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PTC bioinformatics
PTC bioinformatics

... that sequence the restriction enzyme will cleave the gene at that locality. Non tasters do not show this sequence and so in this 221 base pair region of the DNA, the segment stays whole. If a classmate was a taster, their DNA would be cleaved leaving a 44 and 177 base pair segment, which would be ab ...
source file
source file

... http://img.jgi.doe.gov/cgi-bin/pub/main.cgi INTEGRATED MICROBIAL GENOMES (IMG) - Database managed by the U.S. Department of Energy (DOE) Joint Genome Institute (JGI) - JGI currently producing ~ 22% of the reported number of bacterial genome projects worldwide - Key mission of IMG is to provide a dat ...
Title:  P.I.’s :
Title: P.I.’s :

... This is the first study to characterize DNA methylation profiles and DNMT genes in an aquatic species of environmental relevance. Our results demonstrated that global methylation levels are not between sensitive (SC) and resistant (NBH) fish. However, we observed differences in the levels of hydrox ...
Gypsy Vanner Horse Society DNA Analysis Form
Gypsy Vanner Horse Society DNA Analysis Form

... the test will be held by the Gypsy Vanner Horse Society and the University of Kentucky. The University may use the samples in its research. The GVHS does authorize the University of Kentucky to share this DNA information with certain other registries as needed to verify parentage. Hair samples are c ...
Transcription/Translation
Transcription/Translation

... • Using Mendelian and other genetic analyses we have talked about, everything we have deduced about genes and DNA sequence has been indirect • With recombinant DNA technology we can isolate genes and DNA sequence, study them directly and store it in a convenient manner that facilitates future applic ...
Sequencing the Human Genome
Sequencing the Human Genome

... 2. “Shock” these into the DNA of e-coli bacteria, and let them replicate the BACs to any degree. 3. Take each BAC and cut it into manageable pieces, using restriction enzymes. 4. Clone (artificially replicate) these pieces, so as to have enough to work with. This is known as PCR, or polymerase chain ...
C. elegans
C. elegans

... 1. There are few, if any, reciprocal translocations between chromosomes, unlike yeasts (and mammals), but like Drosophila flies. No idea why! 2. What regions of introns can be aligned, and are hence somewhat conserved, are implicated in regulation of alternative splicing, that is, determining which ...
Basic Bioinformatics Laboratory
Basic Bioinformatics Laboratory

... 5. Choose one of the comparisons (with at least five organisms) and using the human as a base, count the number of amino acid differences for the other organisms. 6. Using this data, calculate the % of similarity of each organism to the human. 100 – (Number of differences/total amino acids X 100) = ...
Experimental Ecology
Experimental Ecology

... • Problems: cross-reactivity, can’t raise antibodies if you don’t have a pure culture and so can’t predict if any other microbe will also react; change of antigenic properties is response to environment; sometimes not very sensitive; can be very time-consuming ...
AP Biology Name Cladistics and Bioinformatics Questions 2013 The
AP Biology Name Cladistics and Bioinformatics Questions 2013 The

... An outgroup is a species of organism that acts as a reference group when determining the evolutionary relationships between three or more other species of organisms. It is hypothesized to be related to the species in question but less closely than any of the other species are to each other. Outgroup ...
Evolution Cladistics Questions 2013
Evolution Cladistics Questions 2013

... An outgroup is a species of organism that acts as a reference group when determining the evolutionary relationships between three or more other species of organisms. It is hypothesized to be related to the species in question but less closely than any of the other species are to each other. Outgroup ...
AP Biology
AP Biology

... 25. Define and explain the significance of RFLP’s – restriction length polymorphisms. ...
Tutorial_12 (2014)
Tutorial_12 (2014)

... http://genome.ucsc.edu/goldenPath/help/hgTracksHelp.html#CustomTracks ...
Wednesday, September 5
Wednesday, September 5

... genetic mapping and physical mapping stages; instead, short fragments generated by multiple restriction enzymes are sequenced and then subsequently ordered by computer programs that identify overlapping regions. ...
ANSWERS TO REVIEW QUESTIONS
ANSWERS TO REVIEW QUESTIONS

... 4. A single gene can control the rates of development of specific structures, causing enormous differences in the relative sizes of organs in two species. 5. Limited fossil or genetic evidence provides only a partial picture. For example, the sequence of the Neanderthal genome indicated that we shar ...
Overview
Overview

... •First pass alignment based upon non-masked sequences to produce contiguous sequence fragments •Alignments must account for potential polymorphisms •Repetitive sequences still need to be aligned - their treatment is however distinct from non-repetitive sequences •Resolution of conflicts in the assem ...
Affymetrix Resequencing Arrays
Affymetrix Resequencing Arrays

... Autosomal recessive disorders are a major cause of infant morbidity and mortality Significantly higher in WM than rest of country (Bundy report, 1990) Clinical phenotypes can be caused by mutations in one of several genes or different mutated genes can cause very similar clinical phenotype Genes are ...
Reproductive Technology
Reproductive Technology

... • Sequences overlapped and built up ...
Lecture8-Chap5 Sept26
Lecture8-Chap5 Sept26

... identifying coding regions by identifying fragments whose sequences are present in multiple organisms. • zoo blot – The use of Southern blotting to test the ability of a DNA probe from one species to hybridize with the DNA from the genomes of a variety of other species. • Human disease genes are ide ...
Lecture8-Chap5 Sept26
Lecture8-Chap5 Sept26

... identifying coding regions by identifying fragments whose sequences are present in multiple organisms. • zoo blot – The use of Southern blotting to test the ability of a DNA probe from one species to hybridize with the DNA from the genomes of a variety of other species. • Human disease genes are ide ...
phylogenetic tree.
phylogenetic tree.

... phenotypic similarity.  They do not show the age of the particular species. ...
T. brucei
T. brucei

... sequence in version 3.1, has brought the current total number in GeneDB (the “official” repository for LmjF annotation) to 8151. T. cruzi - AutoMAGI used to predict probable protein-coding genes. Due to the complex organization of the T. cruzi genome discussed above, a total of 25,235 genes have bee ...
Enterococcus faecalis VRE, Genomic DNA
Enterococcus faecalis VRE, Genomic DNA

... genomic tips. This control is supplied in TE Buffer and should be frozen at -20°C or below. DNA concentration and 260/280 ratios are determined using a NanoDrop ND-1000®. The extracted DNA ...
Pan-genomics: Unmasking the gene diversity hidden in the bacteria
Pan-genomics: Unmasking the gene diversity hidden in the bacteria

... spaces and lower amount of repetitive DNA when comparing with eukaryotes. So, in bacteria differences in genome size correlates directly with coding sequences, the larger of your bacterial genome, the more functions you can potentially perform with the genes coded in your genome. S. agalactiae’s var ...
Lec #6 - University of San Diego Home Pages
Lec #6 - University of San Diego Home Pages

... • Are normally caught in nets of various mesh sizes and are therefore often classified by size ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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