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I. Microbial Genetics (Chapter 7) A. Overview 1. all of the information
I. Microbial Genetics (Chapter 7) A. Overview 1. all of the information

... 3. DNA helix unravels and actual replication occurs at the replication fork a. bidirectional, replicons (portion of genome containing an origin and replicated as a unit) separate when forks meet opposite the origin b. replication fork and associated enzymes may be attached to plasma membrane 4. euc ...
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chapter15_Sections 5

... • Genetic engineering produces a genetically modified organism (GMO) • A gene may be altered and reinserted into an individual of the same species • A gene from one species may be transferred to another to produce an organism that is transgenic ...
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... Homozygous for the mutant allele: Make abnormally thick and sticky mucous leading to lung congestion and blockage of the ...
The Genetics of Microorganisms
The Genetics of Microorganisms

... • The small subunit binds to the 5’ end of the mRNA • Large subunit supplies enzymes for making peptide bonds on the protein • The ribosome scans the mRNA by moving in the 5’ to 3’ direction along the mRNA • The first codon is the START codon (AUG but can rarely be GUG) • With the mRNA message in p ...
Genetics
Genetics

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Domain Three (3_genetics)

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DNA (Gene) Mutations
DNA (Gene) Mutations

... Change in the nucleotide sequence of a gene May only involve a single nucleotide May be due to copying errors, chemicals, viruses, etc. ...
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3.1 Genetics

... • A GENE is a small segments of DNA found at specific places on a chromosome that code for a protein • genes can vary in length from 100s to 1000s of BASES • the arrangement of bases will decide what kind of protein is produced e.g. ACCATAGG  make protein “A” AGGCGTTA  make protein “B” ...
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... Genes are the units of heredity discovered by Gregor Mendel more than a century ago. They contain the recipes, or instructions, for making the proteins of which all living things, from bacteria to humans, are built and which all organisms use to carry out their functions. Since the 1970s, scientists ...
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No Slide Title

... „ For the first time in human history we can produce a high-resolution picture of our individual genomes and monitor for changes in diseases „ For the first time the role of genetic and life-style risk factors can be defined „ Special European competitive advantage of in biomedical research can be u ...
Chromosomal Mapping of Murine c-fes and c
Chromosomal Mapping of Murine c-fes and c

... Polymorphism within the murine c-fes locus was detected by Southern blotting of restriction endonuclease-digested DNA of the two progenitor strains A and B, followed by hybridization with a c-fes probe (7). The pattern obtained by using DNA digested with four different restriction enzymes suggests t ...
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INF115 Compulsory Exercise 2 A genome is the term

... example "4­17" means cabin 17 on deck 4. The cabins are assigned to different price categories  depending on the number of beds and location on the ship. The system must also store  information about the passengers and their reservations. Every passenger gets a unique email  address, name, gender, d ...
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ab initio - Ware Lab

... The Maize Genome Sequencing Consortium was launched with a three-year grant from NSF to produce a complete sequence of the maize (B73) genome. At 2.5 Gb, the maize genome rivals mammalians in terms of size, and is six times larger than rice, owing to its high content of retrotransposable elements. T ...
Tutorial DNA - UniMAP Portal
Tutorial DNA - UniMAP Portal

... complementary structure of the two strands: Adenine and guanine in one strand bond with thymine and cytosine, respectively, in the other. DNA replication is a simple concept - a cell separates the two original strands and uses each as a template for the synthesis of a new complementary strand. Biolo ...
press alert - the Gregor Mendel Institute
press alert - the Gregor Mendel Institute

... the diploid central cell (the companion cell of the egg) to form the triploid placenta-like endosperm that nourishes the embryo, while the other fertilizes the haploid egg to form the diploid embryo. DNA glycosylase enzymes catalyze active DNA de-methylation in plants. The Arabidopsis thaliana DEMET ...
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Lecture#3 Genes encode Proteins Readings: Problems: Concepts

... Note: the entire model was inferred from the properties of the mutants (phenotype) - later the presence of defective enzymes was demonstrated by independent biochemical analysis History - first insight into the function of genes and how they worked (remember it wasn't until 1944 that DNA was shown t ...
Andy Moeller – bacterial conjugation
Andy Moeller – bacterial conjugation

... were still generated. This led Marrs to hypothesize the existence of a releasable, DNAcontaining sex pilus capable of transmitting DNA from cell to cell. He was partially correct in this hypothesis, as it is now known that a sex pilus functions in bacterial conjugation. However, since a bacterium’s ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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