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What are genomes and how are they studied
What are genomes and how are they studied

... 3) Single nucleotide polymorphisms (SNP) identificationSites that result from point mutations in individual base pairs ...
Lecture 23: Powerpoint
Lecture 23: Powerpoint

... To understand more about the processes of inheritance and gene expression To provide better understanding & treatment of various diseases, particularly genetic disorders To generate economic benefits, including improved plants and animals for agriculture and efficient production of valuable biologic ...
handout 1
handout 1

... The traditional approach to identifying bacterial strains is based largely on growthdependent physiological and biochemical tests that have been developed since the beginning of the 20th Century, and are still widely used in clinical laboratories. We perform a number of these classic diagnostic test ...
Bioinformatics Tools
Bioinformatics Tools

... transcribed elements, including non-coding RNAs used for structural and regulatory purposes. ...
Integration within Health-care records
Integration within Health-care records

... We have demonstrated the significance of information fusion based tools for bio-geo health care informatics. • As a data warehouse for various data sets involved in bio-geo health care informatics studies. • To provide and demonstrate a set of information fusion tools for disease research. ...
IntrotoBiotechRestrictionEnzymes2011
IntrotoBiotechRestrictionEnzymes2011

... • some restriction enzymes (like EcoRI) produce cuts in the DNA that result in the formation of sticky ends on the DNA fragments that are formed. • sticky ends indicates that unpaired bases are left hanging off the cut. other restriction enzymes produce blunt ends, that is, the DNA is cut directly ...
Genomics in NBS: potential targets and benefits
Genomics in NBS: potential targets and benefits

... of medicine that involves using genomic data to better predict, diagnose, and treat disease • New technologies have driven advances in genomic medicine in last 10 years and will in the future • Genomic sequencing now cheaper and faster - $1000 genome ...
Researchers ACT on DNA Storage
Researchers ACT on DNA Storage

... Unlike many forms of information storage, DNA is extremely long-lasting and does not require constant electrical power. Plus, it's tiny—a small cup of DNA can store one hundred million hours of high-quality video. But until now, this storage method has faced too many obstacles: DNA synthesis is expe ...
AP Biology - gwbiology
AP Biology - gwbiology

... Outline the diagram below of Dideoxy Chain Termination – I know this seems difficult to follow at first but at least copy the main ideas before we go over it in class. ...
F4-6 Gene Regulation and Mutation Ch12,13
F4-6 Gene Regulation and Mutation Ch12,13

... d. Clean trash/toxic chemicals C. Human Genome 1. Genome – complete genetic info in a cell 2. Human genome project – completed 2003 a. determine sequence of 3 billion nucleotides b. ID 20-25,000 human genes 3. Accomplished a. Used restriction enzymes to cut all genes from 46 chromosomes 4. Findings ...
- University of East Anglia
- University of East Anglia

... metagenomics (Nat Rev Microbiol 2005; 3 (6): 504-10, Curr Opin Virol 2012: 2(1); 63-77). Bacteriophages are viruses that are specific to the bacteria on whom they predate and they are thought to play a crucial role in maintaining the microbial balance in every ecosystem including the human intestine ...
What is a Genome? - Auburn University
What is a Genome? - Auburn University

... molecules (sequence of base pairs in the DNA). There is other important information required for a gene to specific a trait, for example, other information is sustained in each cellular generation at the chromosomal level, and finally the genome as a whole produces interactions that further determin ...
zChap11_140901 - Online Open Genetics
zChap11_140901 - Online Open Genetics

... Interpretation of the large data sets generated by –omics research depends on a combination of computational, biological, and statistical knowledge provided by ...
The Human Genome
The Human Genome

... • Viruses are used because they can enter a cells DNA. They are modified so they can’t cause a disease. • The DNA fragment containing the replacement gene is spliced to viral DNA and the patient is infected with the modified virus particles, that carry the gene into the cells to correct genetic defe ...
BSC 219
BSC 219

... The mutation caused an increase in transcription because it resulted in a 10 sequence that now has the strongest possible sequence for recruiting sigma factor and RNA Polymerase (TATAAT). This would result in an increase in initiation and a resultant increase in transcription of the gene. ...
PPT - Larry Smarr - California Institute for Telecommunications and
PPT - Larry Smarr - California Institute for Telecommunications and

... Most human disease results from a combination of inherited genetic variations and environmental factors (such as lifestyle, social conditions, chemical exposures, and infections). Thanks to the genome-based tools now available to public health researchers, we can study how and where disease occurs i ...
Ensembl - Internet Database Lab.
Ensembl - Internet Database Lab.

... Transcript/translation Summary report ...
Population Genetics and a Study of Speciation Using Next
Population Genetics and a Study of Speciation Using Next

... Instead of sequencing only one DNA fragment at a time, methods are now available to sequence billions of DNA fragments simultaneously. This makes it feasible for individual researchers to sequence the genomes of their favorite organisms as well as to survey genomic variation within and between close ...
Supplementary Text 1 (doc 52K)
Supplementary Text 1 (doc 52K)

... parallel primers for the MMC were designed (see above), which were also used as signature sequences for the MMC. Using these sequences in another BLAST analysis we rechecked the results obtained by BLAST with the almost complete 16S rRNA gene sequences and the phylogenetic analysis. Finally, BLAST a ...
L04_Public_Resources_Luke_Durban_2015
L04_Public_Resources_Luke_Durban_2015

... http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/human/ It looks like this: ...
Restriction Mapping Restriction Fragment Length Polymorphism
Restriction Mapping Restriction Fragment Length Polymorphism

... These are 1 to 5 kb in length consisting of repeats 15 to 100 nucleotides in length and are identified by Southern analysis. 2. Microsatellite DNA ...
Supplementary data
Supplementary data

... and Porphyromonas gingivalis ATCC33277, Actinomyces odontolyticus ATCC 17982 [7,8]. Remarkably, when the genome sequence of B. dentium Bd1 was compared to a genome database representing these oral pathogenic strains mentioned above (oral pathogen pangenome), a large number (around 1390) of significa ...
New Microsoft Office PowerPoint Presentation
New Microsoft Office PowerPoint Presentation

... Expressed Sequence Tags (ESTs) • Unedited, short, single pass sequences generated from 5' or 3' end of randomly selected cDNA libraries in desired cells/tissues/organ. • Length: 200-700 bp (average 360 bp) • Can be quickly generated at low cost (“poorman’s genome”) • EST annotations have very littl ...
Library types
Library types

... claiming patent rights over the sequences they obtained first and the consortium turning its data over to the public domain • With everything cloned and sequenced, it is now possible to “clone by phone” any gene of piece of human DNA if some sequence data is acquired or if the chromosomal location o ...
5.genome-browsers
5.genome-browsers

... Now that most model organisms have had their genomes sequenced, we can get a lot more information about how the gene works, than by just doing a BLAST search against the protein databases. Even if ‘your’ favourite genome is still just in ‘scaffolds’ and not yet assembled into chromosomes, we can sti ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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