Identification of Both Shared and Distinct Proteins in the Major and
... Protein immunoblot analysis and sequence information from six of the eight other U11/U12-specific proteins failed to identify any additional U1 or U2 snRNP proteins or intron-bridging proteins such as SF1/ mBBP or FBP21 (19, 20). Instead, several previously uncharacterized polypeptides were detected ...
... Protein immunoblot analysis and sequence information from six of the eight other U11/U12-specific proteins failed to identify any additional U1 or U2 snRNP proteins or intron-bridging proteins such as SF1/ mBBP or FBP21 (19, 20). Instead, several previously uncharacterized polypeptides were detected ...
Inhibiting Biofilm Formation of Pseudomonas Aeruginosa
... analogues from those surface polysaccharides, this enzyme would indicate a novel therapeutic target for preventing bacterial infection of P. aeruginosa. Natural plant products provide a diverse array of chemical structures and are known to possess a plethora of biological activities. Pseudomonas aer ...
... analogues from those surface polysaccharides, this enzyme would indicate a novel therapeutic target for preventing bacterial infection of P. aeruginosa. Natural plant products provide a diverse array of chemical structures and are known to possess a plethora of biological activities. Pseudomonas aer ...
Interaction of cycloheximide with 25S ribosomal RNA from yeast
... that cycloheximide is exceptional in this respect. It is reasonable to predict that the drug binds to a site on 25s ribosomal RNA that is associated, in ribosomes, with ribosomal protein L41. This possibility is explored in the present communication. Ribosomes were prepared from S.cerevisiae and wer ...
... that cycloheximide is exceptional in this respect. It is reasonable to predict that the drug binds to a site on 25s ribosomal RNA that is associated, in ribosomes, with ribosomal protein L41. This possibility is explored in the present communication. Ribosomes were prepared from S.cerevisiae and wer ...
SeeProtStructFromPDB
... This tutorial serves as a basic introduction to viewing protein structures in Protein Data Bank (PDB), an international archive of structural data for biological molecules. In addition to summary information for a particular molecular structure, PDB also provides different viewing options. A structu ...
... This tutorial serves as a basic introduction to viewing protein structures in Protein Data Bank (PDB), an international archive of structural data for biological molecules. In addition to summary information for a particular molecular structure, PDB also provides different viewing options. A structu ...
Creating Multiple Sequence Alignments
... construct phylogenetic trees, to understand and interpret relationships between species. Activities: A. Creating Multiple Sequence Alignments (MSA) In this example, we will create a multiple alignment of protein sequences that will be imported into the alignment editor using different methods. Multi ...
... construct phylogenetic trees, to understand and interpret relationships between species. Activities: A. Creating Multiple Sequence Alignments (MSA) In this example, we will create a multiple alignment of protein sequences that will be imported into the alignment editor using different methods. Multi ...
A Guide to Finding - University of Utah
... The process of collecting protein information on the Internet starts at two “top-level” knowledgebases: NCBI and Expasy. Both provide five main types of information: sequence, domains, 3D structure, cross-reference, and descriptive (text). Each of these categories is described in more detail in the ...
... The process of collecting protein information on the Internet starts at two “top-level” knowledgebases: NCBI and Expasy. Both provide five main types of information: sequence, domains, 3D structure, cross-reference, and descriptive (text). Each of these categories is described in more detail in the ...
Protein Detection Methods in Proteomics Research
... destained with 25% (v/v) methanol, 65% (v/v) water and 10% acetic acid. Sometimes methanol was replaced by ethanol or isopropanol. Practice has shown that this procedure is not reproducible and is not very reliable for quantification. Because of the presence of alcohol some of the proteins release th ...
... destained with 25% (v/v) methanol, 65% (v/v) water and 10% acetic acid. Sometimes methanol was replaced by ethanol or isopropanol. Practice has shown that this procedure is not reproducible and is not very reliable for quantification. Because of the presence of alcohol some of the proteins release th ...
EF-Tu PROTEIN DOMAINS
... The bacterial elongation factor Tu (EF-Tu) has been extensively studied for decades as it plays a key role in protein biosynthesis. It is a model, multifunctional GTP-protein. This protein is also in the centre of interest as a possible target for new antibiotics. Moreover, the high homology in stru ...
... The bacterial elongation factor Tu (EF-Tu) has been extensively studied for decades as it plays a key role in protein biosynthesis. It is a model, multifunctional GTP-protein. This protein is also in the centre of interest as a possible target for new antibiotics. Moreover, the high homology in stru ...
Proc. Natl. Acad. Sci. USA
... 50,000 daltons, are found in immunoprecipitates made from 32P-labeled transformed chicken cells with antitumor antiserum (Fig. 3) (3). Neither of these is related to pp6Osrc in amino acid sequence (ref. 3; see below). Both appear to be cellular proteins that coprecipitate with pp6osrc because they a ...
... 50,000 daltons, are found in immunoprecipitates made from 32P-labeled transformed chicken cells with antitumor antiserum (Fig. 3) (3). Neither of these is related to pp6Osrc in amino acid sequence (ref. 3; see below). Both appear to be cellular proteins that coprecipitate with pp6osrc because they a ...
Protein import into yeast mitochondria van Wilpe, S.
... steady-statee level of the timl 7 R105K mutant protein was reduced to about 50% of the wild type level,, which may be directly due to reduced import efficiency of this mutant protein into mitochondria.. The amount of Tim complex in mitochondria of nine timl 7 R105K mutants variedd from 0 to 100% of ...
... steady-statee level of the timl 7 R105K mutant protein was reduced to about 50% of the wild type level,, which may be directly due to reduced import efficiency of this mutant protein into mitochondria.. The amount of Tim complex in mitochondria of nine timl 7 R105K mutants variedd from 0 to 100% of ...
chapter 3
... 3. Where stereoisomers of biomolecules are possible, only one is usually found in most organisms; for example, only the L amino acids occur in proteins. What problems would occur if, for example, the amino acids in the body proteins of herbivores were in the L isomer form, whereas the amino acids in ...
... 3. Where stereoisomers of biomolecules are possible, only one is usually found in most organisms; for example, only the L amino acids occur in proteins. What problems would occur if, for example, the amino acids in the body proteins of herbivores were in the L isomer form, whereas the amino acids in ...
Document
... tyrosine content of this protein. You know from your study of this chapter that there is a relatively easy way to do this. You prepare a pure 50 M solution of the protein, and you place it in a sample cell with a 1-cm path length, and you measure the absorbance of this sample at 280 nm in a UV-visib ...
... tyrosine content of this protein. You know from your study of this chapter that there is a relatively easy way to do this. You prepare a pure 50 M solution of the protein, and you place it in a sample cell with a 1-cm path length, and you measure the absorbance of this sample at 280 nm in a UV-visib ...
Document
... A case study - Leukocyte function associated protein-1 (LFA-1) This protein is involved in tethering a leukocyte to a endothelium, allowing migration through the tissue to a site of inflammation. One domain of LFA-1, the I-domain is 181 amino acids and undergoes a conformational change where helix ...
... A case study - Leukocyte function associated protein-1 (LFA-1) This protein is involved in tethering a leukocyte to a endothelium, allowing migration through the tissue to a site of inflammation. One domain of LFA-1, the I-domain is 181 amino acids and undergoes a conformational change where helix ...
Phase I: Pureed Foods
... Carb Solutions- 20 g protein, 4 g carb, 2 g fat (2 scoops) Designer Whey Protein (unflavored or flavored)- 20 g protein, 2 g carb, 2 g fat (1 scoop) Aria Women’s Wellness Protein Whey and Soy- 15 g protein, 5 g carb, 1 g fat (2 scoops) Designer Protein Lite (whey/soy) – 20 g protein, 10 g carb, 0 g ...
... Carb Solutions- 20 g protein, 4 g carb, 2 g fat (2 scoops) Designer Whey Protein (unflavored or flavored)- 20 g protein, 2 g carb, 2 g fat (1 scoop) Aria Women’s Wellness Protein Whey and Soy- 15 g protein, 5 g carb, 1 g fat (2 scoops) Designer Protein Lite (whey/soy) – 20 g protein, 10 g carb, 0 g ...
The Three-Dimensional Structure of the 15 Domain of the Human
... differing in the spacing between the first two cysteines (13 and 12 instead of 6 residues). The last domain of LEKTI is of particular interest, because of its partial homology to the only known natural occuring tryptase inhibitor LDTI, as well as the unequivocal correlation between the severe skin d ...
... differing in the spacing between the first two cysteines (13 and 12 instead of 6 residues). The last domain of LEKTI is of particular interest, because of its partial homology to the only known natural occuring tryptase inhibitor LDTI, as well as the unequivocal correlation between the severe skin d ...
Accuracy of protein flexibility predictions
... on B-value curves and might bias the parameters for those residues. This could be avoided by smoothing the curve, but no real effect on predictability was seen, e.g., in the case of the VTR method the unsmoothed value with window size 9 was 0.3302 while it was 0.3304 for the smoothed data. The same ...
... on B-value curves and might bias the parameters for those residues. This could be avoided by smoothing the curve, but no real effect on predictability was seen, e.g., in the case of the VTR method the unsmoothed value with window size 9 was 0.3302 while it was 0.3304 for the smoothed data. The same ...
Electrophoretic Extraction and Proteomic Characterization of
... cycling is important for tracking the global organic nitrogen cycle. Solid state NMR has provided evidence that the majority of organic nitrogen in dissolved and particulate marine organic matter contains amide bonds as found in proteins [2–4]. Protein building blocks, such as total hydrolysable ami ...
... cycling is important for tracking the global organic nitrogen cycle. Solid state NMR has provided evidence that the majority of organic nitrogen in dissolved and particulate marine organic matter contains amide bonds as found in proteins [2–4]. Protein building blocks, such as total hydrolysable ami ...
Trends in Plant Science
... of every gene product of Arabidopsis and other model plants is known. Interestingly, in addition to the holistic or ‘top-down’ approach through mutant identification and characterization, there is currently a renaissance of plant studies starting at the level of the gene product itself (a minimalist ...
... of every gene product of Arabidopsis and other model plants is known. Interestingly, in addition to the holistic or ‘top-down’ approach through mutant identification and characterization, there is currently a renaissance of plant studies starting at the level of the gene product itself (a minimalist ...
Protein Basics
... • Only 10% of the {φ, ψ} combinations are generally observed for proteins • First noticed by G.N. Ramachandran ...
... • Only 10% of the {φ, ψ} combinations are generally observed for proteins • First noticed by G.N. Ramachandran ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.