ...
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... A trait is said to be monogenic if the phenotypic variation between two individuals or two strains of organisms can be explained by differences in a single gene. In this example, a single malfunctioning gene can result in an unpigmented animal; for example, if no pigment cells are made (because a ge ...
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... Vectors are available for fusion of your ORF to fluorescent proteins or localization signals. Vectors are also available with IRES or CHYSEL sequences for translational coupling of your ORF and a fluorescent protein to provide expression monitoring. Some vectors contain mammalian selectable markers ...
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... 11. Describe the structure of DNA. 12. Briefly describe the work of the scientists pg 196. 13. Thoroughly describe the process of DNA replication 14. Describe all key terms page 201. Chapter 10 Know EVERYTHING in this chapter! Review activity- Do this now! 15. Write in paragraph form: How do protein ...
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... 138. By using the techniques of genetic engineering, scientists are able to modify genetic material so that a particular gene of interest from one cell can be incorporated into a different cell. 1. Describe a procedure by which this can be done. 2. Explain the purpose of each step of your procedure. ...
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Bacterial Conjugation

... • F(+) bacteria creates a duplicates its plasmid (called a “daughter F factor”) • The new plasmid is transferred to the F(-) bacteria through the sex pilus • The F(-) bacteria now becomes F(+) ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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