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B Ca(2+)
B Ca(2+)

... at 12,000X magnification on a JEOL 2000 EX-II 200 keV transmission electron microscope. Images were scanned using an Agfa DuoScan T2500 and adjusted for contrast using Adobe Photoshop software. ...
Amino Acids and Proteins
Amino Acids and Proteins

... protein. The structural formulas of di- and tripeptides are written. The secondary forms of protein structure include the alpha helix, pleated sheet and collagen. The interaction of side groups to form the cross-links of tertiary structure is discussed. The breakdown in the secondary and tertiary st ...
Document
Document

... – Transcription factors, splicing factors ...
Datasheet - Santa Cruz Biotechnology
Datasheet - Santa Cruz Biotechnology

... Protocadherins are a subfamily of cadherins, a large group of related glycoproteins that mediate calcium-dependent cell-to-cell adhesion via a homophilic mechanism. Involved in a variety of functions, protocadherins help to regulate neural development and synapse formation. PCDH1 (protocadherin 1), ...
Lecture PPT
Lecture PPT

... Figure 1 Generic mass spectrometry (MS)-based proteomics experiment. The typical proteomics experiment consists of five stages. In stage 1, the proteins to be analysed are isolated from cell lysate or tissues by biochemical fractionation or affinity selection. This often includes a final step of on ...
Class details
Class details

... Dye (Coomassie Brilliant Blue G-250) Dye binds protein, Abs increases (at 595nm) More protein = ? ...
Histone Deacetylase - Bio
Histone Deacetylase - Bio

... metabolism and other cellular processes. Although there is no direct evidence for the role of sirtuins in extending lifespan in mammals, various mouse models show that small molecules targeting SIRT proteins may treat age-associated diseases including diabetes, cancer and cardiac dysfunction. Signal ...
BIO2093_DMS4_sequence_similarity
BIO2093_DMS4_sequence_similarity

... • Members of a protein family have similar threedimensional structures, functions and sequence similarity. • Families can include proteins with the same function in different organisms (orthologues). • Can also include members of multigene families derived from gene duplication and ...
Chap21
Chap21

... Ways to mark proteins for degradation • In the cell, there are a couple of ways to target damaged or unwanted proteins: 1. Non-selectively, the protein interacts with a lysosome, with its numerous proteases. The process can be selective when energy levels are low and “KFERQ” proteins are targeted. ...
CYP450 Protein Assay – Human Induction Kit Extended Panel
CYP450 Protein Assay – Human Induction Kit Extended Panel

... Figure 1. 3A4, 2D6, 1A2 and 2Cs are the major isozymes that metabolise the drugs on the market. 2B6 is also important because it is inducible and drug-drug interaction potential. 2D6 is thought not to be inducible and therefore not typically studied. ...
Welcome to Biochemistry/Endocrinology
Welcome to Biochemistry/Endocrinology

...  GPCRs bind to a diverse range of ligands (proteins, peptides, amino acid derivatives, lipids, nucleotides)  Play an important role in endocrine, paracrine, autocrine signaling in all tissues and cell types  Sensory proteins just like rhodopsin are GPCRs  GPCRs, their ligands and their downstrea ...
213lec6
213lec6

Diversity of proteins
Diversity of proteins

... Fibrous proteins (e.g. keratin) may be largely  -helix Globular proteins vary greatly in  -helix content: average  helix content of 26% All side chains point outward from cylinder of helix Ala often found in  helix; Pro & Gly usually not present in helix but may be at ends ...
Proteins
Proteins

... • This level of structure is called tertiary structure and is the highest level of structure that an individual polypeptide can attain. • However, many proteins require more than one chain to function. • Such proteins display quaternary structure, which can be as simple as a functional protein consi ...
INFORMATION FOR FOREIGN STUDENTS
INFORMATION FOR FOREIGN STUDENTS

... a) The properties of a protein molecule (molecular mass, shape and charge of molecules, number of hydrophobic groups); b) Environmental factors (pH, salt composition of the medium, temperature). c) Solutions of proteins have a duality: in essence they are true molecular solutions, as particles of pr ...
Uncommon amino acids, amino acids forming proteins
Uncommon amino acids, amino acids forming proteins

... determines the native, or most frequently reoccurring secondary and tertiary structures ...
P F  I
P F I

... More information about our facility can be found on our website (http://vgn.uvm.edu/proteomics) ...
Budding yeast Saccharomyces cerevisiae as a model to study
Budding yeast Saccharomyces cerevisiae as a model to study

Heterologous Protein Production in Eukaryotic Cells
Heterologous Protein Production in Eukaryotic Cells

... selectable gene marker and yeast specific transcription and translation control sequences. Its survival depends on intergration into yeast chromosomal DNA. Typically, YIp vectors integrate as a single copy. However, methods to integrate multiple copies and stable cell lines with up to 15-20 copies o ...
Intrinsic Protein Disorder and Protein
Intrinsic Protein Disorder and Protein

... The C-terminal region of p53 uses disorder to bind to more than 45 different proteins and to form a tetramer, but only six of these complexes and the tetramer have had their structures deposited in the Protein Data Bank (PDB) [15]. One particular p53 segment “SHLKSKKGQSTSRHKKLMFKTE” (residues 367-38 ...
Protein Folding
Protein Folding

exon f exon g
exon f exon g

Class: Protein functional Annotation and Family Classification
Class: Protein functional Annotation and Family Classification

... Most new protein sequences come from genome sequencing projects ...
Modeling the Structural Consequences of BEST1 Missense Mutations
Modeling the Structural Consequences of BEST1 Missense Mutations

... over 100 unique allelic variants have been linked to the human BEST1 (hBEST1), and associated with disease phenotypes, broadly termed as bestrophinopathies. A spontaneous animal model recapitulating BEST1related phenotypes, canine multifocal retinopathy (cmr), is caused by mutations in the canine ge ...
The Synthetic Machinery of the Cell
The Synthetic Machinery of the Cell

... several undesirable side effects. Prolonged use of barbiturates induced proliferation of the SER in the liver and consequent impairment of liver function. Barbiturates were used extensively in experimental animals for the study of SER. Barbiturates happens to have an unusually marked effect in induc ...
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Interactome



In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.
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