DNA Sequencing by Targeting 16S rRNA Gene for Novel Strain
... stable in a wide range of harsh environments. Previous researches had been done to identify the enzymes producing bacteria based upon the traditional methods. There are two major drawbacks of traditional methods of bacterial identification. First, they can be used only for organisms that can be cult ...
... stable in a wide range of harsh environments. Previous researches had been done to identify the enzymes producing bacteria based upon the traditional methods. There are two major drawbacks of traditional methods of bacterial identification. First, they can be used only for organisms that can be cult ...
Preimplantation genetic diagnosis: State of the ART 2011
... for fewer manipulations as both polar bodies are removed at the same time, but it may be difficult to distinguish between the first and second polar body (which might be necessary for some diagnoses) and the first polar body may degenerate. Sequential biopsy requires two biopsy stages but has the ad ...
... for fewer manipulations as both polar bodies are removed at the same time, but it may be difficult to distinguish between the first and second polar body (which might be necessary for some diagnoses) and the first polar body may degenerate. Sequential biopsy requires two biopsy stages but has the ad ...
Detection of the Most Common Genetic Causes of
... 1A Y isoform gene (EIF1AY) and the RNA binding motif (RBM) family are found on AZFb region. EIF1AY encodes an essential translation factor. The PTP-BL-related Y (PRY) family of genes is mapped to AZFb and AZFc regions and encodes proteins proposed to be involved in apoptosis. RBM and deleted-in-azoo ...
... 1A Y isoform gene (EIF1AY) and the RNA binding motif (RBM) family are found on AZFb region. EIF1AY encodes an essential translation factor. The PTP-BL-related Y (PRY) family of genes is mapped to AZFb and AZFc regions and encodes proteins proposed to be involved in apoptosis. RBM and deleted-in-azoo ...
Submitting an RNA-Seq job at PATRIC
... 4. Transcripts.txt file. This text file contains the data on a per gene bases. This data includes the contig, the transcription and translation stop and end sites, the strand, PATRIC and RefSeq locus tags, the functional description, estimates of abundance levels per gene, and the q-value. Est ...
... 4. Transcripts.txt file. This text file contains the data on a per gene bases. This data includes the contig, the transcription and translation stop and end sites, the strand, PATRIC and RefSeq locus tags, the functional description, estimates of abundance levels per gene, and the q-value. Est ...
Review Molecular Biology in Arteriosclerosis Research
... The topics discussed In this article illustrate how molecular biology will have a dramatic Impact on arteriosclerosis research. DNA clones for a small number of relevant proteins have been isolated, and studies are underway in numerous laboratories to extend these initial studies. The techniques of ...
... The topics discussed In this article illustrate how molecular biology will have a dramatic Impact on arteriosclerosis research. DNA clones for a small number of relevant proteins have been isolated, and studies are underway in numerous laboratories to extend these initial studies. The techniques of ...
- SciTech Connect
... RNA there, and the risk of contamination from other cells. Next-generation sequencing techniques as well as advances in cell sorting have been instrumental in the analysis of sperm RNA populations. Studies in several species have confirmed the presence, not only of mRNAs, but also of a variety of no ...
... RNA there, and the risk of contamination from other cells. Next-generation sequencing techniques as well as advances in cell sorting have been instrumental in the analysis of sperm RNA populations. Studies in several species have confirmed the presence, not only of mRNAs, but also of a variety of no ...
CRISPR-Cas9 Mouse Toolbox
... they designed an sgRNA targeting LacZ, which is not present in the mouse genome. These plasmids are described here. 4. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and an sgRNA targeted to the mouse NeuN gene. ...
... they designed an sgRNA targeting LacZ, which is not present in the mouse genome. These plasmids are described here. 4. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and an sgRNA targeted to the mouse NeuN gene. ...
Advances in maize genomics: the emergence of positional cloning
... magnitude as that required in Arabidopsis [24], even though the maize genome is about 20 times larger than that of Arabidopsis. In retrospect, this finding is not too surprising, as recombination occurs mostly within genes [25], and the gene numbers in Arabidopsis and maize might not be too differen ...
... magnitude as that required in Arabidopsis [24], even though the maize genome is about 20 times larger than that of Arabidopsis. In retrospect, this finding is not too surprising, as recombination occurs mostly within genes [25], and the gene numbers in Arabidopsis and maize might not be too differen ...
Answer Appendix B - McGraw Hill Higher Education
... C16. First construct a Punnett square. The chances are 75% of producing a solid pup and 25% of producing a spotted pup. A. Use the binomial expansion equation, where n = 5, x = 4, p = 0.75, q = 0.25. The answer is 0.396 = 39.6% of the time. B. You can use the binomial expansion equation for each lit ...
... C16. First construct a Punnett square. The chances are 75% of producing a solid pup and 25% of producing a spotted pup. A. Use the binomial expansion equation, where n = 5, x = 4, p = 0.75, q = 0.25. The answer is 0.396 = 39.6% of the time. B. You can use the binomial expansion equation for each lit ...
Molecular Characterization of a Chromosomal Rearrangement Involved in the Adaptive Evolution
... alpechin (olive residues after oil extraction), respectively, in Spain, a wine-producing country, and 10691, isolated from palm wine in West Africa. In contrast, variants C and D are present in laboratory strains, but also in strains 1462, 11837, and 11838 isolated from ale beer, bili wine and grape ...
... alpechin (olive residues after oil extraction), respectively, in Spain, a wine-producing country, and 10691, isolated from palm wine in West Africa. In contrast, variants C and D are present in laboratory strains, but also in strains 1462, 11837, and 11838 isolated from ale beer, bili wine and grape ...
Genomic Organization of Evolutionarily Correlated Genes in
... benchmark data set consisting of 2254 proteincoding genes contributing to 22,500 gene pairs. 7 These pairs had been identified by comparing 105 bacterial genomes on the basis of two types of evolutionary correlations: 7 a tendency to be located close together in many genomes, independently of their r ...
... benchmark data set consisting of 2254 proteincoding genes contributing to 22,500 gene pairs. 7 These pairs had been identified by comparing 105 bacterial genomes on the basis of two types of evolutionary correlations: 7 a tendency to be located close together in many genomes, independently of their r ...
The complete inventory of the yeast Saccharomyces cerevisiae P
... on both sides by cysteine residues (Table 2). The SEHPL motif with invariant H and P located about 40 residues downstream the phosphorylation site was reported to be also characteristic of Pl-ATPases [5,6]. It is represented as SDHPV and IKHPV in the yeast Ydr270wp and Ybr295wp respectively. Both ye ...
... on both sides by cysteine residues (Table 2). The SEHPL motif with invariant H and P located about 40 residues downstream the phosphorylation site was reported to be also characteristic of Pl-ATPases [5,6]. It is represented as SDHPV and IKHPV in the yeast Ydr270wp and Ybr295wp respectively. Both ye ...
Requirements for Human Medical Genome
... Medical testing by genomic methods shares many ethical, legal and social issues with other forms of clinical investigation. Existing issues of informed consent, incidental findings, the right not to know, family studies and re-contacting are potentially magnified due to the volume of information tha ...
... Medical testing by genomic methods shares many ethical, legal and social issues with other forms of clinical investigation. Existing issues of informed consent, incidental findings, the right not to know, family studies and re-contacting are potentially magnified due to the volume of information tha ...
Variations on a theme: Genomics of sex
... avian W; in contrast to this, sex chromosomes in fish, if present at all, are often homomorphic and differentiation is not detectable by karyotyping [2]. Overall, fish show frequent turnover of sex-determining systems [5], which stands in sharp contrast to other vertebrate clades, especially to bird ...
... avian W; in contrast to this, sex chromosomes in fish, if present at all, are often homomorphic and differentiation is not detectable by karyotyping [2]. Overall, fish show frequent turnover of sex-determining systems [5], which stands in sharp contrast to other vertebrate clades, especially to bird ...
Distortion of quantitative genomic and expression
... increases of 1.08- and 1.14-fold in hybridization intensity in the presence of Cot-1 DNA (Table 1, reactions 3–6). These experiments suggested that the effects due to Cot-1 are related to the composition of repetitive sequences surrounding these sc intervals. An sc probe from HOXB1 (chr 17: 43 964 2 ...
... increases of 1.08- and 1.14-fold in hybridization intensity in the presence of Cot-1 DNA (Table 1, reactions 3–6). These experiments suggested that the effects due to Cot-1 are related to the composition of repetitive sequences surrounding these sc intervals. An sc probe from HOXB1 (chr 17: 43 964 2 ...
Cytogenetics Cytogenetics
... another chromosome, or rarely within the same chromosome. • Recorded as ins, followed by a bracket with the number of the chromosome which receives the segment preceding the number of the chromosome which donates the segment eg ins(2)(p13q31q34) and ins(5;2)(p12;q31q34): the segment q31q34 from a ch ...
... another chromosome, or rarely within the same chromosome. • Recorded as ins, followed by a bracket with the number of the chromosome which receives the segment preceding the number of the chromosome which donates the segment eg ins(2)(p13q31q34) and ins(5;2)(p12;q31q34): the segment q31q34 from a ch ...
Caspary T, Cleary MA, Baker CC, Guan XJ, Tilghman SM. Mol Cell Biol. 1998 Jun;18(6):3466-74. Multiple mechanisms of imprinting on distal mouse chromosome 7.
... the tendency of imprinted genes to exist in chromosomal clusters suggests long-range regulation through shared regulatory elements. We characterize a 800-kb region on the distal end of mouse chromosome 7 that contains a cluster of four maternally expressed genes, H19, Mash2, Kvlqt1, and p57Kip2, as ...
... the tendency of imprinted genes to exist in chromosomal clusters suggests long-range regulation through shared regulatory elements. We characterize a 800-kb region on the distal end of mouse chromosome 7 that contains a cluster of four maternally expressed genes, H19, Mash2, Kvlqt1, and p57Kip2, as ...
HMMs for gene predictions.
... • Parameters are empirically determined by examining a “large” set of example genes • This is not perfect – Genes sometimes have unusual codons for a reason – The predictive power is dependent on length of sequence ...
... • Parameters are empirically determined by examining a “large” set of example genes • This is not perfect – Genes sometimes have unusual codons for a reason – The predictive power is dependent on length of sequence ...
Phenotypic overlap in the contribution of individual genes to CNV
... randomized sets of genes. Our results are highly statistically significant; compared to the analysed 27 CNV diseases, a total of ~480 of their respective phenotypic features could be explained, whereas in the randomized experiments, the average number of phenotypic features explained was ~250 (Figur ...
... randomized sets of genes. Our results are highly statistically significant; compared to the analysed 27 CNV diseases, a total of ~480 of their respective phenotypic features could be explained, whereas in the randomized experiments, the average number of phenotypic features explained was ~250 (Figur ...
Functional genomics of plant photosynthesis in
... proteins into multisubunit complexes, and indeed the Nac2 protein was shown to be part of a high molecular weight complex associated with non-polysomal RNA. RNA binding by Nac2 has not been shown, therefore Nac2 might bind RNA via an additional unidentified subunit. Candidates for this role include ...
... proteins into multisubunit complexes, and indeed the Nac2 protein was shown to be part of a high molecular weight complex associated with non-polysomal RNA. RNA binding by Nac2 has not been shown, therefore Nac2 might bind RNA via an additional unidentified subunit. Candidates for this role include ...
SAB-2010
... Based on the SAB suggestions, the progress has been made towards the goal of extending the annotation of pathway databases in Cyc and Wiki versions in an automated way. However to do that approach we have to streamline the data workflow and structure the current curated gene database as a central re ...
... Based on the SAB suggestions, the progress has been made towards the goal of extending the annotation of pathway databases in Cyc and Wiki versions in an automated way. However to do that approach we have to streamline the data workflow and structure the current curated gene database as a central re ...
Genomic surveys and expression analysis of bZIP gene family in
... Abstract The basic leucine zipper (bZIP) transcription factors comprise a family of transcriptional regulators present extensively in plants, involved in regulating diverse biological processes such as flower and vascular development, seed maturation, stress signaling and pathogen defense. Castor be ...
... Abstract The basic leucine zipper (bZIP) transcription factors comprise a family of transcriptional regulators present extensively in plants, involved in regulating diverse biological processes such as flower and vascular development, seed maturation, stress signaling and pathogen defense. Castor be ...
Author`s personal copy - Real Jardín Botánico
... 1055-7903/$ - see front matter Ó 2011 Elsevier Inc. All rights reserved. doi:10.1016/j.ympev.2011.06.023 ...
... 1055-7903/$ - see front matter Ó 2011 Elsevier Inc. All rights reserved. doi:10.1016/j.ympev.2011.06.023 ...
Unearthing the Roles of Imprinted Genes in the Placenta
... The development of lineage-specific cells begins with the differentiation of the trophoblast lineage and the inner cell mass [16]. This event depends on epigenetic modifications that control the expression of particular genes, allowing cells to develop and differentiate into specific cells and tissues ...
... The development of lineage-specific cells begins with the differentiation of the trophoblast lineage and the inner cell mass [16]. This event depends on epigenetic modifications that control the expression of particular genes, allowing cells to develop and differentiate into specific cells and tissues ...
Human genome
The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. Human genomes include both protein-coding DNA genes and noncoding DNA. Haploid human genomes, which are contained in germ cells (the egg and sperm gamete cells created in the meiosis phase of sexual reproduction before fertilization creates a zygote) consist of three billion DNA base pairs, while diploid genomes (found in somatic cells) have twice the DNA content. While there are significant differences among the genomes of human individuals (on the order of 0.1%), these are considerably smaller than the differences between humans and their closest living relatives, the chimpanzees (approximately 4%) and bonobos. Humans share 50% of their DNA with bananas.The Human Genome Project produced the first complete sequences of individual human genomes, with the first draft sequence and initial analysis being published on February 12, 2001. The human genome was the first of all vertebrates to be completely sequenced. As of 2012, thousands of human genomes have been completely sequenced, and many more have been mapped at lower levels of resolution. The resulting data are used worldwide in biomedical science, anthropology, forensics and other branches of science. There is a widely held expectation that genomic studies will lead to advances in the diagnosis and treatment of diseases, and to new insights in many fields of biology, including human evolution.Although the sequence of the human genome has been (almost) completely determined by DNA sequencing, it is not yet fully understood. Most (though probably not all) genes have been identified by a combination of high throughput experimental and bioinformatics approaches, yet much work still needs to be done to further elucidate the biological functions of their protein and RNA products. Recent results suggest that most of the vast quantities of noncoding DNA within the genome have associated biochemical activities, including regulation of gene expression, organization of chromosome architecture, and signals controlling epigenetic inheritance.There are an estimated 20,000-25,000 human protein-coding genes. The estimate of the number of human genes has been repeatedly revised down from initial predictions of 100,000 or more as genome sequence quality and gene finding methods have improved, and could continue to drop further. Protein-coding sequences account for only a very small fraction of the genome (approximately 1.5%), and the rest is associated with non-coding RNA molecules, regulatory DNA sequences, LINEs, SINEs, introns, and sequences for which as yet no function has been elucidated.