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ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

What`s New in Swine Molecular Biology
What`s New in Swine Molecular Biology

... (Moller et al. 1996), we also found that ~80% of the high glycogen pork products were from the commercial white pig population and not the purebred Hampshire pig population as previously assumed. The frequency of the RN gene mutation probably arose in the pig population because it can increase the ...
1. Genes and Genetic Engineering (v2.1)
1. Genes and Genetic Engineering (v2.1)

...  screen embryos for genetic diseases – this is called pre-implantation genetic diagnosis (PGD)  screen embryos for the right number of chromosomes – this is called pre-implantation genetic screening (PGS)  screen embryos for their sex – some genetic diseases only affect boys, and in the UK, paren ...
SEGMENTAL VARIATION
SEGMENTAL VARIATION

... Regions that are deleted or duplicated should yield lesser or greater numbers of reads •Detection of breakpoints by: –Short paired reads (like Illumina paired-end sequencing) Are the sequences at two ends of a fragment both from the same chromosome? Are they the right distance apart? –Long reads (kb ...
Topic 3 powerpoint notes
Topic 3 powerpoint notes

... sickle cell ______ and do show some signs of the disease but not nearly as severe. • Having one or two sickle cell alleles prevents ________. ...
PPT 2.1M - CytoMaize.ORG
PPT 2.1M - CytoMaize.ORG

... Mutation: 1) The act or process of making a heritable change in the genetic material (DNA). Phenotype: 2) The appearance of an individual. Phenotypes can be normal (wild-type) or mutant. A mutant individual can have parents that are genetic carriers, but show a normal phenotype. Mutant phenotypes a ...
Fundamentals of Biotechnology
Fundamentals of Biotechnology

... Allows for the function of the KO’d gene to be deduced from the defects seen in the mice can be used to mimick some disease Unlike traditional transgenics the trangene is targeted to a specific site in the DNA of the mouse ...
An intron nucleotide sequence variant in a
An intron nucleotide sequence variant in a

... excision is insufficient to predict whether or not this particular G-»A replacement could cause ineffective pre-mRNA processing. In two studies insertions or deletions made in intron sequences had no apparent effect on gene transcription and processing to RNA, implying that some intron sequences are ...
dilemmas regarding clinical obligation
dilemmas regarding clinical obligation

... Although carriers of mutations resulting in autosomal recessive disorders are not usually affected phenotypically, nor are they symptomatic, identifying heterozygous deletions for genes in which homozygous deletions have clinical consequences has merit. For example, identification of carrier status ...
Insulin sensitivity
Insulin sensitivity

... values were co-twin normalized, which involved dividing the obese twin’s expression values with that of those of the thin co-twin in order to correct for the identical genetic back-ground. ...
BioSc 231 Exam 1 2008
BioSc 231 Exam 1 2008

... Resistance to a popular herbicide is a dominant trait in soybean. Assume that researchers at a major seed company conducted a series of experiments where herbicide resistant plants were crossed with plants sensitive to the herbicide. The following progeny were produced: 325 resistant plants and 350 ...
SEGMENTAL VARIATION
SEGMENTAL VARIATION

... Regions that are deleted or duplicated should yield lesser or greater numbers of reads •Detection of breakpoints by: –Short paired reads (like Illumina paired-end sequencing) Are the sequences at two ends of a fragment both from the same chromosome? Are they the right distance apart? –Long reads (kb ...
Simple methods for isolating homoeologous loci from allopolyploid genomes
Simple methods for isolating homoeologous loci from allopolyploid genomes

Regulating Evolution - Nicolas Gompel`s lab
Regulating Evolution - Nicolas Gompel`s lab

... The expression of a gene entails the transcription of the DNA sequence into a messenger RNA (mRNA) version and the translation of that mRNA into a protein sequence. The expression of most genes is regulated at the transcriptional level— cells do not waste energy making ­mRNAs and proteins they do no ...
Biotechnologies Influencing Agriculture: Molecular
Biotechnologies Influencing Agriculture: Molecular

... 1988 : - putting the foreign genes into chloroplast genome Late 1990 : several biotech companies have initiated major programmes on chloroplast transformation 1998 : Chloroplast transformation has been touted at least as far back as 1998 as a means of “containing” transgenes; that is, preventing the ...
CRISPR germline engineering—the community
CRISPR germline engineering—the community

... Katrine S. Bosley is at Editas Medicine, Cambridge, Massachusetts, USA; Michael Botchan is in the Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA; Annelien Bredenoord is in the Department of Medical Humanities, University Medical Center, Utrech ...
Extended Inheritance and Developmental Niche Construction: from
Extended Inheritance and Developmental Niche Construction: from

... their molecular, biochemical and intrinsic function to their cellular, constituent, component or contextual function due to their recruitment and organization in complex cellular networks. In other words, all agents involved in the regulation of gene ...
bio12_sm_07_5
bio12_sm_07_5

... chromosomes. This has evolutionary significance because a gene can be retained in the original site and duplicate sites can undergo mutations that may be selected for by nature. If the mutations are advantageous, they will be passed on to further generations of the species. If the mutations are not ...
Mutation Study Guide
Mutation Study Guide

... A chromosomal mutation typically affects more genes because it takes place at a chromosomal level. Chromosomal mutations can have a large effect and may result in a disrupted gene or abnormal regulation of genes. 6. What is translocation? The attachment of a piece of one chromosome to a non-homologo ...
1 - LWW.com
1 - LWW.com

... DNA probe obtained from Ventana Medical Systems Inc (Tucson, AZ) according to manufacturer’s instructions and using the Benchmark XT automated slide stainer with appropriate secondary and ultraView SISH Detection reagents. Following precipitation of the silver particles within the nuclei, a single b ...
Mutations - Fulton County Schools
Mutations - Fulton County Schools

... structure of genetic material of an organism  Mutations can be in DNA or can be chromosomal  Mutations can happen more than once in a sequence [and typically do]  Causes:  mutagens – radiation or chemical substances that increase the rate of mutations ...
Pierce5e_ch19_lecturePPT
Pierce5e_ch19_lecturePPT

... a gene that encodes the phenotype • Reverse genetics: Begins with a gene of unknown function, first inducing mutations and then checking the effect of the mutation on the phenotype ...
ChimPipe Documentation Release v0.8.0 Bernardo Rodríguez-Martín, Emilio Palumbo and Sarah Djebali
ChimPipe Documentation Release v0.8.0 Bernardo Rodríguez-Martín, Emilio Palumbo and Sarah Djebali

... • Cancer genomics. It is very well know that the generation of fusion genes through chromosomal rearrangements is a major driver in certain types of cancer. These are hydrid genes formed from two previously separate genes that encode altered proteins with abnormal activity. Thus, the identification ...
TURNING PAGES
TURNING PAGES

... (Slightom et al., 1980). So, homologous recombination was very much a part of my scientific gestalt. And, not surprisingly, having worked with globin genes, I kept thinking that it ought to be possible to use DNA coding for the normal human B globin gene, which was now readily available, to correct ...
Document
Document

... (17) In the black and white gene expression image, what colors represent a strong intensity? What does that tell you about the gene expression level of the gene the feature represents? In a colors display image, what gene expression level does each color indicate? ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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