Thermal unfolding of proteins at high pH range studied by UV
... therefore tyrosyl ionization is detected. In this paper we measured the degree of ionization of tyrosine residues and used it to follow protein unfolding. The methodology proposed is very simple and easy to perform making it very useful to evaluate protein denaturation. Provided the number and natur ...
... therefore tyrosyl ionization is detected. In this paper we measured the degree of ionization of tyrosine residues and used it to follow protein unfolding. The methodology proposed is very simple and easy to perform making it very useful to evaluate protein denaturation. Provided the number and natur ...
Supplemental Material
... sequences were inspected individually, and group-level classification was done by applying the E-value cut-off for the characterised groups across the kinomes of H. sapiens, C. elegans, D. melanogaster and S. cerevisiae (MIRANDA-SAAVEDRA and BARTON 2007). Sequence comparison and clustering were carr ...
... sequences were inspected individually, and group-level classification was done by applying the E-value cut-off for the characterised groups across the kinomes of H. sapiens, C. elegans, D. melanogaster and S. cerevisiae (MIRANDA-SAAVEDRA and BARTON 2007). Sequence comparison and clustering were carr ...
Untitled
... The major lipid components of cell membranes are phospholipids which comprise ~50% of animal cell membranes (by mass). Phospholipids are amphipathic molecules; this means that they have both hydrophobic and hydrophilic parts. Shown here is phosphatidylcholine (PC), the predominant phospholipid in c ...
... The major lipid components of cell membranes are phospholipids which comprise ~50% of animal cell membranes (by mass). Phospholipids are amphipathic molecules; this means that they have both hydrophobic and hydrophilic parts. Shown here is phosphatidylcholine (PC), the predominant phospholipid in c ...
The biological meaning of pairwise alignments
... • What is the biological question? Examples: • Which proteins of the database are similar to my protein sequence? • Which proteins of the database are similar to the conceptual translation of my DNA sequence? • Which nucleotide sequences in the database are similar to my nucleotide sequence? • Which ...
... • What is the biological question? Examples: • Which proteins of the database are similar to my protein sequence? • Which proteins of the database are similar to the conceptual translation of my DNA sequence? • Which nucleotide sequences in the database are similar to my nucleotide sequence? • Which ...
Workshop: Protein Structure Introduction Learning Objectives
... e. Figure C.5 below shows one additional type of bond that can stabilize the tertiary structure of a protein. This bond is called a disulfide bond (or disulfide bridge), and it involves the sufhydryl (-SH) R groups from one particular type of amino acid. A disulfide bond can form only under certain ...
... e. Figure C.5 below shows one additional type of bond that can stabilize the tertiary structure of a protein. This bond is called a disulfide bond (or disulfide bridge), and it involves the sufhydryl (-SH) R groups from one particular type of amino acid. A disulfide bond can form only under certain ...
Identification of the Missing Links in Prokaryotic Pentose Oxidation
... Received for publication, June 9, 2006, and in revised form, July 17, 2006 Published, JBC Papers in Press, July 17, 2006, DOI 10.1074/jbc.M605549200 ...
... Received for publication, June 9, 2006, and in revised form, July 17, 2006 Published, JBC Papers in Press, July 17, 2006, DOI 10.1074/jbc.M605549200 ...
Proteolytic Enzymes in Detergents: Evidence of Their
... also be carried out if desired. In this case, no proteolytic activity is observed in any case (data not shown). Effect of Temperature on the Proteolytic Activity. Another condition that is known to affect chemical reactions is temperature. Different groups of students can use the same granule extrac ...
... also be carried out if desired. In this case, no proteolytic activity is observed in any case (data not shown). Effect of Temperature on the Proteolytic Activity. Another condition that is known to affect chemical reactions is temperature. Different groups of students can use the same granule extrac ...
Engineering of diffraction-quality crystals of the NF-κB
... In the search for high-quality co-crystals, badly ordered, weakly diffracting crystals are frequently encountered. In this paper we describe how rational engineering of the protein and variation of the length of the DNA duplexes yielded diffraction-quality co-crystals of the N F - K B P52 homodimer ...
... In the search for high-quality co-crystals, badly ordered, weakly diffracting crystals are frequently encountered. In this paper we describe how rational engineering of the protein and variation of the length of the DNA duplexes yielded diffraction-quality co-crystals of the N F - K B P52 homodimer ...
energy - Bide Sport
... The main focus of our dedicated work is to optimize the athlete’s performance. By doing so we have developed acid free sport drinks, well-tolerated gels or high quality recovery products. In close cooperation with our internationally renowned athletes and sports food opinion leaders we test and deve ...
... The main focus of our dedicated work is to optimize the athlete’s performance. By doing so we have developed acid free sport drinks, well-tolerated gels or high quality recovery products. In close cooperation with our internationally renowned athletes and sports food opinion leaders we test and deve ...
Role of N-terminal protein formylation in central metabolic processes
... affected by the lack of formylation in Δfmt. Of note, Δfmt released more of the central metabolic intermediate pyruvate to the growth medium than the wild type in the stationary phase suggesting that the metabolism of pyruvate was perturbed in the absence of protein formylation. Pyruvate and acetyl ...
... affected by the lack of formylation in Δfmt. Of note, Δfmt released more of the central metabolic intermediate pyruvate to the growth medium than the wild type in the stationary phase suggesting that the metabolism of pyruvate was perturbed in the absence of protein formylation. Pyruvate and acetyl ...
Novel Food Information - Drought Tolerant Corn - MON 87460
... collected for expression in all the previously assessed tissues under wellwatered and waterlimited conditions. Results from this trial were presented on a freshweight (fwt) and dryweight basis (dwt). Protein expression levels, both the mean and range values, were shown to be comparable between t ...
... collected for expression in all the previously assessed tissues under wellwatered and waterlimited conditions. Results from this trial were presented on a freshweight (fwt) and dryweight basis (dwt). Protein expression levels, both the mean and range values, were shown to be comparable between t ...
Chapter Five - DORAS
... The purification of membrane proteins from total membrane fractions can be facilitated by the use of affinity tags and immobilised metal ion affinity chromatography (IMAC) techniques. In silico analysis was required to establish the predicted size, location and topology of the membrane proteins prio ...
... The purification of membrane proteins from total membrane fractions can be facilitated by the use of affinity tags and immobilised metal ion affinity chromatography (IMAC) techniques. In silico analysis was required to establish the predicted size, location and topology of the membrane proteins prio ...
Zika Virus Proteins - Peptides International
... Aedes mosquitoes, such as A. aepregnant women can cause abnormal brain gypti and A. albopictus. The Zika virus is redevelopment in their fetuses by mother-tolated to the dengue, yellow fever, Japanese child transmission, which may result in misencephalitis, and West Nile viruses. Much carriage or mi ...
... Aedes mosquitoes, such as A. aepregnant women can cause abnormal brain gypti and A. albopictus. The Zika virus is redevelopment in their fetuses by mother-tolated to the dengue, yellow fever, Japanese child transmission, which may result in misencephalitis, and West Nile viruses. Much carriage or mi ...
RMA1, an Arabidopsis thaliana Gene Whose cDNA Suppresses the
... To identify molecules that function in the plant secretory pathway, we screened for Arabidopsis thaliana cDNA clones that complement the temperature-sensitive (ts), secretion-deficient seclS mutation of yeast Saccharomyces cerevisiae. RMA1, one of the genes obtained in this screening, suppressed not ...
... To identify molecules that function in the plant secretory pathway, we screened for Arabidopsis thaliana cDNA clones that complement the temperature-sensitive (ts), secretion-deficient seclS mutation of yeast Saccharomyces cerevisiae. RMA1, one of the genes obtained in this screening, suppressed not ...
Functional Activityof Protein S Determined with
... activated Protein C, a hereditary deficiency of it has also been associated with recurrent thrombosis (8-10). Several specific methods for determining Protein S antigen have already been established (11-14). Two types of Protein S deficiency are recognized. In type I, the patient has little or no fr ...
... activated Protein C, a hereditary deficiency of it has also been associated with recurrent thrombosis (8-10). Several specific methods for determining Protein S antigen have already been established (11-14). Two types of Protein S deficiency are recognized. In type I, the patient has little or no fr ...
Recovery Nutrition
... Electrolyte replacement is also crucial. Athletes can replace these electrolytes by consuming fruits, vegetables, sports drinks, as well as other foods and beverages. Electrolytes help control the energy used for normal bodily functions, including muscle contractions. Replacing electrolytes is an ex ...
... Electrolyte replacement is also crucial. Athletes can replace these electrolytes by consuming fruits, vegetables, sports drinks, as well as other foods and beverages. Electrolytes help control the energy used for normal bodily functions, including muscle contractions. Replacing electrolytes is an ex ...
CYP74C3 and CYP74A1, plant cytochrome P450 enzymes whose
... protein remained as a monomer of molecular mass 55 kDa [21], which suggested that there was no association with detergent micelles and that the protein was entirely watersoluble. In the same work, however, it was reported that the specific activity of the enzyme was enhanced 2–3-fold by detergent, b ...
... protein remained as a monomer of molecular mass 55 kDa [21], which suggested that there was no association with detergent micelles and that the protein was entirely watersoluble. In the same work, however, it was reported that the specific activity of the enzyme was enhanced 2–3-fold by detergent, b ...
Protein and Amino Acid Supplements
... • The protein requirement is based on several parameter(s) for e.g., whole-body protein mass, muscle mass, physical function, immune function, or metabolic function. Additionally, the protein requirement depends on quality—that is AA composition and digestibility—and physical activity level. • It is ...
... • The protein requirement is based on several parameter(s) for e.g., whole-body protein mass, muscle mass, physical function, immune function, or metabolic function. Additionally, the protein requirement depends on quality—that is AA composition and digestibility—and physical activity level. • It is ...
Comparative Visualization of Protein Structure
... such diverse tasks as facilitating chemical reactions and transporting molecules. It is the protein’s central role in the workings of the cell that makes it an important target for investigation. By studying protein structure, we gain insight into how proteins function, and how their properties can ...
... such diverse tasks as facilitating chemical reactions and transporting molecules. It is the protein’s central role in the workings of the cell that makes it an important target for investigation. By studying protein structure, we gain insight into how proteins function, and how their properties can ...
PACS-2 (Q-20): sc-160645 - Santa Cruz Biotechnology
... recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Wester ...
... recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Wester ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.