A Bacterial Plasmid: What can you tell me about the plamid?
... organism’s DNA. Create sticky ends that are complementary to the plasmid’s sticky ends. • Insert the gene using ligase. How does one determine which RE’s to use? ...
... organism’s DNA. Create sticky ends that are complementary to the plasmid’s sticky ends. • Insert the gene using ligase. How does one determine which RE’s to use? ...
Mathematical Tools for Understanding Genome Rearrangements
... Wednesday September 19, 2012 12:10 - 12:50 p.m. *CETL (OL 1142)* The diversity of life is a direct result of inaccuracy in DNA replication. At some point in the past, humans and mice had a common ancestor, and many "mistakes" later, we have two apparently very different species. At the level of DNA, ...
... Wednesday September 19, 2012 12:10 - 12:50 p.m. *CETL (OL 1142)* The diversity of life is a direct result of inaccuracy in DNA replication. At some point in the past, humans and mice had a common ancestor, and many "mistakes" later, we have two apparently very different species. At the level of DNA, ...
notes
... Bacteria provide the means • Bacteria have been vital in developing DNA technology • Thermus aquaticus (which lives in hot springs) provides DNA polymerase enzyme for PCR • Escherichia coli (which lives in our guts) provides “plasmids” (mini-chromosomes) used in cloning • 100s of bacterial species ...
... Bacteria provide the means • Bacteria have been vital in developing DNA technology • Thermus aquaticus (which lives in hot springs) provides DNA polymerase enzyme for PCR • Escherichia coli (which lives in our guts) provides “plasmids” (mini-chromosomes) used in cloning • 100s of bacterial species ...
Genome Editing Slides
... • Discovered as what prokaryotes have as an immune system • Pallindromic Repeats of 20-40 bases, separated by short sequences that turn out to be leftover from bacterial viruses that had previously infected the cell – Pallindromic DNA, when transcribed make RNA’s that can base pair with themselves t ...
... • Discovered as what prokaryotes have as an immune system • Pallindromic Repeats of 20-40 bases, separated by short sequences that turn out to be leftover from bacterial viruses that had previously infected the cell – Pallindromic DNA, when transcribed make RNA’s that can base pair with themselves t ...
SW describe how techniques such as DNA
... Sex-influenced traits are those that are expressed differently in the two sexes. Such traits are autosomal, which means that the genes responsible for their expression are not carried on the sex chromosomes. ...
... Sex-influenced traits are those that are expressed differently in the two sexes. Such traits are autosomal, which means that the genes responsible for their expression are not carried on the sex chromosomes. ...
Lab Quiz 4 Key
... 7. Why is arabinose added to one of the petri dishes in the bacterial transformation lab? (0.5 pt) [the GFP gene will only get switched on as part of a metabolic sequence that processes the sugar arabinose. In order for the metabolic pathway to be switched on, arabinose must be present (acts ...
... 7. Why is arabinose added to one of the petri dishes in the bacterial transformation lab? (0.5 pt) [the GFP gene will only get switched on as part of a metabolic sequence that processes the sugar arabinose. In order for the metabolic pathway to be switched on, arabinose must be present (acts ...
Re-closing linearized plasmids
... method. Make sure to pre-grow the cells in SOC or LB medium with no antibiotic prior to plating. See the protocol page for “Transformation of E. coli.” Screening for correct clones: • Pick 3-6 single, isolated colonies and inoculate each into a separate 14-mL tube containing 5 mL of liquid LB with t ...
... method. Make sure to pre-grow the cells in SOC or LB medium with no antibiotic prior to plating. See the protocol page for “Transformation of E. coli.” Screening for correct clones: • Pick 3-6 single, isolated colonies and inoculate each into a separate 14-mL tube containing 5 mL of liquid LB with t ...
Microorganisms in Biotechnology
... Recombinant DNA Technology • In the medical field, cultured cells are being used to manufacture products such as: ...
... Recombinant DNA Technology • In the medical field, cultured cells are being used to manufacture products such as: ...
Microbial Genetics
... • Analogs for bases – 5-bromo-uracil for thymine (5BU can pair with G as well as with A) – 2-aminopurine for adenine (2AP can pair with C as well as with T) ...
... • Analogs for bases – 5-bromo-uracil for thymine (5BU can pair with G as well as with A) – 2-aminopurine for adenine (2AP can pair with C as well as with T) ...
Apple Molecular Biology: Animation 2
... 5. Then complete the review questions on this worksheet using what you learned from the reading and animation. Cloning and Replication A plasmid is a small circular strand of chromosome, and is found in bacteria. Generally, they include some region of DNA that confers antibiotic resistance so any or ...
... 5. Then complete the review questions on this worksheet using what you learned from the reading and animation. Cloning and Replication A plasmid is a small circular strand of chromosome, and is found in bacteria. Generally, they include some region of DNA that confers antibiotic resistance so any or ...
The Human Genome Project CH 13 Sec 3 notes
... •Place in microarray slide –incubate •Examine colors for gene expression –Yellow = same in both cells –red =higher in cancer cells –green = higher in normal cells Black = no expression Genetics Disorders in the genome •_______ of nucleotides are the same in all people •Variation that occurs in a gen ...
... •Place in microarray slide –incubate •Examine colors for gene expression –Yellow = same in both cells –red =higher in cancer cells –green = higher in normal cells Black = no expression Genetics Disorders in the genome •_______ of nucleotides are the same in all people •Variation that occurs in a gen ...
Imaging in CRISPR/Cas9 Applications
... The CRISPR/Cas9 system is an exciting methodology for genetic modification. Aubrey, Kelly et al. have advanced this technology by developing an inducible lentiviral system. This platform facilitates efficient gene targeting and utilizes an imagingbased tool for phenotypic assessment following deleti ...
... The CRISPR/Cas9 system is an exciting methodology for genetic modification. Aubrey, Kelly et al. have advanced this technology by developing an inducible lentiviral system. This platform facilitates efficient gene targeting and utilizes an imagingbased tool for phenotypic assessment following deleti ...
a10c Biotechnology
... 2. What is a restriction enzyme, and what does it catalyze? How do restriction enzymes differ in what they cleave? What do they "look for"? Name an example of a restriction enzyme. 3. Describe the steps of cloning (transferring a gene to bacteria for purposes of "growing" DNA or protein). What enzym ...
... 2. What is a restriction enzyme, and what does it catalyze? How do restriction enzymes differ in what they cleave? What do they "look for"? Name an example of a restriction enzyme. 3. Describe the steps of cloning (transferring a gene to bacteria for purposes of "growing" DNA or protein). What enzym ...
DNA and Chromosomes
... What is the relationship between DNA, chromosomes, and any organism? Drag and drop the descriptive phrase to the correct column, thereby helping us to describe the relationships between these important components of inheritance. ...
... What is the relationship between DNA, chromosomes, and any organism? Drag and drop the descriptive phrase to the correct column, thereby helping us to describe the relationships between these important components of inheritance. ...
GE Nova Video Questions
... Note: This video is 15 minutes in total. The answers to the worksheet are found between 6.55 minutes and 10 minutes approx. ...
... Note: This video is 15 minutes in total. The answers to the worksheet are found between 6.55 minutes and 10 minutes approx. ...
Document
... Cut DNA molecules from various organisms and recombine pieces Recombinant DNA Restrict the growth of viruses in bacteria Digest the DNA molecule at specific nucleotide sequences ...
... Cut DNA molecules from various organisms and recombine pieces Recombinant DNA Restrict the growth of viruses in bacteria Digest the DNA molecule at specific nucleotide sequences ...
Review Questions yeast lecture 18
... copies due to the 2 micron origin of replication. YCp plasmids are present in low copy number (usually one) due to the centromeric fragment. 6. What is a YAC? (1) ...
... copies due to the 2 micron origin of replication. YCp plasmids are present in low copy number (usually one) due to the centromeric fragment. 6. What is a YAC? (1) ...