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Linkage analysis - Scheid Signalling Lab @ York University
Linkage analysis - Scheid Signalling Lab @ York University

... markers establishes location of disease gene • LOD score analysis, and other methods are employed • Once we know the approximate location… – The heavy molecular biology begins ...
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recBCD

... sequences in SS and DS DNA (paranemic or side-by-side structure) 3. Post-synapsis or strand-exchange: SS DNA replaces the same strand in the duplex to form a new DS DNA (requires ATP hydrolysis) ...
Unit 5 Molecular Genetics Objectives
Unit 5 Molecular Genetics Objectives

... 3 Inducers and repressors are small molecules that interact with regulatory proteins and/or regulatory sequences. 4 Regulatory proteins inhibit gene expression by binding to DNA and blocking transcription (negative control). 5 Regulatory proteins stimulate gene expression by binding to DNA and stimu ...
Burnes, P.A., J-H. Kinnaird and J.R.S Fincham* The nonsense mutant... , is suppressible by
Burnes, P.A., J-H. Kinnaird and J.R.S Fincham* The nonsense mutant... , is suppressible by

... sequences for 17S-5.8S-26S rRNA with additional flank transcribed spacer region of Neurospora ing sequences, was constructed by Free et al. in 1979. We used their clone as a probe to clone adjacent secrassa wild type strain 74A. quence. Initial cloning experiments were conducted using pBR325. A clon ...
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Microsoft Word 97

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bchm6280_16_ex1

... Using this information, answer the following questions in the form of a table that lists the accession numbers for the coding, non-coding, model and reference transcript/proteins. Attach the PDF you downloaded from this section. 1. How many Refseq protein-coding transcripts (with prefex NM) are list ...
Table 7. Summary statistics for the consensus gene set of Haliotis
Table 7. Summary statistics for the consensus gene set of Haliotis

... from the distinctive patterns of repeat element expansion between the two species and the increased genome size of H.discus hannai may be associated with the non-LTR elements (especially LINE/I) contribution, in parallel to the human genome[23]. Genes were predicted through three different algorithm ...
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... • Chromosomes (and genes) occur in pairs • New combinations of genes occur in sexual ...
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... Copy each of these sequences either into an excel sheet or directly into ApE (see description in 8a where you use “Find” and highlight options. At this point go to Step 3 and Click on GBrowse (green rectangle) to get the gene sequence from the short chromosome and follow the same procedure upto Ste ...
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Biologists have learned to manipulate DNA

... 2. Help us understand how our genes work from others 13.2 Biologists can engineer bacteria to make useful products I. Engineering bacteria: an introduction A. Plasmids are small circle-shape DNA molecule separate from larger bacterial chromosomes B. Plasmids can be shared between bacteria, for examp ...
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Chromosomes in prokaryotes

... Fertility-F-plasmid which contain tra-genes (transfer). They are capable of conjugation and help bacteria produce pili. Resistance- R-plasmids, which contain genes that can build a resistance against antibiotics or poisons. Col-plasmids, which contain genes that determine the production of bacterioc ...
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PowerPoint 演示文稿

... 7 The natural incidence of mutations is increased by mutagens. Mutations may be concentrated at hotspots. A type of hotspot responsible for some point mutations is caused by deamination of the modified base 5-methylcytosine. 8 Forward mutations occur at a rate of ~10 6 per locus per generation; bac ...
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... cancer-fighting medicines in their eggs. The animals have had human genes added to their DNA so that human proteins are secreted into the whites of their eggs, along with complex medicinal proteins similar to drugs used to treat skin cancer and other diseases. ...
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HISAT-genotype: fast software for analyzing human genomes

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NCBI genome database - Winona State University
NCBI genome database - Winona State University

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DNA Powerpoint Notes

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Genetics Unit Organization

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... Explain the importance of DNA in cells: BIO.6 f  DNA stores the information for directing the construction of proteins within a cell. These proteins determine the phenotype of an organism.  Genetic information encoded in DNA molecules provides instructions for assembling protein molecules. The cod ...
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... • This process can only be mediated by temperate phages. • The only genes that can be transferred from the donor to the recipient are the genes that are immediately adjacent to the phage insertion site on the donor chromosome. • When the phage DNA is excised from the chromosome as the virus enters i ...
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Chapter 29 DNA as the Genetic Material Recombination of DNA

... • Messelson and Weigle showed by 13 C and 15N labeling that recombinant phage contained DNA from both “parents” ...
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Table of Contents

... • The neighborhood around the RFLP can be screened for other RFLPs. If one is linked directly, a DNA fragment from the region can be used to identify a cDNA sequence. • The gene in affected and unaffected people is compared to determine the genetic difference responsible for the disease. ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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