A molecular phylogeny of enteric bacteria and implications for a
... clearly seen as gene pools, or genome pools, at the DNA sequence level. We argue from these data that, for at least the enteric bacteria, bacterial species do exist and can be defined in similar ways with both phenotypic and genotypic data. ...
... clearly seen as gene pools, or genome pools, at the DNA sequence level. We argue from these data that, for at least the enteric bacteria, bacterial species do exist and can be defined in similar ways with both phenotypic and genotypic data. ...
CTEGD Symposium, UGA, Athens, May 2011
... latest genomic-scale datasets including complete genome sequences, annotations, and functional genomics such as proteomics, microarray, RNA-Seq, ChIp-chip, SAGE and EST data. The specific advantage of the EuPathDB databases lies in the graphical search interface that allows users to combine datasets ...
... latest genomic-scale datasets including complete genome sequences, annotations, and functional genomics such as proteomics, microarray, RNA-Seq, ChIp-chip, SAGE and EST data. The specific advantage of the EuPathDB databases lies in the graphical search interface that allows users to combine datasets ...
Bos, C.J. ... strated that parasexual mechanisms occur in
... The original wild type has black conidiospores on rather long conidiophores. A mutant with low conidiophores was isolated and from this strain (N402) we derived auxotrophic and color mutants. Complementation tests are in progress and different genes are being mapped by haploidization of heterozygous ...
... The original wild type has black conidiospores on rather long conidiophores. A mutant with low conidiophores was isolated and from this strain (N402) we derived auxotrophic and color mutants. Complementation tests are in progress and different genes are being mapped by haploidization of heterozygous ...
pdf
... case) and extracting the entirety of the DNA into a test tube (on the order of 1010 base pairs (bps)). The DNA is then cut randomly into about equal pieces (we assume a normal distribution with known mean and variance for each experiment, average sizes are 200bps to 5000bps). These pieces of DNA are ...
... case) and extracting the entirety of the DNA into a test tube (on the order of 1010 base pairs (bps)). The DNA is then cut randomly into about equal pieces (we assume a normal distribution with known mean and variance for each experiment, average sizes are 200bps to 5000bps). These pieces of DNA are ...
supplementary materials and methods
... Analysis of the LMNB1 duplication by quantitative real-time PCR. Amplification was performed in a total of 20 µl containing 10 µl of Taqman Universal PCR Master mix (P/N 4324018, Applied Biosystems), 1 µl of RNase P kit (20X, VIC dye, P/N 4316844), 2 µl of forward (5’-gccaaaaaacagttagcagatgaa) and r ...
... Analysis of the LMNB1 duplication by quantitative real-time PCR. Amplification was performed in a total of 20 µl containing 10 µl of Taqman Universal PCR Master mix (P/N 4324018, Applied Biosystems), 1 µl of RNase P kit (20X, VIC dye, P/N 4316844), 2 µl of forward (5’-gccaaaaaacagttagcagatgaa) and r ...
THE CHASM BETWEEN THE HUMAN AND CHIMPANZEE GENOMES
... A major problem with this type of selective analysis is that nearly all of the entire genome is now believed to be functional, as stated in the recent ENCODE project consortium reports (2012). The non-coding regions have been shown to provide many critical control features and nucleotide templates ( ...
... A major problem with this type of selective analysis is that nearly all of the entire genome is now believed to be functional, as stated in the recent ENCODE project consortium reports (2012). The non-coding regions have been shown to provide many critical control features and nucleotide templates ( ...
Epilepsy genetics update 080916
... – NGS panel – Single gene targeted test in Dravet, MPSI or epilepsy-aphasia syndromes….may only be available as part of panel! ...
... – NGS panel – Single gene targeted test in Dravet, MPSI or epilepsy-aphasia syndromes….may only be available as part of panel! ...
Genome-based bioprospecting of microbes for new
... made the ‘genomic snapshot’ approach obsolete, providing almost complete genome sequences available for mining of genes of interest in a matter of weeks. The task of mining remained, however, quite laborious, until the development of a software antiSMASH [3], that allows to efficiently detect seco ...
... made the ‘genomic snapshot’ approach obsolete, providing almost complete genome sequences available for mining of genes of interest in a matter of weeks. The task of mining remained, however, quite laborious, until the development of a software antiSMASH [3], that allows to efficiently detect seco ...
7. Recombinant DNA Vectors
... artificial chromosome vectors (BACs, PACs, YACs)--cloning chromosomal regions b. Conventional E. coli plasmid cloning vectors typically have: origin of replication that functions in bacteria antibiotic resistance gene(s) selectable marker gene (often lacZ, encoding beta-galactosidase) polylinker (al ...
... artificial chromosome vectors (BACs, PACs, YACs)--cloning chromosomal regions b. Conventional E. coli plasmid cloning vectors typically have: origin of replication that functions in bacteria antibiotic resistance gene(s) selectable marker gene (often lacZ, encoding beta-galactosidase) polylinker (al ...
Determining Evolutionary Relationships Using BLAST
... bioinformatics, entire genomes can be quickly compared in order to detect genetic similarities. The most powerful bioinformatics tool currently in use is BLAST which stands for Basic Local Alignment Search Tool. Using BLAST, you can input a gene sequence of interest and search entire genomic librari ...
... bioinformatics, entire genomes can be quickly compared in order to detect genetic similarities. The most powerful bioinformatics tool currently in use is BLAST which stands for Basic Local Alignment Search Tool. Using BLAST, you can input a gene sequence of interest and search entire genomic librari ...
The Microbial World and You
... Immunology, the study of immunity, expanded rapidly in the twentieth century. Smallpox was eliminated, and many new vaccines became available. A major challenge now will be to defeat the AIDS virus, which attacks the immune system. In 1933, Rebecca Lancefield proposed an immunologically based classi ...
... Immunology, the study of immunity, expanded rapidly in the twentieth century. Smallpox was eliminated, and many new vaccines became available. A major challenge now will be to defeat the AIDS virus, which attacks the immune system. In 1933, Rebecca Lancefield proposed an immunologically based classi ...
Persönliche PDF-Datei für R. Augustin, TCG Bosch
... in any of these partners can compromise the health of the whole animal [25]. Because Hydra have been cultivated tens of years under standard conditions at constant temperature and identical food, it came as a surprise that examinations of the microbiota in different species kept in the laboratory fo ...
... in any of these partners can compromise the health of the whole animal [25]. Because Hydra have been cultivated tens of years under standard conditions at constant temperature and identical food, it came as a surprise that examinations of the microbiota in different species kept in the laboratory fo ...
DNA Technology and Genomes
... impact, unknown effect on other organisms, loss of flora and fauna biodiversity Access and Intellectual Property: domination of world food production by few companies; increased dependence on industrial nations by developing countries; Ethics: violation of natural organisms’ intrinsic values; tamper ...
... impact, unknown effect on other organisms, loss of flora and fauna biodiversity Access and Intellectual Property: domination of world food production by few companies; increased dependence on industrial nations by developing countries; Ethics: violation of natural organisms’ intrinsic values; tamper ...
DUAL TRAFFICKING PATHWAYS OF CONNEXINS TO GAP …
... • Conjugation and the recovery of gene replacement clones are efficient, so that many replicate clones are obtained for phenotypic testing • With one insertion per 280 bp, phenotypic analysis of several independent insertions in a given gene obviates the need for linkage analysis ...
... • Conjugation and the recovery of gene replacement clones are efficient, so that many replicate clones are obtained for phenotypic testing • With one insertion per 280 bp, phenotypic analysis of several independent insertions in a given gene obviates the need for linkage analysis ...
CH24
... Evolutionary relationships among organisms (at the DNA level) are summarized in phylogenetic trees, or phylogenies (branching diagram). All organisms on earth have descended from a common ancestor. A phylogeny that shows only the relationships among organisms is an unrooted tree. A phylogeny tha ...
... Evolutionary relationships among organisms (at the DNA level) are summarized in phylogenetic trees, or phylogenies (branching diagram). All organisms on earth have descended from a common ancestor. A phylogeny that shows only the relationships among organisms is an unrooted tree. A phylogeny tha ...
Phylogenomics: improving functional predictions for uncharacterized
... methods will be more likely than phylogenomic methods to make inaccurate functional predictions (see Table 4). Another major advantage of phylogenetic methods over most similarity methods comes from the process of masking (see above). For example, a deletion of a large section of a gene in one speci ...
... methods will be more likely than phylogenomic methods to make inaccurate functional predictions (see Table 4). Another major advantage of phylogenetic methods over most similarity methods comes from the process of masking (see above). For example, a deletion of a large section of a gene in one speci ...
Dr. McKay`s lecture
... comprehensively studied anatomy and developmental program • Sequenced genome ...
... comprehensively studied anatomy and developmental program • Sequenced genome ...
Supplementary Information (doc 33K)
... indicator I(i,j) = 1 if a sample i carries a CNV overlapping at least one gene in gene-set j, and zero otherwise. The total I(i,j) score for gene set j is obtained by summing over i. For testing, this sum is partitioned between cases and controls, shown in the column of the number of sample. The num ...
... indicator I(i,j) = 1 if a sample i carries a CNV overlapping at least one gene in gene-set j, and zero otherwise. The total I(i,j) score for gene set j is obtained by summing over i. For testing, this sum is partitioned between cases and controls, shown in the column of the number of sample. The num ...
Molecular Contributions to the Construction of the Human Phylogeny
... and Eberhardt (2008) found that when race is portrayed as a biologically supported concept, it allows people to view a racial outgroup as unrelated to themselves. This was not necessarily due to preconceived prejudice; rather, the idea that another race was biologically distinct produced a feeling o ...
... and Eberhardt (2008) found that when race is portrayed as a biologically supported concept, it allows people to view a racial outgroup as unrelated to themselves. This was not necessarily due to preconceived prejudice; rather, the idea that another race was biologically distinct produced a feeling o ...
How do we know that DNA carries genetic information?
... 1978: The first human gene is cloned – insulin 1982: Genetically-engineered insulin is approved for use 1985: PCR (Polymerase Chain Reaction) is invented ...
... 1978: The first human gene is cloned – insulin 1982: Genetically-engineered insulin is approved for use 1985: PCR (Polymerase Chain Reaction) is invented ...
Hongbin (H.-B.) Zhang, Ph.D. - Department of Soil and Crop Sciences
... families varied significantly not only within a species, but also within a bi-parental population; and that the genetics and variation of a family size is subject to natural and artificial selection, and associated with biology. Recent studies revealed that a majority of genes and nongene elements i ...
... families varied significantly not only within a species, but also within a bi-parental population; and that the genetics and variation of a family size is subject to natural and artificial selection, and associated with biology. Recent studies revealed that a majority of genes and nongene elements i ...
An Exceptional Gene: Evolution of the TSPY Gene Family
... 1.2. Ape TSPY Genes Two factors limit comparisons between human TSPY genes and those of other apes. First, some reference sequences, such as that of the gorilla, have been derived from females and thus provide no information about Y-specific genes such as TSPY. Second, although early studies had rev ...
... 1.2. Ape TSPY Genes Two factors limit comparisons between human TSPY genes and those of other apes. First, some reference sequences, such as that of the gorilla, have been derived from females and thus provide no information about Y-specific genes such as TSPY. Second, although early studies had rev ...
Science 9 – Biological Diversity Unit Review
... but experts also say we need to maintain biological diversity in all countries, including Canada. When should these concerns be addressed only by the citizens of each country, and when should they be addressed globally? Support your answer with facts and/or examples. ...
... but experts also say we need to maintain biological diversity in all countries, including Canada. When should these concerns be addressed only by the citizens of each country, and when should they be addressed globally? Support your answer with facts and/or examples. ...
The Genetics and Prevention of Sudden Cardiac Death
... TAAD and Marfan syndrome panel (11 genes) ...
... TAAD and Marfan syndrome panel (11 genes) ...
Tools of Genetic Engineering 2
... Size fractionation of DNA (into about 10 Kb or more in size) to be cloned is important in approaching the maximum cloning capacity of the vector used. However, DNA fragments of a size not suitable for cloning will be ligated to vector and will lower the efficiency of introduction of the recombinant ...
... Size fractionation of DNA (into about 10 Kb or more in size) to be cloned is important in approaching the maximum cloning capacity of the vector used. However, DNA fragments of a size not suitable for cloning will be ligated to vector and will lower the efficiency of introduction of the recombinant ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.