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pGLO
pGLO

... In this lab, you will perform a procedure known as genetic transformation. Genetic transformation occurs when a cell takes up and expresses a new piece of genetic material (DNA). This new genetic information often provides the organism with a new trait which is identifiable after transformation is c ...
Learning Standards for Biology Cells I can identify cell organelles
Learning Standards for Biology Cells I can identify cell organelles

Genome-scale CRISPR pooled screens
Genome-scale CRISPR pooled screens

... sequence in the genome, it creates a double-strand break (DSB). DSB repair mechanisms, such as non-homologous end-joining (NHEJ), can delete or add a few bases during the repair process. When the NHEJ-mediated repair occurs in a coding region, this can introduce a frameshift mutation where the net r ...
DNA-Mediated Transformation
DNA-Mediated Transformation

... Changes in bacterial traits Caused by: • Changes in environmental conditions (only phenotypic changes) • Changes in the genetic codes 1- Intermicrobial exchange 2- Mutations (point mutations, insertions, deletions) ...
Faith and the Human Genome
Faith and the Human Genome

... about 30,000 for the number of human genes. Considering that we’ve been talking about 100,000 genes for the last fifteen years (that’s what most of the textbooks still say), this was a bit of a shock. In fact, some people took it quite personally. I think they were particularly distressed because th ...
Cherry self-incompatibility
Cherry self-incompatibility

... Jewels in the genome By Amy Iezzoni, Project Director What is a “Jewel in the Genome?” An individual’s genome is the full complement of genetic information that it inherited from its parents. Within this vast repertoire of genetic information, individual genes are being discovered that control criti ...
ITMI2009_028
ITMI2009_028

... 61, 65 and 97 putative recombinants were selected in the families 2A6Nv, 2B-6Nv and 2D-6Nv before the meiosis stage. Anthers at the MI stage of meiosis were collected on each plant. Meiotic analysis revealed that most selected plants were double monosomics or addition plants. Only two plants in the ...
MECHANISMS OF GENETIC CHANGE
MECHANISMS OF GENETIC CHANGE

... to the bcr gene. •This makes a fusion of two genes that would not normally be together. It is called the bcr-abl fusion gene and it sits on ...
Prof. Kamakaka`s Lecture 14 Notes
Prof. Kamakaka`s Lecture 14 Notes

Activity--Extracting DNA - e
Activity--Extracting DNA - e

... traits also produces and controls the traits of other living things, although the amount and the coding are different. Today, scientists analyze the DNA from minute samples of blood, hair, saliva, and other body fluids. They use the analyses for many different scientific studies. Forensic studies us ...
C:\BOB\HSC\Exams 05\Supps\Biology 3201 August 2005.wpd
C:\BOB\HSC\Exams 05\Supps\Biology 3201 August 2005.wpd

... Which is most likely the ratio resulting from a monohybrid cross with codominance if both parents are hybrid? (A) (B) (C) (D) ...
Ch 21 47 Notes - Dublin City Schools
Ch 21 47 Notes - Dublin City Schools

Document
Document

... encode the twenty standard amino acids, giving most amino acids more than one possible codon. There are also three 'stop' or 'nonsense' codons signifying the end of the coding region; these are the TAA, TGA and TAG codons. ...
minireview - International Journal of Systematic and Evolutionary
minireview - International Journal of Systematic and Evolutionary

... novel promoter, 7 of the inversions occurred in the intercistronic sequence between the hisD and hisG loci (25). Furthermore, relatively short cruciform structures have been identified as being formally equivalent to sites at which crossing over is postulated to occur (12). A definitive interpretati ...
minireview - International Journal of Systematic and Evolutionary
minireview - International Journal of Systematic and Evolutionary

... novel promoter, 7 of the inversions occurred in the intercistronic sequence between the hisD and hisG loci (25). Furthermore, relatively short cruciform structures have been identified as being formally equivalent to sites at which crossing over is postulated to occur (12). A definitive interpretati ...
Notes - marric.us
Notes - marric.us

... 17. Which is the most highly mutagenic? 18. Look at the following figure. Identify the proteins that DNA first coils around. 19. Explain how Hox genes affect an organism. ...
Annotation of Drosophila virilis
Annotation of Drosophila virilis

... Enter coordinates into gene model checker to confirm it is a valid model 2. Use custom tracks (magnifying glass) to view model and double check that the final model agrees with all your evidence 3. Examine dot plot to discover possible ...
Text S6
Text S6

... production of xenocoumacins, xenematide, xenortides have all been identified in the genome of X. nematophila and the biosynthesis genes for the production of the indole derivatives in X. bovienii (Bode, unpublished): No biosynthesis gene cluster could be identified for the production of nematophin, ...
Comparative mycobacterial genomics Stewart T Cole
Comparative mycobacterial genomics Stewart T Cole

... devoted to genes encoding two different classes of proteins: enzymes involved in fatty acid metabolism and acidic, glycine-rich polypeptides of unknown function, the PE and PPE proteins [1••,11]. The mycobacterial cell envelope contains a dazzling array of lipids, glycolipids, mycolic acids and poly ...
Collect, analyze and synthesize
Collect, analyze and synthesize

... However when amino acid conservation is absent, other evidence must be considered. See the handout “Annotation Instruction Sheet” for more help. ...
современные проблемы молекулярной биологии
современные проблемы молекулярной биологии

... A Promoter, CAP, leader, Coding region, stop codon, trailer, poly(A) tail B CAP, Promoter, leader, Coding region, stop codon, trailer, poly(A) tail C Promoter, CAP, leader, Coding region, stop codon, poly(A) tail, trailer, D Promoter, leader, CAP, Coding region, stop codon, trailer, poly(A) tail E P ...
Document
Document

... BLASTs to be performed and to speed the process, we downloaded the text or “flat file” of the TIGR rice protein sequences (available at: http://www.tigr.org/tdb/e2k1/osa1/data_download.shtml) and performed local blasts using blastall from NCBI (available at: http://www.ncbi.nlm.nih.gov/BLAST/downloa ...
Bio 211 Genetics Laboratory Experiment 5: Bioinformatics
Bio 211 Genetics Laboratory Experiment 5: Bioinformatics

... Bioinformatics is the field and study of biological information in DNA using computer‐ based approaches.  Through program algorithms, coding sequences, promoters, and other  functional DNA sequences can be identified from databases of genomic information, and  interspecific comparisons can be made t ...
Lecture 7 - School of Science and Technology
Lecture 7 - School of Science and Technology

... • As size of genomes varies dramatically from 10,000 bp for simple viruses up to several billion bp in higher animals and plants, the number of sequences covering the whole genome also varies very significantly 10 – 106. • DNA fragments presented in DB have not only very different lengths but also d ...
UNIT 7
UNIT 7

... between two homologues (sister chromatid exchange). The site of crossing over is called a chiasma (Figure 8.18A). B. This happens between chromatids within tetrads, as homologues pair up during synapsis (prophase I). C. Crossing over produces new combinations of genes (genetic recombination) (Figure ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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