• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Exercises
Exercises

LacI_Biochem.ppt
LacI_Biochem.ppt

... Binding of regulators to DNA is often at inverted repeats Binding sites have inverted repeats Regulator proteins bind as back-to-back dimer. This puts each binding domain in the right orientation with respect to the inverted repeat ...
Ecological Risks of Gene Drive Technologies
Ecological Risks of Gene Drive Technologies

... CRISPR (Clustered regularly-interspaced short palindromic repeats) are segments of DNA from bacterial immune systems. An accompanying protein, such as Cas9 (CRISPR-associated protein 9), acts as molecular scissors that cut DNA at a target sequence specified by a small guide molecule (RNA). This syst ...
Genetic Analysis of RpL38 and RpL5, Two Minute Genes Located in
Genetic Analysis of RpL38 and RpL5, Two Minute Genes Located in

... unpublished results). This relatively poor Minute-to-RP gene correspondence is because most classical Minutes ...
slides
slides

... • Is this mechanism used to control the access to specific binding sites? ...
Chromosomal Microarray (CGH+SNP)
Chromosomal Microarray (CGH+SNP)

... chromosomal imbalances, and will detect aneuploidy, deletions, and duplications of the loci represented on the array. This analysis will also detect regions of uniparental isodisomy as well as regions of homozygosity (ROH). The possible clinical significance of ROH relates to two different classes o ...
genetics chapter - UBC Let`s Talk Science
genetics chapter - UBC Let`s Talk Science

... wondered what exactly that means? Or why you have the same bright red hair as your dad? Or why all of your uncles are bald? A lot of questions like these can be answered by genetics. Genetics refers to the study of genes, or DNA. DNA stands for deoxyribonucleic acid and it acts as a sort of instruct ...
File - Molecular Biology 2
File - Molecular Biology 2

... in the genome. Although most of the DNA in mammalian genomes does not consist of genes, still, isolating any one gene is like searching for the proverbial needle in a haystack. Most techniques used in the analysis of genes and other DNA sequences require that the sequence be available in significant ...
Chapter 10 - McGraw Hill Higher Education
Chapter 10 - McGraw Hill Higher Education

... The sequence of the entire human genome was reported on June 26, 2000 It consists of 3.2 billion base pairs If the human genome were a book It would be 500,000 pages long It would take about 60 years to read at the rate of 8 hours a day, every day, at five bases a second ...
Genetic Linkage Analysis
Genetic Linkage Analysis

Site-Specific Integration of Transgenes in
Site-Specific Integration of Transgenes in

... recombinase-mediated site-specific integration (SSI) are promising technologies to address the challenges for placing a single copy of transgenes into a precharacterized site in a plant genome. Several site-specific DNA recombination systems, such as the bacteriophage Cre-lox and the yeast FLPFRT an ...
"Engineering Large Animal Species to Model Human Diseases". In
"Engineering Large Animal Species to Model Human Diseases". In

... repaired by the error-prone nonhomologous end joining (NHEJ) process. This process results in variable sized insertions or deletions (indels), which can yield frameshift mutations, effectively knocking out the targeted gene. Alternatively, if the nuclease is provided with a homology-directed repair ...
Genetic Studies of Recombining DNA in
Genetic Studies of Recombining DNA in

... at a value of approximately 1/64. Clearly, the LE gene was being destroyed inside the cell to an extent equivalent to its probability of genetic integration. It was suggested at that time that following penetration into the cell, the LE marker was rapidly destroyed, probably by the recombination pro ...
Rice Bioinformatics. Analysis of Rice Sequence Data and
Rice Bioinformatics. Analysis of Rice Sequence Data and

... clone, and sequence data for Arabidopsis biologists. Likewise, with the generation of a vast amount of rice genomic sequence data, the necessity to integrate rice sequence data with other information from rice genetics, breeding, physiology, and biochemistry is apparent. Several centers have develop ...
Identification of a 5S rDNA spacer type specific to Triticum urartu and
Identification of a 5S rDNA spacer type specific to Triticum urartu and

improvement of the individual and improvement of the human species
improvement of the individual and improvement of the human species

... PGH has resulted in sophisticated techniques for the diagnosis of genetic fetal afflictions during pregnancy (prenatal diagnostics) [18] . This has had a particular development in countries where abortion is permitted. In those countries, when a fetus is found to have a genetic affliction, such as D ...
Current Microbiology
Current Microbiology

... indole-3-pyruvate decarboxylase of Enterobacter cloacae [7], and 95.1% identical (96.6% similar) amino acid residues with the sequence of the indole-3-pyruvate decarboxylase of A. brasilense Sp 245 [5] was detected, the ORF1 was named ipdC. However, upstream of position -26 referred to the ATG start ...
Better Living Through Genetics
Better Living Through Genetics

... Making Sense of the Research Bacterial and viral infections can be hard to spot. Often, a diagnosis is made based on symptoms. In the case of viral infections, even a firm diagnosis is done indirectly, by looking for antibodies that the body makes to fight the virus. This technique may make it poss ...
Lab 1 Meta
Lab 1 Meta

... scientists are starting to gain a deeper understanding of what contributes to flower coloration and how it can be manipulated. This study sought to explore the mechanism behind the variegated flower coloration of the Dahlia cultivar Dixie’s Winedot. Dixie’s Winedot flowers have a pale yellow backgro ...
Phylogenetic relationships among iguanian lizards using alternative
Phylogenetic relationships among iguanian lizards using alternative

... TVM + C + I as the optimal model with the Akaike Information Criterion. However, current implementation of RAxML does not allow for specification of the TVM substitution model. Proportion of invariant sites was not estimated so that ML results were comparable to the model assumed in Bayesian analyses ...
Globin gene family
Globin gene family

... • Genetic differences between closely related species can be correlated with phenotypic differences • For example, genetic comparison of several mammals with nonmammals helps identify what it takes to make a mammal ...
Extreme Evolution
Extreme Evolution

... Another distinctive adaptation that has emerged independently multiple times is enlarged lips in species that target prey found in rock crevices. My colleagues and I have shown that these “Angelina Jolie” lips act as seals and bumpers that help the fishes suck prey out from their hiding spots. (Cich ...
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences

... CadA, a Cd(II)-translocating P-type ATPase, is encoded by the cadA gene of plasmid pI258 from Staphylococcus aureus (14). The closest homolog to CadA is the putative product of o732 (Fig. 1A), one of several potential soft metaltranslocating P-type ATPases identified in the E. coli genome (21, 22). ...
Lecture 8
Lecture 8

... • DNA chips (microarrays, gene chips) can be used for global analysis of gene expression during biological responses. • Arrays of different DNA oligonucleotides anchored to a glass or nylon substrate in a grid. • ~1 million oligonucleuotides can by simultaneously synthesized using photolithography a ...
Draft Declaration Robert Nussbaum1 18 10[1]
Draft Declaration Robert Nussbaum1 18 10[1]

... the rest of the DNA relies on the sequence. Although separation may be accomplished by biochemical methods, such as excising that segment or amplifying it by PCR, it is also possible to use biological methods to separate the DNA containing a gene away from other genes without extracting it. Random p ...
< 1 ... 96 97 98 99 100 101 102 103 104 ... 445 >

Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report