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IOBC Hasselt
IOBC Hasselt

... Biological control ...
Gene Section E2F3 (E2F transcription factor 3) Atlas of Genetics and Cytogenetics
Gene Section E2F3 (E2F transcription factor 3) Atlas of Genetics and Cytogenetics

... Genomic amplification of E2F3: FISH image shows HT-1376 bladder cancer cell line (DSMZ acc 397) hybridized with a BAC clone (RPMI-99F1) covering the E2F3 locus at 6p22.3. (See breakpoint diagram below for map.) Note high level genomic amplification comprising multiple tandemly repeated copies of E2F ...
Amino acid sequence of an immunogenic corneal stromal protein.
Amino acid sequence of an immunogenic corneal stromal protein.

... structures. The greatest difference was located in the Cterminal Ca2+-binding domain. Five consecutive amino acid changes from Dti3-K-K-G-A67 in CO-Ag to M^-QrDE-Qb7 occurred in CaG-C. These differences alter the structure of CO-Ag, which no longer can bind Ca2+ ions. The existence of this nonfuncti ...
Determining Evolutionary Relatedness Using Amino Acid and
Determining Evolutionary Relatedness Using Amino Acid and

... other. Click on IMPORT ALIGNMENT. 2. Check the box next to the CLUSTALW set (your list of organisms). Click on BOXSHADE in the toolbar. When a new page appears, go to the bottom and click on SUBMIT. 3. A beautiful colored array of amino acid sequences should appear. Completely Conserved and Partiall ...
Extension and Enrichment
Extension and Enrichment

... R. Sickle cell anemia is caused by a single change in the amino acid sequence. Amino acid 6 is Glutamic acid in normal hemoglobin. It is replaced with Valine in sickle cell anemia. Using the information you just learned, explain why sickle cell anemia leads to deformed blood cells. ...
Protein Structure - Oregon State University
Protein Structure - Oregon State University

Chapter 14: Carbohydrates
Chapter 14: Carbohydrates

... • The nonapeptides oxytocin and vasopressin have similar 1o structures. • Only the amino acids at positions 3 and 8 differ. ...
Slide 1
Slide 1

... • Like DNA polymerases, RNA polymerases can add nucleotides only to the 3’ end of the growing polymer. • Specific sequences of nucleotides along the DNA mark where gene transcription begins and ends. – RNA polymerase attaches and initiates transcription at the promotor ‫ال ُم َحفـز‬, at the beginnin ...
XBRAINоаWhey Protein Isolate Worlds finest Only the Finest Quality
XBRAINоаWhey Protein Isolate Worlds finest Only the Finest Quality

... percent of the protein content.  Whey as a group of proteins is the fraction that separates out during cheese making. This fraction is purified to different concentrations depending on the end­product desired and varies in protein, lactose, immunoglobulins, minerals and fat content.  Of all protein ...
RNA polymerase I
RNA polymerase I

... the operon model for the control of gene expression in bacteria. • An operon consists of three elements: – the genes that it controls, • In bacteria, the genes coding for the enzymes of a particular pathway are clustered together and transcribed (or not) as one long mRNA molecule. – a promoter regio ...
Ch. 10: Presentation Slides
Ch. 10: Presentation Slides

... • tRNAs are covalently attached to specific amino acids by aminoacyl- synthetases and contain anti-codon complementary to the mRNA codon • Base pairing between the tRNA anti-codon and the mRNA codon on the ribosome places amino acids in the correct linear sequence in translation ...
Dancing Naked in the Mind Field
Dancing Naked in the Mind Field

... 1)Gene of interest (GFP) is isolated and prepared by removal of introns 2)An appropriate plasmid vector is chosen 3)GFP gene and plasmid are each treated with the same restriction enzymes to make identical cuts 4)The DNA fragments are mixed together and complimentary ends are attracted by base-pairi ...
Ch 16+ 17 Reading Guide
Ch 16+ 17 Reading Guide

... 1. Describe Beadle and Tatum's experiments with Neurospora and explain the contribution they made to our understanding of how genes control metabolism. 2. Explain how RNA differs from DNA. 3. Distinguish between transcription and translation. 4. Define “codon” and explain the relationship between th ...
Self-Interaction of the Herpes Simplex Virus Type 1
Self-Interaction of the Herpes Simplex Virus Type 1

Communicating Research to the General Public
Communicating Research to the General Public

... interactions between their membrane components. This means that to understand the natural interaction tendencies of these two proteins, I needed to study them in an environment that is close to the composition of the cell membrane. Now, the cell membrane's composition is very diverse – it is made up ...
Synaptonemal complex proteins - Journal of Cell Science
Synaptonemal complex proteins - Journal of Cell Science

... screened by affinity elution (Sambrook et al., 1989). The eluted antibodies were immune tested on spermatocytes in order to detect those phage clones that have a cDNA insert that produces meiotic chromosome antigens. The inserts of choice were excised in vivo as Bluescript plasmids following the man ...
Identification of a Fluorescent Protein from Rhacostoma Atlantica
Identification of a Fluorescent Protein from Rhacostoma Atlantica

7.013 Problem Set 1 - MIT OpenCourseWare
7.013 Problem Set 1 - MIT OpenCourseWare

... a six amino acid linker. What is the secondary structure of this amino acid linker (choose from helices, sheets and coils)? Note Click on “Reset - > Reset structure”. Then click on “Structure -> Protein-> Secondary”. Select helices, sheets and ribbons one at a time and look at the amino acid sequenc ...
Spotlight on Metabolism
Spotlight on Metabolism

... •Refusal to maintain body wt at or above a min wt for age/ height: • Body weight less than 85% of that expected. •Intense fear of wt gain or becoming fat, even though under wt. •Disturbance in the way one's body shape are experienced, •Denial of the seriousness of the current low body weight. •Ameno ...
Protein Structure Prediction
Protein Structure Prediction

Exosome Complex www.AssignmentPoint.com The exosome
Exosome Complex www.AssignmentPoint.com The exosome

... The core of the exosome contains a six-membered ring structure to which other proteins are attached. In eukaryotic cells, the exosome complex is present in the cytoplasm, nucleus and especially the nucleolus, although different proteins interact with the exosome complex in these compartments regulat ...
Genomic organization of infectious salmon anaemia virus
Genomic organization of infectious salmon anaemia virus

... Recent evidence shows that ISAV is similar to orthomyxoviruses in that it binds to sialic acid residues on host cell surfaces and undergoes fusion with the cell in acidic endosomes (Eliassen et al., 2000). The haemagglutinating and acetylesterase activities seem to be carried out by two different pr ...
Organic Molecules
Organic Molecules

... sequence of amino acids. ...
Role of Water Mediated Interactions in Protein
Role of Water Mediated Interactions in Protein

... however. One reason for this is that water takes on numerous different roles. Among other roles, water participates in many specific interactions, screens efficiently Coulombic interactions, mediates proton transfer,3 and even is used as a structural component in protein secondary structure.4 When t ...
Trimer Codon Mix 2 Antisense
Trimer Codon Mix 2 Antisense

... Description Directed molecular evolution and combinatorial methods are key strategies used for protein engineering research. These approaches commonly involve using partially randomized synthetic oligonucleotides to generate a partially randomized gene library, expressing it in an appropriate vector ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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