day2
... • Many popular tools of recDNA rely on the principle of DNA hybridization. • In large mixes of DNA molecules, complementary sequences will pair. ...
... • Many popular tools of recDNA rely on the principle of DNA hybridization. • In large mixes of DNA molecules, complementary sequences will pair. ...
microarrays part1
... Massively parallel analysis of gene expression • screen an entire genome at once • find not only individual genes that differ, but groups of genes that differ. • find relative expression level differences Shifting the interest from analysis of single molecules to large complexes and networks Effecti ...
... Massively parallel analysis of gene expression • screen an entire genome at once • find not only individual genes that differ, but groups of genes that differ. • find relative expression level differences Shifting the interest from analysis of single molecules to large complexes and networks Effecti ...
No Slide Title
... •Another advantage of chloroplast transformation is that foreign genes can be over-expressed, due to the high gene copy number, up to 100 000 compared with single-copy nuclear genes. And there does not seem to be gene-silencing and other instability that plague nuclear transformation. The gene pro ...
... •Another advantage of chloroplast transformation is that foreign genes can be over-expressed, due to the high gene copy number, up to 100 000 compared with single-copy nuclear genes. And there does not seem to be gene-silencing and other instability that plague nuclear transformation. The gene pro ...
microarrays1
... Microarrays Massively parallel analysis of gene expression • screen an entire genome at once • find not only individual genes that differ, but groups of genes that differ. • find relative expression level differences Shifting the interest from analysis of single molecules to large complexes and net ...
... Microarrays Massively parallel analysis of gene expression • screen an entire genome at once • find not only individual genes that differ, but groups of genes that differ. • find relative expression level differences Shifting the interest from analysis of single molecules to large complexes and net ...
General
... tools KEGG does not predict presence or absence of pathways KEGG lacks pathway hole filler, operon predictor Curation tools KEGG does not distribute curation tools No ability to customize pathways to the organism Pathway Tools schema much more comprehensive Visualization and analysis ...
... tools KEGG does not predict presence or absence of pathways KEGG lacks pathway hole filler, operon predictor Curation tools KEGG does not distribute curation tools No ability to customize pathways to the organism Pathway Tools schema much more comprehensive Visualization and analysis ...
Pharmacogenomics
... genome sequence was announced in June 2000, with analyses published in February 2001. - An important feature of this project is the federal government's long-standing dedication to the transfer of technology to the private sector. By licensing technologies to private companies and awarding grants fo ...
... genome sequence was announced in June 2000, with analyses published in February 2001. - An important feature of this project is the federal government's long-standing dedication to the transfer of technology to the private sector. By licensing technologies to private companies and awarding grants fo ...
Senescence
... • Clonal reproduction of plants, using cuttings or other culturing techniques, mastered by humans for millennia (e.g. bananas) ...
... • Clonal reproduction of plants, using cuttings or other culturing techniques, mastered by humans for millennia (e.g. bananas) ...
genetics_bootcamp_tolstorukov
... Raw data processing to obtain enrichment profile Visualization of the data Finding enriched regions and/or peaks Feature analysis: –Calculating average profiles for different regions of interest (gene, intergenic regions, exons, etc.) –Analysis of the profiles for different genome regions an ...
... Raw data processing to obtain enrichment profile Visualization of the data Finding enriched regions and/or peaks Feature analysis: –Calculating average profiles for different regions of interest (gene, intergenic regions, exons, etc.) –Analysis of the profiles for different genome regions an ...
The Universal Genetic Code - Willimon-PHS
... • Karyotype – photograph of arranged chromosomes from a cell • Patient karyotype compared to normal karyotype to determine presence of genetic diseases DNA fingerprinting – technique that creates a pattern of DNA fragments • Used to identify individual organisms or compare individuals • Procedure 1. ...
... • Karyotype – photograph of arranged chromosomes from a cell • Patient karyotype compared to normal karyotype to determine presence of genetic diseases DNA fingerprinting – technique that creates a pattern of DNA fragments • Used to identify individual organisms or compare individuals • Procedure 1. ...
Genetic Engineering
... A. Selective Breeding – allowing only those individuals with desired characteristics to produce the next generation 1. Inbreeding – cross two of the same type of individual to preserve the characteristics (Risky!) 2. Cross-breeding / Hybridization – cross two different types of individuals to get th ...
... A. Selective Breeding – allowing only those individuals with desired characteristics to produce the next generation 1. Inbreeding – cross two of the same type of individual to preserve the characteristics (Risky!) 2. Cross-breeding / Hybridization – cross two different types of individuals to get th ...
Molecular Diagnostics
... The four main mechanisms by which microorganisms exhibit resistance to antimicrobials are: 1. Drug inactivation or modification: e.g. enzymatic deactivation of Penicillin G in some penicillin-resistant bacteria through the production of β-lactamases. 2. Alteration of target site : e.g. alteration of ...
... The four main mechanisms by which microorganisms exhibit resistance to antimicrobials are: 1. Drug inactivation or modification: e.g. enzymatic deactivation of Penicillin G in some penicillin-resistant bacteria through the production of β-lactamases. 2. Alteration of target site : e.g. alteration of ...
lecture 9
... ¾ COG specific phylogenetic profiles can be used to predict functional association of among COGs, and the living styles of ...
... ¾ COG specific phylogenetic profiles can be used to predict functional association of among COGs, and the living styles of ...
presentation source
... prokaryotes, that do not have a cell nucleus, and eukaryotes, which do. Prokaryotes fall into two major groups: Eubacteria and Archaea. Phenotypically, eubacteria and archaea are very similar to each other. However, it has been demonstrated by using molecular data that archaea are more closely relat ...
... prokaryotes, that do not have a cell nucleus, and eukaryotes, which do. Prokaryotes fall into two major groups: Eubacteria and Archaea. Phenotypically, eubacteria and archaea are very similar to each other. However, it has been demonstrated by using molecular data that archaea are more closely relat ...
The Dinosaur Heresies
... The number of duplication events since the common ancestor The average number of gene sequence changes since the common ancestor ...
... The number of duplication events since the common ancestor The average number of gene sequence changes since the common ancestor ...
Genomics of the evolutionary process
... strong biases in dinucleotide content [22]. This implies that a better-fitting model would accommodate these neighboring base effects, and could not be represented by a simple matrix of transitions from one single base to the next. One particular class of neighboring-base effect is the direct empiri ...
... strong biases in dinucleotide content [22]. This implies that a better-fitting model would accommodate these neighboring base effects, and could not be represented by a simple matrix of transitions from one single base to the next. One particular class of neighboring-base effect is the direct empiri ...
lesson 3 domains and binomial
... different from one another, or if they could possibly be used together. ...
... different from one another, or if they could possibly be used together. ...
forensics - bayo2pisay
... created a “rape kit”- Sexual Assault Investigation Kit for the Collection of Biological Samples ...
... created a “rape kit”- Sexual Assault Investigation Kit for the Collection of Biological Samples ...
Genetics of prokaryotic organisms
... synthesizes a new strand at the same time. The second strand is also synthesized in the acceptor cell. Then there is recombination between donor and acceptor parts of the chromosome and excision and elimination of ...
... synthesizes a new strand at the same time. The second strand is also synthesized in the acceptor cell. Then there is recombination between donor and acceptor parts of the chromosome and excision and elimination of ...
Supplementary Methods
... A total of 348 individuals of U. minor and 92 individuals of U. glabra were used for the analysis of chloroplast diversity. Most of these samples come from ex situ collections from France (Cemagref, Eric Collin), Italy (CNR, Alberto Santini) and Spain (DGB, Salustiano Iglesias). Additional field sam ...
... A total of 348 individuals of U. minor and 92 individuals of U. glabra were used for the analysis of chloroplast diversity. Most of these samples come from ex situ collections from France (Cemagref, Eric Collin), Italy (CNR, Alberto Santini) and Spain (DGB, Salustiano Iglesias). Additional field sam ...
Supplemental Data Methods
... the procedure of Rudi and coworkers (2, 3). For this, 5 l PCR product (50 ng) was added to 1 x Thermosequenase reaction buffer, 1 pmol of 5’-6-FAM labeled probe (5’-AGC TTT TTG TGA GGG TCA G-3’) (Eurogentec), TAMRAddUTP (4 pmol/L) or TAMRA-ddGTP (0.4 pmol/l) (Perkin Elmer), ROX reference dye (1 ...
... the procedure of Rudi and coworkers (2, 3). For this, 5 l PCR product (50 ng) was added to 1 x Thermosequenase reaction buffer, 1 pmol of 5’-6-FAM labeled probe (5’-AGC TTT TTG TGA GGG TCA G-3’) (Eurogentec), TAMRAddUTP (4 pmol/L) or TAMRA-ddGTP (0.4 pmol/l) (Perkin Elmer), ROX reference dye (1 ...
Various Career Options Available
... Alignment of Multiple Sequences Extending Dynamic Programming to more sequences –Dynamic programming can be extended for more than two –In practice it requires CPU and Memory (Murata et al 1985) – MSA, Limited only up to 8-10 sequences (1989) –DCA (Divide and Conquer; Stoye et al., 1997), 20-25 seq ...
... Alignment of Multiple Sequences Extending Dynamic Programming to more sequences –Dynamic programming can be extended for more than two –In practice it requires CPU and Memory (Murata et al 1985) – MSA, Limited only up to 8-10 sequences (1989) –DCA (Divide and Conquer; Stoye et al., 1997), 20-25 seq ...
Molecular taxonomy,use of modern methods in the identification of a
... identification which involves process of determining a particular isolate belonging to a recognized taxon. A species is a group of interbreeding natural populations that is reproductively isolated from other such groups. To identify all such types that lived or exist on this earth planet, several cr ...
... identification which involves process of determining a particular isolate belonging to a recognized taxon. A species is a group of interbreeding natural populations that is reproductively isolated from other such groups. To identify all such types that lived or exist on this earth planet, several cr ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.