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Dr. Sabika Firasat - University of Wah
Dr. Sabika Firasat - University of Wah

... Endothelial dysfunction (ED) precedes the development of cardiovascular diseases which are among the leading causes of morbidity and mortality worldwide. ED is considered as an early sign of atherosclerosis and is attributed to a reduction in nitric oxide (NO) bioactivity and an increase in oxygen f ...
DNA profiling - Our eclass community
DNA profiling - Our eclass community

... RECOMBINANT DNA Recombinant DNA is used for the production of specific proteins  The first chemical produced by this mehtod was human insulin  The human gene is placed into a bacterium which can then use the genetic information to produce the human hormone.  The hormone is refined from the cultu ...
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AP Bio Ch 15

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... Too much time in the tanning booth might damage epithelial cells due to the UV radiation. the mosty likely effect would be….. ...
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Molecular Biology BCH 361

...  He though that a DNA molecule contained only four units, each unit contain phosphate-sugar-base -in order- linked together in a repeated manner, i.e. a tetranucleotide.  Furthermore, he considered such a simple sequence could not allow DNA any role in coding for anything.  This was later to be p ...
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Chapter 5 PPT Review

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Semester 2 Exam Review

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Nucleus - Maryville University

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pGLO2011 Wilkes

... transforming principle from pneumococcus. Biochemical tests revealed it to be deoxyribonucleic acid (DNA). Taken together, all this evidence pointed to DNA as the components of genes. ...
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Genetics (4) - HCC Learning Web

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A-level Biology B Question paper Unit 2 - Genes and Genetic
A-level Biology B Question paper Unit 2 - Genes and Genetic

... (c) The mRNA coding for a protein has 375 bases. The protein is secreted in an inactive form. It is activated when an enzyme removes three amino acids from one end. Calculate how many amino acids there are in the activated protein. Show your working. ...
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Are there bacterial species, and what is the goal of metagenomics

... have apparently evolved from a common ancestor with phage- and plasmid-like characteristics and belong to a family of related genetic elements. All contain homologous parts with genes found in all related islands. Within these conserved parts unrelated blocks of DNA are interspersed. By screening la ...
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Recombination and Repair

... Single crossover usually forms short-lived hybrid DNA molecules. promoter recombination of linear chromosomes. cannot cause recombination between two circular DNA molecules. Double crossovers forms recombination. ...
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PowerPoint Presentation - Lectures For UG-5

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... together with electron microscopy and a cultivation-independent metagenomic approach to characterize chemosynthetic symbionts in the trophosome tissue of Escarpia and Lamellibrachia at the MCR. 16S rRNA and ITS gene phylogenetic analysis indicated all MCR individuals harbored endosymbionts that were ...
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... 1. Summarize how restriction enzymes cut DNA. 2. Explain how restriction maps show the lengths of DNA fragments. 3. Describe the role of polymerases in copying DNA segments. 4. Outline the PCR process and explain why it is used. 5. Describe what a DNA fingerprint represents. 6. Summarize how DNA fin ...
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... Verify GMO-negative result is not due to PCR reaction not working properly ...
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Chapter 13 Mutation, DNA Repair, and Recombination

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... • Now, a complex or pleiotropic function that was performed by a single gene prior to duplication, is now subdivided into discrete components. • These copies are now all very necessary and essential, as they keep individual and unique cis-regulatory regions. ...
Genetics and Heredity
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... Baldness is an autosomal trait and is apparently influenced by sex hormones after people reach 30 years of age or older. In men the gene is dominant, while in women it is recessive. A man needs only one allele (B) for the baldness trait to be expressed, while a bald woman must be homozygous for the ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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