Recovery of DNA for Forensic Analysis from Lip Cosmetics*
... taken even if it does not appear that the individual is wearing any lipstick, or if the person being sampled is male. The presence of fluorescent artifacts in a DNA profile obtained from a buccal swab may be indicative of contamination by lip cosmetics and alternative DNA extraction methods may be r ...
... taken even if it does not appear that the individual is wearing any lipstick, or if the person being sampled is male. The presence of fluorescent artifacts in a DNA profile obtained from a buccal swab may be indicative of contamination by lip cosmetics and alternative DNA extraction methods may be r ...
Degenerate PCR - Yale School of Medicine
... doesn't fit quite correctly in double stranded DNA, so there may be an energetic penalty to pay when the helix bulges out at this purine:purine pairing). Recently, most people have been using inosine in their primers at positions where any of the four bases might be required. Each use of inosine thu ...
... doesn't fit quite correctly in double stranded DNA, so there may be an energetic penalty to pay when the helix bulges out at this purine:purine pairing). Recently, most people have been using inosine in their primers at positions where any of the four bases might be required. Each use of inosine thu ...
Publishing life science data as linked open data: the case study of
... is important for therapeutic uses. For example, based on such knowledge, biologists can shut off genes by delivering artificial miRNA molecules into cells. The first miRNA molecules were identified in 1993. Since then, there has been a dramatic increase in the number of miRNAs discovered and registe ...
... is important for therapeutic uses. For example, based on such knowledge, biologists can shut off genes by delivering artificial miRNA molecules into cells. The first miRNA molecules were identified in 1993. Since then, there has been a dramatic increase in the number of miRNAs discovered and registe ...
Evolutionary Genomics of Fast Evolving Tunicates
... Another peculiarity that contributed in a very significant way to genome compaction in O. dioica is that the majority of genes contain very small introns. In fact, most introns are very short, being on average less than 50 nt in length, and only 2.4% of them are larger than 1 kb (Denoeud et al. 2010 ...
... Another peculiarity that contributed in a very significant way to genome compaction in O. dioica is that the majority of genes contain very small introns. In fact, most introns are very short, being on average less than 50 nt in length, and only 2.4% of them are larger than 1 kb (Denoeud et al. 2010 ...
The Functions of Introns: From Junk DNA to Designed DNA
... introns are identified by specific consensus sequences [GT at the start or donor (3’) end and AG at the other end, called the acceptor (5’) site] which help to identify introns for removal. The term “consensus sequence” is employed because, although the sites may consist of more than just GT donor a ...
... introns are identified by specific consensus sequences [GT at the start or donor (3’) end and AG at the other end, called the acceptor (5’) site] which help to identify introns for removal. The term “consensus sequence” is employed because, although the sites may consist of more than just GT donor a ...
Mating-Type Genes From the Homothallic Fungus Sordaria
... that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region is shown in Figure 1. DNA sequence analysis: To ...
... that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region is shown in Figure 1. DNA sequence analysis: To ...
Bacteria in Permafrost
... Acidobacteria, Firmicutes, Bacteroidetes, Verrucomicrobia, and Cyanobacteria. All libraries contained a large proportion of RSTs (10–25%) with close affiliations to 16S rRNA gene sequences of unknown phylogenetic affiliation. This report and our studies demonstrate that the Arctic serves as an unrec ...
... Acidobacteria, Firmicutes, Bacteroidetes, Verrucomicrobia, and Cyanobacteria. All libraries contained a large proportion of RSTs (10–25%) with close affiliations to 16S rRNA gene sequences of unknown phylogenetic affiliation. This report and our studies demonstrate that the Arctic serves as an unrec ...
Alu Human Polymorphism
... • This will produce the alignment with the details about what bases were aligned and the % of identity – This will give you three alignments with the Alu element ...
... • This will produce the alignment with the details about what bases were aligned and the % of identity – This will give you three alignments with the Alu element ...
Trachemys scripta elegans Red-Eared Turtle ( Fc)
... The 115 unique VH clones accounted for a total of 61 unique CDR3s, which resulted from independent VDJ recombination events. The lengths of the CDR3s (according to the IMGT numbering system) among these clones ranged from 7 to 21 aa (Supplemental Fig. 2), with an average length of 11.4 6 2.5 aa. Alt ...
... The 115 unique VH clones accounted for a total of 61 unique CDR3s, which resulted from independent VDJ recombination events. The lengths of the CDR3s (according to the IMGT numbering system) among these clones ranged from 7 to 21 aa (Supplemental Fig. 2), with an average length of 11.4 6 2.5 aa. Alt ...
The Birth- and- Death Evolution of Multigene Families Revisited
... expansions (or contractions) of many multigene families, as well as more general features that characterize their evolution. The most important limitations are the following: (1) quite often, so-called complete genomes are not fully completed and are very fragmented. This is a very important proble ...
... expansions (or contractions) of many multigene families, as well as more general features that characterize their evolution. The most important limitations are the following: (1) quite often, so-called complete genomes are not fully completed and are very fragmented. This is a very important proble ...
Erp, an extracellular protein family specific to
... paratuberculosis (http :\\www.cbc.umn.edu\cgi-bin\ blasts\AGAC.restrict\blastn.cgi) were released, showing the erp gene to be present in both species, thereby confirming and extending our data. It is thus very likely that erp is common to all members of the genus Mycobacterium. Conservation of the e ...
... paratuberculosis (http :\\www.cbc.umn.edu\cgi-bin\ blasts\AGAC.restrict\blastn.cgi) were released, showing the erp gene to be present in both species, thereby confirming and extending our data. It is thus very likely that erp is common to all members of the genus Mycobacterium. Conservation of the e ...
DNA Denaturing through UV-C Photon Dissipation: A
... sample, the surviving light was collected by a second lens and focused onto a similar optical fiber which fed into an Ocean Optics@ HR4000CG charge-coupled device spectrometer. The spectrometer covered the range 200 to 1200 nm with a resolution of 0.3 nm. Spectrometer integration times were of the o ...
... sample, the surviving light was collected by a second lens and focused onto a similar optical fiber which fed into an Ocean Optics@ HR4000CG charge-coupled device spectrometer. The spectrometer covered the range 200 to 1200 nm with a resolution of 0.3 nm. Spectrometer integration times were of the o ...
Susan Evans, David Green, and Angela Hatton
... inlet with luer valve and glas frit, 4.glass tubing with glass wool. 5. Nafion dryer encased in molecular sieve type 13 x, 6. 6 port sample injection valve. 7. Sample loop 8. ...
... inlet with luer valve and glas frit, 4.glass tubing with glass wool. 5. Nafion dryer encased in molecular sieve type 13 x, 6. 6 port sample injection valve. 7. Sample loop 8. ...
From Genetics to DNA
... as histones compact and organize DNA, which helps control its interactions with other proteins and thereby control which genes are transcribed. Physical and chemical properties DNA is a long polymer made from repeating units called nucleotides. The DNA chain is 22 to 26 Ångströms wide (2.2 to 2.6 n ...
... as histones compact and organize DNA, which helps control its interactions with other proteins and thereby control which genes are transcribed. Physical and chemical properties DNA is a long polymer made from repeating units called nucleotides. The DNA chain is 22 to 26 Ångströms wide (2.2 to 2.6 n ...
An assessment of the risks associated with the
... the plant cell where the natural integration properties of the T-DNA insert the entire gene ensemble into the chromosomal DNA.6,9–11 Engineered plant DNA can also be delivered into plant cells by particle bombardment (biolistic transformation), when the DNA is literally shot into plant cells on DNA- ...
... the plant cell where the natural integration properties of the T-DNA insert the entire gene ensemble into the chromosomal DNA.6,9–11 Engineered plant DNA can also be delivered into plant cells by particle bombardment (biolistic transformation), when the DNA is literally shot into plant cells on DNA- ...
Rapid identification of health care–associated infections with an
... Although bacterial culture is the clinical gold standard, it has drawbacks including long process times (up to several days), personnel cost, and the need for specialized equipment and species-specific protocols. As an alternative, nucleic acid (NA) testing has been increasingly adopted in clinical ...
... Although bacterial culture is the clinical gold standard, it has drawbacks including long process times (up to several days), personnel cost, and the need for specialized equipment and species-specific protocols. As an alternative, nucleic acid (NA) testing has been increasingly adopted in clinical ...
SAB-2010
... Based on the SAB suggestions, the progress has been made towards the goal of extending the annotation of pathway databases in Cyc and Wiki versions in an automated way. However to do that approach we have to streamline the data workflow and structure the current curated gene database as a central re ...
... Based on the SAB suggestions, the progress has been made towards the goal of extending the annotation of pathway databases in Cyc and Wiki versions in an automated way. However to do that approach we have to streamline the data workflow and structure the current curated gene database as a central re ...
Determination of obesity associated gene variants related
... and adolescents with ages ranging from 9 to 13 years old. This cohort is part of the ‘Healthy Growth Study’, a large-scale cross-sectional epidemiological study initiated in May 2007 and described previously (Moschonis et al., 2010). Swedish and Greek populations, despite having a common origin, dis ...
... and adolescents with ages ranging from 9 to 13 years old. This cohort is part of the ‘Healthy Growth Study’, a large-scale cross-sectional epidemiological study initiated in May 2007 and described previously (Moschonis et al., 2010). Swedish and Greek populations, despite having a common origin, dis ...
Scoring Guidelines - Ohio Assessment Systems
... Life is specified by genomes. Each organism has a genome that contains all of the biological information needed to build and maintain a living example of that organism. The biological information contained in a genome is encoded in its deoxyribonucleic acid (DNA) and is divided into discrete units c ...
... Life is specified by genomes. Each organism has a genome that contains all of the biological information needed to build and maintain a living example of that organism. The biological information contained in a genome is encoded in its deoxyribonucleic acid (DNA) and is divided into discrete units c ...
Sequence of the Tribolium castaneum Homeotic Complex
... cluster of Caenorhabditis elegans (C. Elegans Sequencing Consortium 1998) have been sequenced. Analysis of homeobox sequences from several arthropod classes (for a recent synthesis see Cook et al. 2001) and an onychophoran (Grenier et al. 1997) indicates that precursors of eight Hox genes, as well a ...
... cluster of Caenorhabditis elegans (C. Elegans Sequencing Consortium 1998) have been sequenced. Analysis of homeobox sequences from several arthropod classes (for a recent synthesis see Cook et al. 2001) and an onychophoran (Grenier et al. 1997) indicates that precursors of eight Hox genes, as well a ...
Optimizing Restriction Site Placement for Synthetic
... prized, as they cut the sequence unambiguously in exactly one place. Many techniques for manipulating DNA make use of unique restriction sites [6, 7]. In particular, subcloning is an important method of inserting a new sequence between two different unique restriction sites. Thus a genomic sequence ...
... prized, as they cut the sequence unambiguously in exactly one place. Many techniques for manipulating DNA make use of unique restriction sites [6, 7]. In particular, subcloning is an important method of inserting a new sequence between two different unique restriction sites. Thus a genomic sequence ...
Laboratory manual for the diagnosis of whooping cough caused by... pertussis/ Bordetella parapertussis
... Even in high-coverage countries, pertussis disease continues to cause severe illness and death among neonates and infants too young to have completed the primary vaccination series. Active primary immunization against B. pertussis infection is recommended with three doses of a vaccine consisting of ...
... Even in high-coverage countries, pertussis disease continues to cause severe illness and death among neonates and infants too young to have completed the primary vaccination series. Active primary immunization against B. pertussis infection is recommended with three doses of a vaccine consisting of ...
Use of Alternative Primers for Gender Discrimination in Human
... protocol based on the use of primers from the CODIS set, including the gender determining marker, amelogenin,9 using microfluidics rather than capillary electrophoresis for amplicon detection. Based on the low but significant probability of misidentifying samples, we considered it advantageous to ou ...
... protocol based on the use of primers from the CODIS set, including the gender determining marker, amelogenin,9 using microfluidics rather than capillary electrophoresis for amplicon detection. Based on the low but significant probability of misidentifying samples, we considered it advantageous to ou ...
Standard Genetic Nomenclature - Iowa State University Digital
... (e.g. coat colour), etc. As such, there is a good chance a trait will be named differently by different people, even within a species community. Furthermore, traits have been studied across many species, which adds additional complexity to their naming. The study of traits may also involve the study ...
... (e.g. coat colour), etc. As such, there is a good chance a trait will be named differently by different people, even within a species community. Furthermore, traits have been studied across many species, which adds additional complexity to their naming. The study of traits may also involve the study ...
Standard Genetic Nomenclature - Iowa State University Digital
... (e.g. coat colour), etc. As such, there is a good chance a trait will be named differently by different people, even within a species community. Furthermore, traits have been studied across many species, which adds additional complexity to their naming. The study of traits may also involve the study ...
... (e.g. coat colour), etc. As such, there is a good chance a trait will be named differently by different people, even within a species community. Furthermore, traits have been studied across many species, which adds additional complexity to their naming. The study of traits may also involve the study ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.