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Lateral gene transfer in prokaryotic genomes: which genes
Lateral gene transfer in prokaryotic genomes: which genes

... sometimes confers advantages (toxin genes in phages of C. diphteriae). The phage can later be induced to exit the chromosome and replicate (lytic ...
The Human Genome: Structure and Function of Genes
The Human Genome: Structure and Function of Genes

A research paper published in the journal Mutation Research
A research paper published in the journal Mutation Research

... The fraction of erythrocytes with mutations at the GPA gene locus is considered to be one of the indices of somatic mutations induced by ionizing radiation for estimating the degree of related genome damage and cancer risk. Previous studies of A-bomb survivors revealed that erythrocyte GPA mutations ...
An Artist in Gene Editing - Max-Planck
An Artist in Gene Editing - Max-Planck

... if it attacks again. They can cut through it, incapacitating the pathogens. In this way, the CRISPR-Cas9 system provides the bacterial immune system with a kind of memory. RNA: The DNA molecule contains the assembly instructions for all proteins in an organism. These instructions aren’t translated d ...
AP Biology
AP Biology

...  Restriction fragment analysis detects DNA differences that affect restriction sites (pp. 383-386, FIGURES 20.820.10) Gel electro-phoresis makes it possible to separate and isolate DNA restriction fragments of different lengths. Restriction fragment length polymorphisms (RFLPs) are differences in D ...
Interpolated Markov Models for Gene Finding
Interpolated Markov Models for Gene Finding

... •  signals: the sequence signals (e.g. splice junctions) involved in gene expression •  content: statistical properties that distinguish proteincoding DNA from non-coding DNA •  conservation: signal and content properties that are conserved across related sequences (e.g. orthologous regions of the m ...
Richard Dawkins on the nature of the gene
Richard Dawkins on the nature of the gene

... “I am using the word gene to mean a genetic unit that is small enough to last for a large number of generations ... This is not a rigid all-or-nothing definition but a kind of fadingout definition, like the definition of ‘big’ or ‘old’. The more likely a length of chromosome is to be split by crossi ...
Genes: Structure, Replication, & Mutation
Genes: Structure, Replication, & Mutation

... Forward mutation: A mutation in the wild type, causing some notable change in phenotype Reversion mutation: A change causing a mutant to appear to revert back to the wild type phenotype Back mutation: A reverse mutation in which the mutant nucleotide sequence has truly reverted back to exactly its o ...
Know Your Chromosomes -R-ES-O-N-A-N-C-E-.-I-J-u-ne--1-99
Know Your Chromosomes -R-ES-O-N-A-N-C-E-.-I-J-u-ne--1-99

... in enzyme E1 and human cells defective in enzyme E2 are chosen as parent cells. Hybrid cells grow in the special growth medium provided they have enzyme E1 coded by the human chromosome along with the complete complement of the mouse genome. Thus one concludes that the human chromosome retained in t ...
Fusion gene detection
Fusion gene detection

Cells and DNA Table of Contents
Cells and DNA Table of Contents

... Every person has two copies of each gene, one inherited from each parent. Most genes are the same in all people, but a small number of genes (less than 1 percent of the total) are slightly different between people. Alleles are forms of the same gene with small differences in their sequence of DNA ba ...
Provincial Exam Questions
Provincial Exam Questions

... Unit: Cell Biology: DNA Replication (B5 & B6) ...
Biotechnology-
Biotechnology-

... Course objective: Students will be able to explain major methods and techniques used in molecular genetics to isolate, recombine, amplify, find and study genes of interest. Necessary for future material on: last five genetics labs. Helpful for Directed Studies and Internships in research labs. In ge ...
File
File

... Outline the use of polymerase chain reaction (PCR) to copy and amplify minute quantities of DNA. State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. State that gel electrophoresis of DNA is used in DNA profiling. Describe the appl ...
Fulltext PDF
Fulltext PDF

... proposed their famous double helix model for the DN A structure. N ow specific questions about the mechanistic aspects of a gene's function like their duplication, control of phenotypic characters, mutation and recombination and other properties could be asked. As is often stated, asking the right q ...
Figure 1-2
Figure 1-2

Supplementary Information
Supplementary Information

... primers pPlatII-A_SpeI and pPlatII-I_SacI. This fragment was digested with SpeI and SacI, and ligated with the fragment containing the replication ori without Esp3I sites, which was prepared from pDONR207 by PCR using primers pPlatII-J_SacI and pPlatII-D_XbaI. The platinum TALEN genes containing HD, ...
the 3
the 3

... are easier to make and contain all the genome sequences. Angelia 09 ...
DNA methylation
DNA methylation

... The molecular basis of epigenetics is complex. It involves modifications of the activation of certain genes, but not the basic structure of DNA. Additionally, the chromatin proteins associated with DNA may be activated or silenced. This accounts for why the differentiated cells in a multi-cellular ...
Final exam review 4
Final exam review 4

... 12. Briefly describe the work of the scientists pg 196. 13. Thoroughly describe the process of DNA replication 14. Describe all key terms page 201. Chapter 10 Know EVERYTHING in this chapter! Review activity- Do this now! 15. Write in paragraph form: How do proteins get made from DNA? Thoroughly des ...
the history of genetics
the history of genetics

... to represent recessive traits. Figure 1.2 uses this system to demonstrate Mendel’s law of segregation. The pure red sweet pea and the pure white sweet pea each have two genes—RR for the red and rr for the white. The possible outcomes of this mating in the first generation are all hybrid (a combinati ...
7. According to Dr. Malcolm (guy in black leather jacket), “Dinosaurs
7. According to Dr. Malcolm (guy in black leather jacket), “Dinosaurs

... People also worry about what effects genetically modified crops will have on humans. Again, there are many different ideas on how humans might be affected. Some genetically modified plants are created with genes for antibiotic resistance. Some people are concerned that these genes may be passed alon ...
PRE-CONFERENCE SYMPOSIA SESSIONS Topical symposium
PRE-CONFERENCE SYMPOSIA SESSIONS Topical symposium

... and industry, a new reference genome of the Chinese Hamster was generated using PacBio sequencing, to overcome the drawbacks of the available, Illumina-based reference draft genomes (large number of contigs and scaffolds, high percentage of NNNs, some genes split across scaffolds/contigs, difficult ...
21_Lecture_Presentation_PC
21_Lecture_Presentation_PC

... • Number of genes is not correlated to genome size • For example, it is estimated that C. elegans has 100 Mb and 20,000 genes, while Drosophila has 165 Mb and 13,700 genes • Vertebrate genomes can produce more than one polypeptide per gene because of alternative splicing of RNA transcripts ...
DNA Recombination Mechanisms
DNA Recombination Mechanisms

... We know it exists because UvrA- and RecAcells are much more sensitive to UV than cells containing only one mutation ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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